Effects of Different Nitrogen and Phosphorus Synergistic Fertilizer on Enzymes and Genes Related to Nitrogen Metabolism in Wheat

In recent years,in order to improve nutrient use efficiency,especially nitrogen use efficiency,fertilizer valueadded technology has been developed rapidly.However,the mechanism of the effect of synergistic fertilizer on plant nitrogen utilization is not clear.A study was,therefore,conducted to explore the activities and gene expression of key enzymes for nitrogen assimilation and the gene expression of nitrogen transporters in wheat after the application of synergistic fertilizer.Soil column experiment was set up in Qingdao Agricultural University experimental base from October 2018 to June 2019.Maleic acid and itaconic acid were copolymerized with acrylic acid as cross-linking monomer to make a fluid gel,which was sprayed on the fertilizer surface to make nitrogen and phosphorus synergistic fertilizer.A total of 6 treatments was set according to different nitrogen and phosphorus fertilizer ratios:(1)100%common nitrogen fertilizer+100%common phosphate fertilizer(2)70%nitrogen synergistic fertilizer+100%phosphorus synergistic fertilizer;(3)100%nitrogen synergistic fertilizer+70%phosphorus synergistic fertilizer;(4)100%nitrogen synergistic fertilizer+100%phosphorus synergistic fertilizer;(5)70%nitrogen synergistic fertilizer+70%phosphorus synergistic fertilizer;(6)100%commercial nitrogen synergistic fertilizer+100%commercial phosphorus synergistic fertilizer.The results are as follows:(1)the enzyme activities of wheat plants under synergistic fertilizer condition were higher than those under ordinary fertilizer,except under the treatment that nitrogen and phosphorus synergistic fertilizer were both reduced;(2)the expression level of the genes under the treatment“100%nitrogen synergistic fertilizer+100%phosphorus synergistic fertilizer”was significantly higher than those in other treatments.Combined with the higher performance of nitrogen concentration in various parts of the plant under the condition of applying synergistic fertilizer,this study indicated that the application of synergistic fertilizer can improve the nitrogen metabolism of the plant by increasing the nitrogen level in the rhizosphere soil,inducing the expression of nitrogen transporter genes and key assimilation enzymes genes.

Effects of Zinc on the Yield,Uptake and Distribution of Nitrogen/Phosphorus/Potassium/Zinc in Different Genotypes of Maize

[Objective] To study the effects of Zinc on the yield,nitrogen/phosphorus/potassium/Zinc uptake and distribution of different genotypes of maize.[Method] Using two different genotypes of maize as materials,the effects of Zn on the yield,N/P/K /Zn uptake and distribution of different genotypes of maize were studied by pot cultivation.[Result] Appropriate Zn supply could improve the grain number per kernel.The Zn content in different organs of maize showed little difference under low Zn treatment （Zn0,Zn1）.With the increase of Zn supply,Zn content in leaf,stem and sheath rapidly increased,but the increasing amplitude of Zn content in the kernel and bract were less.The excessive Zn in maize was mainly translocated in lower organs to reduce the damage of them to plants.Different Zn supply levels had less effects on the absorption and translocation of N,P and K in low-Zn insensitive variety Mudan 9.While the absorption and translation of N,P,K in low Zn sensitive variety （Sidan 19） was more easily influenced by the amount of Zn supplied.[Conclusion] Appropriate Zn supply could enhance the maize yield and utilization rate of N and K,and reduce the absorption and utilization of P.

Effect Models of Nitrogen,Phosphorus and Potassium Fertilizer Formulation in Banana

[Objective] The aim was to modify the application amount of N,P and K fertilizer so as to provide a reference for establishing balanced fertilization index system of banana.[Method]The N,P and K fertilizer ＂3414＂ test was carried out on banana,and then regression analysis was performed on the fertilizer effect.Ternary quadratic,binary quadric and one-variable quadratic regression equations for the fertilizer effect on the banana yield were constructed.[Result]Suitable amount of N,P and K fertilizer had significant yield improving effect,whereas overdose of fertilizer application led to decreasing of utilization rate of fertilizer.Therefore,suitable amount of N,P and K fertilizer should be selected in production.It could be concluded that one-variable quadratic regression equations was the best model to calculate the suitable fertilizer amount.The best yield range of banana in the tested field was 44.193-45.904 t/hm2,while the corresponding optimum application amount of N,P2O5 and K2O was 795.1,262.3 and 1 236.9 kg/hm2 respectively,and the ratio among nitrogen,phosphorus and potassium are 1∶0.33∶1.55.[Conclusion]The result in this study could provide references for the soil types similar to the tested field.

Study on Absorption Law of Nitrogen,Phosphorus and Potassium and Proper Nitrogen Application in Chuanxiangyou 9838 under No-tillage Cultivation

The aim of the study is to investigate the absorption laws of nitrogen, phosphorus, and potassium, and proper nitrogen application in Chuanxiangyou 9838 under no-tillage cultivation. Five nitrogen application treatments were designed to analyze the absorption laws of N, P and K, and to discuss the effects of different N fertilizer application amounts on yield and yield composition factors of Chuanxiangyou 9838. The results showed that gross nutrient absorption in Chuanxiangyou 9838 was greatly varied at different developmental stages under rice-rape rotation with no-tillage. The maximum N absorption in Chuanxiangyou 9838 appeared at jointing stage followed by heading stage, thirdly the tillefing stage ; the P absorption in Chuanxiangyou 9838 presented the consecutively slight increase during seedling stage and mature stage ; the K absorption in Chuanxiangyou 9838 was mainly conducted from jointing stage to heading stage, during which K absorption accounts for 73.4% of the total absorption in whole developmental stage. Consequently, N fertilizer should be applied earlier （ before jointing stage）, P fertilizer is suitable as base fertilizer and application of K fertilizer should be preferably conducted at early-middle period. When the yield reached 11 t/hm^2, the optimal N application amount in Chuanxiangyou 9838 was about 165 kg/hm^2.

Effects of Nitrogen, Phosphate and Potassium Fertilizers on Soft Rot of Konjac Based on Grey Correlation Analysis

[Objective] The effects of different application amounts of nitrogen, phos-phate and potassium fertilizers on soft rot of konjac were investigated in this study. [Method] The grey correlation analysis was adopted to evaluate the correlation be-tween the application amount of nitrogen, phosphate and potassium fertilizers and the occurrence of soft rot of konjac. [Result] The excessive application of nitrogen fertilizer would induce the occurrence of soft rot of konjac, but the application of potassium fertilizer had a good control effect on soft rot. [Conclusion] The applica-tion amount of nitrogen fertilizer should be control ed reasonably in the planting of konjac, but the application amount of phosphate and potassium fertilizer could be in-creased to some extent, reducing the occurrence of soft rot of konjac.

Transgenic approaches for improving use efficiency of nitrogen, phosphorus and potassium in crops

The success of the Green Revolution largely relies on fertilizers, and a new Green Revolution is very much needed to use fertilizers more economically and efficiently, as well as with more environmental responsibility. The use efficiency of nitrogen, phosphorus, and potassium is controlled by complex gene networks that co-ordinate uptake, re-distribution, assimilation, and storage of these nutrients. Great progress has been made in breeding nutrient-efficient crops by molecularly engineering root traits desirable for efficient acquisition of nutrients from soil, transporters for uptake, redistribution and homeostasis of nutrients, and enzymes for efficient assimilation. Regulatory and transcription factors modulating these processes are also valuable in breeding crops with improved nutrient use efficiency and yield performance.

Impact of Nitrogen, Phosphorus and Potassium on Brown Planthopper and Tolerance of Its Host Rice Plants

The brown planthopper(BPH),Nilaparvata lugens(St?l),appeared as a devastating pest of rice in Asia. Experiments were conducted to study the effects of three nutrients,nitrogen(N),phosphorus(P) and potassium(K),on BPH and its host rice plants. Biochemical constituents of BPH and rice plants with varying nutrient levels at different growth stages,and changes in relative water content(RWC) of rice plants were determined in the laboratory. Feeding of BPH and the tolerance of rice plants to BPH with different nutrient levels were determined in the nethouse. Concentrations of N and P were found much higher in the BPH body than in its host rice plants,and this elemental mismatch is an inherent constraint on meeting nutritional requirements of BPH. Nitrogen was found as a more limiting element for BPH than other nutrients in rice plants. Application of N fertilizers to the rice plants increased the N concentrations both in rice plants and BPH while application of P and K fertilizers increased their concentrations in plant tissues only but not in BPH. Nitrogen application also increased the level of soluble proteins and decreased silicon content in rice plants,which resulted in increased feeding of BPH with sharp reduction of RWC in rice plants ultimately caused susceptible to the pest. P fertilization increased the concentration of P in rice plant tissues but not changed N,K,Si,free sugar and soluble protein contents,which indicated little importance of P to the feeding of BPH and tolerance of plant against BPH. K fertilization increased K content but reduced N,Si,free sugar and soluble protein contents in the plant tissues which resulted in the minimum reduction of RWC in rice plants after BPH feeding,thereby contributed to higher tolerance of rice plants to brown planthopper.

Nitrogen, phosphorus and potassium recycling in an agroforestry ecosystem of Huanghuaihai Plain: with Paulownia elongata intercropped wheat and maize as an example

The studies show that in the whole community, P is deficient, and N and K are basically balanced. N, P and K are accumulated in plant tissues and litters, but depleted in soil. N and P contents in surface soil(0—20 cm) are the main factors affecting crop growth, and P contents in 20 80 cm soil layer is the major affecting Paulownia elongata growth. The absorption coefficients of N, P and K in the communities are 0 078, 0 014 and 0 052 respectively, their utilization coefficients are 0 95, 0 90 and 0 94, and the recycling coefficients are 0 042, 0 05 and 0 063 respectively.

Effects of Nitrogen-phosphorus-potassium Combined Fertilization on Rice Yield and Fertilizer Use Efficiency in Jianghan Plain

[Objectives]This study was conducted to explore the rational formula for rice fertilization in Jianghan Plain.[Methods]An experiment on the combined application of nitrogen,phosphorus and potassium fertilizers was carried out in Jianghan Plain,an important rice producing area in Hubei,with a total of five treatments to study the effects of nitrogen,phosphorus and potassium fertilizers on the fertilizer use efficiency and yield of rice.[Results]Fertilization had a significant effect on improving rice yield,and nitrogen fertilizer had the greatest effect on rice yield,followed by potassium fertilizer and phosphorous fertilizer.[Conclusions]This study provides a scientific basis for the application of rice fertilizers and the reduction and efficiency improvement of chemical fertilizers in Jianghan Plain.

Production Benefits of Double-Cropping Rice Under Optimized Application of Nitrogen and Potassium Fertilizers Combined with Chinese Milk Vetch and Straw Co-Returning to Fields

In order to explore the technology and effects of reducing nitrogen and potassium fertilizer applications in double-cropping rice,a field plot experiment was conducted to study the effects of optimized application of nitrogen and potassium fertilizers combined with returning Chinese milk vetch and straw to fields on yield,fertilizer utilization efficiency,net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci),chlorophyll content(SPAD value)and soil physical and chemical properties in late rice harvest period.The results showed that the optimized application of nitrogen and potassium fertilizers combined with the integrated technology of Chinese milk vetch and straw co-returning to the field could enhance the photosynthetic efficiency of double-cropping rice,increase rice yield,and enhance soil biological activity,especially T4 treatment involving the returning of Chinese milk vetch and straw to the field instead of 30%nitrogen fertilizer achieved the highest rice yield,fertilizer use efficiency,net photosynthetic rate and soil biological activity.Compared with the conventional fertilization treatment T2,the total rice yield of T4 treatment increased by 4.1%,among which the early rice and late rice increased by 6.3%and 2.4%,respectively;Pn,Gs and SPAD values of flag leaves at full heading stage significantly increased,and the contents of soil active organic carbon,alkali hydrolyzed nitrogen,available phosphorus and readily available potassium significantly increased.

Effects of a Combined Application of Nitrogenous and Phosphorus Fertilizers on the Growth of Pinus radiata

In the arid valley of the upper reaches of the Minjiang river,combined application tests of nitrogenous and phosphorus fertilizers were conducted to the 1-year-old seedlings of Pinus radiate to investigate the appropriate amount of fertilization,so as to provide bases of fertilization management of young P.radiate in the arid val-ley.Different combined application amounts of nitrogenous and phosphorus were studied based on the randomized blocks design.The results showed that in comparing 3 treatment groups with the control,the first and second treatments significantly increased the ground diameter and height of P.radiate,while the third treatment merely significantly increased the ground diameter.Under the conditions of this study,the recommended fertilization amount was 30 g of urea and 150 g of calcium superphosphate per plant for the one-year-old P.radiate.

Evolution of Nitrogen, Phosphorus and Potassium Fertilizer Application Rates in Cotton Fields and Its Influences on Cotton Yield in the Yangtze River Valley

[Objective] The historical evolution pattern of nitrogen （N）, phosphorus （P） and potassium （K） fertilizer application rate and its effects on lint cotton yield were explored to provide the theoretical basis for reasonable fertilizer management strate-gy in the cotton planting region of the Yangtze River Val ey. [Method] GGE biplot analysis method was adopted to analyze the correlation among N, P and K fertilizer application rate and lint cotton yield with the dataset of national cotton regional trials of the Yangtze River Val ey during 1991-2013. The linear and nonlinear regression analysis method was used to reveal the evolution of the fertilizer applying patterns, and analyze the effects of N, P, K application rates on cotton lint yield. [Result] The application rates of N, P and K fertilizer presented highly significant positive corre-lation with lint cotton yield, among which the potassium fertilizer was the strongest relative factor with lint cotton yield, fol owed by phosphorus fertilizer, while nitrogen fertilizer was the weakest factor. The application rate of nitrogen fertilizer was relat-ed with the test year in the pattern of a quadratic function, while phosphate and potassium had progressive increase linear relation with the test year in the cotton planting region of the Yangtze River Val ey. Meanwhile, cotton lint yield was in re-sponse to nitrogen fertilizer content increase with a quadratic parabola function, and increased with the applying phosphate fertilizer and potassium fertilizer content with linearly increasing function. [Conclusion] The increasing application amount of N, P and K fertilizer was general y beneficial to cotton yield improvements, however, ex-orbitant applying nitrogen fertilizer was unfavorable for cotton production, and a reasonable mixture formula of N, P and K fertilizer was better in terms of cotton yield-increasing effect.

Effects of Controlled Release Fertilizer on Loss of Nitrogen and Phosphorus from Farmland

[Objective] The aim was to study on effects of controlled release fertilizer on loss of nitrogen and phosphorus from farmland. [Method] Experiment was conducted in fields planted with rice and corn around Chao Lake and effects of compound fertilizer, controlled release fertilizer and controlled release fertilizer （reduced by 20%） on loss of nitrogen and phosphorus through runoff and leaching were analyzed. [Result] Loss of nitrogen and phosphorus mainly occurred in early stage of fertilizing; loss caused by runoff accounted for over 98% and caused by leaching was lower than 2%, indicating that nutrients of rice and corn mainly lost through runoff. As for controlled release fertilizers with 20% reduced, total loss of N and P decreased by 60% and 63% in rice field and reduced by 27.8% and 34% in corn field, respectively, indicating that controlled release fertilizer would maintain nutrients in soils high in later period of plant growth, improve use efficiency of N and P, reduce N and P loss in rice and corn fields in rainy season, and decrease non-point pollution. [Conclusion] The research suggested that controlled release fertilizer would slow down the loss of nutrients in farmlands, providing scientific references and technological support for extension of controlled release fertilizer and reduction of agricultural non-point pollution.

Nitrogen and Phosphorus Loss Law and Emission Reduction Effects Under Water and Fertilizer Management Integrated Mode in Dike Paddy Field

To achieve the purpose of reducing farm non-point source pollution, we integrated site specific nitrogen management precise irrigation, controlled drainage, and wetland eco-repair system in dike area of Taihu basin. During investigation, it had given prominence for the water and fertilizer coupling effects of precise irrigation and site specific nutrient management, the characteristics of integration on controlled irrigation, controlled drainage and wetland ecosystem non-point source pollution control. Then the water and fertilizer integrated management mode of paddy field was put forward in Taihu basin where the water production efficiency increased to 1.64 kg. m-3, water saved 37.8%, fertilizer use efficiency raised 15,4%, yield raised 10%, and N, P load decreased 26%-72%. The modern agricultural and farmland ecosystems that control and cut down the farm non-point source pollution came into being, which can be a reference by Taihu basin to control its agricultural non-point source pollution and eutrophicated water body.

Effect of nitrogen fertilizer on the uptake and utilization of potassium by various rice varieties in purple soil

Nitrogen and potassium are important nutrientelements for rice.Besides supplied by the or- ganic manure,potassium nutrition of ricecomes dominantly from purple soils in Sichuanbasin.Potassium exists abundantly in mineralforms in the purple soils.Availability of soilpotassium for crop depends on the potassiumforms,the uptake ability of crops,and fertiliz-er practices.A pot culture experiment wasconducted to study the kinetics of potassiumuptake in the purple soil(total N 22.64g·kg,total K 24.36g·kg,available N 102.6mg·kg,available K 140.6mg·kg,acidsoluble K 936.0mg·kg,and pH 6.8).Ma-terials used were three Fhybrid rices,Eryou

The Position of Mineral Nitrogen Fertilizer in Efficient Use of Nitrogen and Land:A Review

Our attitude towards mineral nitrogen (N) fertilizers is ambivalent. N fertilizers have on one hand increased our supply of food, feed and other bio-based raw materials tremendously and also improved the use efficiency of land and labor, but have on the other hand a negative impact on the quality of the environment and contributed to the depletion of fossil fuel reserves. This awareness has resulted in strong pleas to spend much more attention to the recycling of N containing downstream “wastes”. It is, however, naive to assume that even perfect recycling suffices to offer the same number of people the same diet without inputs of “new” N, as inevitable losses of N make compensations indispensable. “New” N can be derived from either biological N fixation (“legumes”) or from industrially fixed N (“fertilizer”). The existing literature provides no evidence that the use of N fertilizers is per se unsustainable, as these fertilizers can also be made from renewable forms of energy. Besides, soil health and human health appear sensitive for the dosage but not for the form of N. It is yet imperative to reduce the input of “new” N as much as possible, so as to minimize adverse environmental effects. Measures to this end are a more precise assessment of crop N requirements, a better timing and positioning of N inputs, and any measure supporting the acceptance of “wastes” by farmers. The present paper elaborates the above aspects.

Carbon and nitrogen allocations in corn grown in Central and Northeast China: different responses to fertilization treatments

In order to reveal the impact of various fertilization strategies on carbon（C） and nitrogen（N） accumulation and allocation in corn（Zea mays L.）, corn was grown in the fields where continuous fertilization management had been lasted about 18 years at two sites located in Central and Northeast China（Zhengzhou and Gongzhuling）, and biomass C and N contents in different organs of corn at harvest were analyzed. The fertilization treatments included non-fertilizer（control）, chemical fertilizers of either nitrogen（N）, or nitrogen and phosphorus（NP）, or phosphorus and potassium（PK）, or nitrogen, phosphorus and potassium（NPK）, NPK plus manure（NPKM）, 150% of the NPKM（1.5NPKM）, and NPK plus straw（NPKS）. The results showed that accumulated C in aboveground ranged from 2 550–5 630 kg ha^–1 in the control treatment to 9 300–9 610 kg ha^–1 in the NPKM treatment, of which 57–67% and 43–50% were allocated in the non-grain organs, respectively. Accumulated N in aboveground ranged from 44.8–55.2 kg ha^-1 in the control treatment to 211–222 kg ha^–1 in the NPKM treatment, of which 35–48% and 33–44% were allocated in the non-grain parts, respectively. C allocated to stem and leaf for the PK treatment was 65 and 49% higher than that for the NPKM treatment at the both sites, respectively, while N allocated to the organs for the PK treatment was 18 and 6% higher than that for the NPKM treatment, respectively. This study demonstrated that responses of C and N allocation in corn to fertilization strategies were different, and C allocation was more sensitive to fertilization treatments than N allocation in the area.

Plant Nutrient Status during Boll Development and Seed Cotton Yield as Affected by Foliar Application of Different Sources of Potassium

Bt cotton hybrids require large supply of metabolites to support their greater boll load and commonly suffer from premature leaf senescence. A field experiment was conducted to study the nutritional status of Bt cotton leaves during boll development stage and to evaluate the most profitable source of foliar fertilizers. Treatments included basal application of 0 and 60 kg·K2O·ha-1 as muriate of potash (MOP) in main plots and foliar spray treatments viz: 4 & 6 sprays of 2% potassium nitrate (Multi-K, 13-00-45), 4 & 6 sprays of NPK Blend (Polyfed, 19-19-19), 4 sprays of MOP, 4 sprays of MOP + urea (to supply same amount of N & K as in potassium nitrate) and unsprayed control in sub plots. The results revealed that only N and K contents of premature senesced leaves were below the sufficiency range for cotton sufficient levels of P, Fe, Mn, Zn and Cu were observed. Though the concentrations of N and K in both the petiole and leaf blade initially improved with foliar spray, N content declined below the unsprayed control at later stages. Basal application of MOP increased seed cotton yield by 19%. Four foliar sprays of KNO3, NPK, MOP and MOP + urea recorded yield increase in seed cotton yield of 22.8%, 22.4%, 18.5% and 24.5%, respectively over unsprayed control. Six sprays of KNO3 and NPK had no yield advantage over four sprays and rather proved economically less viable.

Cottonseed yield and its quality as affected by mineral fertilizers and plant growth retardants

The increase in the population in Egypt makes it imperative to explore promising approaches to increase food supply, including protein and oil, to meet the needs of the Egyptian people. Cotton is the principal crop of Egyptian agriculture, it is grown mainly for its fiber, but cottonseed products are also of economic importance. Cottonseed is presently the main source of edible oil and meal for livestock in Egypt. Economic conditions in modern agriculture demand high crop yields in order to be profitable and consequently meet the high demand for food that comes with population growth. Oil crop production can be improved by development of new high yielding varieties, and the application of appropriate agronomic practices. There is limited information about the most suitable management practice for application of N, P, K, Zn, Ca and PGRs in order to optimize the quantity and quality of oil and protein of cottonseed. In maximizing the quantity and quality of a crop’s nutritional value in terms of fatty acids and protein, field experiments were conducted to investigate the effect of nitrogen, phosphorus, potassium, foliar application of zinc and calcium, the use of plant growth retardants (Pix, Cycocel or Alar), on cottonseed, protein, oil yields, and oil properties of Egyptian cotton. From the findings of this study, it seems rational to recommend that application of N, P, K, foliar application of Zn and Ca, the use of PGRs (Pix, Cycocel or Alar), could bring about better impact on cottonseed yield, seed protein content, oil and protein yields, oil refractive index, unsaponifiable matter, and unsaturated fatty acids in comparison with the ordinary cultural practices adopted by Egyptian cotton producers.

Macronutrients Uptake in Soybean as Affected by <i>Bradyrhizobium japonicum</i>Inoculation and Phosphorus (P) Supplements

Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kg P·ha-1. Both treatments were replicated four times in a split plot design. The macronutrients considered were N, P, K, Ca and Mg. Results showed that inoculation with B. japonicum significantly contribute to the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole soybean plant. Likewise, phosphorus supplementation significantly enhanced the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole plant. The use of effective strains of rhizobia and P supplementation was an effective way of enhancing the growth of soybean, eventually the uptake of macronutrients in plant organs.