Objective: To investigate the possible effect of nivalenol on metabolism ofthe cultured chondrocytes and the protection of selenium. Methods: The quantitative analyses ofmetabolism in single- layer cultured chondrocyt...Objective: To investigate the possible effect of nivalenol on metabolism ofthe cultured chondrocytes and the protection of selenium. Methods: The quantitative analyses ofmetabolism in single- layer cultured chondrocytes were performed by biocliemical means and theimpairment of DNA was observed by both of the single cell microgel electrophoresis assay and theagarose gel electrophoresis assay. Results: In the media containing different concentrations ofnivalenol (0. 000 5-0. 020 0 mg/L), the amounts of DNA and proteoglycan in matrix of thechondrocytes were decreased. The syn-thesis of protein was reduced and the impairment of DNAdeteriorated with the increase of the concentrations of nivalenol in tlte given dose. When seleniumwas added into the media, the impairment by nivalenol was decreased. In the media containingdifferent concentrations of nivalenol, however, the lipid peroxidation of the chondrocytes was notaffected by nivalenol, yet the amount of lipid peroxides significantly declined. Conclusion:Nivalenol may evidently cause impairment of the chondrocytes when its concentrations are in thepresent experimental range. Selenium can protect cultured cliondrocytes, but cannot prevent theirDNA from being impaired.展开更多
Objective To investigate the effects of nivalenol(NIV) and selenium(Se) on the metabolism of aggrecan in the cultured chondrocytes,and to explore the mechanism involved in cartilage aggrecan catabolism in the process ...Objective To investigate the effects of nivalenol(NIV) and selenium(Se) on the metabolism of aggrecan in the cultured chondrocytes,and to explore the mechanism involved in cartilage aggrecan catabolism in the process of Kashin-Beck Disease(KBD).Method Aggrecan mRNA expression was studied by RT-PCR.The concentration of GlcUA in culture medium was determined by diphenylenimine-sulfuric acid method.Result NIV significantly decrease aggrecan mRNA expression.That Se can partially antagonize the effect of NIV on aggrecan mRNA expression.The content of GlcUA in medium with NIV was high er than that in other groups.Conclusion NIV could inhibit chondrocyte synthesis aggrecan,promote the loss of aggrecan from cartilage.All the effects result in the metabolic disorder of the cartilage aggrecan,which eventually leads to irreversible mechani cal destruction of the cartilage.It suggested that Se can partially alleviate the effects of NIV on chondrocytes cultured in vitro.展开更多
From this article,we can find that nivalenol could damage the DNA,disturb aggrecan mRNA expression in chondro cytes and further induce metabolic disturbance of cartilage extracellular matrix,which may play an importan...From this article,we can find that nivalenol could damage the DNA,disturb aggrecan mRNA expression in chondro cytes and further induce metabolic disturbance of cartilage extracellular matrix,which may play an important role during patho genesis of KBD.Se supplementation can resist the injury of nivalenol to chondrocytes,but its action is limited.展开更多
Assessing diaphragm function status is vital for diagnosing and treating acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Diaphrag-matic ultrasound has become increasingly important due to its non-i...Assessing diaphragm function status is vital for diagnosing and treating acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Diaphrag-matic ultrasound has become increasingly important due to its non-invasive nature,absence of radiation exposure,widespread availability,prompt results,high accuracy,and repeatability at the bedside.The diaphragm is a crucial respiratory muscle.Decline or dysfunction of the diaphragm can lead to dyspnea and even respiratory failure in AECOPD patients.In this editorial,we comment on an article,retrospectively analyzed ninety-four acute exacerbations of chronic obstructive pulmonary disease patients who received mechanical ventilation from January 2022 to December 2023.The study found that the diaphragm thickening fraction,an index from diaphragm ultrasound,can better predict the outcome of non-invasive ventilation in patients with AECOPD.The value of non-invasive ventilation in treating respiratory failure caused by AECOPD has been widely acknowledged.Diaphragmatic dysfunction diagnosed with ultrasound is asso-ciated with prolonged mechanical ventilation and weaning times and higher mortality.展开更多
基金Supported by the National Natural Science Foundation of China(30170831)
文摘Objective: To investigate the possible effect of nivalenol on metabolism ofthe cultured chondrocytes and the protection of selenium. Methods: The quantitative analyses ofmetabolism in single- layer cultured chondrocytes were performed by biocliemical means and theimpairment of DNA was observed by both of the single cell microgel electrophoresis assay and theagarose gel electrophoresis assay. Results: In the media containing different concentrations ofnivalenol (0. 000 5-0. 020 0 mg/L), the amounts of DNA and proteoglycan in matrix of thechondrocytes were decreased. The syn-thesis of protein was reduced and the impairment of DNAdeteriorated with the increase of the concentrations of nivalenol in tlte given dose. When seleniumwas added into the media, the impairment by nivalenol was decreased. In the media containingdifferent concentrations of nivalenol, however, the lipid peroxidation of the chondrocytes was notaffected by nivalenol, yet the amount of lipid peroxides significantly declined. Conclusion:Nivalenol may evidently cause impairment of the chondrocytes when its concentrations are in thepresent experimental range. Selenium can protect cultured cliondrocytes, but cannot prevent theirDNA from being impaired.
基金supported by the Scientific and Technological Research Project of the Education Department of Shaanxi Province(12JK0703)the Natural Science Foundation of Shaanxi Provincial Health Department(2012D75)foundation of Xi'an Medical University(11FZ26)
文摘Objective To investigate the effects of nivalenol(NIV) and selenium(Se) on the metabolism of aggrecan in the cultured chondrocytes,and to explore the mechanism involved in cartilage aggrecan catabolism in the process of Kashin-Beck Disease(KBD).Method Aggrecan mRNA expression was studied by RT-PCR.The concentration of GlcUA in culture medium was determined by diphenylenimine-sulfuric acid method.Result NIV significantly decrease aggrecan mRNA expression.That Se can partially antagonize the effect of NIV on aggrecan mRNA expression.The content of GlcUA in medium with NIV was high er than that in other groups.Conclusion NIV could inhibit chondrocyte synthesis aggrecan,promote the loss of aggrecan from cartilage.All the effects result in the metabolic disorder of the cartilage aggrecan,which eventually leads to irreversible mechani cal destruction of the cartilage.It suggested that Se can partially alleviate the effects of NIV on chondrocytes cultured in vitro.
基金supported by the Scientific and Technological Research Project of the Education Department of Shaanxi Province(12JK0703)the Natural Science Foundation of Shaanxi Provincial Health Department(2012D75)the foundation of Xi’an Medical University(11FZ26)
文摘From this article,we can find that nivalenol could damage the DNA,disturb aggrecan mRNA expression in chondro cytes and further induce metabolic disturbance of cartilage extracellular matrix,which may play an important role during patho genesis of KBD.Se supplementation can resist the injury of nivalenol to chondrocytes,but its action is limited.
文摘Assessing diaphragm function status is vital for diagnosing and treating acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Diaphrag-matic ultrasound has become increasingly important due to its non-invasive nature,absence of radiation exposure,widespread availability,prompt results,high accuracy,and repeatability at the bedside.The diaphragm is a crucial respiratory muscle.Decline or dysfunction of the diaphragm can lead to dyspnea and even respiratory failure in AECOPD patients.In this editorial,we comment on an article,retrospectively analyzed ninety-four acute exacerbations of chronic obstructive pulmonary disease patients who received mechanical ventilation from January 2022 to December 2023.The study found that the diaphragm thickening fraction,an index from diaphragm ultrasound,can better predict the outcome of non-invasive ventilation in patients with AECOPD.The value of non-invasive ventilation in treating respiratory failure caused by AECOPD has been widely acknowledged.Diaphragmatic dysfunction diagnosed with ultrasound is asso-ciated with prolonged mechanical ventilation and weaning times and higher mortality.
