No-touch isolation technique in emergency pancreaticoduodenectomy for neoplastic hemorrhage: Two case reports and review of literature

BACKGROUND Emergency pancreaticoduodenectomy(EPD)is a rare event for complex periam-pullary etiology.Increased intraoperative blood loss is correlated with poor post-operative outcomes.CASE SUMMARY Two patients underwent EPD using a no-touch isolation technique,in which all arteries supplying the pancreatic head region were ligated and divided before manipulation of the pancreatic head and duodenum.The operative times were 220 and 239 min,and the blood loss was 70 and 270 g,respectively.The patients were discharged on the 14^(th) and 10^(th) postoperative day,respectively.Thirty-two patients underwent EPD for the treatment of neoplastic bleeding.The mean operative time was 361.6 min,and the mean blood loss was 747.3 g.The comp-lication rate was 37.5%.The in-hospital mortality rate was 9.38%.CONCLUSION The no-touch isolation technique is feasible,safe,and effective for reducing intraoperative blood loss in EPD.

Refining adipose-derived stem cell isolation for optimal regenerative therapy

This article highlights the importance of optimizing the techniques used for isolating stromal vascular fraction cells from adipose tissue.Furthermore,by presenting key findings from the literature,it clarifies the effects of refined techniques on regenerative medicine and advocates for ongoing research and innovation to enhance therapeutic outcomes.

The “No-touch” technique improves the survival of patients with advanced hepatocellular carcinomas treated by liver transplantation: A single-center prospective randomized controlled trial

Background:Liver transplantation(LT)is the best treatment for patients with hepatocellular carcinoma(HCC).However,the surgical technique needs to be improved.The present study aimed to evaluate the“no-touch”technique in LT.Methods:From January 2018 to December 2019,we performed a prospective randomized controlled trial on HCC patients who underwent LT.The patients were randomized into two groups:a no-touch technique LT group(NT group,n=38)and a conventional LT technique group(CT group,n=46).Operative outcomes and survival in the two groups were analyzed.Results:The perioperative parameters were comparable between the two groups(P>0.05).There was no significant difference between the two groups in disease-free survival(DFS)(P=0.732)or overall survival(OS)(P=0.891).Of 36 patients who were beyond the Hangzhou criteria for LT,the DFS of the patients in the NT group was significantly longer than that in the CT group(median 402 vs.126 days,P=0.025).In 31 patients who had portal vein tumor thrombosis(PVTT),DFS and OS in the NT group were significantly better than those in the CT group(median DFS 420 vs.167 days,P=0.022;2-year OS rate 93.8%vs.66.7%,P=0.043).In 14 patients who had diffuse-type HCCs,DFS and OS were significantly better in the NT group than those in the CT group(median DFS 141 vs.56 days,P=0.008;2-year OS rate 75.0%vs.33.3%,P=0.034).Multivariate analysis showed that for patients with PVTT and diffusetype HCCs,the no-touch technique was an independent favorable factor for OS(PVTT:HR=0.018,95%CI:0.001-0.408,P=0.012;diffuse-type HCCs:HR=0.034,95%CI:0.002-0.634,P=0.024).Conclusions:The no-touch technique improved the survival of patients with advanced HCC compared with the conventional technique.The no-touch technique may provide a new and effective LT technique for advanced HCCs.

Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells:A systematic review

BACKGROUND Adipose-derived stem cells(ADSCs)and the stromal vascular fraction(SVF)have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions.Traditional enzymatic methods for isolating these cells face challenges such as high costs,lengthy processing time,and regulatory complexities.AIM This systematic review aimed to assess the efficacy and practicality of nonenzymatic,mechanical methods for isolating SVF and ADSCs,comparing these to conventional enzymatic approaches.METHODS Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,a comprehensive literature search was conducted across multiple databases.Studies were selected based on inclusion criteria focused on non-enzymatic isolation methods for SVF and ADSCs from adipose tissue.The risk of bias was assessed,and a qualitative synthesis of findings was performed due to the methodological heterogeneity of the included studies.RESULTS Nineteen studies met the inclusion criteria,highlighting various mechanical techniques such as centrifugation,vortexing,and ultrasonic cavitation.The review identified significant variability in cell yield and viability,and the integrity of isolated cells across different non-enzymatic methods compared to enzymatic procedures.Despite some advantages of mechanical methods,including reduced processing time and avoidance of enzymatic reagents,the evidence suggests a need for optimization to match the cell quality and therapeutic efficacy achievable with enzymatic isolation.CONCLUSION Non-enzymatic,mechanical methods offer a promising alternative to enzymatic isolation of SVF and ADSCs,potentially simplifying the isolation process and reducing regulatory hurdles.However,further research is necessary to standardize these techniques and ensure consistent,high-quality cell yields for clinical applications.The development of efficient,safe,and reproducible non-enzymatic isolation methods could significantly advance the field of regenerative medicine.

Reducing intimal hyperplasia in vein grafts harvested by a no-touch harvesting technique

Objective To investigate the effect of no-touch harvesting technique in reducing vein graft intimal hyperplasia. Methods This longitudinal trial compared graft angiostenosis of two groups undergoing jugular vein to carotid artery interposition grafting in rabbit model. Conventional group:12 rabbits had their veins stripped,distended,and stored in heparinized saline solution. No-touch group:12 rabbits had veins removed with surrounding tissues,but were not distended,and stored in heparinized blood. The grafts were removed 4 weeks following grafting,and morphometry and immunohistochemistry assessment were performed. Results The intimal thickness,degree of angiostenosis and proliferation index of vascular smooth muscle cells of no-touch group were significantly reduced (P<0.01) compared with those of the conventional group. The proliferating cell nuclear antigen positive-staining cells were significantly increased (P<0.01) in the conventional group compared with whose in the no-touch group. Conclusion Harvesting the vein graft with no-touch harvesting technique could significantly reduce intimal hyperplasia of the vein graft.

An effective technique for isolating adult activated Schwann cells

BACKGROUND： Schwann cells （SCs） are neuroglial cells of peripheral nerve and play a key role in repairing peripheral nerve injury; therefore, it provides an important evidence for transplantation of SCs which are characterized by active proliferation and adult high-purity in vitro after nerve injury in clinic, and also develops a new therapeutic way for nerve injury.OBJECTEVE： To investigate an effective technique for isolating adult activated Schwann cells,DESIGN： Controlled observational study.SETTING： Mudanjiang Medical College.MATERIALS： The experiment was completed at the Department of Medical Genetics of Harbin Medical University from March 2003 to April 2005. Health female Wistar rats, aged 2 months, weighting 150-160 g, were randomly divided into 3 groups with 5 in each group.METHODS： The right sciatic nerves from 15 Wistar rats were exposed and transected at the mid thigh under pentobarbital anesthesia （4 mg/kg, Lp）. Seven days later, the distal segments of the predegenerated nerves were removed and used to produce adult Schwann cell cultures. The distal segment of the predegenerated nerve, 20 mm in length, was resected. The nerve was cut into pieces 1 mm in length and incubated for 3 hours under CO2 at 37 ℃ with an enzyme mixture of 0.05% collagenase/dispase. Rats were divided into 3 groups： ① Group 1： The nerve fragments were explanted in poly-L-lysine and laminin-coated dishes with BS medium from the 1st to the 6th day, On the 6^th day, the fragments were removed into a new poly-L-lysine-laminin-coated dish and the BS medium was changed to BS with 10% FBS, The nerve fragments were replaced repeatedly in the same way in new dishes on the 12^th and the 18th days. ② Group 2： For the first 3 days, the nerve fragments were fed with BS with 10% FBS. This medium was changed to BS medium on the third day. The nerve fragments were removed to another dish on day 6 and BS medium was changed to BS with 25 mI.JL FBS. Hereafter the culture method was the same as for group 1. ③ Group 3： For the first 6 days, nerve fragments were incubated in a dish not coated with poly-L-lysine and laminin, in BS medium supplemented with 8×10^7 U/L of penicillin-streptomycin. On the 6th day, the nerve fragments were removed to a poly-L-lysine-laminin-coated dish and cultured in BS with 25 mI.JL FBS, On the 12th day, the nerve fragments were explanted a second dish and fed with BS containing 100 mL/L FBS. On the 18^th day, they were explanted to a third poly-L-lysine-laminin-coated dish, SCs were obtained from all 3 dishes on the 21st day, Finally, purity and density of SCs were identified and proliferation index was calculated at the same time.MAIN OUTCOME MEASURES ： Purity and density of SCs cultured with various methods in the three groups for 21 days.RESULTS ： ① Isolation and proliferation of SCs： In the group 1, they increased in number after 4 days and both purity and density of cultured SCs were significantly higher than those from group 2. In the group 2, there were few fibroblasts. In the group 3, both purity and density of cultured SCs were remarkably higher than in those from groups 1 or 2. Then optimal proliferation was soon seen and the rapid expansion of SC populations suppressed the development of contaminating fibroblasts. On the 21st day, SCs proliferated to achieve maximal density and were too crowded to be counted. With Chi-square test, the data of the purity and the density were analyzed from groups 1 to 3, the result indicated X^2=430.47, P 〈 0.05. ② Characterization and proliferation rate of SCs： Immunostaining for S100 protein was evident in the cell soma and the processes of all three groups in cultures of SCs. SCs in vitro demonstrated typical bior tri-polar morphology, had oval nuclei, and stained brightly for $100. The proliferation rate of SCs was assessed with double fluorescence staining for BrdU and S100 on the 21^st day of all three groups in cultures. About 40%-50% of the total SCs in the each group showed BrdU incorporation.CONCLUSION： The method is to use predegeneration in vivo, differential speed culture supplemented with the penicillin-streptomycin in low concentration, and changing of the concentration of FBS in the BS medium from 0 to 100 mL/L. This method allows remarkable suppression of fibroblast growth and attainment of SC proliferation and purity, in a short time, from adult nerves.

Isolation of protoplast from callus of Populus euphratica and H^+ fluxes across plasma membrane under NaCl stress

We used callus of Populus euphratica Olive to isolate protoplasts, and IT fluxes across plasma membrane were investigated. The concentration of enzymes for protoplast isolation, e.g. cellulase, pectolyase, macerozyme, hemicellulase, and sorbitol content, incubation time were systemically studied. High yield and viability of protoplast was achieved after 6-8 hours incubation of P. euphratica callus in enzyme solution containing 1.5% （w：v） cellulase R-10, 0.1% （w：v） pectolyase Y-23, 0.2% （w：v） macerozyme R-10, 0.05% （w：v） hemicellulase and 0.75M）.80 mol·L^-1 sorbitol. Non-invasively ion selective microelectrode technique was used to access proton fluxes in the absence and presence of NaCl （20 mmol.L-1）. Salt-induced transient net IT effiux was observed in the plasma membrane ofP. euphratica cells. The shift of IT flux response to NaC1 shock and the relevance to salt tolerance were discussed.

Alternative technique to save ischemic bowel segment in management of neonatal short bowel syndrome: A case report

BACKGROUND Congenital short bowel syndrome(SBS)associated with malrotation,gut volvulus and jejuno-ileal atresia is a very rare condition.It is a severe challenge for surgeons to preserve residual ischemic bowel segment in the management of short bowel syndrome,especially in neonates.CASE SUMMARY We report a newborn baby with gut malrotation associated with jejuno-ileal atresia,congenital SBS and jejunal volvulus.Hematemesis and abdominal distention were noted.At laparotomy,malrotation associated with jejuno-ileal atresia,congenital SBS and jenunal volvulus was confirmed.The total length of the small bowel was 63 cm with proximal jejunal bowel segment measuring 38 cm,including 18 cm necrotic segment below the Treitz’s ligament and 20 cm severe ischemic segment.The distal part of the small bowel was 25 cm in length and only about 0.8 cm in diameter.Ladd’s procedure,necrotic segment resection and end-to-back duodeno-ileal anastomosis were performed.The residual severe ischemic jejunum was preserved with single proximal stoma and distal end closure.Three months later,to restore the continuity of the isolated gut segment,end-to-end duodeno-jejunal and jejuno-ileal anastomosis was performed.The entire functional small bowel length increased to 80 cm.Intravenous fluid therapy and parenteral nutrition were discontinued on the 10th day postoperatively.Twelve months later,her body weight was 9.5 kg.CONCLUSION Isolation of severe ischemic bowel segment and staged anastomosis to restore the gut continuity for infants with SBS are safe and feasible.

Robust Fault Detection and Isolation Based on Finite-frequency H__/H_∞ Unknown Input Observers and Zonotopic Threshold Analysis

This work proposes a robust fault detection and isolation scheme for discrete-time systems subject to actuator faults,in which a bank of H_/H∞ fault detection unknown input observers(UIOs) and a zonotopic threshold analysis strategy are considered. In observer design, finite-frequency H_ index based on the generalized Kalman-Yakubovich-Popov lemma and H∞ technique are utilized to evaluate worst-case fault sensitivity and disturbance attenuation performance, respectively. The proposed H_/H∞ fault detection observers are designed to be insensitive to the corresponding actuator fault only, but sensitive to others.Then, to overcome the weakness of predefining threshold for FDI decision-making, this work proposes a zonotopic threshold analysis method to evaluate the generated residuals. The FDI decision-making relies on the evaluation with a dynamical zonotopic threshold. Finally, numerical simulations are provided to show the feasibility of the proposed scheme.

Separation, Identification, Isolation and Characterization of Degradation Product of Osimertinib Tablets by UPLC-QTOF-MS/MS and NMR: Evaluation of <i>In-Silico</i>Safety Assessment for Osimertinib (OSM) and Degradation Product (DP)

The present work encompasses identification and characterization of major degradation product (DP) of OSM observed in base hydrolytic stress study. The separation of DP was carried out on a non-polar stationary phase by using high-performance liquid chromatography system (HPLC). Using waters X-bridge (250 mm × 4.6 mm, 5 μm) C18 column with gradient elution program. For the characterization study, stress samples were subjected to HPLC and UPLC-QTOF-MS/MS and based on mass fragmentation pattern, plausible structure was deduced. Further, the DP was isolated using semi-prepara- tive liquid chromatography and concentrated the fractions using lyophilization. The isolated DP was subjected to extensive 1D (1H, 13C, and DEPT-135) and 2D (COSY, HSQC and HMBC) nuclear magnetic resonance (NMR) studies to authenticate the structure. The impurity was unambiguously named as N-(2-((2-(dimethylamino)ethyl)(methyl)amino)-4-metho-xy-5-((4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)-3-methoxypropanamide. Add- itionally, the In-Silico structure activity relation (QSAR) assessed through statistical based software’s DEREK NexusTM, and MultiCASE, Case UltraTM widely accepted and respected software’s for DP and OSM.

A Compact Self-Isolated MIMO Antenna System for 5G Mobile Terminals

A compact self-isolated Multi Input Multi Output (MIMO) antennaarray is presented for 5G mobile phone devices. The proposed antenna systemis operating at the 3.5 GHz band (3400–3600 MHz) and consists of eight antennaelements placed along two side edges of a mobile device, which meets the currenttrend requirements of full-screen smartphone devices. Each antenna element isdivided into two parts, a front part and back part. The front part consists of anI-shaped feeding line and a modified Hilbert fractal monopole antenna, whereasthe back part is an L-shaped element shorted to the system ground by a0.5 mm short stub. A desirable compactness can be obtained by utilizing the Hilbert space-filling property where the antenna element’s overall planar size printedon the side-edge frame is just (9.57 mm × 5.99 mm). The proposed MIMO antenna system has been simulated, analyzed, fabricated and tested. Based on the selfisolated property, good isolation (better than 15 dB) is attained without employingadditional decoupling elements and/or isolation techniques, which increases system complexity and reduces the antenna efficiency. The scattering parameters,antenna efficiencies, antenna gains, and antenna radiation characteristics areinvestigated to assess the proposed antenna performance. For evaluating the proposed antenna array system performance, the Envelope Correlation Coefficients(ECCs), Mean Effective Gains (MEGs) and channel capacity are calculated.Desirable antenna and MIMO performances are evaluated to confirm the suitability of the proposed MIMO antenna system for 5G mobile terminals.

超声引导下水隔离技术在胰腺肿物穿刺活检中的应用

目的:评价超声引导下水隔离技术在胰腺肿物穿刺活检中的应用价值。方法:回顾性选取2019年1月至2023年8月郑州大学第一附属医院收治,被胃肠组织遮挡、需要明确性质的32例胰腺肿物患者,均运用超声引导下水隔离技术辅助穿刺活检。记录患者临床数据及穿刺后并发症发生情况。结果:32例均充分暴露了安全穿刺路径,顺利完成穿刺操作,取材成功率为100%;术后均获得了明确病理学诊断,分别为腺癌24例、神经内分泌瘤4例、炎性组织2例、鳞状细胞癌1例、实性假乳头状瘤1例。穿刺术后1例患者出现腹痛,给予对症处理后未再出现;穿刺术中及术后均未出现出血、感染、胰瘘、胃肠组织损伤等严重并发症。结论:超声引导下水隔离技术辅助胰腺肿物穿刺活检安全、可行。

医护协同管理规范机器人辅助恶性肿瘤手术隔离技术操作标准的研究

目的探讨护士主导医护协同管理模式以规范机器人辅助恶性肿瘤手术隔离技术操作的具体方法。方法选择2021年8月—2023年7月在聊城市人民医院手术室实施达芬奇机器人操作的328例恶性肿瘤手术患者作为研究对象。将2021年8月—2022年7月的158例手术患者设为管理前组,将2022年8月—2023年7月的170例手术患者设为管理后组。管理后成立医护协同管理团队,团队成员通过加强隔离技术管理,制定机器人手术隔离技术操作关键点并组织实施。比较2组手术隔离技术并发症发生情况、管理前后医护人员隔离技术操作正确率及理论考核成绩。结果管理后组手术隔离技术并发症总发生率为3.53%,低于管理前组的13.92%,差异有统计学意义(P<0.05);管理后组医护隔离技术操作正确率为94.43%,高于管理前组的56.80%,差异有统计学意义(P<0.05);管理后组理论考核成绩为(96.75±3.25)分,高于管理前组的(75.25±15.35)分,差异有统计学意义(P<0.05)。结论以护士主导,医护协同管理模式强化机器人辅助恶性肿瘤手术隔离技术操作可降低相关并发症,提高手术质量与手术效果。

手术室护士对隔离技术的认知及实施态度的质性研究

目的了解手术室护士对隔离技术的认知及实施态度,为提高手术室护士隔离技术执行情况提供参考。方法对某三甲医院11名手术室护士进行访谈,收集资料并整理分析。结果(1)行为态度:手术室护士对隔离技术的积极态度、不了解隔离技术丰富内涵、缺乏隔离技术相关知识。(2)主观规范:自我感知压力增加、工作团队的影响。(3)知觉行为控制:对手术隔离技术认知能力不足、缺乏相关制度与流程、工作负荷过重、缺乏相关管理能力的培训。结论目前隔离技术规范不健全、管理未得到足够重视。为加强手术隔离技术的行为管理,建议强化专业知识,优化手术隔离技术操作流程,构建手术隔离技术管理体系。

氟化物磁光晶体的研究进展与应用

光通信技术发展迅速,激光平均功率不断提高,磁光隔离器是保证激光单向传输、维持光学系统稳定高效工作的基础,而磁光介质作为磁光隔离器的核心部件也因此得以快速发展。首先简要介绍了几种磁光材料以及当前广泛应用的石榴石磁光晶体的缺点、法拉第效应的原理和应用,随后系统分析了作为新一代磁光晶体的各类氟化物磁光晶体的优缺点,最后展望了CeF_(3)磁光晶体潜在的应用前景以及未来氟化物磁光晶体的发展趋势。

Micro-vibration suppression and compensation techniques for in-orbit satellite:A review

Micro-vibration is an important factor affecting the imaging quality and pointing accu-racy of the in-orbit satellites.To address the problem of micro-vibration compensation,a general summary for modeling,analysis,suppression,and compensation approach should be outlined.In this review,micro-vibration characteristics and its impacts on the payloads are firstly analyzed.Afterwards,methods for micro-vibration measurement are provided.In detail,the principles and practical applications of these methods are introduced.Then,advanced technologies for micro-vibration suppression are summarized from micro-vibration source attenuation,transfer path opti-mization and sensitive load isolation.Two approaches have been found to be effective for micro-vibration compensation.The one is the Line-of-Sight(LOS)stabilization assisted with Inertial Ref-erence Unit(IRU).The other is using image restoration technology to remove the blur caused by platform jitter.The compensation technique and research status of the two techniques are reviewed.This work will provide researchers with technical guidelines for micro-vibration suppression.

内镜医师内镜黏膜下剥离术教学的实践与思考

内镜黏膜下剥离术(endoscopic submucosal dissection,ESD)是目前消化道早癌的首选微创治疗方法,其操作难度大,是国内内镜教学培训的热点和难点。本研究以重庆医科大学附属第一医院青年医师和进修ESD操作的内镜医师为教学对象,建立了ESD规范化培训流程。培训周期共3月,教学内容包括理论培训、临床观摩、简易离体猪胃模型操作、真实患者实践操作,以及结业考核。通过问卷调查,100%的学员认为重庆医科大学附属第一医院内镜中心ESD教学培训对于掌握ESD技术是非常必要的,且能明显提高学员的ESD操作水平。因此,重庆医科大学附属第一医院内镜中心ESD规范化培训流程总体设置合理,为我国内镜医师的教学与培训提供一定的借鉴与参考。

减隔震技术在海上升压站电气设备中的应用前景

海上升压站中的电气设备可能存在抗震性能不足的风险,需要提高抗震韧性。介绍了变电站电气设备的主要震害及减隔震装置在陆上电力设施中的应用现状,并基于江苏海域某海上升压站结构的地震动力响应特征,探讨电气设备减隔震应用的可行性。通过合适的减隔震设计可提升电气设备抗震性能,对今后海上升压站电气设备的减隔震技术的应用提出了建议。

既有建筑减隔震技术与施工应用现状

减隔震技术在既有建筑抗震加固中具有明显的优势。从国家政策和规范、减隔震技术特点、国内外典型工程应用、减隔震技术施工等方面进行了系统阐述,展示了既有建筑对减隔震技术的实际需求和应用现状,分析了减隔震技术措施在既有建筑改造时的施工思路、安装重点及实例工程的施工流程,并对比了不同抗震加固方式的经济性,最后对既有建筑减隔震技术施工的研究重点进行了展望,旨在为既有建筑抗震加固中对减隔震技术的选择和施工应用提供参考。

隔震支座在舟曲县峰迭新区第二幼儿园项目中的应用及施工特点分析

随着城市建设的不断发展,幼儿园项目作为基础教育设施的一部分,其建设也面临着新的挑战。论文围绕舟曲县峰迭新区第二幼儿园建设项目展开研究,重点关注隔震支座在项目中的应用。该项目采用框架结构,工程特点主要表现在施工难点上,包括多工序、交叉作业、室外管网连接、场地有限等方面。论文将深入分析工程特点,探讨施工中的难点,并提出解决方案,以确保工程的高效顺利进行,为抗震建筑和类似工程提供有益的经验和教训。