Objective To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila(L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China. Methods Seventy-nine isolates of ...Objective To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila(L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China. Methods Seventy-nine isolates of L. pneumophila were collected from environmental and clinical sources, including cooling towers, hot springs, bath water, fountains, and patients, and identified with 16 S rRNA gene analysis and serum agglutination assay. The isolates were then typed by Sequence-Based Typing(SBT), and Genotyping of forty-two LP1 strains were analyzed by means of multiple-locus VNTR analysis with 8 loci(MLVA-8). All strains were further analyzed for two virulence genes: Legionella vir homologue(lvh) and repeats in structural toxin(rtx A). The intracellular growth ability of 33 selected isolates was determined by examining their interaction with J774 cells. Results All isolates were identified to L. pneumophila including 11 serogroups, among which the main serogroup were LP1, accounting for 54.43%. Thirty-three different sequence types(STs) from five main clonal groups and five singletons were identified, along with 8 different MLVA patterns. Both the lvh and rtx A loci were found in all 79 strains. Thirty isolates showed high intracellular growth ability in J774 cells. Conclusion L. pneumophila is a potential threat to public health, and effective control and prevention strategies are urgently needed.展开更多
BACKGROUND Diffuse large B-cell lymphoma(DLBCL)is a common non-Hodgkin's lymphoma.R-CHOP is a protocol for long-term chemotherapy for DLBCL patients.Longterm chemotherapy can lead to low immunity and increase the ...BACKGROUND Diffuse large B-cell lymphoma(DLBCL)is a common non-Hodgkin's lymphoma.R-CHOP is a protocol for long-term chemotherapy for DLBCL patients.Longterm chemotherapy can lead to low immunity and increase the risk of opportunistic pathogen infections in immunocompromised patients.CASE SUMMARY We report a case of coinfection with Pneumocystis jirovecii(P.jirovecii)and Legionella pneumophila(L.pneumophila)in a patient with DLBCL.The patient was a 40-year-old female who was diagnosed with DLBCL and was admitted due to pulmonary infection.P.jirovecii and L.pneumophila were detected in her bronchoalveolar lavage fluid by hexamine silver staining,isothermal amplification and metagenomic sequencing.CONCLUSION To the best of our knowledge,this is the first case of P.jirovecii and L.pneumophila coinfection found in a DLBCL patient.Clinicians should be aware of the risk of complicated infection in patients undergoing long-term chemotherapy.展开更多
Legionella pneumophila infection can cause Legionnaires’disease,a severe form of pneumonia.Extrapulmonary manifestations of Legionella infections include myocarditis,pericarditis,and endocarditis.We present a rare ca...Legionella pneumophila infection can cause Legionnaires’disease,a severe form of pneumonia.Extrapulmonary manifestations of Legionella infections include myocarditis,pericarditis,and endocarditis.We present a rare case of pneumonia caused by Legionella pneumophila with a possible etiologic link to a recently recovered culture-negative infective endocarditis.展开更多
Objective:To investigate the prevalence,isolation,identification,characterization,antibiotic profile and pathogenicity of Legionellae isolated from various set of waters.Methods:A total of 400 water samples were colle...Objective:To investigate the prevalence,isolation,identification,characterization,antibiotic profile and pathogenicity of Legionellae isolated from various set of waters.Methods:A total of 400 water samples were collected from different water sources.Water samples were pretreated using acid treatment followed by concentration and culture on buffered charcoal yeast extract agar.Parameters like ability of Legionella isolates to grow in various p H range,effect of different concentrations of chlorine and effect of different temperature optima were set up.Biochemical tests were performed to separate Legionellae into species.Antibiotic sensitivity tests and test for pathogenicity were also conducted on isolated strains.Results:The rates of isolation of Legionella pneumophila(L.pneumophila) in different water sources were found to be 20%(lakes),10%(ponds),8%(water-tanks) and 1%(rivers).Most of the isolates could grow in variable p H 6–8 and it could also survive the normal level of chlorination and even at temperature of 42 C.Isolated species of Legionellae resulted in identification of 5 different species,L.pneumophila being the dominant one.Strains of L.pneumophila were resistant to many antibiotics.Inoculation of Legionellae into intracerebral route of suckling mice revealed that L.pneumophila was the most virulent.Conclusions:Serious and fatal L.pneumophila infections may be transmitted through water.Legionella can survive under various conditions in various water sources.L.pneumophila is the important pathogen causing human disease.Great challenge prevails to health care professionals because these Legionellae acquired antibiotic resistance to many routinely prescribed antibiotics.展开更多
BACKGROUND Legionella pneumophila(L.pneumophila)is a gram-negative intracellular bacillus composed of sixteen different serogroups.It is mostly known to cause pneumonia in individuals with known risk factors as immuno...BACKGROUND Legionella pneumophila(L.pneumophila)is a gram-negative intracellular bacillus composed of sixteen different serogroups.It is mostly known to cause pneumonia in individuals with known risk factors as immunocompromised status,tobacco use,chronic organ failure or age older than 50 years.Although parapneumonic pleural effusion is frequent in legionellosis,pleural empyema is very uncommon.In this study,we report a case of fatal pleural empyema caused by L.pneumophila serogroup 1 in an 81-year-old man with multiple risk factors.CASE SUMMARY An 81-year-old man presented to the emergency with a 3 wk dyspnea,fever and left chest pain.His previous medical conditions were chronic lymphocytic leukemia,diabetes mellitus,chronic kidney failure,hypertension and hyperlipidemia,without tobacco use.Chest X-ray and comouted tomographyscan confirmed a large left pleural effusion,which puncture showed a citrine exudate with negative standard bacterial cultures.Despite intravenous cefotaxime antibiotherapy,patient’s worsening condition after 10 d led to thoracocentesis and evacuation of 2 liters of pus.The patient progressively developed severe hypoxemia and multiorgan failure occurred.The patient was treated by antibiotherapy with cefepime and amikacin and with adequate symptomatic shock treatment,but died of uncontrolled sepsis.The next day,cultures of the surgical pleural liquid samples yielded L.pneumophila serogroup 1,consistent with the diagnosis of pleural legionellosis.CONCLUSION L.pneumophila should be considered in patients with multiple risk factors and undiagnosed pleural empyema unresponsive to conventional antibiotherapy.展开更多
<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying...<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying and monitoring of <i>Legionella</i> in the environment using conventional and modern technologies. The present study aims to compare detection of <i>L. pneumophila</i> in water samples using both culture and PCR techniques. A pre-enriched contaminated water sample was split into 13 subsamples. Culture and PCR tests were done from the subsamples after different intervals. The results showed a positive PCR result for <i>L. pneumophila</i> after 8 h of incubation. Also, <i>L. pneumophila</i> was detected by culture on non-selective BCYNE agar and selective GPVC agar after 5 and 6 days of incubation respectively. There was no significant difference between the non-selective BCYE- and the selective GVPC method. The PCR procedure was found more sensitive and differed significantly from the conventional selective GVPC method in isolation of <i>L. pneumophila</i> from water samples. It was concluded that pre-enrichment incubation allows the detection of <i>L. pneumophila</i> by PCR within a maximum of 12 h from the collection of water samples.展开更多
目的观察组蛋白去乙酰化酶6(HDAC6)调控嗜肺军团菌鞭毛重组蛋白A(rflaA)对小鼠巨噬细胞自噬相关因子表达的影响,分析其可能的作用机制。方法采用支气管肺泡灌洗法提取C57BL/6J与HDAC6-/-C57BL/6J两种小鼠巨噬细胞,用小鼠巨噬细胞表面标...目的观察组蛋白去乙酰化酶6(HDAC6)调控嗜肺军团菌鞭毛重组蛋白A(rflaA)对小鼠巨噬细胞自噬相关因子表达的影响,分析其可能的作用机制。方法采用支气管肺泡灌洗法提取C57BL/6J与HDAC6-/-C57BL/6J两种小鼠巨噬细胞,用小鼠巨噬细胞表面标记物F4/80鉴定;纯化rflaA,CCK-8法检测其对小鼠巨噬细胞半数抑制浓度(IC50),筛选最佳作用浓度用于后续实验;rflaA分别干预C57BL/6J及HDAC6-/-C57BL/6J小鼠巨噬细胞6、12和24 h,RT-qPCR、Western blot及免疫荧光检测各组自噬相关因子自噬效应蛋白1(Beclin1)、自噬相关蛋白5(ATG5)、自噬微管相关蛋白轻链3(LC3)、SQSTM1蛋白(P62)的表达水平。结果根据CCK-8结果计算,IC50为0.41μg·μL^(-1),最佳作用浓度为0.041μg·μL^(-1)用于后续实验;RT-qPCR、Western blot、免疫荧光结果显示,rflaA干预C57BL/6J小鼠巨噬细胞后,与0 h相比,6、12、24 h HDAC6的表达水平升高,LC3、ATG5表达水平均降低,P62的表达水平升高,Beclin1的表达水平先降低后升高(P均<0.05);rflaA干预HDAC6-/-C57BL/6J小鼠巨噬细胞后,与0 h相比,6、12、24 h LC3、ATG5、Beclin1表达水平均升高,P62的表达水平降低(P均<0.05);在相同的时间点,与C57BL/6J小鼠巨噬细胞各组相比,HDAC6敲除后各组细胞ATG5、LC3表达水平升高,Beclin1先升高后降低,P62降低(P均<0.05)。结论HDAC6促进rflaA抑制小鼠巨噬细胞自噬,HDAC6可能通过影响微管蛋白乙酰化干扰巨噬细胞自噬。展开更多
基金supported by grants from the National Natural Science Foundation of China [81672048]the Youth Innovation Project of Sichuan Medical Association Foundation [Q15081]the Priority Project on Infectious Disease Control and Prevention [2018ZX10714002]
文摘Objective To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila(L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China. Methods Seventy-nine isolates of L. pneumophila were collected from environmental and clinical sources, including cooling towers, hot springs, bath water, fountains, and patients, and identified with 16 S rRNA gene analysis and serum agglutination assay. The isolates were then typed by Sequence-Based Typing(SBT), and Genotyping of forty-two LP1 strains were analyzed by means of multiple-locus VNTR analysis with 8 loci(MLVA-8). All strains were further analyzed for two virulence genes: Legionella vir homologue(lvh) and repeats in structural toxin(rtx A). The intracellular growth ability of 33 selected isolates was determined by examining their interaction with J774 cells. Results All isolates were identified to L. pneumophila including 11 serogroups, among which the main serogroup were LP1, accounting for 54.43%. Thirty-three different sequence types(STs) from five main clonal groups and five singletons were identified, along with 8 different MLVA patterns. Both the lvh and rtx A loci were found in all 79 strains. Thirty isolates showed high intracellular growth ability in J774 cells. Conclusion L. pneumophila is a potential threat to public health, and effective control and prevention strategies are urgently needed.
文摘BACKGROUND Diffuse large B-cell lymphoma(DLBCL)is a common non-Hodgkin's lymphoma.R-CHOP is a protocol for long-term chemotherapy for DLBCL patients.Longterm chemotherapy can lead to low immunity and increase the risk of opportunistic pathogen infections in immunocompromised patients.CASE SUMMARY We report a case of coinfection with Pneumocystis jirovecii(P.jirovecii)and Legionella pneumophila(L.pneumophila)in a patient with DLBCL.The patient was a 40-year-old female who was diagnosed with DLBCL and was admitted due to pulmonary infection.P.jirovecii and L.pneumophila were detected in her bronchoalveolar lavage fluid by hexamine silver staining,isothermal amplification and metagenomic sequencing.CONCLUSION To the best of our knowledge,this is the first case of P.jirovecii and L.pneumophila coinfection found in a DLBCL patient.Clinicians should be aware of the risk of complicated infection in patients undergoing long-term chemotherapy.
文摘Legionella pneumophila infection can cause Legionnaires’disease,a severe form of pneumonia.Extrapulmonary manifestations of Legionella infections include myocarditis,pericarditis,and endocarditis.We present a rare case of pneumonia caused by Legionella pneumophila with a possible etiologic link to a recently recovered culture-negative infective endocarditis.
基金Supported by Rockefeller Foundation,USA(Grant No.External-R.F-2396725281-2015-16)
文摘Objective:To investigate the prevalence,isolation,identification,characterization,antibiotic profile and pathogenicity of Legionellae isolated from various set of waters.Methods:A total of 400 water samples were collected from different water sources.Water samples were pretreated using acid treatment followed by concentration and culture on buffered charcoal yeast extract agar.Parameters like ability of Legionella isolates to grow in various p H range,effect of different concentrations of chlorine and effect of different temperature optima were set up.Biochemical tests were performed to separate Legionellae into species.Antibiotic sensitivity tests and test for pathogenicity were also conducted on isolated strains.Results:The rates of isolation of Legionella pneumophila(L.pneumophila) in different water sources were found to be 20%(lakes),10%(ponds),8%(water-tanks) and 1%(rivers).Most of the isolates could grow in variable p H 6–8 and it could also survive the normal level of chlorination and even at temperature of 42 C.Isolated species of Legionellae resulted in identification of 5 different species,L.pneumophila being the dominant one.Strains of L.pneumophila were resistant to many antibiotics.Inoculation of Legionellae into intracerebral route of suckling mice revealed that L.pneumophila was the most virulent.Conclusions:Serious and fatal L.pneumophila infections may be transmitted through water.Legionella can survive under various conditions in various water sources.L.pneumophila is the important pathogen causing human disease.Great challenge prevails to health care professionals because these Legionellae acquired antibiotic resistance to many routinely prescribed antibiotics.
文摘BACKGROUND Legionella pneumophila(L.pneumophila)is a gram-negative intracellular bacillus composed of sixteen different serogroups.It is mostly known to cause pneumonia in individuals with known risk factors as immunocompromised status,tobacco use,chronic organ failure or age older than 50 years.Although parapneumonic pleural effusion is frequent in legionellosis,pleural empyema is very uncommon.In this study,we report a case of fatal pleural empyema caused by L.pneumophila serogroup 1 in an 81-year-old man with multiple risk factors.CASE SUMMARY An 81-year-old man presented to the emergency with a 3 wk dyspnea,fever and left chest pain.His previous medical conditions were chronic lymphocytic leukemia,diabetes mellitus,chronic kidney failure,hypertension and hyperlipidemia,without tobacco use.Chest X-ray and comouted tomographyscan confirmed a large left pleural effusion,which puncture showed a citrine exudate with negative standard bacterial cultures.Despite intravenous cefotaxime antibiotherapy,patient’s worsening condition after 10 d led to thoracocentesis and evacuation of 2 liters of pus.The patient progressively developed severe hypoxemia and multiorgan failure occurred.The patient was treated by antibiotherapy with cefepime and amikacin and with adequate symptomatic shock treatment,but died of uncontrolled sepsis.The next day,cultures of the surgical pleural liquid samples yielded L.pneumophila serogroup 1,consistent with the diagnosis of pleural legionellosis.CONCLUSION L.pneumophila should be considered in patients with multiple risk factors and undiagnosed pleural empyema unresponsive to conventional antibiotherapy.
文摘<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying and monitoring of <i>Legionella</i> in the environment using conventional and modern technologies. The present study aims to compare detection of <i>L. pneumophila</i> in water samples using both culture and PCR techniques. A pre-enriched contaminated water sample was split into 13 subsamples. Culture and PCR tests were done from the subsamples after different intervals. The results showed a positive PCR result for <i>L. pneumophila</i> after 8 h of incubation. Also, <i>L. pneumophila</i> was detected by culture on non-selective BCYNE agar and selective GPVC agar after 5 and 6 days of incubation respectively. There was no significant difference between the non-selective BCYE- and the selective GVPC method. The PCR procedure was found more sensitive and differed significantly from the conventional selective GVPC method in isolation of <i>L. pneumophila</i> from water samples. It was concluded that pre-enrichment incubation allows the detection of <i>L. pneumophila</i> by PCR within a maximum of 12 h from the collection of water samples.
文摘目的观察组蛋白去乙酰化酶6(HDAC6)调控嗜肺军团菌鞭毛重组蛋白A(rflaA)对小鼠巨噬细胞自噬相关因子表达的影响,分析其可能的作用机制。方法采用支气管肺泡灌洗法提取C57BL/6J与HDAC6-/-C57BL/6J两种小鼠巨噬细胞,用小鼠巨噬细胞表面标记物F4/80鉴定;纯化rflaA,CCK-8法检测其对小鼠巨噬细胞半数抑制浓度(IC50),筛选最佳作用浓度用于后续实验;rflaA分别干预C57BL/6J及HDAC6-/-C57BL/6J小鼠巨噬细胞6、12和24 h,RT-qPCR、Western blot及免疫荧光检测各组自噬相关因子自噬效应蛋白1(Beclin1)、自噬相关蛋白5(ATG5)、自噬微管相关蛋白轻链3(LC3)、SQSTM1蛋白(P62)的表达水平。结果根据CCK-8结果计算,IC50为0.41μg·μL^(-1),最佳作用浓度为0.041μg·μL^(-1)用于后续实验;RT-qPCR、Western blot、免疫荧光结果显示,rflaA干预C57BL/6J小鼠巨噬细胞后,与0 h相比,6、12、24 h HDAC6的表达水平升高,LC3、ATG5表达水平均降低,P62的表达水平升高,Beclin1的表达水平先降低后升高(P均<0.05);rflaA干预HDAC6-/-C57BL/6J小鼠巨噬细胞后,与0 h相比,6、12、24 h LC3、ATG5、Beclin1表达水平均升高,P62的表达水平降低(P均<0.05);在相同的时间点,与C57BL/6J小鼠巨噬细胞各组相比,HDAC6敲除后各组细胞ATG5、LC3表达水平升高,Beclin1先升高后降低,P62降低(P均<0.05)。结论HDAC6促进rflaA抑制小鼠巨噬细胞自噬,HDAC6可能通过影响微管蛋白乙酰化干扰巨噬细胞自噬。