Litsea elliptica Blume has been traditionally used to treat headache,fever,and stomach ulcer,and has also been used as an insect repellent.The acute and subacute toxicities of L.elliptica essential oil were evaluated ...Litsea elliptica Blume has been traditionally used to treat headache,fever,and stomach ulcer,and has also been used as an insect repellent.The acute and subacute toxicities of L.elliptica essential oil were evaluated orally by gavage in female Sprague-Dawley rats.For the acute toxicity study,L.elliptica essential oil was administered in doses from 500 to 4 000 mg/kg(single dose),and in the subacute toxicity test,the following doses were used:125,250,and 500 mg/kg,for 28 consecutive days.In the acute toxicity study,L.elliptica essential oil caused dose-dependent adverse behaviours and mortality.The median lethal dose value was 3 488.86 mg/kg and the acute non-observed-adversed-effect level value was found to be 500 mg/kg.The subacute toxicity study of L.elliptica essential oil did not reveal alterations in body weight,and food and water consumptions.The haematological and biochemical analyses did not show significant differences between control and treated groups in most of the parameters examined,except for the hemoglobin,mean cell hemoglobin concentration,mean cell volume,mean cell hemoglobin,serum albumin,and serum sodium.However,these differences were still within the normal range.No abnormalities or histopathological changes were observed in the liver,pancreatic islet of Langerhans,and renal glomerulous and tubular cells of all treated groups.In conclusion,L.elliptica essential oil can be classified in the U group,which is defined as a group unlikely to present an acute hazard according to World Health Organization(WHO) classification.展开更多
目的评价蒲地蓝消炎糖浆对幼龄SD大鼠毒性研究。方法选用出生后第13日(postnatal day 13,PND13)的幼龄SD大鼠240只,分为空白对照组、辅料对照组和蒲地蓝消炎糖浆低、中、高剂量组(2、6、15 g生药/kg),连续灌胃13周,观察指标包括一般观...目的评价蒲地蓝消炎糖浆对幼龄SD大鼠毒性研究。方法选用出生后第13日(postnatal day 13,PND13)的幼龄SD大鼠240只,分为空白对照组、辅料对照组和蒲地蓝消炎糖浆低、中、高剂量组(2、6、15 g生药/kg),连续灌胃13周,观察指标包括一般观察、体重、摄食量、身体发育指标、反射指标、性征指标、血液学、凝血指标、血清生化、性激素、生长激素、胫骨长度、骨密度、脏器重量、脏器系数及组织病理学检查等。结果蒲地蓝消炎糖浆各剂量组未见明显药物相关毒性反应,恢复期结束后未见迟缓毒性发生。结论在本试验条件下,蒲地蓝消炎糖浆对幼龄大鼠未观察到损害作用剂量(NOAEL)为15 g生药/kg。展开更多
基金Project supported by the Science Fund,Ministry of Science,Technology and Innovation,Malaysia (No. 02-01-02-SF0205)the Universiti Kebangsaan Malaysia (No. UKM-OUP-TKP-21-101/2011)
文摘Litsea elliptica Blume has been traditionally used to treat headache,fever,and stomach ulcer,and has also been used as an insect repellent.The acute and subacute toxicities of L.elliptica essential oil were evaluated orally by gavage in female Sprague-Dawley rats.For the acute toxicity study,L.elliptica essential oil was administered in doses from 500 to 4 000 mg/kg(single dose),and in the subacute toxicity test,the following doses were used:125,250,and 500 mg/kg,for 28 consecutive days.In the acute toxicity study,L.elliptica essential oil caused dose-dependent adverse behaviours and mortality.The median lethal dose value was 3 488.86 mg/kg and the acute non-observed-adversed-effect level value was found to be 500 mg/kg.The subacute toxicity study of L.elliptica essential oil did not reveal alterations in body weight,and food and water consumptions.The haematological and biochemical analyses did not show significant differences between control and treated groups in most of the parameters examined,except for the hemoglobin,mean cell hemoglobin concentration,mean cell volume,mean cell hemoglobin,serum albumin,and serum sodium.However,these differences were still within the normal range.No abnormalities or histopathological changes were observed in the liver,pancreatic islet of Langerhans,and renal glomerulous and tubular cells of all treated groups.In conclusion,L.elliptica essential oil can be classified in the U group,which is defined as a group unlikely to present an acute hazard according to World Health Organization(WHO) classification.
文摘目的评价蒲地蓝消炎糖浆对幼龄SD大鼠毒性研究。方法选用出生后第13日(postnatal day 13,PND13)的幼龄SD大鼠240只,分为空白对照组、辅料对照组和蒲地蓝消炎糖浆低、中、高剂量组(2、6、15 g生药/kg),连续灌胃13周,观察指标包括一般观察、体重、摄食量、身体发育指标、反射指标、性征指标、血液学、凝血指标、血清生化、性激素、生长激素、胫骨长度、骨密度、脏器重量、脏器系数及组织病理学检查等。结果蒲地蓝消炎糖浆各剂量组未见明显药物相关毒性反应,恢复期结束后未见迟缓毒性发生。结论在本试验条件下,蒲地蓝消炎糖浆对幼龄大鼠未观察到损害作用剂量(NOAEL)为15 g生药/kg。
