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Active Fault Tolerant Control of a Class of Nonlinear Time-Delay Processes 被引量:8
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作者 王东 周东华 金以慧 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2004年第1期60-65,共6页
Based on a nonlinear state predictor (NSP) and a strong tracking filter (STF), a sensor fault tolerant generic model control (FTGMC) approach for a class of nonlinear time-delay processes is proposed. First, the NSP i... Based on a nonlinear state predictor (NSP) and a strong tracking filter (STF), a sensor fault tolerant generic model control (FTGMC) approach for a class of nonlinear time-delay processes is proposed. First, the NSP is introduced, and it is used to extend the conventional generic model control (GMC) to nonlinear processes with large input time-delay. Then the STF is adopted to estimate process states and sensor bias, the estimated sensor bias is used to drive a fault detection logic. When a sensor fault is detected, the estimated process states by the STF will be used to construct the process output to form a 'soft sensor', which is then used by the NSP (instead of the real outputs) to provide state predictors. These procedures constitute an active fault tolerant control scheme. Finally, simulation results of a three-tank-system demonstrate the effectiveness of the proposed approach. 展开更多
关键词 fault tolerant control TIME-DELAY nonlinear processes nonlinear state predictor strong tracking filter
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Improved Kernel PLS-based Fault Detection Approach for Nonlinear Chemical Processes 被引量:5
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作者 王丽 侍洪波 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2014年第6期657-663,共7页
In this paper, an improved nonlinear process fault detection method is proposed based on modified kernel partial least squares(KPLS). By integrating the statistical local approach(SLA) into the KPLS framework, two new... In this paper, an improved nonlinear process fault detection method is proposed based on modified kernel partial least squares(KPLS). By integrating the statistical local approach(SLA) into the KPLS framework, two new statistics are established to monitor changes in the underlying model. The new modeling strategy can avoid the Gaussian distribution assumption of KPLS. Besides, advantage of the proposed method is that the kernel latent variables can be obtained directly through the eigen value decomposition instead of the iterative calculation, which can improve the computing speed. The new method is applied to fault detection in the simulation benchmark of the Tennessee Eastman process. The simulation results show superiority on detection sensitivity and accuracy in comparison to KPLS monitoring. 展开更多
关键词 nonlinear process fault detection kernel partial least squares statistical local approach
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Remaining useful life prediction for a nonlinear multi-degradation system with public noise 被引量:6
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作者 ZHANG Hanwen CHEN Maoyin ZHOU Donghua 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第2期429-435,共7页
To predict the remaining useful life(RUL) for a class of nonlinear multi-degradation systems, a method is presented. In the real industrial processes, systems are usually composed by several parts or components, and t... To predict the remaining useful life(RUL) for a class of nonlinear multi-degradation systems, a method is presented. In the real industrial processes, systems are usually composed by several parts or components, and these parts or components are working in the same environment, thus the degradations of these parts or components will be influenced by common factors. To describe such a phenomenon in degradations, a multi-degradation model with public noise is proposed. To identify the degradation states and the unknown parameters, an iterative estimation method is proposed by using the Kalman filter and the expectation maximization(EM) algorithm. Next, with known thresholds,the RUL of each degradation can be predicted by using the first hitting time(FHT). In addition, the RUL of the whole system can be obtained by a Copula function. Finally, a practical case is used to demonstrate the method proposed. 展开更多
关键词 remaining useful life(RUL) multi-degradation system public noise nonlinear degradation process
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Three-Dimensional Structure of Optimal Nonlinear Excitation for Decadal Variability of the Thermohaline Circulation 被引量:2
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作者 ZU Zi-Qing MU Mu Henk A.DIJKSTRA 《Atmospheric and Oceanic Science Letters》 CSCD 2013年第6期410-416,共7页
The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results s... The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results show that the optimal initial perturbations of temperature and salinity exciting the strongest decadal THC variations have similar structures: the perturbations are mainly in the northwestern basin at a depth ranging from 1500 to 3000 m. These temperature and salinity perturbations act as the optimal precursors for future modifications of the THC, highlighting the importance of observations in the northwestern basin to monitor the variations of temperature and salinity at depth. The decadal THC variation in the nonlinear model initialized by the optimal salinity perturbations is much stronger than that caused by the optimal temperature perturbations, indicating that salinity variations might play a relatively important role in exciting the decadal THC variability. Moreover, the decadal THC variations in the tangent linear and nonlinear models show remarkably different characteristics, suggesting the importance of nonlinear processes in the decadal variability of the THC. 展开更多
关键词 thermohaline circulation decadal variability conditional nonlinear optimal perturbation optimal precursor nonlinear processes
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Real time remaining useful life prediction based on nonlinear Wiener based degradation processes with measurement errors 被引量:22
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作者 唐圣金 郭晓松 +3 位作者 于传强 周志杰 周召发 张邦成 《Journal of Central South University》 SCIE EI CAS 2014年第12期4509-4517,共9页
Real time remaining useful life(RUL) prediction based on condition monitoring is an essential part in condition based maintenance(CBM). In the current methods about the real time RUL prediction of the nonlinear degrad... Real time remaining useful life(RUL) prediction based on condition monitoring is an essential part in condition based maintenance(CBM). In the current methods about the real time RUL prediction of the nonlinear degradation process, the measurement error is not considered and forecasting uncertainty is large. Therefore, an approximate analytical RUL distribution in a closed-form of a nonlinear Wiener based degradation process with measurement errors was proposed. The maximum likelihood estimation approach was used to estimate the unknown fixed parameters in the proposed model. When the newly observed data are available, the random parameter is updated by the Bayesian method to make the estimation adapt to the item's individual characteristic and reduce the uncertainty of the estimation. The simulation results show that considering measurement errors in the degradation process can significantly improve the accuracy of real time RUL prediction. 展开更多
关键词 remaining useful life Wiener based degradation process measurement error nonlinear maximum likelihood estimation Bayesian method
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Higher-Order Statistics and Nonlinear Processes in Space Plasmas
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作者 Zhao Zhengyu Dai Honggang Shi Xianqing 《Wuhan University Journal of Natural Sciences》 EI CAS 1998年第2期181-186,共6页
Statistics of order 2 (variance, auto and cross-correlation functions, auto and cross-power spectra) and 3 (skewness, auto and cross-bicorrelation functions, auto and cross-bispectra) are used to analyze the wave-part... Statistics of order 2 (variance, auto and cross-correlation functions, auto and cross-power spectra) and 3 (skewness, auto and cross-bicorrelation functions, auto and cross-bispectra) are used to analyze the wave-particle interaction in space plasmas. The signals considered here are medium scale electron density irregularities and ELF/ULF electrostatic turbulence. Nonlinearities are mainly observed in the ELF range. They are independently pointed out in time series associated with fluctuations in electronic density and in time series associated with the measurement of one electric field component. Peaks in cross-bicorrelation function and in mutual information clearly show that, in well delimited frequency bands, the wave-particle interactions are nonlinear above a certain level of fluctuations. The way the energy is transferred within the frequencies of density fluctuations is indicated by a bi-spectra analysis. 展开更多
关键词 higher-order statistics nonlinear processes space plasmas
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New Algorithm Model for Processing GeneralizedDynamic Nonlinear Data Derived from Deformation Monitoring Network
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作者 LINXiangguo LIANGYong 《Geo-Spatial Information Science》 2005年第2期133-137,共5页
The processing of nonlinear data was one of hot topics in surveying and mapping field in recent years. As a result, many linear methods and nonlinear methods have been developed. But the methods for processing general... The processing of nonlinear data was one of hot topics in surveying and mapping field in recent years. As a result, many linear methods and nonlinear methods have been developed. But the methods for processing generalized nonlinear surveying and mapping data, especially for different data types and including unknown parameters with random or nonrandom, are seldom noticed. A new algorithm model is presented in this paper for processing nonlinear dynamic multiple-period and multiple-accuracy data derived from deformation monitoring network. 展开更多
关键词 deformation monitoring generalized nonlinear data processing Marquardtmethod parameter estimate
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Effect of Nonlinear Dynamic Process on Formation and Breakdown of Blocking
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作者 张佩 倪允琪 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 1991年第1期41-50,共10页
With the L-P approximate method(variation of parameter method), a barotropic channel model in β-plane is used to study the effect of nonlinear interaction between two waves with different scales on the formation of b... With the L-P approximate method(variation of parameter method), a barotropic channel model in β-plane is used to study the effect of nonlinear interaction between two waves with different scales on the formation of blocking. The approximate analytical solution, which can describe the process of the blocking formation, maintenance and breakdown, has been obtained by using the method of aproximate expansion. The importance of nonlinear interaction between two waves with different scales is stressed in the solution. The result suggests that the nonlinear interaction is the main dynamic process of the blocking formation. Some required conditions of blocking formation are also discussed. 展开更多
关键词 PRO Effect of nonlinear Dynamic Process on Formation and Breakdown of Blocking
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On-Line Batch Process Monitoring Using Multiway Kernel Partial Least Squares 被引量:4
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作者 胡益 马贺贺 侍洪波 《Journal of Donghua University(English Edition)》 EI CAS 2011年第6期585-590,共6页
An approach for batch processes monitoring and fault detection based on multiway kernel partial least squares(MKPLS) was presented.It is known that conventional batch process monitoring methods,such as multiway partia... An approach for batch processes monitoring and fault detection based on multiway kernel partial least squares(MKPLS) was presented.It is known that conventional batch process monitoring methods,such as multiway partial least squares(MPLS),are not suitable due to their intrinsic linearity when the variations are nonlinear.To address this issue,kernel partial least squares(KPLS) was used to capture the nonlinear relationship between the latent structures and predictive variables.In addition,KPLS requires only linear algebra and does not involve any nonlinear optimization.In this paper,the application of KPLS was extended to on-line monitoring of batch processes.The proposed batch monitoring method was applied to a simulation benchmark of fed-batch penicillin fermentation process.And the results demonstrate the superior monitoring performance of MKPLS in comparison to MPLS monitoring. 展开更多
关键词 process monitoring fault detection kernel partial least squares(KPLS) nonlinear process multiway kernel partial least squares(MKPLS)
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Identification of nonlinear process described by neural fuzzy Hammerstein-Wiener model using multi-signal processing 被引量:1
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作者 Feng Li Li Jia Ya Gu 《Advances in Manufacturing》 SCIE EI CAS CSCD 2023年第4期694-707,共14页
In this study,a novel approach for nonlinear process identification via neural fuzzy-based Hammerstein-Wiener model with process disturbance by means of multi-signal processing is presented.The Hammerstein-Wiener mode... In this study,a novel approach for nonlinear process identification via neural fuzzy-based Hammerstein-Wiener model with process disturbance by means of multi-signal processing is presented.The Hammerstein-Wiener model consists of three blocks where a dynamic linear block is sandwiched between two static nonlinear blocks.Multi-signal sources are designed for achieving identification separation of the Hammerstein-Wiener process.The correlation analysis theory is utilized for estimating unknown parameters of output nonlinearity and linear block using separable signals,thus the interference of process disturbance is solved.Furthermore,the immeasurable intermediate variable and immeasurable noise term in identification model is taken over by auxiliary model output and estimate residuals,and then auxiliary model-based recursive extended least squares parameter estimation algorithm is derived to calculate parameters of the input nonlinearity and noise model.Finally,convergence analysis of the suggested identification scheme is derived using stochastic process theory.The simulation results indicate that proposed identification approach yields high identification accuracy and has good robustness. 展开更多
关键词 nonlinear process Parameter identification Hammerstein-Wiener model Neural fuzzy model Multiple signal processing
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A universal robust limit theorem for nonlinear Lévy processes under sublinear expectation
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作者 Mingshang Hu Lianzi Jiang +1 位作者 Gechun Liang Shige Peng 《Probability, Uncertainty and Quantitative Risk》 2023年第1期1-32,共32页
This article establishes a universal robust limit theorem under a sublinear expectation framework.Under moment and consistency conditions,we show that,forα∈(1,2),the i.i.d.sequence{(1/√∑_(i=1)^(n)X_(i),1/n∑_(i=1)... This article establishes a universal robust limit theorem under a sublinear expectation framework.Under moment and consistency conditions,we show that,forα∈(1,2),the i.i.d.sequence{(1/√∑_(i=1)^(n)X_(i),1/n∑_(i=1)^(n)X_(i)Y_(i),1/α√n∑_(i=1)^(n)X_(i))}_(n=1)^(∞)converges in distribution to L_(1),where L_(t=(ε_(t),η_(t),ζ_(t))),t∈[0,1],is a multidimensional nonlinear Lévy process with an uncertainty■set as a set of Lévy triplets.This nonlinear Lévy process is characterized by a fully nonlinear and possibly degenerate partial integro-differential equation(PIDE){δ_(t)u(t,x,y,z)-sup_(F_(μ),q,Q)∈■{∫_(R^(d)δλu(t,x,y,z)(dλ)with.To construct the limit process,we develop a novel weak convergence approach based on the notions of tightness and weak compactness on a sublinear expectation space.We further prove a new type of Lévy-Khintchine representation formula to characterize.As a byproduct,we also provide a probabilistic approach to prove the existence of the above fully nonlinear degenerate PIDE. 展开更多
关键词 Universal robust limit theorem Partial integro-differential equation nonlinear Lévy process α-stable distribution Sublinear expectation
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Infrared upconversion imaging in nonlinear metasurfaces 被引量:11
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作者 Rocio Camacho-Morales Davide Rocco +15 位作者 Lei Xu Valerio Flavio Gili Nikolay Dimitrov Lyubomir Stoyanov Zhonghua Ma Andrei Komar Mykhaylo Lysevych Fouad Karouta Alexander Dreischuh Hark Hoe Tan Giuseppe Leo Costantino De Angelis Chennupati Jagadish Andrey E.Miroshnichenko Mohsen Rahmani Dragomir N.Neshev 《Advanced Photonics》 EI CSCD 2021年第3期83-92,共10页
Infrared imaging is a crucial technique in a multitude of applications,including night vision,autonomous vehicle navigation,optical tomography,and food quality control.Conventional infrared imaging technologies,howeve... Infrared imaging is a crucial technique in a multitude of applications,including night vision,autonomous vehicle navigation,optical tomography,and food quality control.Conventional infrared imaging technologies,however,require the use of materials such as narrow bandgap semiconductors,which are sensitive to thermal noise and often require cryogenic cooling.We demonstrate a compact all-optical alternative to perform infrared imaging in a metasurface composed of GaAs semiconductor nanoantennas,using a nonlinear wave-mixing process.We experimentally show the upconversion of short-wave infrared wavelengths via the coherent parametric process of sum-frequency generation.In this process,an infrared image of a target is mixed inside the metasurface with a strong pump beam,translating the image from the infrared to the visible in a nanoscale ultrathin imaging device.Our results open up new opportunities for the development of compact infrared imaging devices with applications in infrared vision and life sciences. 展开更多
关键词 metasurfaces nonlinear optical processes infrared photonics imaging.
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Nonlinear Branching Processes with Immigration 被引量:1
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作者 Pei-Sen LI 《Acta Mathematica Sinica,English Series》 SCIE CSCD 2017年第8期1021-1038,共18页
The nonlinear branching process with immigration is constructed as the pathwise unique solution of a stochastic integral equation driven by Poisson random measures. Some criteria for the regularity, recurrence, ergodi... The nonlinear branching process with immigration is constructed as the pathwise unique solution of a stochastic integral equation driven by Poisson random measures. Some criteria for the regularity, recurrence, ergodicity and strong ergodicity of the process are then established. 展开更多
关键词 nonlinear branching process IMMIGRATION stochastic integral equation REGULARITY RECURRENCE ERGODICITY strong ergodicity
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Sensor Fault Tolerant Generic Model Control for Nonlinear Systems 被引量:1
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作者 谢晓清 周东华 金以慧 《Tsinghua Science and Technology》 EI CAS 2000年第2期201-207,共7页
A modified Strong Tracking Filter (STF) is used to develop a new approach to sensor fault tolerant control. Generic Model Control (GMC) is used to control the nonlinear process while the process runs normally becaus... A modified Strong Tracking Filter (STF) is used to develop a new approach to sensor fault tolerant control. Generic Model Control (GMC) is used to control the nonlinear process while the process runs normally because of its robust control performance. If a fault occurs in the sensor, a sensor bias vector is then introduced to the output equation of the process model. The sensor bias vector is estimated on line during every control period using the STF. The estimated sensor bias vector is used to develop a fault detection mechanism to supervise the sensors. When a sensor fault occurs, the conventional GMC is switched to a fault tolerant control scheme, which is, in essence, a state estimation and output prediction based GMC. The laboratory experimental results on a three tank system demonstrate the effectiveness of the proposed Sensor Fault Tolerant Generic Model Control (SFTGMC) approach. 展开更多
关键词 Generic Model Control (GMC) Strong Tracking Filter (STF) nonlinear processes fault tolerant control
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Operator-based Nonlinear Temperature Control Experiment for Microreactor Group Actuated by Peltier Devices
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作者 Shotaro Kawahata Ming-Cong Deng 《International Journal of Automation and computing》 EI CSCD 2016年第4期401-408,共8页
In this paper, operator-based nonlinear water temperature control for a group of three connected microreactors actuated by Peltier devices is proposed. To control the water temperature of tube in the microreactor, the... In this paper, operator-based nonlinear water temperature control for a group of three connected microreactors actuated by Peltier devices is proposed. To control the water temperature of tube in the microreactor, the temperature change of aluminum effects is considered. Therefore, the temperature change of aluminum becomes the part of an input of the tube. First, nonlinear thermal models of aluminum plates and tubes that structure the microreactor are obtained. Then, an operator based nonlinear water temperature control system for the microreactor is designed. Finally, the effectiveness of the proposed models and methods is confirmed by simulation and experimental results. 展开更多
关键词 Peltier device MICROREACTOR nonlinear control nonlinear process modeling OPERATOR continuous-time systems.
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Importance-Weighted Transfer Learning for Fault Classification under Covariate Shift
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作者 Yi Pan Lei Xie Hongye Su 《Intelligent Automation & Soft Computing》 2024年第4期683-696,共14页
In the process of fault detection and classification,the operation mode usually drifts over time,which brings great challenges to the algorithms.Because traditional machine learning based fault classification cannot d... In the process of fault detection and classification,the operation mode usually drifts over time,which brings great challenges to the algorithms.Because traditional machine learning based fault classification cannot dynamically update the trained model according to the probability distribution of the testing dataset,the accuracy of these traditional methods usually drops significantly in the case of covariate shift.In this paper,an importance-weighted transfer learning method is proposed for fault classification in the nonlinear multi-mode industrial process.It effectively alters the drift between the training and testing dataset.Firstly,the mutual information method is utilized to perform feature selection on the original data,and a number of characteristic parameters associated with fault classification are selected according to their mutual information.Then,the importance-weighted least-squares probabilistic classifier(IWLSPC)is utilized for binary fault detection and multi-fault classification in covariate shift.Finally,the Tennessee Eastman(TE)benchmark is carried out to confirm the effectiveness of the proposed method.The experimental result shows that the covariate shift adaptation based on importance-weight sampling is superior to the traditional machine learning fault classification algorithms.Moreover,IWLSPC can not only be used for binary fault classification,but also can be applied to the multi-classification target in the process of fault diagnosis. 展开更多
关键词 Covariate shift adaption nonlinear multi-mode process importance weight sampling multi-fault classification
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Sensor Network Design for Nonlinear Processes
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作者 李博 陈丙珍 《Tsinghua Science and Technology》 SCIE EI CAS 2003年第6期738-743,共6页
This paper presents a method to design a cost-optimal nonredundant sensor network to observe all variables in a general nonlinear process. A mixed integer linear programming model was used to minimize the cost with d... This paper presents a method to design a cost-optimal nonredundant sensor network to observe all variables in a general nonlinear process. A mixed integer linear programming model was used to minimize the cost with data classification to check the observability of all unmeasured variables. This work is a starting point for designing sensor networks for general nonlinear processes based on various criteria, such as reliability and accuracy. 展开更多
关键词 sensor network design nonlinear processes data classification
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The roles of heterogeneous chemical processes in the formation of an air pollution complex and gray haze 被引量:25
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作者 ZHU Tong,SHANG Jing & ZHAO DeFeng State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China 《Science China Chemistry》 SCIE EI CAS 2011年第1期145-153,共9页
Urban and regional air pollutions are characterized by high concentrations of secondary pollutants such as photo-oxidants (mainly ozone) and fine particulate matter, which are formed through chemical reactions of th... Urban and regional air pollutions are characterized by high concentrations of secondary pollutants such as photo-oxidants (mainly ozone) and fine particulate matter, which are formed through chemical reactions of the primary pollutants emitted from various sources. The accumulation of these pollutants under stagnant meteorological conditions results in the formation of gray haze, reducing visibility and causing major impacts on human health and climate. In an air pollution complex, the co- existence of high concentrations of primary and secondary gaseous and particulate pollutants provides a large amount of reac- tants for heterogeneous reactions on the surface of fine particles; these reactions change the oxidizing capacity of the atmos- phere, as well as chemical compositions along with the physicochemical and optical properties of particulate matter, thereby accelerating formation of the air pollution complex and gray haze. Using in situ technologies, such as diffuse reflectance infra- red Fourier-transform spectroscopy and single-particle Raman spectroscopy, we systematically investigated the reaction kinet- ics and mechanisms of gaseous pollutants (i.e., NO2, SO2, 03, and formaldehyde) on the surfaces of the major components of atmospheric particles such as CaCO3, kaolinite, montmorillonite, NaC1, sea salt, A1203, and Tit2. We found that the main re- action products were sulfate, nitrate, or formate, which can change the hygroscopicity and light extinction parameters of those particles significantly. By analyzing the reaction kinetics of these heterogeneous reactions, we identified synergetic mechanisms of the three ternary reaction systems, ,i.e., NOE-particles-H2O, SO2-particles-O3, and organics/SO2-particles-UV illumination. These synergetic mechanisms can provide experimental and theoretical bases for understanding the feedback mechanisms and nonlinear processes in the formation of an air pollution complex and gray haze. 展开更多
关键词 fine particles secondary pollutants oxidizing capacity ternary reaction nonlinear processes
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Optical vortex copier and regenerator in the Fourier domain 被引量:7
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作者 XIAODONG QIU FANGSHU LI +2 位作者 HAIGANG LIU XIANFENG CHEN LIXIANG CHEN 《Photonics Research》 SCIE EI 2018年第6期641-646,共6页
The generation and manipulation of optical vortices are of fundamental importance in a variety of promising applications. Here, we develop a nonlinear optical paradigm to implement self- and cross-convolution of optic... The generation and manipulation of optical vortices are of fundamental importance in a variety of promising applications. Here, we develop a nonlinear optical paradigm to implement self- and cross-convolution of optical vortex arrays, demonstrating the features of a vortex copier and regenerator. We use a phase-only spatial light modulator to prepare the 1064 nm invisible fundamental light to carry special optical vortex arrays and use a potassium titanyl phosphate crystal to perform type Ⅱ second-harmonic generation in the Fourier domain to achieve 532 nm visible structured vortices. Based on pure cross-convolution, we succeed in copying arbitrary-order single vortices as well as their superposition states onto a prearranged array of fundamental Gaussian spots. Also, based on the simultaneous effect of self- and cross-convolutions, we can expand the initial vortex lattices to regenerate more vortices carrying various higher topological charges. Our presented method of realizing imaging, an optical vortex copier and regenerator could find direct applications in optical mani optical communication, and quantum information processing with structured vortex pulation, optical arrays. 展开更多
关键词 Optical vortices Harmonic generation and mixing nonlinear optical signal processing
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Two-color multiphoton in vivo imaging with a femtosecond diamond Raman laser 被引量:1
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作者 Evan P Perillo Jeremy W Jarrett +7 位作者 Yen-Liang Liu Ahmed Hassan Daniel C Fernée John R Goldak Andrei Bonteanu David J Spence Hsin-Chih Yeh Andrew K Dunn 《Light(Science & Applications)》 SCIE EI CAS CSCD 2017年第1期400-407,共8页
Two-color multiphoton microscopy through wavelength mixing of synchronized lasers has been shown to increase the spectral window of excitable fluorophores without the need for wavelength tuning.However,most currently ... Two-color multiphoton microscopy through wavelength mixing of synchronized lasers has been shown to increase the spectral window of excitable fluorophores without the need for wavelength tuning.However,most currently available dual output laser sources rely on the costly and complicated optical parametric generation approach.In this report,we detail a relatively simple and low cost diamond Raman laser pumped by a ytterbium fiber amplifier emitting at 1055 nm,which generates a first Stokes emission centered at 1240 nm with a pulse width of 100 fs.The two excitation wavelengths of 1055 and 1240 nm,along with the effective two-color excitation wavelength of 1140 nm,provide an almost complete coverage of fluorophores excitable within the range of 1000–1300 nm.When compared with 1055 nm excitation,two-color excitation at 1140 nm offers a 90%increase in signal for many far-red emitting fluorescent proteins(for example,tdKatushka2).We demonstrate multicolor imaging of tdKatushka2 and Hoechst 33342 via simultaneous two-color two-photon,and two-color three-photon microscopy in engineered 3D multicellular spheroids.We further discuss potential benefits and applications for two-color three-photon excitation.In addition,we show that this laser system is capable of in vivo imaging in mouse cortex to nearly 1 mm in depth with two-color excitation. 展开更多
关键词 MICROSCOPY nonlinear processes three-dimensional imaging ultrafast lasers
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