Reciprocal translocation is a chromosomal structural abnormal- ity that arises when two non-homologous chromosomes rearrange and attach with each other, an incidence that occurs in about 1/500 to 1/625 newborns （Mack...Reciprocal translocation is a chromosomal structural abnormal- ity that arises when two non-homologous chromosomes rearrange and attach with each other, an incidence that occurs in about 1/500 to 1/625 newborns （Mackie and Scriven, 2002）. This event typically does not lead to any significant loss of genetic material, thus recip- rocal translocation carriers do not exhibit any severe abnormal phenotypes （Scriven et al., 1998; Zhang et al., 2016）.展开更多
Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identi...Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identified. Results show that somatic embryos of K. bipinnata var. integrifoliola include normal embryos, embryos with abnormal cotyledons, vitrified embryos, albino embryos, secondary embryos, linked embryos, embryos with abnormal growing points and embryos with expanding hypocotyl. After 40 d of callus culture, the response of normal somatic embryos from K. bipinnata var. integrifoliola was 26.7%, embryos with abnormal cotyledon 30.3% while other types of somatic embryos were below the 20% level. Most of normal embryos developed into plantlets and plantlet formation reached 94.9%. But the percentage of plantlet formation decreased apparently in abnormal embryos： the number of embryos with abnormal cotyledon declined to 76.1%, that of linked embryos to 47.4% and other types of abnormal embryos to below the 20% level. Albino embryos and embryos with abnormal growing point did not develop at all into plantlets. Embryos with abnormal cotyledons, linked embryos and embryos with abnormal growing points were observed during early stages of somatic embryogenesis, but vitrified, secondary and albino embryos and calli of embryos were observed at later stages. Increasing sucrose concentrations can decrease the occurrence of vitrified embryos, but the number of albino embryos decreased with an in- creasing in sucrose concentration.展开更多
以广东温氏集团培育的黄羽肉鸡种蛋胚胎为研究对象,并以与人和小鼠胚胎发育相关的胰岛素样生长因子2受体(insulin-like growth factor type II receptor,IGF2R)基因为候选基因,进行了SNP位点的筛选和多态性的检测,探讨了IGF2R的变异对...以广东温氏集团培育的黄羽肉鸡种蛋胚胎为研究对象,并以与人和小鼠胚胎发育相关的胰岛素样生长因子2受体(insulin-like growth factor type II receptor,IGF2R)基因为候选基因,进行了SNP位点的筛选和多态性的检测,探讨了IGF2R的变异对胚胎死亡的影响。在IGF2R第34外显子中发现2个SNP位点,对具有AlwNⅠ酶切位点的SNP位点进行了PCR-RFLP检测,分析了胚胎IGF2R的SNP位点PCR-RFLPs基因型在死亡和正常发育胚胎中分布特征。经2χ检验,鸡基因IGF2R第34外显子AlwNⅠ-RFLP基因型在死亡和正常发育胚胎中存在显著差异,在死亡的胚胎中基因型AT的频率(0.326)显著地高于正常发育胚胎的频率(0.077)。展开更多
基金supported by the National Natural Science Foundation of China (No. 31522034)Ministry of Science and Technology of China (2016YFC0900103)the National High Technology Research and Development Program Grant (2015AA020407)
文摘Reciprocal translocation is a chromosomal structural abnormal- ity that arises when two non-homologous chromosomes rearrange and attach with each other, an incidence that occurs in about 1/500 to 1/625 newborns （Mackie and Scriven, 2002）. This event typically does not lead to any significant loss of genetic material, thus recip- rocal translocation carriers do not exhibit any severe abnormal phenotypes （Scriven et al., 1998; Zhang et al., 2016）.
基金supported by the Foundation for Science and Technology Research and Development Program of Hebei Province (09225511)the Project of the Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, Beijing Forestry University, China (05– 04)
文摘Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identified. Results show that somatic embryos of K. bipinnata var. integrifoliola include normal embryos, embryos with abnormal cotyledons, vitrified embryos, albino embryos, secondary embryos, linked embryos, embryos with abnormal growing points and embryos with expanding hypocotyl. After 40 d of callus culture, the response of normal somatic embryos from K. bipinnata var. integrifoliola was 26.7%, embryos with abnormal cotyledon 30.3% while other types of somatic embryos were below the 20% level. Most of normal embryos developed into plantlets and plantlet formation reached 94.9%. But the percentage of plantlet formation decreased apparently in abnormal embryos： the number of embryos with abnormal cotyledon declined to 76.1%, that of linked embryos to 47.4% and other types of abnormal embryos to below the 20% level. Albino embryos and embryos with abnormal growing point did not develop at all into plantlets. Embryos with abnormal cotyledons, linked embryos and embryos with abnormal growing points were observed during early stages of somatic embryogenesis, but vitrified, secondary and albino embryos and calli of embryos were observed at later stages. Increasing sucrose concentrations can decrease the occurrence of vitrified embryos, but the number of albino embryos decreased with an in- creasing in sucrose concentration.
文摘以广东温氏集团培育的黄羽肉鸡种蛋胚胎为研究对象,并以与人和小鼠胚胎发育相关的胰岛素样生长因子2受体(insulin-like growth factor type II receptor,IGF2R)基因为候选基因,进行了SNP位点的筛选和多态性的检测,探讨了IGF2R的变异对胚胎死亡的影响。在IGF2R第34外显子中发现2个SNP位点,对具有AlwNⅠ酶切位点的SNP位点进行了PCR-RFLP检测,分析了胚胎IGF2R的SNP位点PCR-RFLPs基因型在死亡和正常发育胚胎中分布特征。经2χ检验,鸡基因IGF2R第34外显子AlwNⅠ-RFLP基因型在死亡和正常发育胚胎中存在显著差异,在死亡的胚胎中基因型AT的频率(0.326)显著地高于正常发育胚胎的频率(0.077)。
