Development of a new cerebral ischemia reperfusion model of Mongolian gerbils and standardized evaluation system

Background:The Mongolian gerbil is an excellent laboratory animal for preparing the cerebral ischemia model due to its inherent deficiency in the circle of Willis.However,the low incidence and unpredictability of symptoms are caused by numerous complex variant types of the circle.Additionally,the lack of an evaluation system for the cer-ebral ischemia/reperfusion(I/R)model of gerbils has shackled the application of this model.Methods:We created a symptom-oriented principle and detailed neurobehavioral scoring criteria.At different time points of reperfusion,we analyzed the alteration in locomotion by rotarod test and grip force score,infarct volume by triphenyltetrazo-lium chloride(TTC)staining,neuron loss using Nissl staining,and histological charac-teristics using hematoxylin-eosin(H&E)straining.Results:With a successful model rate of 56%,32 of the 57 gerbils operated by our method harbored typical features of cerebral I/R injury,and the mortality rate in the male gerbils was significantly higher than that in the female gerbils.The suc-cessfully prepared I/R gerbils demonstrated a significant reduction in motility and grip strength at 1 day after reperfusion;formed obvious infarction;exhibited typi-cal pathological features,such as tissue edema,neuronal atrophy and death,and vacuolated structures;and were partially recovered with the extension of reperfu-sion time.Conclusion:This study developed a new method for the unilateral common carotid artery ligation I/R model of gerbil and established a standardized evaluation system for this model,which could provide a new cerebral I/R model of gerbils with more practical applications.

Spatiotemporal changes of gross primary productivity and its response to drought in the Mongolian Plateau under climate change

Gross primary productivity(GPP)of vegetation is an important constituent of the terrestrial carbon sinks and is significantly influenced by drought.Understanding the impact of droughts on different types of vegetation GPP provides insight into the spatiotemporal variation of terrestrial carbon sinks,aiding efforts to mitigate the detrimental effects of climate change.In this study,we utilized the precipitation and temperature data from the Climatic Research Unit,the standardized precipitation evapotranspiration index(SPEI),the standardized precipitation index(SPI),and the simulated vegetation GPP using the eddy covariance-light use efficiency(EC-LUE)model to analyze the spatiotemporal change of GPP and its response to different drought indices in the Mongolian Plateau during 1982-2018.The main findings indicated that vegetation GPP decreased in 50.53% of the plateau,mainly in its northern and northeastern parts,while it increased in the remaining 49.47%area.Specifically,meadow steppe(78.92%)and deciduous forest(79.46%)witnessed a significant decrease in vegetation GPP,while alpine steppe(75.08%),cropland(76.27%),and sandy vegetation(87.88%)recovered well.Warming aridification areas accounted for 71.39% of the affected areas,while 28.53% of the areas underwent severe aridification,mainly located in the south and central regions.Notably,the warming aridification areas of desert steppe(92.68%)and sandy vegetation(90.24%)were significant.Climate warming was found to amplify the sensitivity of coniferous forest,deciduous forest,meadow steppe,and alpine steppe GPP to drought.Additionally,the drought sensitivity of vegetation GPP in the Mongolian Plateau gradually decreased as altitude increased.The cumulative effect of drought on vegetation GPP persisted for 3.00-8.00 months.The findings of this study will improve the understanding of how drought influences vegetation in arid and semi-arid areas.

Argа-Bilig theory in traditional Mongolian medicine:a theoretical and practical perspective

The concept of Arga and Bilig serves as a foundational principle in both ancient Mongolian philosophy and traditional Mongolian medicine (TMM). Arga, symbolized by brightness and associated with qualities of fire and activity, complements Bilig, symbolized by darkness and representing attributes of water and stillness. Together, these opposing forces permeate all aspects of existence, from the genesis of parenthood to the interplay of day and night. Understanding Arga-Bilig is crucial for diagnosing and treating diseases, as it illuminates the source of imbalance within the body. This review provides an overview of the significance of Arga-Bilig in Mongolian philosophy and its application in TMM, emphasizing the dynamic interplay of these opposing forces and their role in maintaining balance and harmony within the body.

A review of geophysical studies on the Mongolian Plateau

The Mongolian Plateau in Central Asia is an intracontinental tectonic system far from active plate boundaries.Despite its distance from these boundaries,the plateau is characterized by intense crustal deformation accompanied by voluminous Cenozoic volcanism and active modern seismicity.However,the intraplate deformation mechanism has long been debated owing to the scarcity of observations and contradictions between different results.In recent years,growing geophysical studies have been conducted on the Mongolian Plateau,providing constraints on its lithospheric structure and dynamics.Here,we review the geophysical research on the Mongolian Plateau over the last decade,including seismological,geodetic,gravity,magnetotelluric,and geodynamic aspects.This review aims to(a)describe crustal and mantle structures based on multiscale seismic images;(b)describe deformation patterns based on seismic anisotropy,focal mechanisms,and global positioning system(GPS)observations;and(c)discuss the mechanisms behind intraplate deformation,volcanism,and seismic activity across the Mongolian Plateau.Seismic images show that the crustal structure of the plateau has significant east-west differences.Several blocks in the western Mongolian Plateau have thick crusts,including the Altai Mountains,Hovsgol Rift,and Hangay Dome.The lithospheric deformation across the Mongolian Plateau has strong lateral variation,with NE-SW shortening in the Altai Mountains and W-E or NW-SE shear deformation in the Hangay Dome region and the eastern part.The varied deformation may result from the superposition of multiple mechanisms,including far-field stress in the Altai Mountains,mantle upwelling,and mantle flow in the Hangay Dome region.However,it is difficult to identify the geodynamics of the formation of the entire Mongolian Plateau because the deformation is too complicated,and the present models are not sufficient and are always partial.Overall,this review encompasses recent advances in seismic observations of the Mongolian Plateau,illuminates the heterogeneities in the crust and mantle structure and deformation of the plateau,and discusses the mechanisms behind the deformation,magmatism,and seismicity.

Aging Mongolian pine plantations face high risks of drought-induced growth decline:evidence from both individual tree and forest stand measurements

Discerning vulnerability differences among different aged trees to drought-driven growth decline or to mortality is critical to implement age-specific countermeasures for forest management in water-limited areas.An important species for afforestation in dry environments of northern China,Mongolian pine(Pinus sylvestris var.mongolica Litv.)has recently exhibited growth decline and dieback on many sites,particularly pronounced in old-growth plantations.However,changes in response to drought stress by this species with age as well as the underlying mechanisms are poorly understood.In this study,tree-ring data and remotely sensed vegetation data were combined to investigate variations in growth at individual tree and stand scales for young(9-13 years)and aging(35-52 years)plantations of Mongolian pine in a water-limited area of northern China.A recent decline in tree-ring width in the older plantation also had lower values in satellited-derived normalized difference vegetation indices and normalized difference water indices relative to the younger plantations.In addition,all measured growth-related metrics were strongly correlated with the self-calibrating Palmer drought severity index during the growing season in the older plantation.Sensitivity of growth to drought of the older plantation might be attributed to more severe hydraulic limitations,as reflected by their lower sapwood-and leaf-specific hydraulic conductivities.Our study presents a comprehensive view on changes of growth with age by integrating multiple methods and provides an explanation from the perspective of plant hydraulics for growth decline with age.The results indicate that old-growth Mongolian pine plantations in water-limited environments may face increased growth declines under the context of climate warming and drying.

Flooding(or breaching)of inter-connected proglacial lakes by cascading overflow in the arid region of Western Mongolia(Mt.Tsambagarav,Mongolian Altai)

This study investigates the glacial lake outburst flood(GLOF)hazards in the Tsambagarav mountain range in Western Mongolia,focusing on the Khukhnuruu Valley and its interconnected proglacial lakes.Over the last 30 years,significant glacier retreats,driven by rising temperatures and changing precipitation patterns,have led to the formation and expansion of several proglacial lakes.Fieldwork combined with satellite data and meteorological analysis was used to assess the dynamics of glacier and lake area changes,with particular focus on the flood events of July 2021.The research reveals a substantial reduction in glacier area,particularly in the Khukhnuruu E complex,where glacier area decreased by 19.3%.The study highlights the influence of increasing temperatures and summer precipitation,which have accelerated ice melt,contributing to the expansion and eventual breaching of lakes.Additionally,lake area changes were influenced by the steepness of the terrain,with steeper slopes exacerbating peak discharge during floods.Of the studied seven lakes(Lake 1 to Lake 7),Lake 1 experienced the most dramatic reduction,with a decrease in area by 73.51%and volume by 84.84%,followed by Lake 7.This study underscores the region's vulnerability to climate-induced hazards and stresses the need for a comprehensive early warning system and disaster preparedness measures to mitigate future risks.

Origin and Common Application of Mongolian Diet Therapy

This paper mainly introduces the origin of Mongolian medicine diet therapy,classic works of Mongolian medicine diet therapy and clinical conventional diet therapy through literature research,to provide a certain reference for giving reasonable diet therapy for clinical patients and improving treatment and prognosis research.

Current Status of Mongolian Medicine Treatment for Breast Hyperplasia

Through literature and clinical research,the current status of Mongolian medicine treatment for breast hyperplasia is discussed,such as oral administration of Mongolian medicine for treatment,oral administration of Mongolian medicine combined with external application for treatment,combination of Mongolian medicine acupuncture therapy and oral administration of Mongolian medicine for treatment,integrated treatment of Mongolian and Western medicine,and combination therapy of Mongolian and traditional Chinese medicine,providing new ideas and choices for clinical research.

Impacts of climate change and human activities on vegetation dynamics on the Mongolian Plateau, East Asia from 2000 to 2023

The Mongolian Plateau in East Asia is one of the largest contingent arid and semi-arid areas of the world.Under the impacts of climate change and human activities,desertification is becoming increasingly severe on the Mongolian Plateau.Understanding the vegetation dynamics in this region can better characterize its ecological changes.In this study,based on Moderate Resolution Imaging Spectroradiometer(MODIS)images,we calculated the kernel normalized difference vegetation index(kNDVI)on the Mongolian Plateau from 2000 to 2023,and analyzed the changes in kNDVI using the Theil-Sen median trend analysis and Mann-Kendall significance test.We further investigated the impact of climate change on kNDVI change using partial correlation analysis and composite correlation analysis,and quantified the effects of climate change and human activities on kNDVI change by residual analysis.The results showed that kNDVI on the Mongolian Plateau was increasing overall,and the vegetation recovery area in the southern region was significantly larger than that in the northern region.About 50.99%of the plateau showed dominant climate-driven effects of temperature,precipitation,and wind speed on kNDVI change.Residual analysis showed that climate change and human activities together contributed to 94.79%of the areas with vegetation improvement.Appropriate human activities promoted the recovery of local vegetation,and climate change inhibited vegetation growth in the northern part of the Mongolian Plateau.This study provides scientific data for understanding the regional ecological environment status and future changes and developing effective ecological protection measures on the Mongolian Plateau.

Basic philosophy of three essences theory in traditional Mongolian medicine

Traditional Mongolian medicine(TMM)integrates Tibetan and Chinese medicine with indigenous Mongolian practices,focusing on the balance of three core essences:Khii,Shar,and Badgan,derived from the Five Elements-earth,water,fire,wind,and space.These essences regulate both physical and psychological processes,and their imbalance,often caused by diet,behavior,or environment,leads to disease.TMM utilizes holistic diagnostic methods such as pulse diagnosis,visual inspection,and lifestyle evaluation to identify imbalances of three essences.Treatments include herbal remedies,dietary adjustments,behavior modification,and accessory therapies like moxibustion and massage to restore balance.This mini review examines the philosophical underpinnings,diagnostic techniques,and therapeutic strategies to Khii,Shar,and Badgan,highlighting their importance in both traditional and modern integrative healthcare.

Meta-Analysis of Efficacy and Safety of Mongolian Medical Warm Acupuncture in the Treatment of Lumbar Disc Herniation

Objective:To systematically evaluate the safety and efficacy of Mongolian medical warm acupuncture in the treatment of lumbar disc herniation(LDH).Methods:CNKI,Wanfang,VIP,Pubmed,Embase,Cochrane Library,and other databases were searched.The randomized controlled trials(RCTs)on the treatment of LDH with Mongolian medical warm acupuncture were manually searched in the Chinese Journal of Ethnic Medicine,Chinese Journal of Mongolian Medicine,Journal of Inner Mongolia University for Nationalities,and Journal of Inner Mongolia Medical University.The search time limit was from January 2000 to October 2023.RevMan5.4 software was used to analyze the included and excluded literature.Results:A total of 8 RCTs involving 1,042 patients with LDH were included,with 551 patients in the observation group and 491 patients in the control group.The results of the meta-analysis showed that a total of 8 randomized controlled trials were included in the treatment of LDH with Mongolian medical warm acupuncture compared with simple acupuncture(RR=1.18,95%CI=[1.12,1.23],P<0.00001).Conclusion:The total effective rate of Mongolian medical warm acupuncture for LDH is higher than that of simple acupuncture.However,due to the low quality of the literature included in this study,multi-dimensional,large sample size,and more rigorous clinical randomized trials are needed for further verification in the future.

基于三维运动捕捉技术构建蒙医震脑术三维虚拟仿真平台的视觉呈现

背景:基于三维运动捕捉技术可以构建精准的、客观量化的医学虚拟仿真模型,有利于临床学习者精准、深入理解和掌握各种传统疗术。目前中医虚拟仿真模型已有报道,但蒙医传统疗术的虚拟仿真模型尚未见报道。目的:构建一种基于三维运动捕捉技术的交互式三维可视化的蒙医震脑术虚拟仿真模型。方法:使用Motion Analysis三维光学运动捕捉系统和足底测力台采集蒙医专家的运动捕捉数据,在Motion Builder软件中构建震脑术三维动作模型,使用Maya软件构建角色模型并与动作模型相匹配,运动Unity3D软件构建蒙医震脑术虚拟仿真系统,系统整合蒙医震脑术操作3D动画、运动学和动力学参数信息。结果与结论:通过三维运动捕捉技术和计算机仿真重现蒙医震脑术操作,能够显示施术者和受试者的运动姿态,记录关节运动的关键空间位置参数与变化情况,得出运动过程中的运动学、动力学参数。运用交互式三维虚拟仿真技术,实现蒙医震脑术的三维虚拟仿真的视觉呈现,为蒙医震脑术手法的标准化、数字化、可视化研究奠定基础。

Cloning of Hoxc8 Promoter in Mongolian Sheep by Thermal Asymmetric Interlaced PCR

[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.

A Study on the Anxiety of Mongolian Undergraduates in Public English Class

Anxiety is a complicated psychological phenomenon in foreign language learning process, involving both cognitive and affective factors, especially for those with cross culture background. This essay focuses on the anxiety of Mongolian undergraduates in public English class, analyses the contributory factors and puts forward effective strategies on how to overcome their anxiety to achieve the goal that college public English teaching will be elevated at large.

Spatiotemporal variability of precipitation during 1961-2014 across the Mongolian Plateau

Precipitation is the dominant factor that controls vegetation growth and land-use practices in the arid and semiarid Mongolian Plateau(MP), so the spatiotemporal heterogeneity of precipitation change has been an important scientific question in the region. This study investigated the spatiotemporal characteristics of annual and seasonal precipitation across the entire MP based on monthly precipitation data from 136 meteorological stations during 1961–2014 by using a modified Mann–Kendall test, Sen's slope, Morlet Wavelet Transform, and geostatistical methods. Results show the following: 1) Annual precipitation decreased slightly from 1961 to 2014.Stations with positive and negative trends were 41.9%and 58.1%, respectively. Significant positive trends were mainly in the southwestern and northeastern regions of the plateau, whereas significant negative trends were in the northern and southeastern regions.2) Precipitation decreased at rates of-5.65 and-0.41 mm/decade in summer and autumn, respectively, but increased at 1.91 and 0.51 mm/decade in spring and winter. The contribution of spring and winter precipitation to the annual amount increased significantly, but that of summer precipitation decreased significantly. 3) A large majority of stations(80.2%) showed decreasing trends in summer,whereas 89.7% and 83.1% of stations showed increasing trends in spring and winter. The spatial distribution of trend magnitude in seasonal precipitation amount was strongly heterogeneous. 4)By climatic zones, precipitation increased in humid and arid zones, but decreased in a semiarid zone. On the whole, the MP experienced a drying trend, with significant regional differentiation and seasonal variations.

Mongolian spots:How important are they?

Mongolian spots(MS) are congenital birthmarks seen most commonly over the lumbosacral area. They are bluish-green to black in color and oval to irregular in shape. They are most commonly found in individuals of African or Asian ethnic background. Although these lesions resolve by one to two years of age, widespread, extrasacral and dark colored MS sometimes persist into adulthood. Aberrant MS over occiput, temple, mandibular area, shoulders and limbs may be confused with other dermal melanocytoses and bruises secondary to child abuse, thus necessitating documentation at birth. Although traditionally believed to be benign in nature, they have now been shown to co-exist with inborn errors of metabolism, most commonly GM1 gangliosidosis and mucopolysaccharidosis type Ⅰ(Hurler's disease), followed by mucopolysaccharidosis type Ⅱ(Hunter's syndrome), mucolipidosis, Niemann-Pick disease and mannosidosis. They have also been seen to co-exist with various vascular or other pigmented birthmarks like café-au-lait macules. Co-existing Mongolian spots and vascular birthmarks like nevus flammeus, nevus anemicus or nevus spilus is termed as phakomatosis pigmentovascularis. This review focuses on the important associations of Mongolian spots and stresses upon the importance of screening babies with extensive MS.

Experimental infection of Z:ZCLA Mongolian gerbils with human hepatitis E virus

AIM:To investigate whether Z:ZCLA Mongolian gerbils are readily susceptible to infection by human hepatitis E virus(HEV).METHODS:Z:ZCLA Mongolian gerbils were infected with a clinical HEV strain isolated from an acutehepati tis E patient,and virus pathogenesis was assessed in this host.Non-infected gerbils served as the control group.Feces samples from gerbils were collected weekly for reverse transcription-nested polymerase chain reaction.Serum anti-HEV Ig G and alanine aminotransferase(ALT) were detected by enzyme linked immunosorbent assay.At sacrifice,each animal's liver,spleen and kidney were collected for histopathologic examination.RESULTS:HEV-infected gerbils showed fatigue,with histopathological changes observed in the liver,spleen and kidney.HEV RNA was detected in fecal samples taken at day 7 after inoculation and the detectable levels lasted out to day 42 after inoculation.Interestingly,ALT levels were only moderately increased in the HEV-infected animals compared with the noninfected control group.CONCLUSION:Z:ZCLA Mongolian gerbils are susceptible to human HEV.

Identification of Sheep Endogenous Beta-Retroviruses with Uterus-Specific Expression in the Pregnant Mongolian Ewe

The sheep genome harbours approximately 20 copies of endogenous beta-retroviruses （enJSRVs）, and circumstantial evidence suggests that enJSRVs might play a role in mammalian reproduction, particularly placental morphogenesis. This study was aimed to assess the expression of mRNAs of an enJSRV and its receptor, HYAL2, in the uterus and conceptuses of Mongolian ewes throughout gestation, using real-time reverse transcription polymerase chain reaction and in situ hybridization analysis. The results showed that enJSRV and HYAL2 mRNAs were found to be expressed throughout gestation in the endometrium, chorion, placenta, and conceptus. The enJSRV mRNA was most abundant in the placenta on day 90 of pregnancy, in the endometrium on day 30 and 50, and in the chorion on day 70 and 110. However, HYAL2 mRNA was most abundant in the endometrium on day 30. These differences were all significantly different from each other （P〈0.01）. In situ hybridization showed that enJSRV and HYAL2 mRNAs were specifically expressed in endometrial luminal epithelium and glandular epithelium, trophoblastic giant binucleated cells （BNCs）, endometrial caruncles, placental cotyledons, stroma, trophectoderm, as well as multinucleated syncytia of the placenta and blood vessel endothelial cells. Collectively, little is known about the molecular mechanisms by which trophoblastic differentiation and multinucleated syncytia formation are regulated by enJSRVs. However, the temporal and spatial distributions of enJSRV expression in the uterus and conceptus indicate that differentiation of BNCs and the formation of a multinucleated syncytiotrophoblast involve enJSRV and possibly its cellular receptor, HYAL2. Therefore, enJSRV and HYAL2 appear to play important roles in the female reproductive physiology in this breed of sheep.

The effect of mast cell on the induction of Helicobacter pylori infection in Mongolian gerbils

INTRODUCTION Since 1982,Helicobacter pylori(Hp) has been successfully isolated and cultured,and the fact many diseases such as gastritis,peptic ulcer,gastric carcinoma

Monitoring desertification processes in Mongolian Plateau using MODIS tasseled cap transformation and TGSI time series

Most remote sensing studies assess the desertification using vegetation monitoring method. But it has the insufficient precision of vegetation monitoring for the limited vegetation cover of the desertification region. Therefore, it offers an alternative approach for the desertificafon research to assess sand dune and sandy land change using remote sensing in the desertification region. In this study, the indices derived from the well-known tasseled cap transformation （TCT）, tasseled cap angle （TCA）, disturbance index （DI）, process indicator （PI）, and topsoil grain size index （TGSI） were integrated to monitor and assess the desertification at the thirteen study sites including sand dunes and sandy lands distributed in the Mongolian Plateau （MP） from 2000 to 2015. A decision tree was used to classify the desertification on a regional scale. The average overall accuracy of 2000, 2005, 2010 and 2015 desertification classification was higher than 90%. Results from this study indicated that integration of the advantages of TCA, DI and TGSI could better assess the desertification. During the last 16 years, Badain Jaran Desert, Tengger Desert, and Ulan Buh Desert showed a relative stabilization. Otindag Sandy Land and the deserts of Khar Nuur, Ereen Nuur, Tsagan Nuur, Khongoryn Els, Hobq, and Mu Us showed a slow increasing of desertification, whereas Bayan Gobi, Horqin and Hulun Buir sandy lands showed a slow decreasing of desertification. Compared with the other 11 sites, the fine sand dunes occupied the majority of the Tengger Desert, and the coarse sandy land occupied the majority of the Horqin Sandy Land. Our findings on a three or four years＇ periodical fluctuated changes in the desertification may possibly reflect changing precipitation and soil moisture in the MR Further work to link the TCA, DI, TGSI, and PI values with the desertification characteristics is recommended to set the thresholds and improve the assessment accuracy with field investigation.