Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point...Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point that relates the microbeam＇s location to the microscope＇s plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam, which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup.展开更多
In-situ calibrations of weather stations are usually performed by positioning standard instruments close to the station under calibration and comparing the obtained results. This procedure could be useful to evaluate ...In-situ calibrations of weather stations are usually performed by positioning standard instruments close to the station under calibration and comparing the obtained results. This procedure could be useful to evaluate the proper functioning of the monitoring equipments, but do not allowed the determination of a calibration curve that allow the corrections of the acquired parameters. Thus, the development of a dedicated facility for in-situ calibration of weather stations, enabling simultaneous generation of a wide range of temperatures and pressures could offer important improvements in this framework, particularly if this facility is applied to high mountains monitoring stations where the weather stations calibrations could be very difficult. This paper will present the calibration chamber developed in the framework of the EMRP-METEOMET (Metrology for Meteorology) Project, which aims is to bring metrological traceability to high altitude meteorological instruments and through this experience will provide a general overview on the importance of the application of this methodology at different levels.展开更多
文摘Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point that relates the microbeam＇s location to the microscope＇s plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam, which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup.
文摘In-situ calibrations of weather stations are usually performed by positioning standard instruments close to the station under calibration and comparing the obtained results. This procedure could be useful to evaluate the proper functioning of the monitoring equipments, but do not allowed the determination of a calibration curve that allow the corrections of the acquired parameters. Thus, the development of a dedicated facility for in-situ calibration of weather stations, enabling simultaneous generation of a wide range of temperatures and pressures could offer important improvements in this framework, particularly if this facility is applied to high mountains monitoring stations where the weather stations calibrations could be very difficult. This paper will present the calibration chamber developed in the framework of the EMRP-METEOMET (Metrology for Meteorology) Project, which aims is to bring metrological traceability to high altitude meteorological instruments and through this experience will provide a general overview on the importance of the application of this methodology at different levels.
