The combined use of morphological identification and phylogenetic characterization employing primers that target the 16S rDNA region led to the identification of ten isolates belonging to eight cyanobacterial genera i...The combined use of morphological identification and phylogenetic characterization employing primers that target the 16S rDNA region led to the identification of ten isolates belonging to eight cyanobacterial genera in the Nile River. 16S-23S ITS region was amplified to confirm two isolates to be affiliated to genus Nostoc. Using MALDI-TOF/MS, we detected the production of the hepatotoxic demethylated MC-LR by one isolate that clustered together with the genus Nostoc. Protein phosphatase inhibition assay has confirmed toxicity. Our results add to the rising importance of Nostoc as a hepatotoxin-producing cyanobacterium. Furthermore, our results stress that water municipalities in the studied region need to assess the potential threat of toxic cyanobacteria that may pose to human health and economy.展开更多
根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上...根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。展开更多
[Objective]The experiment aimed to explore a new way for observing surface structure of Nostoc sphaeroides Kutzing. [Method] The scanning electron microscope was used to observe the epidermal ultrastructure of wild an...[Objective]The experiment aimed to explore a new way for observing surface structure of Nostoc sphaeroides Kutzing. [Method] The scanning electron microscope was used to observe the epidermal ultrastructure of wild and cultured Nostoc sphaeroides Kutzing. [ Result] The epidermis of wild and cultured Nostoc sphaeroides Kutzing showed mixture structure of fibril colloid which was reticular arranged. The difference between wild and cultured Nostoc sphaeroides Kutzing was that the outer epidermis of cultured Nostoc sphaeroides Kutzing had trichome distribution but the wild Nostoc sphaeroides Kutzing did not has such distribution. The obsevation results of under smaller than 10 μm by scanning electron microscope was touched thick and showed many folds and distortions. [ Conclusion] The scanning electron microscope was an effective way to study development of Nostoc sphaeroides Kutzing colony and it was worth popularizing.展开更多
文摘The combined use of morphological identification and phylogenetic characterization employing primers that target the 16S rDNA region led to the identification of ten isolates belonging to eight cyanobacterial genera in the Nile River. 16S-23S ITS region was amplified to confirm two isolates to be affiliated to genus Nostoc. Using MALDI-TOF/MS, we detected the production of the hepatotoxic demethylated MC-LR by one isolate that clustered together with the genus Nostoc. Protein phosphatase inhibition assay has confirmed toxicity. Our results add to the rising importance of Nostoc as a hepatotoxin-producing cyanobacterium. Furthermore, our results stress that water municipalities in the studied region need to assess the potential threat of toxic cyanobacteria that may pose to human health and economy.
文摘根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。
文摘[Objective]The experiment aimed to explore a new way for observing surface structure of Nostoc sphaeroides Kutzing. [Method] The scanning electron microscope was used to observe the epidermal ultrastructure of wild and cultured Nostoc sphaeroides Kutzing. [ Result] The epidermis of wild and cultured Nostoc sphaeroides Kutzing showed mixture structure of fibril colloid which was reticular arranged. The difference between wild and cultured Nostoc sphaeroides Kutzing was that the outer epidermis of cultured Nostoc sphaeroides Kutzing had trichome distribution but the wild Nostoc sphaeroides Kutzing did not has such distribution. The obsevation results of under smaller than 10 μm by scanning electron microscope was touched thick and showed many folds and distortions. [ Conclusion] The scanning electron microscope was an effective way to study development of Nostoc sphaeroides Kutzing colony and it was worth popularizing.
