Objective: To test whether the down-regulation of Notchl gene expression by curcumin could inhibit cell growth and induce apoptosis, which may be associated mechanistically with the down-regulation of NF-κB in breas...Objective: To test whether the down-regulation of Notchl gene expression by curcumin could inhibit cell growth and induce apoptosis, which may be associated mechanistically with the down-regulation of NF-κB in breast cancer cells. Methods: Breast cancer cell lines MDA-MB-231 were cultured in vitro and treated with different dosages of curcumin (0, 1.25, 5.0, 20.0μmol/L) for dose-dependent assay and different time (0, 24, 48, 72 h) at the dosage of 5.0μmol/L for time course assay. The changes of the mRNA and protein expression of Notchl and NF-κB were measured by RT-PCR and Western Blot, and MTT assay was used to measure the change of proliferation. Results: The mRNA and protein levels of Notchl and NF-κB were decreased significantly in human breast cancer cell line with the increase of dosage of curcumin(P〈0.05), and with the extension of time course(P〈0.05). These changes suggested a dose- and time-dependent manner. The proliferation rate of cells also was significantly inhibited(P〈0.05). Conclusion: The current results show that the Notch-1 signaling pathway is associated mechanistically with NF-κB activity during curcumin-induced cell growth inhibition and apoptosis of breast cancer cells. These results suggest that the down-regulation of Notch signaling by curcumin may be a novel strategy for the treatment of patients with breast cancer.展开更多
Objective To study the mechanistic role of myeloid-specific Notchl knockout inhibiting STING signaling to regulate hepatocyte lipophagy.Methods A mouse model of nonalcoholic steatohepatitis(NASH)was established using ...Objective To study the mechanistic role of myeloid-specific Notchl knockout inhibiting STING signaling to regulate hepatocyte lipophagy.Methods A mouse model of nonalcoholic steatohepatitis(NASH)was established using a high-fat diet(HFD)and mouse bone marrow-derived macrophages(BMMs).Primary hepatocytes were isolated to construct a co-culture system.展开更多
文摘Objective: To test whether the down-regulation of Notchl gene expression by curcumin could inhibit cell growth and induce apoptosis, which may be associated mechanistically with the down-regulation of NF-κB in breast cancer cells. Methods: Breast cancer cell lines MDA-MB-231 were cultured in vitro and treated with different dosages of curcumin (0, 1.25, 5.0, 20.0μmol/L) for dose-dependent assay and different time (0, 24, 48, 72 h) at the dosage of 5.0μmol/L for time course assay. The changes of the mRNA and protein expression of Notchl and NF-κB were measured by RT-PCR and Western Blot, and MTT assay was used to measure the change of proliferation. Results: The mRNA and protein levels of Notchl and NF-κB were decreased significantly in human breast cancer cell line with the increase of dosage of curcumin(P〈0.05), and with the extension of time course(P〈0.05). These changes suggested a dose- and time-dependent manner. The proliferation rate of cells also was significantly inhibited(P〈0.05). Conclusion: The current results show that the Notch-1 signaling pathway is associated mechanistically with NF-κB activity during curcumin-induced cell growth inhibition and apoptosis of breast cancer cells. These results suggest that the down-regulation of Notch signaling by curcumin may be a novel strategy for the treatment of patients with breast cancer.
文摘Objective To study the mechanistic role of myeloid-specific Notchl knockout inhibiting STING signaling to regulate hepatocyte lipophagy.Methods A mouse model of nonalcoholic steatohepatitis(NASH)was established using a high-fat diet(HFD)and mouse bone marrow-derived macrophages(BMMs).Primary hepatocytes were isolated to construct a co-culture system.