Pentafluorophenyl diphenylphosphate was found to be a novel convenient coupling reagent for the peptide synthesis with several advantages.Higher yield and lower racemization were found with this reagent than DCCI.
The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) we...The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) were fractionated, characterized and quantified by liquid chromatography on a C-18 column using UV detection. This revealed the presence, in femtomoles per cell, of glutathione (8.30 ± 0.73), cysteine (2.71 ± 0.04) and cysteinylglycine (0.83 ± 0.10), accounting for the bulk of the thiol present. Further analysis of the cell extracts using a novel and sensitive mass spectrometry technique allowed the detection of low level of an additional derivative which was identified as cysteinylglycerate using NMRspectroscopy.展开更多
In continuation of research to explore the applied potential of DMF-like ionic liquid, the ionic liquid version of N,N-dimethyliminiumchloride （Vilsmier reagent） has been synthesized from DMF-like ionic liquid and t...In continuation of research to explore the applied potential of DMF-like ionic liquid, the ionic liquid version of N,N-dimethyliminiumchloride （Vilsmier reagent） has been synthesized from DMF-like ionic liquid and tested effectively for its capacity to achieve more useful organic transformations. The results show that DMF-like ionic liquid is world＇s first task specific ionic liquid which has catalyzed numerous diverse type of reaction and is multipurpose in its application. Thus a new term for this DMF-like ionic liquid has been coined that is DMF-like ＂multipurpose＂ ionic liquid.展开更多
Recently we and others have reported that palladium could insert into the carbon-oxygen bond of phenyl fluoroalkanesulfonates,ArOSOR（1）,which could be convenientlyprepared from phenols.Thus,alkenylation and alkynyla...Recently we and others have reported that palladium could insert into the carbon-oxygen bond of phenyl fluoroalkanesulfonates,ArOSOR（1）,which could be convenientlyprepared from phenols.Thus,alkenylation and alkynylation of phenols could be accom-plished via the Heck reaction of 1,and moreover,some procedures for alkylation of phenolshave been provided by the cross-coupling reaction of 1 with organozincs and organotins,In order to extend the scope of the coupling reaction of 1,we are attracted by the palladium-展开更多
文摘Pentafluorophenyl diphenylphosphate was found to be a novel convenient coupling reagent for the peptide synthesis with several advantages.Higher yield and lower racemization were found with this reagent than DCCI.
文摘The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) were fractionated, characterized and quantified by liquid chromatography on a C-18 column using UV detection. This revealed the presence, in femtomoles per cell, of glutathione (8.30 ± 0.73), cysteine (2.71 ± 0.04) and cysteinylglycine (0.83 ± 0.10), accounting for the bulk of the thiol present. Further analysis of the cell extracts using a novel and sensitive mass spectrometry technique allowed the detection of low level of an additional derivative which was identified as cysteinylglycerate using NMRspectroscopy.
文摘In continuation of research to explore the applied potential of DMF-like ionic liquid, the ionic liquid version of N,N-dimethyliminiumchloride （Vilsmier reagent） has been synthesized from DMF-like ionic liquid and tested effectively for its capacity to achieve more useful organic transformations. The results show that DMF-like ionic liquid is world＇s first task specific ionic liquid which has catalyzed numerous diverse type of reaction and is multipurpose in its application. Thus a new term for this DMF-like ionic liquid has been coined that is DMF-like ＂multipurpose＂ ionic liquid.
文摘Recently we and others have reported that palladium could insert into the carbon-oxygen bond of phenyl fluoroalkanesulfonates,ArOSOR（1）,which could be convenientlyprepared from phenols.Thus,alkenylation and alkynylation of phenols could be accom-plished via the Heck reaction of 1,and moreover,some procedures for alkylation of phenolshave been provided by the cross-coupling reaction of 1 with organozincs and organotins,In order to extend the scope of the coupling reaction of 1,we are attracted by the palladium-
