AIM:To assess the prognostic significance of nuclear factor-kB (NF-kB) and its target genes in gastric cancer. METHODS:The tumor tissues of 115 patients with gastric cancer were immunohistochemically evaluated using m...AIM:To assess the prognostic significance of nuclear factor-kB (NF-kB) and its target genes in gastric cancer. METHODS:The tumor tissues of 115 patients with gastric cancer were immunohistochemically evaluated using monoclonal antibodies against NF-kB RelA. Preoperative serum levels of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6) were assessed via enzyme-linked immuno-sorbent assay. C-reactive protein (CRP) and serum amyloid A (SAA) were measured via immunotrubidimetry. RESULTS:Positive rate of NF-kB RelA was 42.6%. NF-kB RelA expression in tumor tissues was also related to serum levels of IL-6 (P = 0.044) and CRP (P = 0.010). IL-6, SAA, CRP were related to depth of invasion, VEGF and SAA were correlated with lymph node metastasis. IL-6, VEGF, SAA and CRP were related to the stage. Univariate analysis demonstrated that immunostaining of NF-kB RelA, levels of IL-6, VEGF, SAA were significantly related with both disease free survival and over-all survival (OS). Multivariate analysis verified that NF-kB RelA [hazard ratio (HR): 3.40, P = 0.024] and SAA (HR: 3.39, P = 0.045) were independently associated with OS. CONCLUSION: Increased expression of NF-kB RelA and high levels of serum SAA were associated with poor OS in gastric cancer patients.展开更多
Objective To observe the different expression of NF-kBp65 and cyclinD1 during oral carcinogenesis and to analyze the relationship between the abnormal expression of NF-kBp65 , cyclinD1, and the occurrence and developm...Objective To observe the different expression of NF-kBp65 and cyclinD1 during oral carcinogenesis and to analyze the relationship between the abnormal expression of NF-kBp65 , cyclinD1, and the occurrence and development of oral carcinogenesis. Methods The streptavidin-biotin-peroxidase （S-P） immunohistochemical method was employed to detect the expression of NF-kBp65 and cyclinD1 protein in 38 rat tongue carcinogenesis specimens induced by 4-nitroquinoline 1-oxide. Results With the progress of tongue carcinogenesis, the expression of NF-kBp65, cyclinD1 was up-regulated. In normal, mild epithelial dysplasia, moderate epithelial dysplasia, severe epithelial dysplasia, carcinoma in situ and squamous cell carcinoma （SCC）, the positive rate of NF-kBp65 was 20%, 20%, 50%, 62.5%, 50% and 83.33%, respectively. There was significant differences between normal and SCC （ P 〈 0. 05 ） ; while the level of cyclinD1 was 20%, 60%, 62. 5%, 87. 5% , 100% and 83. 33%, respec- tively. There was significant differences between normal and severe epithelial dysplasia, carcinoma in situ and SCC （ P 〈0.01 or P 〈 0. 05 ）. There was a significant correlation between the increased levels of NF-kBp65, cyclinD1 and histopathological grade. The positive expression of NF-kBp65 was also associated with cyclinD1 in SCC （ r = 0. 7353, P 〈 0. 05 ）. Conclusion The up-expression of NF-kBp65 and cyclinD1 protein may be correlated to the occurrence and the development of oral carcinoma ; activated NF-kB plays an important role in the overexpression of cyclinD1. Furthermore, NF-kB and cyclinD1 may be the useful biomarker of oral precancerous lesion.展开更多
基金Supported by The Dong-A University Research Fund
文摘AIM:To assess the prognostic significance of nuclear factor-kB (NF-kB) and its target genes in gastric cancer. METHODS:The tumor tissues of 115 patients with gastric cancer were immunohistochemically evaluated using monoclonal antibodies against NF-kB RelA. Preoperative serum levels of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6) were assessed via enzyme-linked immuno-sorbent assay. C-reactive protein (CRP) and serum amyloid A (SAA) were measured via immunotrubidimetry. RESULTS:Positive rate of NF-kB RelA was 42.6%. NF-kB RelA expression in tumor tissues was also related to serum levels of IL-6 (P = 0.044) and CRP (P = 0.010). IL-6, SAA, CRP were related to depth of invasion, VEGF and SAA were correlated with lymph node metastasis. IL-6, VEGF, SAA and CRP were related to the stage. Univariate analysis demonstrated that immunostaining of NF-kB RelA, levels of IL-6, VEGF, SAA were significantly related with both disease free survival and over-all survival (OS). Multivariate analysis verified that NF-kB RelA [hazard ratio (HR): 3.40, P = 0.024] and SAA (HR: 3.39, P = 0.045) were independently associated with OS. CONCLUSION: Increased expression of NF-kB RelA and high levels of serum SAA were associated with poor OS in gastric cancer patients.
基金Supported by Shanghai Science and Technology Commission,China (03JC14037)
文摘Objective To observe the different expression of NF-kBp65 and cyclinD1 during oral carcinogenesis and to analyze the relationship between the abnormal expression of NF-kBp65 , cyclinD1, and the occurrence and development of oral carcinogenesis. Methods The streptavidin-biotin-peroxidase （S-P） immunohistochemical method was employed to detect the expression of NF-kBp65 and cyclinD1 protein in 38 rat tongue carcinogenesis specimens induced by 4-nitroquinoline 1-oxide. Results With the progress of tongue carcinogenesis, the expression of NF-kBp65, cyclinD1 was up-regulated. In normal, mild epithelial dysplasia, moderate epithelial dysplasia, severe epithelial dysplasia, carcinoma in situ and squamous cell carcinoma （SCC）, the positive rate of NF-kBp65 was 20%, 20%, 50%, 62.5%, 50% and 83.33%, respectively. There was significant differences between normal and SCC （ P 〈 0. 05 ） ; while the level of cyclinD1 was 20%, 60%, 62. 5%, 87. 5% , 100% and 83. 33%, respec- tively. There was significant differences between normal and severe epithelial dysplasia, carcinoma in situ and SCC （ P 〈0.01 or P 〈 0. 05 ）. There was a significant correlation between the increased levels of NF-kBp65, cyclinD1 and histopathological grade. The positive expression of NF-kBp65 was also associated with cyclinD1 in SCC （ r = 0. 7353, P 〈 0. 05 ）. Conclusion The up-expression of NF-kBp65 and cyclinD1 protein may be correlated to the occurrence and the development of oral carcinoma ; activated NF-kB plays an important role in the overexpression of cyclinD1. Furthermore, NF-kB and cyclinD1 may be the useful biomarker of oral precancerous lesion.
