In this review,we discuss recent advances in nucleic acid-based therapeutic technologies that target hepatitis C virus(HCV)infection.Because the HCV genome is present exclusively in RNA form during replication,various...In this review,we discuss recent advances in nucleic acid-based therapeutic technologies that target hepatitis C virus(HCV)infection.Because the HCV genome is present exclusively in RNA form during replication,various nucleic acid-based therapeutic approaches targeting the HCV genome,such as ribozymes,aptamers,siRNAs,and antisense oligonucleotides,have been suggested as potential tools against HCV.Nucleic acids are potentially immunogenic and typically require a delivery tool to be utilized as therapeutics.These limitations have hampered the clinical development of nucleic acid-based therapeutics.However,despite these limitations,nucleic acid-based therapeutics has clinical value due to their great specificity,easy and large-scale synthesis with chemical methods,and pharmaceutical flexibility.Moreover,nucleic acid therapeutics are expected to broaden the range of targetable molecules essential for the HCV replication cycle,and therefore they may prove to be more effective than existing therapeutics,such as interferon-αand ribavirin combination therapy.This review focuses on the current status and future prospects of ribozymes,aptamers,siRNAs,and antisense oligonucleotides as therapeutic reagents against HCV.展开更多
The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting...The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments,saving people's lives and preventing epidemics.It is important to establish a quick standard diagnostic test for the detection of the infectious disease(COVID-19)to prevent subsequent secondary spread.Polymerase chain reaction(PCR)is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity.Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler opera-tion.A variety of improved or new approaches also have been developed.This review summarizes the currently available detection methods for coronavirus nucleic acid.It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coro-navirus infection.展开更多
Infectious diseases,mostly caused by bacteria and viruses but also a result of fungal and parasitic infection,have been one of the most important public health concerns throughout human history.The first step in comba...Infectious diseases,mostly caused by bacteria and viruses but also a result of fungal and parasitic infection,have been one of the most important public health concerns throughout human history.The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost.Many kinds of diagnostics have been developed,such as pathogen culture,biochemical tests and serological tests,to help detect and fight against the causative agents of diseases.However,these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results.Nucleic acid-based diagnostics,detecting pathogens through the identification of their genomic sequences,have shown promise to overcome the above limitations and become more widely adopted in clinical tests.Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage.We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics.展开更多
基金Supported by National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT and Future Planning No.2012M3A9B6055200,No.2013R1A2A2A01004649
文摘In this review,we discuss recent advances in nucleic acid-based therapeutic technologies that target hepatitis C virus(HCV)infection.Because the HCV genome is present exclusively in RNA form during replication,various nucleic acid-based therapeutic approaches targeting the HCV genome,such as ribozymes,aptamers,siRNAs,and antisense oligonucleotides,have been suggested as potential tools against HCV.Nucleic acids are potentially immunogenic and typically require a delivery tool to be utilized as therapeutics.These limitations have hampered the clinical development of nucleic acid-based therapeutics.However,despite these limitations,nucleic acid-based therapeutics has clinical value due to their great specificity,easy and large-scale synthesis with chemical methods,and pharmaceutical flexibility.Moreover,nucleic acid therapeutics are expected to broaden the range of targetable molecules essential for the HCV replication cycle,and therefore they may prove to be more effective than existing therapeutics,such as interferon-αand ribavirin combination therapy.This review focuses on the current status and future prospects of ribozymes,aptamers,siRNAs,and antisense oligonucleotides as therapeutic reagents against HCV.
基金financial support from the National Natural Science Foundation of China(Grant 81973281)the Fundamental Research Funds for the Central Universities(2019FZA7017)Leading Talent of“Ten Thousand Plan”-National High-Level Talents SpecialSupport Plan。
文摘The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments,saving people's lives and preventing epidemics.It is important to establish a quick standard diagnostic test for the detection of the infectious disease(COVID-19)to prevent subsequent secondary spread.Polymerase chain reaction(PCR)is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity.Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler opera-tion.A variety of improved or new approaches also have been developed.This review summarizes the currently available detection methods for coronavirus nucleic acid.It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coro-navirus infection.
基金supported by grants from the National Key Technology R&D Program(No.2008BAK41B01-5)the National Science and Technology Major Project—Development New Genetically Modified Organism(No.2009ZX08012-006B).
文摘Infectious diseases,mostly caused by bacteria and viruses but also a result of fungal and parasitic infection,have been one of the most important public health concerns throughout human history.The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost.Many kinds of diagnostics have been developed,such as pathogen culture,biochemical tests and serological tests,to help detect and fight against the causative agents of diseases.However,these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results.Nucleic acid-based diagnostics,detecting pathogens through the identification of their genomic sequences,have shown promise to overcome the above limitations and become more widely adopted in clinical tests.Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage.We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics.