目的:研究幽门螺杆菌(Helicobacter pylori,H.pylori)感染对人血清及胃组织核组蛋白2(nucleobindin 2,NUCB2)/nesfatin-1表达的影响.方法:收集行胃镜检查的83例无症状体检者的空腹血清及胃窦黏膜,经13C呼气试验、快速尿素酶实验及组织切...目的:研究幽门螺杆菌(Helicobacter pylori,H.pylori)感染对人血清及胃组织核组蛋白2(nucleobindin 2,NUCB2)/nesfatin-1表达的影响.方法:收集行胃镜检查的83例无症状体检者的空腹血清及胃窦黏膜,经13C呼气试验、快速尿素酶实验及组织切片Warth-Starry银染色三者共同确认分为H.pylori阳性组与H.pylori阴性组,采用酶联免疫吸附法及实时荧光定量PCR法分别测定血清NUCB2/nesfatin-1蛋白浓度及胃黏膜NUCB2 m RNA的表达量.结果:血清NUCB2/nesfatin-1蛋白浓度在H.pylori阳性组与H.pylori阴性组无明显差异(2.267 ng/m L±0.201 ng/m L vs 2.298 ng/m L±0.275 ng/m L,P>0.05);胃黏膜NUCB2 m RNA相对表达量在H.pylori阳性组明显高于H.pylori阴性组(1.336±0.324 vs 0.914±0.171,P<0.01).结论:H.pylori感染可导致胃黏膜NUCB2m R N A表达上调,但不影响感染者血清NUCB2/nesfatin-1浓度.展开更多
AIM: To observe the regional distributions and morphological features of nesfatin-1/nucleobindin-2 (NUCB2) immunoreactive (IR) cells in the rodent digestive system. METHODS: Paraffin-embedded sections of seven organs ...AIM: To observe the regional distributions and morphological features of nesfatin-1/nucleobindin-2 (NUCB2) immunoreactive (IR) cells in the rodent digestive system. METHODS: Paraffin-embedded sections of seven organs (pancreas, stomach, duodenum, esophagus, liver, small intestine and colon) dissected from sprague-dawley (SD) rats and institute of Cancer Research (ICR) mice were prepared. The regional distributions of nesfatin-1/NUCB2 IR cells were observed by immunohistochemical staining. The morphological features of the nesfatin-1/NUCB2 IR cells were evaluated by hematoxylin and eosin (HE) staining. Fresh tissues of the seven organs were prepared for Western blotting to analyze the relative protein levels of NUCB2 in each organ.RESULTS: Immunohistochemical staining showed that the nesfatin-1/NUCB2 IR cells were localized in the central part of the pancreatic islets, the lower third and middle portion of the gastric mucosal gland, and the submucous layer of the duodenum in SD rats and ICR mice. HE staining revealed that the morphological features of nesfatin-1/NUCB2 IR cells were mainly islet cells in the pancreas, endocrine cells in the stomach, and Brunner’s glands in the duodenum. Western blotting revealed that NUCB2 protein expression was higher in the pancreas, stomach and duodenum than in the esophagus, liver, small intestine and colon (P = 0.000). CONCLUSION: Nesfatin-1/NUCB2 IR cells are expressed in the pancreas, stomach and duodenum in rodents. These cells may play an important role in the physiological regulation of carbohydrate metabolism, gastrointestinal function and nutrient absorption.展开更多
文摘目的:研究幽门螺杆菌(Helicobacter pylori,H.pylori)感染对人血清及胃组织核组蛋白2(nucleobindin 2,NUCB2)/nesfatin-1表达的影响.方法:收集行胃镜检查的83例无症状体检者的空腹血清及胃窦黏膜,经13C呼气试验、快速尿素酶实验及组织切片Warth-Starry银染色三者共同确认分为H.pylori阳性组与H.pylori阴性组,采用酶联免疫吸附法及实时荧光定量PCR法分别测定血清NUCB2/nesfatin-1蛋白浓度及胃黏膜NUCB2 m RNA的表达量.结果:血清NUCB2/nesfatin-1蛋白浓度在H.pylori阳性组与H.pylori阴性组无明显差异(2.267 ng/m L±0.201 ng/m L vs 2.298 ng/m L±0.275 ng/m L,P>0.05);胃黏膜NUCB2 m RNA相对表达量在H.pylori阳性组明显高于H.pylori阴性组(1.336±0.324 vs 0.914±0.171,P<0.01).结论:H.pylori感染可导致胃黏膜NUCB2m R N A表达上调,但不影响感染者血清NUCB2/nesfatin-1浓度.
基金Supported by The Science and Technology Foundation ofHealth Department of Jiangsu Province, No. H200607Janssen Research Council China Research Fund (2008)
文摘AIM: To observe the regional distributions and morphological features of nesfatin-1/nucleobindin-2 (NUCB2) immunoreactive (IR) cells in the rodent digestive system. METHODS: Paraffin-embedded sections of seven organs (pancreas, stomach, duodenum, esophagus, liver, small intestine and colon) dissected from sprague-dawley (SD) rats and institute of Cancer Research (ICR) mice were prepared. The regional distributions of nesfatin-1/NUCB2 IR cells were observed by immunohistochemical staining. The morphological features of the nesfatin-1/NUCB2 IR cells were evaluated by hematoxylin and eosin (HE) staining. Fresh tissues of the seven organs were prepared for Western blotting to analyze the relative protein levels of NUCB2 in each organ.RESULTS: Immunohistochemical staining showed that the nesfatin-1/NUCB2 IR cells were localized in the central part of the pancreatic islets, the lower third and middle portion of the gastric mucosal gland, and the submucous layer of the duodenum in SD rats and ICR mice. HE staining revealed that the morphological features of nesfatin-1/NUCB2 IR cells were mainly islet cells in the pancreas, endocrine cells in the stomach, and Brunner’s glands in the duodenum. Western blotting revealed that NUCB2 protein expression was higher in the pancreas, stomach and duodenum than in the esophagus, liver, small intestine and colon (P = 0.000). CONCLUSION: Nesfatin-1/NUCB2 IR cells are expressed in the pancreas, stomach and duodenum in rodents. These cells may play an important role in the physiological regulation of carbohydrate metabolism, gastrointestinal function and nutrient absorption.
