The toxicity of naphthalene to a freshwater microalga, Chlorella pyrenoidosa, and the subsequent recovery of algae from the damage were investigated under two nutrient conditions, either enriched with nitrogen (N) a...The toxicity of naphthalene to a freshwater microalga, Chlorella pyrenoidosa, and the subsequent recovery of algae from the damage were investigated under two nutrient conditions, either enriched with nitrogen (N) and phosphorus (P), or starved of N and E Results showed that C. pyrenoidosa was more sensitive to naphthalene under N,P-enriched condition, and the inhibitory rate generally increased at first and then decreased gradually with the evaporation of naphthalene under both nutrient conditions. Enriched N, P reduced the inhibitory rate at initial naphthalene concentration of 5 and 10 mg/L, but enhanced it at 100 mg/L, at which more severe ultrastructure damages were found than those under N,P-starved condition. Observed damages included partly or totally disappearance of nucleolus, nuclear, and plasma membranes. According to the chlorophyll content and cell density measurements, C. pyrenoidosa could recover from naphthalene damage with initial concentrations ≤ 50 mg/L in 7 days under both nutrient conditions, while they could not recover if the initial concentration of naphthalene was at 100 mg/L. Under the N,P-starved condition, the inability of C. pyrenoidosa to recover from the naphthalene damage was consistent with the results of high inhibitory rate, low value of specific growth rate (SGR, 0.05 day-l), and the severe destruction of cell structure. However, under the N,P-enriched conditions, the observed lower inhibitory rate, higher value of SGR (0.55 day^-1), and the intact cell structure of most cells suggested that algae could potentially recover from the naphthalene damage.展开更多
Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and pro...Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp.ICE-L grown in NH_4 Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH_4NO_3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids(PUFAs)production, NH_4NO_3 and NH_4 Cl media performed better than nitrogen-deficiency and KNO_3 media.Furthermore, NH_4NO_3 and NH_4 Cl media elucidated better results on C18:3 and C20:5 productions while KNO_3and-N conditions were better in C16:0, C18:1 and C18:2, comparatively.展开更多
基金supported by the National Natural Science Foundation of China(No. 20890110,20977075)the Technology Research and Development Program of Tianjin Agriculture University(No. 2007003)
文摘The toxicity of naphthalene to a freshwater microalga, Chlorella pyrenoidosa, and the subsequent recovery of algae from the damage were investigated under two nutrient conditions, either enriched with nitrogen (N) and phosphorus (P), or starved of N and E Results showed that C. pyrenoidosa was more sensitive to naphthalene under N,P-enriched condition, and the inhibitory rate generally increased at first and then decreased gradually with the evaporation of naphthalene under both nutrient conditions. Enriched N, P reduced the inhibitory rate at initial naphthalene concentration of 5 and 10 mg/L, but enhanced it at 100 mg/L, at which more severe ultrastructure damages were found than those under N,P-starved condition. Observed damages included partly or totally disappearance of nucleolus, nuclear, and plasma membranes. According to the chlorophyll content and cell density measurements, C. pyrenoidosa could recover from naphthalene damage with initial concentrations ≤ 50 mg/L in 7 days under both nutrient conditions, while they could not recover if the initial concentration of naphthalene was at 100 mg/L. Under the N,P-starved condition, the inability of C. pyrenoidosa to recover from the naphthalene damage was consistent with the results of high inhibitory rate, low value of specific growth rate (SGR, 0.05 day-l), and the severe destruction of cell structure. However, under the N,P-enriched conditions, the observed lower inhibitory rate, higher value of SGR (0.55 day^-1), and the intact cell structure of most cells suggested that algae could potentially recover from the naphthalene damage.
基金The National Natural Science Foundation of China under contract No.41576187the National Natural Science Foundation of China–Shandong Joint Fund under contract No.U1406402+6 种基金the Basic Scientific Fund for National Public Research Institutes of China under contract No.2015G10the Polar Strategic Foundation of China under contract No.20150303the Public Science and Technology Research Funds Projects of Ocean under contract No.201405015the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology and the Science under contract No.2015ASKJ02the Science and Technology Planning Project of Shandong Province under contract No.2014GHY115003the Major Projects of Independent Innovation Achievements Transformation in Shandong Province under contract No.2014ZZCX06202Qingdao Entrepreneurship and Innovation Pioneers Program under contract No.15-10-3-15-(44)-zch
文摘Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp.ICE-L grown in NH_4 Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH_4NO_3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids(PUFAs)production, NH_4NO_3 and NH_4 Cl media performed better than nitrogen-deficiency and KNO_3 media.Furthermore, NH_4NO_3 and NH_4 Cl media elucidated better results on C18:3 and C20:5 productions while KNO_3and-N conditions were better in C16:0, C18:1 and C18:2, comparatively.