Denitrification-induced nitrogen(N) losses from croplands may be greatly increased by intensive fertilization.However,the accurate quantification of these losses is still challenging due to insufficient available in s...Denitrification-induced nitrogen(N) losses from croplands may be greatly increased by intensive fertilization.However,the accurate quantification of these losses is still challenging due to insufficient available in situ measurements of soil dinitrogen(N) emissions.We carried out two one-week experiments in a maize-wheat cropping system with calcareous soil using theN gas-flux(NGF) method to measure in situ Nfluxes following urea application.Applications ofN-labeled urea(99 atom%,130-150 kg N ha) were followed by irrigation on the 1 st,3 rd,and 5 th days after fertilization(DAF 1,3,and 5,respectively).The detection limits of the soil Nfluxes were 163-1 565,81-485,and 54-281 μg N mhfor the two-,four-,and six-hour static chamber enclosures,respectively.The Nfluxes measured in 120 cases varied between 159 and 2 943(811 on average) μg N mh.which were higher than the detection limits,with the exception of only two cases.The Nfluxes at DAF 3 were significantly higher(by nearly 80%(P<0.01)) than those at DAF 1 and 5 in the maize experiment,while there were no significant differences among the irrigation times in the wheat experiment.The Nfluxes and the ratios of nitrous oxide(NO) to the NO plus Nfluxes following urea application to maize were approximately 65% and 11 times larger,respectively(P<0.01),than those following urea application to wheat.Such differences could be mainly attributed to the higher soil water contents,temperatures,and availability of soil N substrates in the maize experiment than in the wheat experiment.This study suggests that theNGF method is sensitive enough to measure in situ Nfluxes from intensively fertilized croplands with calcareous soils.展开更多
基金jointly supported by the National Natural Science Foundation of China(41877333,41830751 and 41761144054)the Basic Research Program of Frontier Sciences of Chinese Academy of Sciences(ZDBSLY-DQCOO7)the National Key Research and Development Program of China(2017YFD0200100)。
文摘Denitrification-induced nitrogen(N) losses from croplands may be greatly increased by intensive fertilization.However,the accurate quantification of these losses is still challenging due to insufficient available in situ measurements of soil dinitrogen(N) emissions.We carried out two one-week experiments in a maize-wheat cropping system with calcareous soil using theN gas-flux(NGF) method to measure in situ Nfluxes following urea application.Applications ofN-labeled urea(99 atom%,130-150 kg N ha) were followed by irrigation on the 1 st,3 rd,and 5 th days after fertilization(DAF 1,3,and 5,respectively).The detection limits of the soil Nfluxes were 163-1 565,81-485,and 54-281 μg N mhfor the two-,four-,and six-hour static chamber enclosures,respectively.The Nfluxes measured in 120 cases varied between 159 and 2 943(811 on average) μg N mh.which were higher than the detection limits,with the exception of only two cases.The Nfluxes at DAF 3 were significantly higher(by nearly 80%(P<0.01)) than those at DAF 1 and 5 in the maize experiment,while there were no significant differences among the irrigation times in the wheat experiment.The Nfluxes and the ratios of nitrous oxide(NO) to the NO plus Nfluxes following urea application to maize were approximately 65% and 11 times larger,respectively(P<0.01),than those following urea application to wheat.Such differences could be mainly attributed to the higher soil water contents,temperatures,and availability of soil N substrates in the maize experiment than in the wheat experiment.This study suggests that theNGF method is sensitive enough to measure in situ Nfluxes from intensively fertilized croplands with calcareous soils.
