Helicobacter pylori(H.pylori)infection is a threat to human health.The lipopolysaccharide(LPS)O-antigen holds promise for developing vaccines.It is meaningful to explore the immunological activity of oligosaccharides ...Helicobacter pylori(H.pylori)infection is a threat to human health.The lipopolysaccharide(LPS)O-antigen holds promise for developing vaccines.It is meaningful to explore the immunological activity of oligosaccharides with different lengths and frameshifts for antigen development.Herein,a glycan library of H.pylori O2 O-antigen containing eight fragments is constructed.After screening with anti-H.pylori O2 LPS sera and patients’sera by glycan microarray,the disaccharide HPO2G-2b and trisaccharide HPO2G-3a show strong antigenicity and then are separately conjugated with carrier protein CRM197.Both glycoconjugates elicit a robust immunoglobulin G(IgG)immune response in rabbits.The anti-HPO2G-3a IgG antibodies possess a much stronger binding affinity with the LPS and bacteria of H.pylori O2 than the anti-HPO2G-2b IgG antibodies.There is no cross-reaction between both sera IgG antibodies with LPS and bacteria of H.pylori O1,O6,and E.coli.The results demonstrate the trisaccharide HPO2G-3a is a promising candidate for H.pylori vaccine development.展开更多
Proteus species especially Proteus mirabilis and Proteus vulgaris are zoonotic pathogens which can cause public health disease.Owing to their antibiotic-resistance,developing vaccines against these pathogens is urgent...Proteus species especially Proteus mirabilis and Proteus vulgaris are zoonotic pathogens which can cause public health disease.Owing to their antibiotic-resistance,developing vaccines against these pathogens is urgently required.Herein,we describe the first synthesis of the common O-antigen of Proteus mirabilis OE and Proteus vulgaris TG 103.展开更多
腰果是引起人们过敏的主要食物之一。作者采用生物信息学方法,通过Pubmed网络服务器、生物信息分析软件SOPMA、swiss-model网络服务器、DNAStar生物分析软件等对腰果主要过敏原Ana o 2的结构和抗原表位进行预测,分析Ana o 2蛋白的抗原...腰果是引起人们过敏的主要食物之一。作者采用生物信息学方法,通过Pubmed网络服务器、生物信息分析软件SOPMA、swiss-model网络服务器、DNAStar生物分析软件等对腰果主要过敏原Ana o 2的结构和抗原表位进行预测,分析Ana o 2蛋白的抗原表位可能是108-111,181-186,217-218,234-238,244-255,283-287。这为腰果过敏原的进一步研究提供理论参考,并对开发低过敏腰果制品提供帮助。展开更多
基金financial support from the National Natural Science Foundation of China(22177041,22277042,22077052,22107037,22207042)the China Postdoctoral Science Foundation(2021M691279)+1 种基金the 111 Project(111-2-06)the Max Planck Society International Partner Group Program,and the China Scholarship Council(CSC)for funding.P.H.S.thanks the Max Plank Society forgenerous financial support.
文摘Helicobacter pylori(H.pylori)infection is a threat to human health.The lipopolysaccharide(LPS)O-antigen holds promise for developing vaccines.It is meaningful to explore the immunological activity of oligosaccharides with different lengths and frameshifts for antigen development.Herein,a glycan library of H.pylori O2 O-antigen containing eight fragments is constructed.After screening with anti-H.pylori O2 LPS sera and patients’sera by glycan microarray,the disaccharide HPO2G-2b and trisaccharide HPO2G-3a show strong antigenicity and then are separately conjugated with carrier protein CRM197.Both glycoconjugates elicit a robust immunoglobulin G(IgG)immune response in rabbits.The anti-HPO2G-3a IgG antibodies possess a much stronger binding affinity with the LPS and bacteria of H.pylori O2 than the anti-HPO2G-2b IgG antibodies.There is no cross-reaction between both sera IgG antibodies with LPS and bacteria of H.pylori O1,O6,and E.coli.The results demonstrate the trisaccharide HPO2G-3a is a promising candidate for H.pylori vaccine development.
基金This work was financially supported by the National Natural Science Foundation of China(22167015,22077052,22177041,21977039)Science and Technology Department of Jiangxi Province(jxsq2020101084).
文摘Proteus species especially Proteus mirabilis and Proteus vulgaris are zoonotic pathogens which can cause public health disease.Owing to their antibiotic-resistance,developing vaccines against these pathogens is urgently required.Herein,we describe the first synthesis of the common O-antigen of Proteus mirabilis OE and Proteus vulgaris TG 103.
基金国家自然科学基金项目(30871752,30570421)The International Science and Technology(ISAT)Linkages Fund,Royal Soci-ety of New Zealand(ISATB09-33)+1 种基金广东省科技重点专项(2003A3080502)教育部留学回国人员科研启动基金资助项目
文摘腰果是引起人们过敏的主要食物之一。作者采用生物信息学方法,通过Pubmed网络服务器、生物信息分析软件SOPMA、swiss-model网络服务器、DNAStar生物分析软件等对腰果主要过敏原Ana o 2的结构和抗原表位进行预测,分析Ana o 2蛋白的抗原表位可能是108-111,181-186,217-218,234-238,244-255,283-287。这为腰果过敏原的进一步研究提供理论参考,并对开发低过敏腰果制品提供帮助。
