目的:探讨高脂膳食诱导下,不同肥胖程度小鼠血清内源性大麻素(endocannabinoids,EC)、脂肪组织大麻素受体、合成酶和降解酶的变化趋势及其机制。方法:6周龄C57BL/6J雄性小鼠随机分为对照组(n=25,喂食普通饲料)和高脂诱导组(n=205,喂食D1...目的:探讨高脂膳食诱导下,不同肥胖程度小鼠血清内源性大麻素(endocannabinoids,EC)、脂肪组织大麻素受体、合成酶和降解酶的变化趋势及其机制。方法:6周龄C57BL/6J雄性小鼠随机分为对照组(n=25,喂食普通饲料)和高脂诱导组(n=205,喂食D12492高脂饲料,脂肪提供热量占60%),诱导时间10周。对照组随机挑选10只平均体重记为X(g);高脂诱导组按体重分类标准分4组(每组随机挑选10只):抵抗组(X×0.9<体重≤X×1.1)、超重组(X×1.1<体重≤X×1.2)、肥胖组(X×1.2<体重≤X×1.5)和严重肥胖组(体重>X×1.5)。采用高效液相色谱法检测血清大麻素含量,荧光定量PCR检测脂肪组织大麻素受体、合成酶和降解酶表达变化。结果:1)从对照组、超重组、肥胖组到严重肥胖组,血清大麻素平均水平依次升高,但只有严重肥胖组血清2-AG、AEA和OEA与对照组相比显著升高(P<0.01,P<0.05和P<0.05)。2)(超重+肥胖+严重肥胖组)VS对照组,脂肪组织大麻素受体CB1,CB2 m NRA表达下降(P<0.05和P<0.05),2-AG合成酶DAGLβ表达上升(P<0.01)。3)将超重、肥胖和严重肥胖组小鼠综合起来分析发现,血清2-AG与体重(P=0.004,r=0.586)和内脏脂肪量(P=0.001,r=0.647)均呈中度正相关,而血清AEA仅与内脏脂肪量呈正相关(P=0.034,r=0.453)。4)内脏脂肪量与脂肪组织2-AG合成酶ABHD4(P=0.022,r=-0.466)、DAGLβ(P=0.019,r=-0.475)、降解酶ABHD6(P=0.000,r=-0.738)、MAGL(P=0.012,r=-0.505)、AEA和OEA的共同降解酶NAAA(P=0.003,r=-0.586)m RNA表达呈负相关。结论:高脂诱导下,超重、肥胖和严重肥胖组小鼠血清循环大麻素水平依次升高,脂肪组织大麻素受体CB1表达依次下降,大麻素合成酶与降解酶与内脏脂肪量均呈负相关。因此我们推测:对于相同诱导时间下的不同肥胖程度小鼠,大麻素合成酶表达下调的速度低于降解酶和CB1受体的下调速度,使得循环大麻素水平升高,这些因素共同促成了肥胖相关的外周ECS活化。展开更多
Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quant...Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from rodent brain tissue. Analytes were exwacted using acetonitfile without further sample clean up, resolved on a C 18 reverse-phase column using a gradient mobile phase and detected using eleclmspmy ionization in positive selected ion monitoring mode on a single quadrupole mass spectrometer. The method produced high recovery rates for AEA, OEA and PEA, ranging from 98.1% to 106.2%, 98.5% to 102.2% and 85.4% to 89.5%, respectively. The method resulted in adequate sensitivity with a lower limit of quantification for AEA, OEA展开更多
A spinel oxide NiCo204 prepared by thermal decomposition is of very high activity for the oxygen evolution reaction(OER)in alkaline solution.The oxygen evolution overpotential on NiCo204 is 0.252-0.262V in 10 M NaOH s...A spinel oxide NiCo204 prepared by thermal decomposition is of very high activity for the oxygen evolution reaction(OER)in alkaline solution.The oxygen evolution overpotential on NiCo204 is 0.252-0.262V in 10 M NaOH solution at 343K and current density 100 mAcm^(-2).展开更多
Triheptyl cellulose/ethyl cellulose(3/97)binary blend membranes were prepared from tetrahydrofuran,chloroform and dichloromethane solutions and their air separation capabit- ities were studied at different temperature...Triheptyl cellulose/ethyl cellulose(3/97)binary blend membranes were prepared from tetrahydrofuran,chloroform and dichloromethane solutions and their air separation capabit- ities were studied at different temperatures.With increasing temperature from 25 to 85℃,the flux QOEA of O_2-enriched air(OEA),O_2 permselectivity and the O_2 concentration Yo_2 in the OEA all increase.The membranes show a unique trend in their Yo_2～QOEA relationship,that is,the air separation capability increases simultaneously with the OEA permeation capability.The magnitudes of QOEA and Yo_2 for 17μm-thick membrane after the testg time of 36hours at 70℃ are 5×10^(-4)cm^3 (STP)/s·cm^2 and 37.6%,respectively.The air separation capability depends slightly on membrane forming solvents.展开更多
文摘目的:探讨高脂膳食诱导下,不同肥胖程度小鼠血清内源性大麻素(endocannabinoids,EC)、脂肪组织大麻素受体、合成酶和降解酶的变化趋势及其机制。方法:6周龄C57BL/6J雄性小鼠随机分为对照组(n=25,喂食普通饲料)和高脂诱导组(n=205,喂食D12492高脂饲料,脂肪提供热量占60%),诱导时间10周。对照组随机挑选10只平均体重记为X(g);高脂诱导组按体重分类标准分4组(每组随机挑选10只):抵抗组(X×0.9<体重≤X×1.1)、超重组(X×1.1<体重≤X×1.2)、肥胖组(X×1.2<体重≤X×1.5)和严重肥胖组(体重>X×1.5)。采用高效液相色谱法检测血清大麻素含量,荧光定量PCR检测脂肪组织大麻素受体、合成酶和降解酶表达变化。结果:1)从对照组、超重组、肥胖组到严重肥胖组,血清大麻素平均水平依次升高,但只有严重肥胖组血清2-AG、AEA和OEA与对照组相比显著升高(P<0.01,P<0.05和P<0.05)。2)(超重+肥胖+严重肥胖组)VS对照组,脂肪组织大麻素受体CB1,CB2 m NRA表达下降(P<0.05和P<0.05),2-AG合成酶DAGLβ表达上升(P<0.01)。3)将超重、肥胖和严重肥胖组小鼠综合起来分析发现,血清2-AG与体重(P=0.004,r=0.586)和内脏脂肪量(P=0.001,r=0.647)均呈中度正相关,而血清AEA仅与内脏脂肪量呈正相关(P=0.034,r=0.453)。4)内脏脂肪量与脂肪组织2-AG合成酶ABHD4(P=0.022,r=-0.466)、DAGLβ(P=0.019,r=-0.475)、降解酶ABHD6(P=0.000,r=-0.738)、MAGL(P=0.012,r=-0.505)、AEA和OEA的共同降解酶NAAA(P=0.003,r=-0.586)m RNA表达呈负相关。结论:高脂诱导下,超重、肥胖和严重肥胖组小鼠血清循环大麻素水平依次升高,脂肪组织大麻素受体CB1表达依次下降,大麻素合成酶与降解酶与内脏脂肪量均呈负相关。因此我们推测:对于相同诱导时间下的不同肥胖程度小鼠,大麻素合成酶表达下调的速度低于降解酶和CB1受体的下调速度,使得循环大麻素水平升高,这些因素共同促成了肥胖相关的外周ECS活化。
基金supported by funding from the National Institute ofAlcohol Abuse and Alcoholism AA016959(KN),AA016499(KN)and AA019853(DJL)and the Kentucky Science andTechnology Corporation(KSTC-184-512-07-029ALS)
文摘Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from rodent brain tissue. Analytes were exwacted using acetonitfile without further sample clean up, resolved on a C 18 reverse-phase column using a gradient mobile phase and detected using eleclmspmy ionization in positive selected ion monitoring mode on a single quadrupole mass spectrometer. The method produced high recovery rates for AEA, OEA and PEA, ranging from 98.1% to 106.2%, 98.5% to 102.2% and 85.4% to 89.5%, respectively. The method resulted in adequate sensitivity with a lower limit of quantification for AEA, OEA
文摘A spinel oxide NiCo204 prepared by thermal decomposition is of very high activity for the oxygen evolution reaction(OER)in alkaline solution.The oxygen evolution overpotential on NiCo204 is 0.252-0.262V in 10 M NaOH solution at 343K and current density 100 mAcm^(-2).
文摘Triheptyl cellulose/ethyl cellulose(3/97)binary blend membranes were prepared from tetrahydrofuran,chloroform and dichloromethane solutions and their air separation capabit- ities were studied at different temperatures.With increasing temperature from 25 to 85℃,the flux QOEA of O_2-enriched air(OEA),O_2 permselectivity and the O_2 concentration Yo_2 in the OEA all increase.The membranes show a unique trend in their Yo_2～QOEA relationship,that is,the air separation capability increases simultaneously with the OEA permeation capability.The magnitudes of QOEA and Yo_2 for 17μm-thick membrane after the testg time of 36hours at 70℃ are 5×10^(-4)cm^3 (STP)/s·cm^2 and 37.6%,respectively.The air separation capability depends slightly on membrane forming solvents.
