[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The...[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.展开更多
mRNA基因芯片表达谱为头颈肿瘤(head and neck cancer,HNC)的分子生物学诊断及研究HNC的生物过程提供了诸多重要生物学信息。通过生物信息学技术研究HNC基因表达谱,从而发掘头颈癌新的分子标志物。对4个GSE数据集和癌症基因组图谱(cance...mRNA基因芯片表达谱为头颈肿瘤(head and neck cancer,HNC)的分子生物学诊断及研究HNC的生物过程提供了诸多重要生物学信息。通过生物信息学技术研究HNC基因表达谱,从而发掘头颈癌新的分子标志物。对4个GSE数据集和癌症基因组图谱(cancer genome atlas,TCGA)头颈癌数据库进行了综合分析,鉴定出HNC和相邻正常组织(adjacent normal tissue,ANT)样本中3 639个差异表达基因(differentially expressed genes,DEGs),然后应用加权基因共表达网络分析相关差异表达基因DEGs和HNC临床特征之间的关系。鉴定出15个共表达基因模块。其中蓝绿色基因模块所对应HNC患者的风险比(hazard ratio,HR)最高。通过对该模块的进一步研究,发现氧化低密度脂蛋白1(oxidized low density lipoprotein receptor 1,OLR1)的表达量与HNC患者的总体生存率呈显著负相关性。OLR1在mRNA水平的表达水平可作为判断HNC患者生存预后的潜在分子标志物。展开更多
基金Supported by Key Project in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2008BADB2B04-8-4)Natural Science Foundation of Hebei Province(No.C2008001308 and C2007000743)~~
文摘[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.
