针对目前EPCglobal ONS(Object Name Service)系统存在的信息容易被偷听、篡改、欺骗等威胁,提出一种基于DNSCurve协议和椭圆曲线加密机制的ONS系统来实现产品动态信息在查询过程中的安全性和隐私性,并通过实验验证此系统的安全性能,能...针对目前EPCglobal ONS(Object Name Service)系统存在的信息容易被偷听、篡改、欺骗等威胁,提出一种基于DNSCurve协议和椭圆曲线加密机制的ONS系统来实现产品动态信息在查询过程中的安全性和隐私性,并通过实验验证此系统的安全性能,能够抵御偷听、篡改、欺骗等攻击,有效地加强了ONS系统安全,对整个物联网的发展具有很重要的现实意义。展开更多
AIM: To investigate whether whole-exome sequencing may serve as an efficient method to identify known or novel colorectal cancer(CRC) predisposing genes in early-onset or familial CRC cases.METHODS: We performed whole...AIM: To investigate whether whole-exome sequencing may serve as an efficient method to identify known or novel colorectal cancer(CRC) predisposing genes in early-onset or familial CRC cases.METHODS: We performed whole-exome sequencing in 23 Chinese patients from 21 families with nonpolyposis CRC diagnosed at ≤ 40 years of age, or from multiple affected CRC families with at least 1 firstdegree relative diagnosed with CRC at ≤ 55 years of age.Genomic DNA from blood was enriched for exome sequences using the Sure Select Human All Exon Kit, version 2(Agilent Technologies) and sequencing was performed on an Illumina Hi Seq 2000 platform.Data were processed through an analytical pipeline to search for rare germline variants in known or novel CRC predisposing genes.RESULTS: In total, 32 germline variants in 23 genes were identified and confirmed by Sanger sequencing.In 6 of the 21 families(29%), we identified 7 mutations in 3 known CRC predisposing genes including MLH1(5 patients), MSH2(1 patient), and MUTYH(biallelic, 1 patient), five of which were reported as pathogenic.Inthe remaining 15 families, we identified 20 rare and novel potentially deleterious variants in 19 genes, six of which were truncating mutations.One previously unreported variant identified in a conserved region of EIF2AK4(p.Glu738_Asp739insA rgA rg) was found to represent a local Chinese variant, which was significantly enriched in our early-onset CRC patient cohort compared to a control cohort of 100 healthy Chinese individuals scored negative by colonoscopy(33.3% vs 7%, P < 0.001).CONCLUSION: Whole-exome sequencing of early-onset or familial CRC cases serves as an efficient method to identify known and potential pathogenic variants in established and novel candidate CRC predisposing genes.展开更多
Transition metal sulfides (TMSs) have a wide range of applications owing to their intriguing properties.Significant efforts have been devoted to nanostructuring TMSs to enhance their properties and performance,still t...Transition metal sulfides (TMSs) have a wide range of applications owing to their intriguing properties.Significant efforts have been devoted to nanostructuring TMSs to enhance their properties and performance,still there is a high need in general synthesis of TMS nanostructures.Herein,for the first time,a simple solvent free reactive nanocasting approach that integrates solid precursor loading,in-situ sulfuration and carbonization into a single heating step is developed for the universal synthesis of ordered mesoporous TMS@N-doped carbon composites (denoted as OM-TMS@NCs) with methionine (Met) and metal chlorides as the precursors and the mesoporous silica (SBA-15) as the hard template.A series of OM-TMS@NCs with a hexagonal mesostructure,ultra-high surface areas (430-754 m2·g-1),large pore volumes (0.85-1.32 cm3·g-1),and unique TMS stoichiometries,including MoS2,Fe7S8,Co9S8,NiS,Cu7S4 and ZnS,are obtained.Two distinct structure configurations,namely,highly dispersed ultrathin TMS nanosheets within NCs and TMS@NC co-nanowire arrays,can be obtained depending on different metals.The structure evolution of the OM-TMS@NCs over the solvent-free nanocasting process is studied in detail for a deep understanding of the synthesis.As demonstrations,these materials are promising for electrocatalytic hydrogen evolution reaction and lithium ion storage with high performances.展开更多
Efficie nt in teracti on betwee n art and tech no logy en abled Islamic architecture to actively experience early globalization, but today's separation between these domains and technological hegemony have present...Efficie nt in teracti on betwee n art and tech no logy en abled Islamic architecture to actively experience early globalization, but today's separation between these domains and technological hegemony have presented challenges to Islamic architecture. Accordingly, this research was aimed at illuminating art and technology interactions in Islamic architecture during early globalization when it flourished along with Christian architecture. To this end, logical reasoning and qualitative data an alysis were performed in parallel on 12 chai ns of mostly worshipone nted artworks, such as mosques and churches. The results indicated that art and tech no logy interactions in Islamic and Christian architecture were underlain by the same nature and seque ntial pattern of begi nning from primitive tech no logy and endi ng with inn ovative technology (art). The cultural and technological value of these architectural styles eventually crossed geographical boundaries. Therefore, the beneficial interactions between art and technology enabled Islamic and Christian architecture to participate in globalization through the con veya nee of their value to other regi ons eve n as d if fere nces existed in the measures implemented for such transmission.展开更多
文摘针对目前EPCglobal ONS(Object Name Service)系统存在的信息容易被偷听、篡改、欺骗等威胁,提出一种基于DNSCurve协议和椭圆曲线加密机制的ONS系统来实现产品动态信息在查询过程中的安全性和隐私性,并通过实验验证此系统的安全性能,能够抵御偷听、篡改、欺骗等攻击,有效地加强了ONS系统安全,对整个物联网的发展具有很重要的现实意义。
基金Supported by research grants from the Dutch Cancer Society(KWF,KUN-4335)the Netherlands Organization for Scientific Research(NWO,91710358)+2 种基金the Royal Dutch Academy of Sciences(KNAW)National Natural Science Foundation of China(NSFC,81272194 and 81072041)a scholarship from the China Scholarship Council(CSC)to Zhang JX
文摘AIM: To investigate whether whole-exome sequencing may serve as an efficient method to identify known or novel colorectal cancer(CRC) predisposing genes in early-onset or familial CRC cases.METHODS: We performed whole-exome sequencing in 23 Chinese patients from 21 families with nonpolyposis CRC diagnosed at ≤ 40 years of age, or from multiple affected CRC families with at least 1 firstdegree relative diagnosed with CRC at ≤ 55 years of age.Genomic DNA from blood was enriched for exome sequences using the Sure Select Human All Exon Kit, version 2(Agilent Technologies) and sequencing was performed on an Illumina Hi Seq 2000 platform.Data were processed through an analytical pipeline to search for rare germline variants in known or novel CRC predisposing genes.RESULTS: In total, 32 germline variants in 23 genes were identified and confirmed by Sanger sequencing.In 6 of the 21 families(29%), we identified 7 mutations in 3 known CRC predisposing genes including MLH1(5 patients), MSH2(1 patient), and MUTYH(biallelic, 1 patient), five of which were reported as pathogenic.Inthe remaining 15 families, we identified 20 rare and novel potentially deleterious variants in 19 genes, six of which were truncating mutations.One previously unreported variant identified in a conserved region of EIF2AK4(p.Glu738_Asp739insA rgA rg) was found to represent a local Chinese variant, which was significantly enriched in our early-onset CRC patient cohort compared to a control cohort of 100 healthy Chinese individuals scored negative by colonoscopy(33.3% vs 7%, P < 0.001).CONCLUSION: Whole-exome sequencing of early-onset or familial CRC cases serves as an efficient method to identify known and potential pathogenic variants in established and novel candidate CRC predisposing genes.
基金Financial supports from the National Natural Science Foundation of China(Nos.21875153 and 21501125)the Natural Science Foundation of Jiangsu Province(No.BK20150312)+1 种基金the Suzhou Bureau of Science and Technology(No.SYG201637)are much appreciatedWe also thank the Young Thousand Talented Program(2015)of China,the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions and the Project of Scientific and Technologic Infrastructure of Suzhou(No.SZS201708)for supports.
文摘Transition metal sulfides (TMSs) have a wide range of applications owing to their intriguing properties.Significant efforts have been devoted to nanostructuring TMSs to enhance their properties and performance,still there is a high need in general synthesis of TMS nanostructures.Herein,for the first time,a simple solvent free reactive nanocasting approach that integrates solid precursor loading,in-situ sulfuration and carbonization into a single heating step is developed for the universal synthesis of ordered mesoporous TMS@N-doped carbon composites (denoted as OM-TMS@NCs) with methionine (Met) and metal chlorides as the precursors and the mesoporous silica (SBA-15) as the hard template.A series of OM-TMS@NCs with a hexagonal mesostructure,ultra-high surface areas (430-754 m2·g-1),large pore volumes (0.85-1.32 cm3·g-1),and unique TMS stoichiometries,including MoS2,Fe7S8,Co9S8,NiS,Cu7S4 and ZnS,are obtained.Two distinct structure configurations,namely,highly dispersed ultrathin TMS nanosheets within NCs and TMS@NC co-nanowire arrays,can be obtained depending on different metals.The structure evolution of the OM-TMS@NCs over the solvent-free nanocasting process is studied in detail for a deep understanding of the synthesis.As demonstrations,these materials are promising for electrocatalytic hydrogen evolution reaction and lithium ion storage with high performances.
文摘Efficie nt in teracti on betwee n art and tech no logy en abled Islamic architecture to actively experience early globalization, but today's separation between these domains and technological hegemony have presented challenges to Islamic architecture. Accordingly, this research was aimed at illuminating art and technology interactions in Islamic architecture during early globalization when it flourished along with Christian architecture. To this end, logical reasoning and qualitative data an alysis were performed in parallel on 12 chai ns of mostly worshipone nted artworks, such as mosques and churches. The results indicated that art and tech no logy interactions in Islamic and Christian architecture were underlain by the same nature and seque ntial pattern of begi nning from primitive tech no logy and endi ng with inn ovative technology (art). The cultural and technological value of these architectural styles eventually crossed geographical boundaries. Therefore, the beneficial interactions between art and technology enabled Islamic and Christian architecture to participate in globalization through the con veya nee of their value to other regi ons eve n as d if fere nces existed in the measures implemented for such transmission.
