Background: Nowadays, emergence of Carbapenemase-Producing Enterobacterales (CPE) throughout the world has become a public health problem, especially in countries with limited resources. In recent years, CPE of type O...Background: Nowadays, emergence of Carbapenemase-Producing Enterobacterales (CPE) throughout the world has become a public health problem, especially in countries with limited resources. In recent years, CPE of type OXA-48 (Ambler class D) have been identified in Dakar. The aim of this study was to evaluate the phenotypic detection of OXA-48 CPE using a temocillin disc (30 μg). Methodology: A retrospective study was carried out at Medical Biology Laboratory of Pasteur Institute in Dakar on Ertapenem-Resistant Enterobacterales (ERE) strains isolated from 2015 to 2017. These strains were then tested with a 30 μg temocillin disc. Any strain resistant to temocillin (inhibition diameter Results: Forty-one ERE isolated during the study period were tested, of which 34 (82.9%) were OXA-48 based on phenotypic detection using temocillin disc and confirmed by PCR (100%). OXA-48 CPE strains detected were composed of Klebsiella pneumoniae (n = 14;41.2%), Enterobacter cloacae (n = 8;23.5%), Escherichia coli (n = 7, 20.5%), Citrobacter freundii (n = 3;8.8%), Cronobacter sakazakii (n = 1;3%) and Morganella morganii (n = 1;3%). Conclusion: Temocillin resistance has a good positive predictive value for detecting OXA-48 CPE by phenotypic method, confirmed by PCR. Temocillin is therefore a good marker for detection of OXA-48 CPE except Hafnia alvei.展开更多
目的研究复方五味子素B对耐奥沙利铂人结肠癌细胞(TH C-8307/O X A)多药耐药性的逆转作用及其机制。方法采用噻唑蓝(M TT)比色法检测奥沙利铂(O X A)和复方五味子素B(γSC)的细胞毒性;碱性磷酸酶免疫组织化学法和W estern blot检测γSC...目的研究复方五味子素B对耐奥沙利铂人结肠癌细胞(TH C-8307/O X A)多药耐药性的逆转作用及其机制。方法采用噻唑蓝(M TT)比色法检测奥沙利铂(O X A)和复方五味子素B(γSC)的细胞毒性;碱性磷酸酶免疫组织化学法和W estern blot检测γSC对TH C-8307/O X A细胞G ST-п蛋白水平的影响。结果γSC(6.0、12.5和25.0μg/m L)对人结肠癌细胞(TH C-8307)和耐奥沙利铂人结肠癌细胞(TH C-8307/O XA)无显著毒性作用(P>0.05),O X A对TH C-8307的IC 50为0.06μg/m L,而对TH C-8307/O X A的IC 50为2.32μg/m L,TH C-8307/O X A是TH C-8307对O X A耐药的39倍,γSC(6.0、12.5和25.0μg/m L)能使O X A对TH C-8307/O X A细胞的IC 50从2.32μg/m L依次下降至0.370、0.128和0.057μg/m L,逆转倍数分别为6.2、18.1和40.7倍。碱性磷酸酶免疫组织化学法和W estern blot检测γSC(12.5μg/m L)处理48 h后,TH C-8307/O X A细胞G ST-п蛋白表达明显降低。结论γSC具有逆转耐奥沙利铂人结肠癌细胞的M D R作用,其作用机制与下调G ST-п表达有关。展开更多
文摘Background: Nowadays, emergence of Carbapenemase-Producing Enterobacterales (CPE) throughout the world has become a public health problem, especially in countries with limited resources. In recent years, CPE of type OXA-48 (Ambler class D) have been identified in Dakar. The aim of this study was to evaluate the phenotypic detection of OXA-48 CPE using a temocillin disc (30 μg). Methodology: A retrospective study was carried out at Medical Biology Laboratory of Pasteur Institute in Dakar on Ertapenem-Resistant Enterobacterales (ERE) strains isolated from 2015 to 2017. These strains were then tested with a 30 μg temocillin disc. Any strain resistant to temocillin (inhibition diameter Results: Forty-one ERE isolated during the study period were tested, of which 34 (82.9%) were OXA-48 based on phenotypic detection using temocillin disc and confirmed by PCR (100%). OXA-48 CPE strains detected were composed of Klebsiella pneumoniae (n = 14;41.2%), Enterobacter cloacae (n = 8;23.5%), Escherichia coli (n = 7, 20.5%), Citrobacter freundii (n = 3;8.8%), Cronobacter sakazakii (n = 1;3%) and Morganella morganii (n = 1;3%). Conclusion: Temocillin resistance has a good positive predictive value for detecting OXA-48 CPE by phenotypic method, confirmed by PCR. Temocillin is therefore a good marker for detection of OXA-48 CPE except Hafnia alvei.
文摘目的研究复方五味子素B对耐奥沙利铂人结肠癌细胞(TH C-8307/O X A)多药耐药性的逆转作用及其机制。方法采用噻唑蓝(M TT)比色法检测奥沙利铂(O X A)和复方五味子素B(γSC)的细胞毒性;碱性磷酸酶免疫组织化学法和W estern blot检测γSC对TH C-8307/O X A细胞G ST-п蛋白水平的影响。结果γSC(6.0、12.5和25.0μg/m L)对人结肠癌细胞(TH C-8307)和耐奥沙利铂人结肠癌细胞(TH C-8307/O XA)无显著毒性作用(P>0.05),O X A对TH C-8307的IC 50为0.06μg/m L,而对TH C-8307/O X A的IC 50为2.32μg/m L,TH C-8307/O X A是TH C-8307对O X A耐药的39倍,γSC(6.0、12.5和25.0μg/m L)能使O X A对TH C-8307/O X A细胞的IC 50从2.32μg/m L依次下降至0.370、0.128和0.057μg/m L,逆转倍数分别为6.2、18.1和40.7倍。碱性磷酸酶免疫组织化学法和W estern blot检测γSC(12.5μg/m L)处理48 h后,TH C-8307/O X A细胞G ST-п蛋白表达明显降低。结论γSC具有逆转耐奥沙利铂人结肠癌细胞的M D R作用,其作用机制与下调G ST-п表达有关。
