Petroleum refinery wastewater （PRW） containing hydrocarbon is highly toxic to the environment and the surrounding ecosystem. Proper treatment of the PRW effluent is necessary to remove the pollutants before discharg...Petroleum refinery wastewater （PRW） containing hydrocarbon is highly toxic to the environment and the surrounding ecosystem. Proper treatment of the PRW effluent is necessary to remove the pollutants before discharge. Bioremediation is considered to be a promising approach as it is eco- friendly and efficient. The exopolysaccharide （EPS） produced by the O. anthropi acts as a bioemulsifier and showed the highest emulsification activity of 60% on diesel. An EPS yield of about 0.42 g/L was obtained under optimized conditions. The carbohydrate and protein content of the EPS was found to be 71.1% and 19.7% respectively, showing the glycoprotein nature. The structural properties of EPS were analyzed by FT-IR and 1H NMR. The batch degradation of oil in PRW by O. anthropi was studied gravimetrically, and showed about 53% degradation in 7 days, indicating the strong ability of the isolated strain to degrade the hydrocarbons in PRW.展开更多
One Gram-negative Bacillus was isolated from a brain sample of a pig with neurological symptoms.Pathological examination showed meningitis at necropsy. Ochrobactrum anthropi (O. anthropi) was successfully isolated fro...One Gram-negative Bacillus was isolated from a brain sample of a pig with neurological symptoms.Pathological examination showed meningitis at necropsy. Ochrobactrum anthropi (O. anthropi) was successfully isolated from the brain sample and was confirmed by biochemical reaction results (API 20 NE) and gene sequencing. The strain was highly resistant to b-lactam antibiotics. Mice were experimentally infected with O. anthropi and showed typical meningitis. This is the first report on O. anthropi isolated from a pig, and indicates that O. anthropi may have a broader host spectrum of infection.展开更多
A Gram-negative, chromium（Ⅵ） tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis...A Gram-negative, chromium（Ⅵ） tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis. It was able to tolerate up to 10 mmol/L Cr（Ⅵ） and completely reduce 1 mmol/L Cr（Ⅵ） to Cr（Ⅲ） within 48 h. When the strain CTS-325 was induced with Cr（Ⅵ）, a protein increased significantly in the whole cell proteins. Liquid chromatography tandem mass spectrometry （LC-MS/MS） analysis revealed that this protein was a superoxide dismutase （SOD） homology. The measured superoxide dismutase activity was 2694 U/mg after three steps of purification. The SOD catalyzes the dismutation of the superoxide anion （O2^←） into hydrogen peroxide and molecular oxygen. This protein is considered to be one of the most important anti-oxidative enzymes for O. anthropi as it allows the bacterium to survive high oxygen stress environments, such as the environment produced during the reduction process of Cr（Ⅵ）.展开更多
Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete dur...Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar（sucrose residue leaching solution） well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations.展开更多
基金the Department of Science and Technology, India for financial support under fast track scheme for young scientist (SR/FT/LS-19/2012)
文摘Petroleum refinery wastewater （PRW） containing hydrocarbon is highly toxic to the environment and the surrounding ecosystem. Proper treatment of the PRW effluent is necessary to remove the pollutants before discharge. Bioremediation is considered to be a promising approach as it is eco- friendly and efficient. The exopolysaccharide （EPS） produced by the O. anthropi acts as a bioemulsifier and showed the highest emulsification activity of 60% on diesel. An EPS yield of about 0.42 g/L was obtained under optimized conditions. The carbohydrate and protein content of the EPS was found to be 71.1% and 19.7% respectively, showing the glycoprotein nature. The structural properties of EPS were analyzed by FT-IR and 1H NMR. The batch degradation of oil in PRW by O. anthropi was studied gravimetrically, and showed about 53% degradation in 7 days, indicating the strong ability of the isolated strain to degrade the hydrocarbons in PRW.
文摘One Gram-negative Bacillus was isolated from a brain sample of a pig with neurological symptoms.Pathological examination showed meningitis at necropsy. Ochrobactrum anthropi (O. anthropi) was successfully isolated from the brain sample and was confirmed by biochemical reaction results (API 20 NE) and gene sequencing. The strain was highly resistant to b-lactam antibiotics. Mice were experimentally infected with O. anthropi and showed typical meningitis. This is the first report on O. anthropi isolated from a pig, and indicates that O. anthropi may have a broader host spectrum of infection.
基金supported by the National Basic Research Program (973) of China (No. 2007CB815601)the National Natural Science Foundation of China (No.40902097, 40772034, 10776027)+1 种基金the Special Foundation of the President of the Chinese Academy of Sciencesthe Opening Project of Key Laboratory of Solid Waste Treatment and Resource Recycle (No. 09ZXGK05),Ministry of Education
文摘A Gram-negative, chromium（Ⅵ） tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis. It was able to tolerate up to 10 mmol/L Cr（Ⅵ） and completely reduce 1 mmol/L Cr（Ⅵ） to Cr（Ⅲ） within 48 h. When the strain CTS-325 was induced with Cr（Ⅵ）, a protein increased significantly in the whole cell proteins. Liquid chromatography tandem mass spectrometry （LC-MS/MS） analysis revealed that this protein was a superoxide dismutase （SOD） homology. The measured superoxide dismutase activity was 2694 U/mg after three steps of purification. The SOD catalyzes the dismutation of the superoxide anion （O2^←） into hydrogen peroxide and molecular oxygen. This protein is considered to be one of the most important anti-oxidative enzymes for O. anthropi as it allows the bacterium to survive high oxygen stress environments, such as the environment produced during the reduction process of Cr（Ⅵ）.
基金Supported by the National Basic Research Program of China (973 program,No. 2007CB815601,2010CB933501)the National Natural Science Foundation of China (40772034, 40902097)+2 种基金 the Outstanding Youth Fund (50625205) the Opening Project of Key Laboratory of Solid Waste Treatment and Resource Recycle (No. 09zxgk05),Ministry of Educationthe CAS Foundation (KJCX1.YW.07)
文摘Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar（sucrose residue leaching solution） well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations.
