Flame-retardant oligomeric electrolyte additive for self-extinguishing and highly-stable lithium-ion batteries:Beyond small molecules

Preparing both safe and high-performance lithium-ion batteries(LIBs) based on commonly used commercial electrolytes is highly desirable,yet challenging.To overcome the poor compatibility of conventional small-molecular flame-retardants as electrolyte additives for safe LIBs with graphite anodes,in this study,we propose and design a novel low-cost flame-retardant oligomer that achieves an accurate and complete reconciliation of fire safety and electrochemical performance in LIBs.Owing to the integration of phosphonate units and polyethylene glycol(PEG) chains,this oligomer,which is a phosphonatecontaining PEG-based oligomer(PPO),not only endows commercial electrolytes with excellent flame retardancy but also helps stabilize the electrodes and Li-ion migration.Specifically,adding 15 wt% of PPO can reduce 70% of the self-extinguishing time and 54% of total heat release for commercial electrolytes.Moreover,LiFePO_(4)/lithium and graphite/lithium cells as well as LiFePO_(4)/graphite pouch full cells exhibit good long-term cycling stability.

Human islet amyloid polypeptide oligomers stabilized and probed by MAS NMR

The capture and characterization of oligomers are extremely important in the studies of amyloid aggregation of proteins and peptides.Oligomers are critical intermediates that can impact the structures of amyloid fibrils.Moreover,it is widely accepted that oligomers are the most toxic species along the aggregation pathway[1e4].The studies of oligomers are believed to shed light on the molecular mechanism of amyloid fibrillation and probably the medical clues for related diseases.In vitro investigations of amyloid oligomers are challenging due to their transient and polymorphic nature[5].This is particularly evident in the case of human type-2 diabetes-associated islet amyloid polypeptide(hIAPP),which tends to rapidly form polymorphic fibrils within minutes[6].Notably,hIAPP demonstrates a higher propensity for rapid aggregation compared to other amyloid proteins such as a-synuclein[7].

Association between serum cartilage oligomeric matrix protein and coronary artery calcification in maintenance hemodialysis patients

Background Coronary artery calcification(CAC)is common in end-stage renal disease(ESRD)patients,and the extent of CAC is closely related to cardiovascular outcomes in ESRD patients.Cartilage oligomeric matrix protein(COMP),as a component of the vascular matrix,has been found to be an inhibitor of arterial calcification in basic studies.However,there is no clinical research on the correlation between COMP and CAC in maintenance hemodialysis(MHD)patients.The aim of this study was to explore the relationship between serum COMP levels and CAC and cardiovascular events in MHD patients.Methods Serum COMP levels were compared between 54 MHD patients and 66 healthy people.MHD patients were then divided into three groups according to the tertiles of the concentration of COMP level and were followed up for major adverse cardiac events(MACEs),which were defined as a combined end point of new onset angina pectoris,nonfatal myocardial infarction,heart failure,coronary artery revascularization,hospitalization due to angina pectoris and all-cause deaths.The CAC score was calculated based on computed tomography scans.Results The serum COMP level in MHD patients was significantly higher than that in the general population[984.23(248.43-1902.61)ng/mL vs.219.01(97.26-821.92)ng/mL,P<0.01].Serum COMP levels were positively correlated with CAC(r=0.313,P=0.021)and serum parathyroid hormone in MHD patients(r=0.359,P<0.01).Linear regression suggested that after adjusting for age,fasting blood glucose(Glu)and glycosylated hemoglobin(HbAlc),CAC score was an independent predictor in the final model for COMP level(β=0.424,t=3.130,P<0.01).The receiver operating characteristic(ROC)curve showed that COMP≥994 mg/mL had 68.0%sensitivity and 72.4%specificity for the prediction of severe CAC[area under the curve(AUC):0.674,P=0.030,95%CI:0.526-0.882].After a median follow-up of 16 months(8-24 months),there was no difference in the incidence rate of MACEs between the upper,middle and lower serum COMP groups.Conclusions Our study found that MHD patients have higher levels of circulating COMP than controls.The serum COMP level is positively correlated with CAC score and could be used as a biomarker of severe CAC in MHD patients.However,there is no obvious correlation between serum COMP levels and the incidence of cardiovascular events.

Oligomeric proanthocyanidin protects retinal ganglion cells against oxidative stress-induced apoptosis

The death of retinal ganglion cells is a hallmark of many optic neurodegenerative diseases such as glaucoma and retinopathy. Oxidative stress is one of the major reasons to cause the cell death. Oligomeric proanthocyanidin has many health beneficial effects including antioxidative and neuro- protective actions. Here we tested whether oligomeric proanthocyanidin may protect retinal gan- glion cells against oxidative stress induced-apoptosis in vitro. Retinal ganglion cells were treated with hydrogen peroxide with or without oligomeric proanthocyanidin. 3-（4,5-Dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay showed that treating retinal ganglion cell line RGC-5 cells with 20 pmol/L oligomeric proanthocyanidin significantly decreased the hydrogen peroxide （H202） induced death. Results of flow cytometry and Hoechst staining demonstrated that the death of RGC-5 cells was mainly caused by cell apoptosis. We further found that expression of pro-apoptotic Bax and caspase-3 were significantly decreased while anti-apoptotic Bcl-2 was greatly increased in H202 damaged RGC-5 cells with oligomeric proanthocyanidin by western blot assay. Furthermore, in retinal explant culture, the number of surviving retinal ganglion cells in H202-damaged retinal ganglion cells with oligomeric proanthocyanidin was significantly increased. Our studies thus demonstrate that oligomeric proanthocyanidin can protect oxidative stress-injured retinal ganglion cells by inhibiting apoptotic process.

Persistent elevation of fibrosis biomarker cartilage oligomeric matrix protein following hepatitis C virus eradication

Serum levels of cartilage oligomeric matrix protein(COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has also been associated with hepatocellular carcinoma. We present a prospective analysis of this biomarker in38 patients with chronic hepatitis C who were subject to eradication therapy with direct acting antivirals. We confirm previous studies associating COMP elevation with liver cirrhosis. We also show how viral levels are correlated with COMP at baseline. In our prospective analysis, we report that successful eradication of hepatitis C results in improvement in liver stiffness and laboratory liver function tests at 1 year follow-up. In contrast, median COMP-levels remain unchanged during the study period. We conclude that the biomarker potential of COMP in the prospective evaluation of liver diseases, remains to be elucidated.

Synthesis and Luminescent Property of Polycarbazole/Polyhedral Oligomeric Silsesquioxane Nanocomposites

Polyvinylcarbazole（PVK） composites containing organic-inorganic hybrid polyhedral oligomeric silse-squioxane（POSS） PVK-POSS were prepared by free radical polymerization. POSS monomers reacted with vinylcarbazole and were completely dispersed at molecular level in PVK matrix and PVK-POSS nanocomposites display higher glass transition temperature（Tg） in comparison with neat PVK. Optical properties of PVK/POSS nanocomposites were investigated by UV-spectrum and PL-spectrum and the results show that the PVK-POSS nanoparticles have a good interface effect and improve color purity effectively. The maximum absorption wavelength bathochromically shifts gradually with the increasing of the content of POSS. The luminescent intensity becomes higher and higher with the increase of POSS content, and reaches its maximum luminescent intensity when the POSS content is 3% （mass fraction）, while some POSS-rich nanoparticles are present in matrix when contents of POSS are beyond 5%.

Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4

AIM: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4.METHODS: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining,terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcriptionpolymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed.RESULTS: In this study, cytotoxic effect of OPC on SNUC4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL)increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with control.CONCLUSION: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.

Altered serum level of cartilage oligomeric matrix protein and its association with coronary calcification in patients with coronary heart disease

BackgroundCartilage oligomeric 矩阵蛋白质(COMP ) 主要在骨胳的系统和脉管的光滑的肌肉房间被发现。最近的研究证明它在血容器上有保护的功能并且能也禁止脉管的石灰化。我们在冠的心疾病( CHD )调查了浆液 COMP 病人，并且在浆液 COMP 和首先经历了多片跟随的冠的 angiography 的 233 个连续的胸疼痛病人全部的冠的 artery.MethodsA 的石灰化之间的关系在六个月以内计算了断层摄影术( MSCT )被招募并且根据缩小百分比的冠的 angiography 钠直径划分了成二个组：CHD 组(缩小 50% 的直径， n = 194 ) 并且控制组(直径变窄 <50% ， n = 39 ) 。Gensini 分数，句法分数和冠的动脉钙分数(CAC ) 是计算的。浆液 COMP 水平用 COMP 的 ELISA.ResultsThe 层次被决定比在控制组在 CHD 组是显著地更高的 155.7 (124.5-194.5 ) ng/mL 对 128.4 (113.0-159.9 ) ng/mL， P = 0.019。在 COMP， Gensini 分数，句法分数，冠的狭窄的严厉和有狭窄的冠的动脉的数字之间没有关联 >50% 。浆液 COMP 与年龄被相关(r = 0.294， P < 0.001 ) ， fasting 葡萄糖(r = 0.163， P = 0.015 ) ， HbA1c (r = 0.194， P = 0.015 ) 并且 CAC (r = 0.137， P = 0.037 ) 。逐步的线性回归分析显示出那 COMP 水平，年龄是在 CHD 病人的 CAC 的独立预言者(= 0.402， t = 2.612， P = 0.015；= 0.472， t = 3.077， P = 0.005 ) 。为预言 CHD 的 COMP 的性能在曲线(AUC ) 下面作为区域被显示出：0.632， 95% CI：0.549-0.715 和上面的 tertile CAC 是 AUC：0.602， 95% CI：在操作冠的动脉的特征(巨鸟) 曲线 analysis.ConclusionCalcification 的接收装置的 0.526-0.678 是浆液 COMP 的一个独立预言者。

Hydration effect on the electronic transport properties of oligomeric phenylene ethynylene molecular junctions

A first-principles computational method based on the hybrid density functional theory is developed to simulate the electronic transport properties of oligomeric phenylene ethynylene molecular junctions with H2O molecules accumulated in the vicinity as recently reported by Na et al. [Nanotechnology 18 424001 （2007）]. The numerical results show that the hydrogen bonds between the oxygen atoms of the oligomeric phenylene ethynylene molecule and H2O molecules result in the localisation of the molecular orbitals and lead to the lower transition peaks. The H2O molecular chains accumulated in the vicinity of the molecular junction can not only change the electronic structure of the molecular junctions, but also open additional electronic transport pathways. The obvious influence of H2O molecules on the electronic structure of the molecular junction and its electronic transport properties is thus demonstrated.

Study of Flame Retardation of Polycarbonate Modified by Polyhedral Oligomeric Silsesquioxane and Caged Bicyclic Phosphate

The flame-retarded polycarbonate（PC） has been made with octaphenyl polyhedral silsesquioxane（OPS） and/or caged bicyclic phosphate（Trimer）.Thermal gravimetric analysis（TGA）,Fourier-transform infrared（FTIR）,TGA-FTIR,limiting oxygen index（LOI）,and mechanical tests have been employed to characterize the modified PC.The additives of OPS and Trimer in PC have been proved to be effective flame-retardants because of the synergistic interaction between the elements of P and Si.The role of OPS and Trimer in PC degradation are different：OPS participates in the charring of PC,while Trimer makes PC degrade in advance.In addition,OPS and Trimer induced obvious differences in the mechanical properties of PC.

Synthesis of Nonionic Oligomeric Manganese(Ⅱ) Complexes and Investigation of Their Toxicity andΤ_1-Relaxation Enhancement

Six nonionic oligomeric manganese （ Ⅱ ） complexes with oligomeric phosphate-polyglycol-EDTA ester ligands were synthesized and characterized. The longitudinal relaxivities of these complexes were measured. One of these complexes, which showed the highest relaxivity, was chosen to be used in the acute toxicity test and the T1-weighted imaging experiment. It has been found that compared to Gd-DTPA, this nonionic oligomeric Mn（ Ⅱ ） complex exhibits no acute toxicity, generates highly enhanced MRI signals and increases the intention time in the rat liver tissue.

Effects of Temperature and Energy on Stability of Oligomeric Enzyme Probed on Electrospray Ionization Mass Spectrometry

Escherichia coli 3-Deoxy-D-manno-octulosonate 8-phosphate（KDO8P） synthase catalyzed the condensation reaction between D-arabinose 5-phosphate（A5P） and phosphoenolpyruvate（PEP） to form KDO8P and inorganic phosphate（Pi）. The noncovalent tetrameric association ofKDO8P synthase was observed and dissociated in gas phase by means of electrospray ionization mass spectrometry under the very ＂soft＂ conditions. The results indicate that PEP-bound enzyme generated abundant tetrameric species as well as monomeric species at the ＂soft＂ conditions, whereas, the unbound enzyme favored the formation of a dimeric species. The mass spectra of the mixture of the enzyme with one of substrates, PEP, and A5P or one of products, KDO8P and Pi show that the complex of the unbound enzyme with PEP or Pi was prone to the formation of a monomeric species, whereas, that of the unbound enzyme with A5P or KDO8P was similar to the unbound enzyme. The intensity of the dimeric species increased with the increase of temperature at a collision voltage of 10 V. Taken together, the results presented here suggest that mass spectrometry will be a powerful tool to explore subtile conformational changes and/or subunit-subunit interactions of multiprotein assembly induced by ligand-binding and/or the changes of environmental conditions.

Functional foods effective for hepatitis C: Identification of oligomeric proanthocyanidin and its action mechanism

Hepatitis C virus(HCV)is a major cause of viral hepatitis and currently infects approximately 170 million people worldwide.An infection by HCV causes high rates of chronic hepatitis(>75%)and progresses to liver cirrhosis and hepatocellular carcinoma ultimately.HCV can be eliminated by a combination of pegylatedα-interferon and the broad-spectrum antiviral drug ribavirin;however,this treatment is still associated with poor efficacy and tolerability and is often accompanied by serious side-effects.While some novel direct-actingantivirals against HCV have been developed recently,high medical costs limit the access to the therapy in cost-sensitive countries.To search for new natural anti-HCV agents,we screened local agricultural products for their suppressive activities against HCV replication using the HCV replicon cell system in vitro.We found a potent inhibitor of HCV RNA expression in the extracts of blueberry leaves and then identified oligomeric proanthocyanidin as the active ingredient.Further investigations into the action mechanism of oligomeric proanthocyanidin suggested that it is an inhibitor of heterogeneous nuclear ribonucleoproteins(hn RNPs)such as hn RNP A2/B1.In this review,we presented an overview of functional foods and ingredients efficient for HCV infection,the chemical structural characteristics of oligomeric proanthocyanidin,and its action mechanism.

Mechanism Study of BF_(3)/n-Butanol-catalyzed 1-Decene Oligomerization Reaction

The active catalysts of the BF_(3)/n-C_(4)H_(9)OH-catalyzed 1-decene oligomerization reaction,as well as the distribution of the reaction products,was investigated by molecular simulation.The calculation results show that(BF_(3))_(2)·n-C_(4)H_(9)OH catalyzes the 1-decene oligomerization reaction with higher activity compared to BF_(3)·n-C_(4)H_(9)OH,which is the most catalytically active substance in the BF_(3)/n-C_(4)H_(9)OH catalyst system.The reaction energy barriers and heats of reaction of chain initiation,chain growth,and chain termination in BF_(3)/n-C_(4)H_(9)OH-catalyzed 1-decene oligomerization are calculated to reveal the product distribution.The calculation results show that the contents of the oligomerization reaction products in descending order are trimer,tetramer,pentamer,and dimer.The calculated results were consistent with the experimentally obtained product distribution.

荔枝落果中A型低聚原花青素抑制类甜蛋白促炎机制

以荔枝落果中的A型低聚原花青素为研究对象,探讨其对荔枝类甜蛋白(litchi thaumatin-like protein,LcTLP)促炎的抑制机理。采用乙醇提取、大孔吸附树脂纯化以及制备液相色谱仪等从荔枝落果中分离得到A型低聚原花青素(A-type oligomeric proanthocyanidins,A-OPC)纯化物,并对A-OPC结构鉴定,主要成分为原花青素A2、2个A型原花青素三聚体和1个A型四聚体,纯度达88.69%。利用LcTLP诱导构建RAW264.7细胞炎症模型,A-OPC的添加可抑制LcTLP诱导RAW264.7细胞的一氧化氮、细胞炎症因子白细胞介素-6和肿瘤坏死因子-α的分泌,在120μg/mL质量浓度下,抑制率分别可达58.38%、39.80%和86.31%(P<0.01)。蛋白免疫印迹分析发现,在120μg/mL质量浓度下A-OPC可降低诱导型一氧化氮合酶和环氧合酶2的表达量,并显著降低p65、核因子κB抑制蛋白、c-Jun氨基末端激酶、细胞外信号调节蛋白激酶和p38的磷酸化水平,说明A-OPC通过下调核转录因子-κB和丝裂原活化蛋白激酶通路中下游蛋白的磷酸化水平抑制LcTLP诱导的炎症反应。

褪黑素通过NF-κB/NLRP3信号抑制子宫内膜异位症的进展机制研究

目的:探讨褪黑素(MEL)对子宫内膜异位症(EMT)进展的抑制作用,以及其对核转录因子κB(NF-κB)/核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)信号的调控机制。方法:通过自体子宫内膜皮下种植法构建EMT大鼠。动物实验模型分假手术组(Sham组,大鼠在造模过程中仅进行子宫片段剪取)和实验处理4组,分别为:模型组(EMT组,大鼠进行自体子宫内膜皮下种植)、褪黑素组(EMT+MEL组,以50 mg/kg的MEL灌胃处理)、EMT+NF-κB信号通路激活剂佛波酯(PMA)组(EMT+PMA组,以5 mg/kg的PMA腹腔注射)、EMT+MEL+PMA组(以50 mg/kg的MEL灌胃处理和5 mg/kg的PMA腹腔注射)。检测各组大鼠子宫内膜异位的质量、血清和腹腔液中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量;免疫组化、免疫荧光检测各组样本中髓过氧化物酶(MPO)、血管内皮生长因子(VEGF)的表达;Western blot实验检测各组样本中NF-κB/NLRP3信号通路相关蛋白的表达。结果:与Sham组相比,实验处理4组大鼠中动情周期紊乱的比例、异位子宫内膜的质量、血清以及腹腔液中TNF-α和IL-6的含量、MPO和VEGF的表达及NF-κB/NLRP3信号通路相关蛋白的表达均明显升高,差异均有统计学意义(P<0.05)。与EMT组相比,EMT+MEL组和EMT+MEL+PAM组以上各项指标明显下降,而EMT+PAM组各项指标明显升高;与EMT+MEL组相比,EMT+MEL+PAM组、EMT+PAM组以上各项指标明显升高,以上差异均有统计学意义(P<0.05)。结论:MEL能明显抑制EMT中的炎症反应,抑制EMT的进展,这可能通过抑制NF-κB/NLRP3信号的激活实现的。

基于NOD2介导的AMPK/mTOR信号通路探讨宫颈癌细胞恶性行为的机制

目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC)以及NOD2过表达(NOD2)质粒和载体(Vec)转染CC细胞。通过CCK-8测定、集落形成和Transwell细胞侵袭测定来确定NOD2对CC细胞生长的影响。通过高通量RNA测序(RNA-Seq)进行转录组分析。Western blot试验检测细胞系中NOD2、AMPK/mTOR信号通路和自噬蛋白的表达。24只雌性BALB/c裸鼠随机分为4组,每组6只:载体组(Vec组)、NOD2过表达组(NOD2组)、shNC组和shNOD2组。构建小鼠远处转移模型,监测肺转移的荧光强度,计数肺转移结节的数量。结果 在线数据库分析显示,NOD2在CC组织中表达明显高于正常组织,并且不同分期的CC中NOD2的mRNA表达差异有统计学意义(P<0.05)。此外,NOD2的高表达与较差的总生存期和无病生存期相关(P<0.05)。NOD2过表达对CC细胞增殖、集落形成、迁移和侵袭具有促进作用,而NOD2敲低则相反。与体外结果一致,在转移的小鼠尾静脉注射模型中,NOD2组CC细胞的肺定殖、肺转移灶较Vec组增加(P<0.05),而shNOD2组CC细胞的肺定殖、肺转移灶较shNC组减少(P<0.05)。RNA-Seq结果显示NOD2表达与AMPK信号激活、mTOR信号抑制、自噬调节途径激活和自噬体形成显著相关。与shNC组相比,shNOD2组磷酸化AMPK、LC3蛋白表达水平减少(P<0.05),磷酸化mTOR、p62蛋白表达水平增加(P<0.05);与Vec组相比,NOD2组LC3、AMPK蛋白表达水平增加(P<0.05),磷酸化mTOR、p62蛋白表达水平减少(P<0.05)。与shNC组相比,shNOD2组GFP-mRFP-LC3的点积累减少(P<0.05);与Vec组相比,GFP-mRFP-LC3的点积累增加(P<0.05)。结论 NOD2可能通过AMPK/mTOR信号促进CC增殖、迁移和侵袭,其作用机制部分涉及自噬激活。

NLRP3炎症小体在大鼠单侧输尿管梗阻引起肾间质纤维化中的作用及其机制

目的:探讨核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎性小体在大鼠单侧输尿管梗阻(UUO)模型肾间质纤维化中的作用,并阐明其可能的作用机制。方法:健康雄性Wistar大鼠30只随机分为假手术组(n=6)和UUO组(n=24),假手术组大鼠仅分离输尿管不结扎,UUO组分别于术后3、7和14 d处死大鼠,并按照处理时间分为UUO 3 d组(n=8)、UUO 7 d组(n=8)和UUO 14 d组(n=8)。HE染色和Masson染色观察各组大鼠肾组织病理形态表现,试剂盒检测各组大鼠肾组织中丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性和羟脯氨酸(HYP)水平,免疫组织化学法检测各组大鼠肾组织中α-平滑肌肌动蛋白(α-SMA)和转化生长因子β1(TGF-β1)蛋白表达水平,Western blotting法检测各组大鼠肾组织中NLRP3蛋白表达水平。结果:HE染色,UUO组大鼠出现明显肾小管扩张,肾间质水肿和增宽,可见较多炎症细胞浸润,部分肾小管腔内可见脱落的上皮细胞。与假手术组比较,UUO 3 d组、UUO 7 d组和UUO 14 d组大鼠HE染色肾间质纤维化评分均明显升高(P<0.05);与UUO 3 d组和UUO 7 d组比较,UUO 14 d组大鼠HE染色肾间质纤维化评分明显升高(P<0.05)。Masson染色,UUO组大鼠肾间质炎症细胞浸润明显,可见明显纤维组织增生;随UUO作用时间增加,大鼠部分肾小管消失,肾间质明显增宽,胶原沉积逐渐增多,皮髓交界处胶原沉积程度更加明显。与假手术组比较,UUO 3 d组、UUO 7 d组和UUO 14 d组大鼠Masson染色肾间质纤维化评分均明显升高(P<0.05);与UUO 3 d组和UUO 7 d组比较,UUO 14 d组大鼠Masson染色肾间质纤维化评分明显升高(P<0.05)。与假手术组比较,UUO 3 d组、UUO 7 d组和UUO 14 d组大鼠梗阻侧肾组织中MDA水平均明显升高(P<0.05),SOD活性明显降低(P<0.05)。与假手术组比较,UUO 3 d组、UUO 7 d组和UUO 14 d组大鼠梗阻侧肾组织中HYP水平均明显升高(P<0.05);与UUO 3 d组比较,UUO 14 d组大鼠梗阻侧肾组织中HYP水平明显升高(P<0.05)。免疫组织化学法,与假手术组比较,UUO 3 d组、UUO 7 d组和UUO 14 d组大鼠肾组织中α-SMA蛋白表达水平明显升高(P<0.05);与UUO 3 d组和UUO 7 d组比较,UUO 14 d组大鼠肾组织中α-SMA蛋白表达水平均明显升高(P<0.05);与假手术组比较,UUO 3 d组、UUO 7 d组和UUO 14 d组大鼠肾小管上皮细胞和肾小管间质组织中TGF-β1蛋白表达水平明显升高(P<0.05);与UUO 3 d组比较,UUO 14 d组大鼠肾小管上皮细胞和肾小管间质组织中TGF-β1蛋白表达水平明显升高(P<0.05)。Western blotting法,与假手术组比较,UUO 7 d组和UUO 14 d组大鼠肾组织中NLRP3蛋白表达水平均明显升高(P<0.05)。结论:NLRP3炎症小体在UUO大鼠肾纤维化过程中发挥重要作用,其作用机制与氧化应激增加和TGF-β1蛋白表达水平升高有关。