Objective:To explore the expressions of c-fos and c-myc in skin lesion of cutaneous squamous cell carcinoma(CSCC).Methods:Using retrospective analysis.73 cases of CSCC were selected from Department of Dermatology,the ...Objective:To explore the expressions of c-fos and c-myc in skin lesion of cutaneous squamous cell carcinoma(CSCC).Methods:Using retrospective analysis.73 cases of CSCC were selected from Department of Dermatology,the Second Affiliated Hospital of Xi'an Jiaotong University.which were removed between January 2000 and January 2012.It was considered as experimental group.Meanwhile.11 cases of normal skin specimens of non tumor patients were selected as control group.The expression level of c-fos and c-myc was compared in the two groups.Results:The expressions of c-fos[72.60%(53/73)]and c-myc[83.56%(61/73)]in experimental group were statistically significant(P≤0.05)compared with control group(0%).Expression of c-myc protein was negatively related to differentiation of CSCC.The difference was statistically significant(X^2=7.26.P=0.001<0.05).While expression of c-fos protein was positively related to differentiation of CSCC.which was statistically significant(X^2=7.47,P=0.0012<0.025).Conclusions:The expression level of c-fos and c-myc can be used as an importan indicator of CSCC differentiation,and it has closely connection with the differentiated degree,which can guide clinical prognosis.展开更多
Objective: To study the distribution of c fos oncogene expression within central nervous system (CNS) of the rat during halothane anesthesia. Methods: c-fos oncogene immunohistochemical technique (ABC method) was empl...Objective: To study the distribution of c fos oncogene expression within central nervous system (CNS) of the rat during halothane anesthesia. Methods: c-fos oncogene immunohistochemical technique (ABC method) was employed. Results: When halothane concentration was 0.75%,1.5%or2.0%, most of the Fos-like immunore- active neurons (FLNs) appeared in telencephalon, diencephalon and brain stem, including cerebral cortex, amygaloid nucleus, accumbens nucleus, lateral keptal nucleus. bed nucleus of the stria terminalis, field CA1 of Ammon’s horn, islands of Calleja, paraventricular thalamic nucleus, central medial thalamic nucleus, reuniens thalamic nucleus, rhomboid thalamic nucleus. ventral posterolateral thalamic nucleus, paraventricular hypothalamic nucleus(ventral part). periventricular hypothalamic nucleus, median preoptic nucleus, supraoptic nucleus, suprachiasmatic nucleus. medial and lateral habenular nucleus. midbrain periaqueductal gray and Edinger-Westphal nucleus.In the present stude. we have also found that the number of FLN registered stable increase along with the increaseof the concentration of halothane. Conclusion: It has been indicated that FLNs participate in the process ofhalothane anesthesia. which should necessitate and pave the way for a further study of the patterns of linkage andthe mechanism of interaction between functional nuclei.展开更多
Objective To understand the regulation and control of the position of the diencophalon followingmethoxyflurane. halothane, enflurane and isoflurane anesthesia. Methods: By using Fos--oncoprotein antibody immunohistoch...Objective To understand the regulation and control of the position of the diencophalon followingmethoxyflurane. halothane, enflurane and isoflurane anesthesia. Methods: By using Fos--oncoprotein antibody immunohistochemical technique(ABC method), the distribution of c--fos oncogene expression in the diencophalon was observed under optical microscope (Olympus ). Results: Fifteen Fos--like immunoreactive neurons appeared in the diencophalon. They were Paraventricular thalanlic nucleus. central medial thalarnic nucleus, reuniens thalamic nucleus, rhomboid thalamic nucleus, dorsal endopiriform nucleus. dorsal lateralgeniculate nucleus. lateral posterior thalamic nucleus, laterdorsal thalamic nucleus, periventeriicular hypothealamic nucleus, median preoptic nucleus, supraoptic nucleus, arcute hyothalamic nucleus, suprachiasmatic nucleus, ventral posterolateral thalamic nucleus and habenular nucleus. Conclusion: The inhalationanesthetics in diencophalon do not spread all over the place but have clear acting positions.展开更多
基金Supported by Natural Science Foundation of Shaanxi Province(Grant No.2018722)
文摘Objective:To explore the expressions of c-fos and c-myc in skin lesion of cutaneous squamous cell carcinoma(CSCC).Methods:Using retrospective analysis.73 cases of CSCC were selected from Department of Dermatology,the Second Affiliated Hospital of Xi'an Jiaotong University.which were removed between January 2000 and January 2012.It was considered as experimental group.Meanwhile.11 cases of normal skin specimens of non tumor patients were selected as control group.The expression level of c-fos and c-myc was compared in the two groups.Results:The expressions of c-fos[72.60%(53/73)]and c-myc[83.56%(61/73)]in experimental group were statistically significant(P≤0.05)compared with control group(0%).Expression of c-myc protein was negatively related to differentiation of CSCC.The difference was statistically significant(X^2=7.26.P=0.001<0.05).While expression of c-fos protein was positively related to differentiation of CSCC.which was statistically significant(X^2=7.47,P=0.0012<0.025).Conclusions:The expression level of c-fos and c-myc can be used as an importan indicator of CSCC differentiation,and it has closely connection with the differentiated degree,which can guide clinical prognosis.
文摘Objective: To study the distribution of c fos oncogene expression within central nervous system (CNS) of the rat during halothane anesthesia. Methods: c-fos oncogene immunohistochemical technique (ABC method) was employed. Results: When halothane concentration was 0.75%,1.5%or2.0%, most of the Fos-like immunore- active neurons (FLNs) appeared in telencephalon, diencephalon and brain stem, including cerebral cortex, amygaloid nucleus, accumbens nucleus, lateral keptal nucleus. bed nucleus of the stria terminalis, field CA1 of Ammon’s horn, islands of Calleja, paraventricular thalamic nucleus, central medial thalamic nucleus, reuniens thalamic nucleus, rhomboid thalamic nucleus. ventral posterolateral thalamic nucleus, paraventricular hypothalamic nucleus(ventral part). periventricular hypothalamic nucleus, median preoptic nucleus, supraoptic nucleus, suprachiasmatic nucleus. medial and lateral habenular nucleus. midbrain periaqueductal gray and Edinger-Westphal nucleus.In the present stude. we have also found that the number of FLN registered stable increase along with the increaseof the concentration of halothane. Conclusion: It has been indicated that FLNs participate in the process ofhalothane anesthesia. which should necessitate and pave the way for a further study of the patterns of linkage andthe mechanism of interaction between functional nuclei.
文摘Objective To understand the regulation and control of the position of the diencophalon followingmethoxyflurane. halothane, enflurane and isoflurane anesthesia. Methods: By using Fos--oncoprotein antibody immunohistochemical technique(ABC method), the distribution of c--fos oncogene expression in the diencophalon was observed under optical microscope (Olympus ). Results: Fifteen Fos--like immunoreactive neurons appeared in the diencophalon. They were Paraventricular thalanlic nucleus. central medial thalarnic nucleus, reuniens thalamic nucleus, rhomboid thalamic nucleus, dorsal endopiriform nucleus. dorsal lateralgeniculate nucleus. lateral posterior thalamic nucleus, laterdorsal thalamic nucleus, periventeriicular hypothealamic nucleus, median preoptic nucleus, supraoptic nucleus, arcute hyothalamic nucleus, suprachiasmatic nucleus, ventral posterolateral thalamic nucleus and habenular nucleus. Conclusion: The inhalationanesthetics in diencophalon do not spread all over the place but have clear acting positions.
