Objective Chinese allergic subjects have high levels of sensitization to house dust mite （HDM） and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samp...Objective Chinese allergic subjects have high levels of sensitization to house dust mite （HDM） and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f I from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA.Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from 〈0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples.Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens.展开更多
Air pollutants can potentially lead to nitration of allergic proteins,thus promoting sensitization of these allergens.However,little is currently known about the nitration status of house dust mite(HDM)allergens.We id...Air pollutants can potentially lead to nitration of allergic proteins,thus promoting sensitization of these allergens.However,little is currently known about the nitration status of house dust mite(HDM)allergens.We identified the occurrence of nitrated products of two major HDM allergens Der f 1 and Der p 1 in dust samples collected from college dormitories in eastern China and assessed their associated health risk.The results showed that both non-nitrated and nitrated forms of the two allergens were detected in the dust in the range of non-detected(ND)-10.6,1.44-15.4,ND-22.4,ND-7.28μg/g for non-nitrated Der f 1,nitrated Der f 1,non-nitrated Der p 1 and nitrated Der p 1,respectively.The median rates of nitration were determined as 74.0%for Der f 1 and 20.4% for Der p 1 at consideration of one nitration site.Further analysis reveals that the levels of HDM allergens and their nitrated products were found to be generally higher during winter,in dormitories of lower altitude and with female occupants.Furthermore,the calculated risk indexes were at considerably high levels.Our findings suggest that nitrated HDM allergens have already accumulated in the environment at such significant levels and their associated health risk calls for our immediate attention.展开更多
Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of I...Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test （SPT） obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach （Periplaneta Americana, Per a） and 18.7% SPT positive to the German cockroach （Blattella germanica, Bla g）. The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite （Dermatophagoides pteronyssinus, Der p）. An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.展开更多
过敏原的硝基化会引起其致敏潜能的增强,进而带来更大的致敏性健康风险.过敏原蛋白质通常含有多个酪氨酸硝基化位点,分析过敏原硝基化的位点选择性是探究硝基化对过敏原致敏性影响的重要基础.本文以尘螨过敏原为研究对象,建立了基于超...过敏原的硝基化会引起其致敏潜能的增强,进而带来更大的致敏性健康风险.过敏原蛋白质通常含有多个酪氨酸硝基化位点,分析过敏原硝基化的位点选择性是探究硝基化对过敏原致敏性影响的重要基础.本文以尘螨过敏原为研究对象,建立了基于超高效液相色谱-串联质谱(UPLC-MS/MS)同时定量分析3种尘螨过敏原(Der f 1、Der p 1和Der p 2)的13个酪氨酸位点硝基化程度的方法,并应用于分析3种尘螨过敏原在过氧亚硝酸盐硝基化作用下的位点选择性.结果表明,3种尘螨过敏原均发生了位点特异性的硝基化,Y195、Y37和Y92分别为Der f 1、Der p 1和Der p 2中反应活性最高的硝基化位点.尘螨过敏原位点选择性的硝基化表明,在评价硝基化尘螨过敏原的致敏性变化时应当考虑其位点特异性的硝基化状况.展开更多
目的:获得尘螨变应原第6组分Der f 6原核表达产物并检测其与尘螨过敏性哮喘患儿血清抗体IgE结合率。方法:酶切质粒p ET28a(+)-Der f 6获得目的基因Der f 6,将其与p ET32a(+)载体连接成质粒p ET32a(+)-Der f 6,转化BL21细菌后,用异丙基...目的:获得尘螨变应原第6组分Der f 6原核表达产物并检测其与尘螨过敏性哮喘患儿血清抗体IgE结合率。方法:酶切质粒p ET28a(+)-Der f 6获得目的基因Der f 6,将其与p ET32a(+)载体连接成质粒p ET32a(+)-Der f 6,转化BL21细菌后,用异丙基硫代半乳糖苷(IPTG)诱导表达,用Ni+离子亲和层析柱纯化表达产物,用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、免疫印迹实验(Western blot)和蛋白质串联质谱(MALDI-TOF/TOF)鉴定纯化产物。以纯化获得的产物为包被抗原建立间接ELISA法检测尘螨过敏性哮喘患儿血清抗体反应情况。结果:成功构建了原核表达质粒p ET32a(+)-Der f 6,将该质粒转化E.coli BL21诱导表达,亲和层析纯化后,SDS-PAGE显示获得目的蛋白,Western blot验证其能够与载体的组氨酸标签结合,质谱鉴定其Der f 6结构一致。以此产物为包被抗原建立间接ELISA检测尘螨过敏性哮喘患儿血清,阳性率为41.3%(19/46)。结论:成功构建了原核表达质粒p ET32a(+)-Der f 6,亲和纯化获得的目的蛋白具有良好的反应原性。展开更多
文摘Objective Chinese allergic subjects have high levels of sensitization to house dust mite （HDM） and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f I from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA.Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from 〈0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples.Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens.
基金financially supported by the National Natural Science Foundation of China (No. 41977341)Natural Science Foundation of Zhejiang Province, China (No. LGF21B070007)Research Center for Air Pollution and Health, Zhejiang University (No. I21405-11)
文摘Air pollutants can potentially lead to nitration of allergic proteins,thus promoting sensitization of these allergens.However,little is currently known about the nitration status of house dust mite(HDM)allergens.We identified the occurrence of nitrated products of two major HDM allergens Der f 1 and Der p 1 in dust samples collected from college dormitories in eastern China and assessed their associated health risk.The results showed that both non-nitrated and nitrated forms of the two allergens were detected in the dust in the range of non-detected(ND)-10.6,1.44-15.4,ND-22.4,ND-7.28μg/g for non-nitrated Der f 1,nitrated Der f 1,non-nitrated Der p 1 and nitrated Der p 1,respectively.The median rates of nitration were determined as 74.0%for Der f 1 and 20.4% for Der p 1 at consideration of one nitration site.Further analysis reveals that the levels of HDM allergens and their nitrated products were found to be generally higher during winter,in dormitories of lower altitude and with female occupants.Furthermore,the calculated risk indexes were at considerably high levels.Our findings suggest that nitrated HDM allergens have already accumulated in the environment at such significant levels and their associated health risk calls for our immediate attention.
文摘Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test （SPT） obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach （Periplaneta Americana, Per a） and 18.7% SPT positive to the German cockroach （Blattella germanica, Bla g）. The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite （Dermatophagoides pteronyssinus, Der p）. An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.
文摘过敏原的硝基化会引起其致敏潜能的增强,进而带来更大的致敏性健康风险.过敏原蛋白质通常含有多个酪氨酸硝基化位点,分析过敏原硝基化的位点选择性是探究硝基化对过敏原致敏性影响的重要基础.本文以尘螨过敏原为研究对象,建立了基于超高效液相色谱-串联质谱(UPLC-MS/MS)同时定量分析3种尘螨过敏原(Der f 1、Der p 1和Der p 2)的13个酪氨酸位点硝基化程度的方法,并应用于分析3种尘螨过敏原在过氧亚硝酸盐硝基化作用下的位点选择性.结果表明,3种尘螨过敏原均发生了位点特异性的硝基化,Y195、Y37和Y92分别为Der f 1、Der p 1和Der p 2中反应活性最高的硝基化位点.尘螨过敏原位点选择性的硝基化表明,在评价硝基化尘螨过敏原的致敏性变化时应当考虑其位点特异性的硝基化状况.
文摘目的:获得尘螨变应原第6组分Der f 6原核表达产物并检测其与尘螨过敏性哮喘患儿血清抗体IgE结合率。方法:酶切质粒p ET28a(+)-Der f 6获得目的基因Der f 6,将其与p ET32a(+)载体连接成质粒p ET32a(+)-Der f 6,转化BL21细菌后,用异丙基硫代半乳糖苷(IPTG)诱导表达,用Ni+离子亲和层析柱纯化表达产物,用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、免疫印迹实验(Western blot)和蛋白质串联质谱(MALDI-TOF/TOF)鉴定纯化产物。以纯化获得的产物为包被抗原建立间接ELISA法检测尘螨过敏性哮喘患儿血清抗体反应情况。结果:成功构建了原核表达质粒p ET32a(+)-Der f 6,将该质粒转化E.coli BL21诱导表达,亲和层析纯化后,SDS-PAGE显示获得目的蛋白,Western blot验证其能够与载体的组氨酸标签结合,质谱鉴定其Der f 6结构一致。以此产物为包被抗原建立间接ELISA检测尘螨过敏性哮喘患儿血清,阳性率为41.3%(19/46)。结论:成功构建了原核表达质粒p ET32a(+)-Der f 6,亲和纯化获得的目的蛋白具有良好的反应原性。
