Introduction The tuber of Ophiopogon japonicus （ L. f） Ker- Gawl, which is a Chinese traditional medicine named ＂maidong＂ have been recorded to have various medical functions for curing cardiovascular diseases an...Introduction The tuber of Ophiopogon japonicus （ L. f） Ker- Gawl, which is a Chinese traditional medicine named ＂maidong＂ have been recorded to have various medical functions for curing cardiovascular diseases and bacterial infections, especially heart diseases. Phytochemical studies on this plant were reported previously. In search of new and bioactive components from Chinese traditional medicines, the tubers of Ophiopogon japonicus have been investigated in this study. The present paper article covers the isolation and structure elucidation of a new furospirostanol saponin, namely, Ophiofurospiside B （compound 1 ） and two known steroidal saponins （ compounds 2 and 3）.展开更多
Two new steroidal glucosides, 26-O-β-D-glucopyranosyl （25S）-furost-5-ene-1β,3β,22α,26-tetraol l-O-β-D-xylopyranosyl- （1 → 3）-[α-h-rhamnopyranosyl-（1 → 2）]-[β-D-fueopyranoside and （25R） spirost-5-ene-3...Two new steroidal glucosides, 26-O-β-D-glucopyranosyl （25S）-furost-5-ene-1β,3β,22α,26-tetraol l-O-β-D-xylopyranosyl- （1 → 3）-[α-h-rhamnopyranosyl-（1 → 2）]-[β-D-fueopyranoside and （25R） spirost-5-ene-3β,14α-diol-3-β-O-β-L-rhanmopyranosyl- （1 → 2）-[β-D-xylopyranosyl（1 → 4）]-[-β-D-glucopyranoside, were isolated from the Ophiopogon japonicus （L.f.） Ker-Gaw. Their structures were elucidated by spectroscopic methods.展开更多
Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid littl...Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus(L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off(MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry(UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone(PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate(%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.展开更多
基金Supported by Program for Changjiang ScholarsInnovative Research Team in University(No.IRT0413).
文摘Introduction The tuber of Ophiopogon japonicus （ L. f） Ker- Gawl, which is a Chinese traditional medicine named ＂maidong＂ have been recorded to have various medical functions for curing cardiovascular diseases and bacterial infections, especially heart diseases. Phytochemical studies on this plant were reported previously. In search of new and bioactive components from Chinese traditional medicines, the tubers of Ophiopogon japonicus have been investigated in this study. The present paper article covers the isolation and structure elucidation of a new furospirostanol saponin, namely, Ophiofurospiside B （compound 1 ） and two known steroidal saponins （ compounds 2 and 3）.
基金the National Nature Science Foundation of China (No.30772890)the Program of Progressing Beijing New Medicine Subject Group (XK100270569)the Key Project of Chinese Ministry of Education (No.108132).
文摘Two new steroidal glucosides, 26-O-β-D-glucopyranosyl （25S）-furost-5-ene-1β,3β,22α,26-tetraol l-O-β-D-xylopyranosyl- （1 → 3）-[α-h-rhamnopyranosyl-（1 → 2）]-[β-D-fueopyranoside and （25R） spirost-5-ene-3β,14α-diol-3-β-O-β-L-rhanmopyranosyl- （1 → 2）-[β-D-xylopyranosyl（1 → 4）]-[-β-D-glucopyranoside, were isolated from the Ophiopogon japonicus （L.f.） Ker-Gaw. Their structures were elucidated by spectroscopic methods.
基金supported by the National Natural Science Foundation of China(Nos.81473317,81274004)2011 Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Educationa Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus(L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off(MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry(UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone(PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate(%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.
