Multifunctional role of oral bacteria in the progression of nonalcoholic fatty liver disease

Non-alcoholic fatty liver disease(NAFLD)encompasses a spectrum of liver disorders of varying severity,ultimately leading to fibrosis.This spectrum primarily consists of NAFL and non-alcoholic steatohepatitis.The pathogenesis of NAFLD is closely associated with disturbances in the gut micr-obiota and impairment of the intestinal barrier.Non-gut commensal flora,particularly bacteria,play a pivotal role in the progression of NAFLD.Notably,Porphyromonas gingivalis,a principal bacterium involved in periodontitis,is known to facilitate lipid accumulation,augment immune responses,and induce insulin resistance,thereby exacerbating fibrosis in cases of periodontitis-associated NAFLD.The influence of oral microbiota on NAFLD via the“oral-gut-liver”axis is gaining recognition,offering a novel perspective for NAFLD management through microbial imbalance correction.This review endeavors to encapsulate the intricate roles of oral bacteria in NAFLD and explore underlying mechanisms,emphasizing microbial control strategies as a viable therapeutic avenue for NAFLD.

Assessing the host genetic background effects on type 2 diabetes and obesity development in response to mixed–oral bacteria and high-fat diet using the collaborative cross mouse model

Background: Host genetic background and sex, play central roles in defining the pathogenesis of type 2 diabetes(T2 D), obesity and infectious diseases. Our previous studies demonstrated the utilization of genetically highly diverse inbred mouse lines, namely collaborative cross(CC), for dissecting host susceptibility for the development of T2 D and obesity, showing significant variations following high-fat(42% fat) diet(HFD). Here, we aimed to assessing the host genetic background and sex effects on T2 D and obesity development in response to oral-mixed bacterial infection and HFD using the CC lines.Materials and Methods: Study cohort consists of 97 mice from 2 CC lines(both sexes), maintained on either HFD or Standard diet(CHD) for 12 weeks. At week 5 a group of mice from each diet were infected with Porphyromonas gingivalis(Pg) and Fusobacterium nucleatum(Fn) bacteria(control groups without infection). Body weight(BW) and glucose tolerance ability were assessed at the end time point of the experiment.Results: The CC lines varied(P <.05) at their BW gain and glucose tolerance ability(with sex effect) in response to diets and/or infection, showing opposite responses despite sharing the same environmental conditions. The combination of diet and infection enhances BW accumulation for IL1912, while restraints it for IL72. As for glucose tolerance ability, only females(both lines) were deteriorated in response to infection.Conclusions: This study emphasizes the power of the CC mouse population for the characterization of host genetic makeup for defining the susceptibility of the individual to development of obesity and/or impaired glucose tolerance.

Oral Bacteria Are Responsible for the Etiology of Rheumatoid Arthritis

A significant association has been shown to exist between periodontitis and rheumatoid arthritis (RA) in observational studies. Some intervention studies have suggested that periodontal treatment can reduce serum inflammatory biomarkers such as C-reactive protein, or erythrocyte sedimentation rate. Serum antibodies to oral bacteria have been found at increased levels in RA patients. In addition, DNAs from these bacteria have been identified from samples of RA patients. Several antibiotics are effective on RA. Finally, oral bacteria are responsible for the etiology of RA.

In vitro Antibacterial Efficacy of <i>Bidens pilosa</i>, <i>Ageratum conyzoides</i>and <i>Ocimum suave</i>Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda

The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS patients’ oral bacteria. Healthy green leaves of the plants were collected in Ishaka Uganda, processed and portions separately extracted with hot distilled water and cold ethanol. The susceptibility, MIC and MBC of each extract were determined using standard protocols. The bacteria had significant (p Bidens pilosa, Ageratum conyzoides and Ocimum suave on oral lesions of HIV/AIDS patients made by traditional healers and local people in South-Western Uganda. We recommend a detailed study of structural identities and activities of the active antibacterial principle(s) in these plants for possible new drug entities and verification of the interactive effects of the principle(s) with ARVs and cotrimoxazole used daily by HIV/AIDS patients.

Brain abscess from oral microbiota approached by metagenomic next-generation sequencing: A case report and review of literature

BACKGROUND Brain abscess is a serious and potentially fatal disease caused primarily by microbial infection.Although progress has been made in the diagnosis and treatment of brain abscesses,the diagnostic timeliness of pathogens needs to be improved.CASE SUMMARY We report the case of a 54-year-old male with a brain abscess caused by oral bacteria.The patient recovered well after receiving a combination of metagenomic next-generation sequencing(mNGS)-assisted guided medication and surgery.CONCLUSION Therefore,mNGS may be widely applied to identify the pathogenic microor-ganisms of brain abscesses and guide precision medicine.

Metabolic fate of dietary sialic acid and its infuence on gut and oral bacteria

In recent years,the biological functions of human milk oligosaccharides and the potential toxic efects of red meat on human health have attracted considerable attention.Sialic acid is an important carbohydrate in milk and red meat,corresponding to sialylated oligosaccharides and N-glycolylneuraminic acid(NeuGc,one type of sialic acid).Herein,we reviewed the metabolic fate of dietary sialic acid in the body and their efects on gut and oral microbes.In summary,dietary NeuAc monomer is directly excreted through urine after being assimilated through the intestines and is not utilized by the human body;in contrast,dietary NeuGc from red meat is easily utilized by the human body and can be incorporated into the brain and other organs.Sialoglycans can be partially utilized by the human body,but they do not afect the cognitive development and growth of children.Dietary sialic acid may mainly regulate the growth and metabolism of gastrointestinal microbiota and human health and development through the gut–brain axis.

Effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria

Objective: To investigate the effect of the roselle calyx extract(RCE)(Hibiscus sabdariffa L.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria. Methods:RCE was prepared by soaking roselle calyx powder with ethyl alcohol for 24 h at room temperature. After centrifugation, the extract was lyophilized. Then, the extract was dissolved in phosphate-buffered saline, the p H was adjusted, and the extract was aseptically filtered. We used Streptococcus mutans, Streptococcus sanguinis, Lactobacillus casei, Actinomyces naeslundii, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in this study. The antibacterial activity of the RCE was determined by treating the cells of these bacteria with the extract for 10 or 20 min at room temperature. The minimum inhibitory concentration(MIC) and minimum bactericidal concentration was determined using the micro dilution method, and the effect of the RCE on the ability to form biofilm was determined using a polystyrene micro plate assay. In addition, we used the WST-1 assay to determine the cytotoxicity of the RCE on HGF, Ca9-22 and KB cells. Results: The RCE had antibacterial activity against oral bacteria used in this study. In particular, most significant antibacterial activity was observed against Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis. The MIC and minimum bactericidal concentration were 7.2 mg/m L–28.8 mg/m L and 14.4 to >57.6 mg/m L. The RCE had an inhibitory effect on biofilm formation at the MIC and sub-MIC levels. In addition, the RCE had low cytotoxic effects on HGF, Ca9-22 and KB cells. Conclusions: Thus, our results indicate that the RCE may be used for preventing oral diseases.

Synergistic effect between cryptotanshinone and antibiotics in oral pathogenic bacteria

Cryptotanshinone (CT), a major tanshinone of medicinal plant Salvia miltiorrhiza Bunge, demonstrated effective in vitro antibacterial activity against all oral bacteria tested in this experiment. The antibacterial activities of CT against oral bacteria were assessed using the checkerboard and time-kill methods to evaluate the synergistic effects of treatment with ampicillin or gentamicin. The CT was determined against oral pathogenic bacteria with MIC and MBC values ranging from 0.5 to 16 and 1 to 64 μg/mL;for am- picillin from 0.0313 to 16 and 0.125 to 32 μg/mL;for gentamicin from 2 to 256 and 4 to 512 μg/mL respectively. The range of MIC50 and MIC90 were 0.0625 - 8 μg/mL and 1 - 64 μg/mL, respectively. The combination effects of CT with antibiotics were synergistic (FIC index < 0.5) against tested oral bacteria except additive, Streptococcus sobrinus, S. criceti, and Actinobacillus actinomycetemcomitans (FIC index < 0.75 - 1.0). The MBCs were shown reducing ≥4 - 8-fold, indicating a synergistic effect as defined by a FBCI of ≤0.5. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 3 - 6 h of treatment with the 1/2 MIC of CT, regardless of whether it was administered alone or with ampicillin or gentamicin. The results suggest that CT could be employed as a natural antibacterial agent against cariogenic and periodontopathogenic bac- teria.

慢性萎缩性胃炎与消化道菌群的研究进展

慢性萎缩性胃炎(CAG)被认定为胃癌的癌前状态,该病在全球的发病率不断攀升,对其发病机制研究、生物学诊断技术及治疗药物的革新势在必行。相关消化道菌群微生态的研究极有可能推动这一革新。随着对幽门螺杆菌(Hp)研究的深入,人们发现单独干预Hp并不能完全延缓萎缩性胃炎进展,由此口腔菌群、胃内非Hp菌群及肠道菌群组成的“口-胃-肠”菌群微生态轴在CAG中的表观特征逐步得到重视。本文通过查阅中国知网、万方、维普、PubMed等数据库自2000—2023年的相关文献,筛出48篇目标文献,并将对口腔及舌苔菌群、胃内非Hp菌群和肠道菌群3个方面与CAG的关系进行回顾,同时探讨中医药基于调节菌群对CAG治疗的可行性,展望“胆汁酸-菌群轴”在未来CAG的菌群研究中将发挥的重要作用。

氯己定在口腔细菌中敏感性下降及其相关机制的研究进展

氯己定(chlorhexidine,CHX)被广泛应用于医疗领域的表面消毒,然而其应用缺乏临床限制。其长期广泛应用已导致多种重要的院内感染病原体临床分离菌株出现CHX敏感性下降,并可产生对一系列关键抗生素的交叉耐药。然而,CHX已在口腔领域作为金标准应用超过50年,其在口腔细菌中的耐药风险却未被重视。因此,本文旨在回顾CHX在口腔细菌中敏感性下降的研究进展并对其相关机制进行阐述,引发口腔领域对于CHX应用潜在风险的关注。

正畸患者口腔细菌感染情况及对血清、龈沟液PAK5、IL-6、IL-8水平的影响

目的分析正畸患者口腔细菌感染情况及对血清、龈沟液p2l活化激酶(PAK5)、白介素-6(IL-6)、白介素-8(IL-8)水平的影响。方法回顾性分析2020年12月至2022年12月间聊城市第四人民医院口腔科门诊收治的446例正畸治疗患者临床资料,分析治疗1周后患者口腔感染情况,并按是否感染分为感染组和非感染组,分离并培养感染组患者口腔感染病原菌,分析病原菌分布情况,并记录药敏试验结果。比较两组治疗前、治疗1周后龈沟液、血清IL-6、IL-8、PAK5水平,绘制ROC曲线评估龈沟液、血清IL-6、IL-8、PAK5预测口腔感染的效能。结果纳入患者中,感染45例,感染率为10.09%;45例正畸治疗口腔感染患者共培养分离出83株病原菌,有3例患者合并2种病原菌感染;革兰阳性菌38株,占比45.78%;革兰阴性菌45株,占比54.22%;病原菌分布中占比前三位是肺炎克雷伯菌、表皮葡萄球菌、牙龈卟啉单胞菌;革兰阳性菌中青霉素耐药率最高,革兰阴性菌中阿莫西林/克拉维酸耐药率最高。治疗前,两组龈沟液、血清IL-6、IL-8、PAK5水平比较差异均无统计学意义(P>0.05);治疗1周后,两组龈沟液、血清IL-6、IL-8、PAK5均高于治疗前,且感染组高于非感染组,差异有统计学意义(P<0.05)。ROC曲线显示:治疗后龈沟液、血清IL-6、IL-8、PAK5是预测正畸治疗口腔感染的有效指标(P<0.05)。结论正畸患者口腔感染病原菌中革兰阴性菌占比较高,监测正畸患者治疗1周后的龈沟液及血清IL-6、IL-8、PAK5水平有利于评估其口腔感染发生风险。

防根面龋牙科材料的研究进展

根面龋是老年人口腔常见病和多发病,发病率和未治疗率高,治疗难度大,可迅速进展引起牙髓根尖周病或残冠根,是老年人失牙的重要原因,已成为严重影响老年人生活质量的常见口腔慢性病之一。因此,根面龋早期干预和防治非常重要。传统防根面龋牙科材料如氟化物、氯己定等在临床中广泛应用,但是其具有牙齿着色、再矿化及杀菌效率低下等缺点。近年来,一系列新兴防根面龋牙科材料如无机纳米材料、胶原交联剂等逐步成为研究热点,新兴材料具有促进深部牙体组织再矿化、作用时间延长、黏附力增强等优点。未来防龋材料应根据根面龋的特点及应用人群进行设计,朝着简便化、高效化、低毒性方面发展。本文对目前防根面龋牙科材料的研究及应用进行综述,以期能够为防根面龋牙科材料的研究提供理论基础,对促进根面龋的有效预防,改善老年人的口腔健康状况和生活质量具有重要意义。

南阳市口腔医院2021-2022年口腔疾病患者病原菌感染及耐药性分析

目的:分析南阳市口腔医院2021-2022年口腔疾病患者的病原菌分布特征及其耐药性。方法:选取2021年1月至2022年12月南阳市口腔医院收治的口腔疾病患者179例,采集患者口腔分泌物,进行病原菌培养和药敏试验,分析病原菌分布特征和耐药性。结果:179例口腔疾病患者,共分离出58株病原菌;革兰阴性菌17株,占比29.31%,以铜绿假单胞菌(5.17%)、肺炎克雷伯菌(8.62%)为主;革兰阳性菌41株,占比70.69%,以表皮葡萄球菌(13.79%)、星群链球菌星群亚种(20.69%)为主。铜绿假单胞菌对阿米卡星、庆大霉素、妥布霉素耐药率较高,对环丙沙星、他唑巴坦、多黏菌素B及头孢他啶耐药性均为0.00%;肺炎克雷伯菌对阿米卡星、舒巴坦、环丙沙星、庆大霉素、妥布霉素及头孢他啶耐药率较高,对他唑巴坦、多黏菌素B均为0.00%。表皮葡萄球菌对阿奇霉素、红霉素、青霉素耐药率较高,星群链球菌星群亚种对达托霉素、美罗培南、利奈唑胺、青霉素、氯霉素及利福平无耐药性,表皮葡萄球菌对达托霉素、美罗培南、利奈唑胺、利福平无耐药性。结论:南阳市口腔医院2021–2022年口腔疾病患者病原菌感染种类较多,主要以革兰阳性为主,临床需参照病原菌检测结果,结合病原菌耐药状况,挑选合理的抗菌药物进行抗感染治疗。

抗龋齿口腔益生乳酸菌的筛选

本研究旨在筛选出抑制口腔致病菌能力强、安全性高、口腔定植能力强的乳酸菌。首先,采用牛津杯琼脂扩散法从实验室乳酸菌菌种资源库中筛选抑制变异链球菌和戈氏链球菌的菌株,对抑菌性较高的28株乳酸菌菌株进行了溶血性评价,结果显示7株菌株呈现α-溶血,21株菌株无溶血性。通过中和处理、过氧化氢酶以及蛋白酶敏感性研究,探究无溶血性且对变异链球菌和戈氏链球菌抑菌圈直径≥10 mm的16株乳酸菌的抑菌代谢产物,筛选出7株抑菌能力强并有可能产生非有机酸类抑菌代谢产物的乳酸菌。安全性评价结果显示:7株乳酸菌产酸性以及对牙齿腐蚀性均较低,除菌株JZ14-1对溶菌酶最高耐受质量浓度为1.6 mg/mL,其他6株乳酸菌对溶菌酶最高耐受质量浓度为1.2 mg/mL,均可耐受溶菌酶。菌株HB13-2敏感的抗生素数量最多。口腔定植能力评价显示:菌株HB13-2具有最高的表面疏水性为44.00%,表面酸电荷为20.16%,表面碱电荷为49.44%,胞外多糖质量浓度为0.283 mg/mL;菌株HB13-2具有较低的自身生物膜形成能力和良好的自聚能力,易定植于口腔。菌株HB13-2对变异链球菌生物膜最高抑制率为59.72%。除此以外,具有较强的与变异链球菌共聚能力,在24 h共聚能力达到67.40%。综上,菌株HB13-2抑菌活性好、安全性高、口腔定植能力强,经16S rDNA测序鉴定为植物乳植杆菌,并命名为植物乳植杆菌(Lactiplantibacillus plantarum)HB13-2。

Isolation and Antibiotic-Resistant Pattern of Opportunistic Infectious Microbes from the Infected Sites of Oral Cancer Patients Compared to That of Healthy People Oral Microbiota

Introduction: Oral cancer is the third most prominent type of cancer in Bangladesh. During or after oral cancer treatment, immune-compromised cancer patients may be susceptible to an infection by multi-drug-resistant opportunistic microbes. This study aims to identify the prevalent microorganisms from the infected site of oral cancer patients and observe their antibiotic susceptibility pattern. Materials and Methods: Clinical samples were collected from the infected sites of oral cancer patients and healthy people. The swabs collected were placed on nutrient agar slant, then incubated for 24 hours at 37ºC. Bacteria from the slant were inoculated in several selective media (Mannitol Salt Agar Media, KF Streptococcus Agar media, Cetrimide Agar, Eosin Methylene Blue Agar). Several biochemical tests identified opportunistic microorganisms. Finally, the antibiotic susceptibility test was performed by the Kirby Bauer disc diffusion method. Results: The study found that 65.4% of microbes isolated from the patients’ oral cavities were Gram-negative bacteria, and 34.6% were Gram-positive bacteria. Among the patient group isolates (n = 55), the most prevalent organism was Pseudomonas spp. (30;54.54%). Others were Klebsiella spp. (27;49.09%), Staphylococcus spp. (24;43.63%), E. coli (14;25.45%), Streptococcus spp. (14;25.45%), Proteus spp. (12;21.8%) and Enterococcus spp. (6;10.90%). Both Gram-positive and Gram-negative microbes from the patient group have shown high resistance to the commonly used antibiotics. In the control group (n = 50), the most prevalent organism was Staphylococcus spp. (15;30%). Other organisms were Streptococcus spp. (6;12%), Klebsiella spp. (11;22%), E. coli (3;6%), Streptococcus spp. (14;25.45%), Pseudomonas spp. (8;16%). The microbes of the control group showed less resistance to the antibiotics and rather showed sensitivity to them. Conclusion: The study revealed a high prevalence of multi-drug-resistant opportunistic microbes on immune-compromised oral cancer patients compared to microbes isolated from healthy people’s oral cavity.

驼乳源乳铁蛋白嵌合肽对口腔致龋菌抗菌作用的初步探究

该研究旨在评估驼乳源乳铁蛋白(lactoferrampin-lactoferricin,LFA-LFC)嵌合肽对变异链球菌、唾液链球菌和远缘链球菌的抗菌效果并初步探究其作用机制。首先预测并鉴定驼乳源LFA-LFC嵌合肽的分子特性和二级结构;通过测定嵌合肽最低抑菌(minimum inhibitory concentration,MIC)、杀菌浓度(minimum bactericidal concentration,MBC),绘制时间-致死曲线,评估嵌合肽的抗菌效果;分析嵌合肽对生物膜的预防、消除作用。采用电子显微镜观察嵌合肽对细菌的作用位点,测定菌体DNA迁移率,对嵌合肽的抗菌机制进行初步研究。结果表明,驼乳源LFA-LFC嵌合肽对变异链球菌、唾液链球菌和远缘链球菌的最低抑菌浓度分别为32、32、64μmol/L;最低杀菌浓度分别为128、128、256μmol/L;细菌在1×MIC嵌合肽浓度处理30 min后完全致死;当嵌合肽浓度为128μmol/L时预防生物膜生成约80%,512μmol/L时消除生物膜约50%;4×MIC嵌合肽浓度处理细菌后发现其细胞膜均出现裂解,DNA未发生迁移。该研究认为驼乳源LFA-LFC嵌合肽对3株菌表现出显著的抗菌效果,嵌合肽可通过破坏细菌细胞膜,进入细胞内部作用于DNA,抑制细菌生长繁殖。

牙龈卟啉单胞菌第Ⅸ型分泌系统结构与功能研究进展

牙龈卟啉单胞菌第Ⅸ型分泌系统(type Ⅸ secretion system,T9SS)是一种最近被发现的新型蛋白分泌系统,它广泛分布于拟杆菌中。T9SS结构复杂、功能强大,目前已经得到验证的T9SS组分20余种,并且仅在牙龈卟啉单胞菌中即能分泌30余种毒力因子,对牙龈卟啉单胞菌的致病性意义重大。T9SS是一个横跨细胞内膜、细胞周质、细胞外膜的大型蛋白复合体,通过各组分之间结构和功能上的联系,组成了一个包括动力提供、能量转导、内外膜易位、外膜修饰及调节系统的精密功能复合体,对货物蛋白进行识别、转运、剪切、修饰,并将细菌胞内货物蛋白转运至细胞表面。近年来,随着X线衍射及原位冷冻电镜技术的发展,对T9SS的探究也从单一组分的功能研究发展至多蛋白复合体原位结构研究,但是目前对蛋白结构解析仍存在分辨率不高、无法捕捉动态功能结构等问题,未来研究方向应该更集中于探索T9SS如何与货物蛋白相互作用并发挥功能。本文对牙龈卟啉单胞菌T9SS在X线衍射及冷冻电镜结构解析上的研究进展做一综述,以便深入理解T9SS的转运机制。

抗菌肽在龋病防治中的研究进展

抗菌肽是一种具有抗菌作用的肽类物质,可分为天然抗菌肽和人工合成抗菌肽两种。人工合成抗菌肽主要是根据天然抗菌肽结构,通过重组不同功能结构域、调整原有氨基酸序列,或者完全重新设计,而获得新的抗菌肽。抗菌肽可抑制多种致龋微生物的生长,抑制微生物生物膜的形成,降低微生物产酸耐酸的特性。天然抗菌肽基因可作为龋病遗传易感性标志物,在口腔龋病防治方面具有良好的应用前景,但天然抗菌肽的不稳定性、无法实现靶向缓释,限制了其在口腔龋病防治中的应用。人工合成抗菌肽可提高抗菌肽稳定性和对微生物的杀灭作用;可与口腔常用粘接剂聚合,减少龋病充填治疗后的微渗漏,防止继发龋的发生;可根据龋病动态进展中的pH值敏感性靶向缓释,促进早期龋的再矿化。但人工合成抗菌肽的安全性和生物相容性较天然抗菌肽差;抗菌肽与其他龋病防治药物,如氟化物等联用效果尚不确切。本文针对目前抗菌肽的种类、防龋作用机制和改良设计方法进行综述,为龋病防治提供新方法与新思路。

结肠靶向给药系统在粪菌移植中的应用

粪菌移植(FMT)对多种疾病有独特的疗效,其常见的治疗途径主要有口服、鼻肠管、结肠镜、灌肠等。其中口服服用方便,多次服用相对损伤小,但是口服粪菌如果在胃或小肠中崩解会影响FMT效果。结肠靶向给药系统(CTDDS)使药物更准确地作用于结肠,能提高治疗效果,减少毒副作用。CTDDS除了传统的pH敏感型、时滞型、酶触发型、压力依赖型等外,随着研究的深入还发展出了磁驱动型、配(受)体介导型、纳米药物型等。近年来一些研究将二者结合,使用CTDDS进行FMT,取得了一定的疗效,该文系统介绍了CTDDS在FMT中的应用。

不同浓度氯己定口腔护理液在重症监护室病人中的应用

目的:探讨不同浓度氯己定口腔护理液在重症监护室病人中的应用效果。方法:选取2019年6月—2019年12月在山西省汾阳医院重症监护室(ICU)住院行机械通气的84例病人为研究对象,随机分为生理盐水组、0.12%氯己定组、0.20%氯己定组、2.00%氯己定组,各21例,分别使用相应的口腔护理液干预,比较4组口腔情况、细菌定植及呼吸机相关性肺炎发生率。结果:4组护理前及护理后第1天、第2天、第3天、第4天口腔评估指标量表(OAG)评分比较差异有统计学意义(P<0.05);4组铜绿假单胞菌及鲍曼不动杆菌定植情况差异有统计学意义(P<0.05);4组呼吸机相关性肺炎发生率差异无统计学意义(P>0.05)。结论:不同浓度的氯己定溶液及生理盐水均有助于改善ICU病人的口腔环境,0.12%氯己定口腔护理液抑制口腔鲍曼不动杆菌作用显著。