The effects of phenanthrene（Phe） on hepatosomatic index（HSI） and hepatic enzymatic activities in hybrid tilapia（Oreochromis niloticus ♀× O.aureus ♂） were investigated via the static freshwater exposure at...The effects of phenanthrene（Phe） on hepatosomatic index（HSI） and hepatic enzymatic activities in hybrid tilapia（Oreochromis niloticus ♀× O.aureus ♂） were investigated via the static freshwater exposure at dosage of 50,100,and 400 μg/L for 4-14 d.Compared with the control group,HSI was significantly decreased（P 〈 0.05） at 400 μg/L at day 14.Increased enzymatic activities（P 〈 0.05） were observed for catalase（CAT）,glutathione peroxidase（GPx） and superoxide dismutase（SOD） at either 100 or 400 μg/L at day 8 and 14,as well as for CAT at 50 μg/L at day 14,except for GPx at 400 μg/L at day 8.Ethoxyresorufin O-deethylase（EROD） activity was significantly increased（P 〈 0.05） at all dosage at day 4 as well as at 50 μg/L at day 8,but significantly decreased at either 100 or 400 μg/L at day 14（P 〈 0.05）.Glutathione-S-transferase（GST） activity was not affected.The results suggest that CAT,GPx,SOD and EROD,as well as HSI in tilapia may be used as the biomarkers or indexes for evaluating or monitoring the pollution of polycyclic aromatic hydrocarbons（PAHs） such as Phe.展开更多
基金supported by the National Natural Science Foundation of China (No. 30570325,30671629)the Science and Technology Project of Guangdong Province (No.2005B20301013, 2006A36502004,2007B090400049)
文摘The effects of phenanthrene（Phe） on hepatosomatic index（HSI） and hepatic enzymatic activities in hybrid tilapia（Oreochromis niloticus ♀× O.aureus ♂） were investigated via the static freshwater exposure at dosage of 50,100,and 400 μg/L for 4-14 d.Compared with the control group,HSI was significantly decreased（P 〈 0.05） at 400 μg/L at day 14.Increased enzymatic activities（P 〈 0.05） were observed for catalase（CAT）,glutathione peroxidase（GPx） and superoxide dismutase（SOD） at either 100 or 400 μg/L at day 8 and 14,as well as for CAT at 50 μg/L at day 14,except for GPx at 400 μg/L at day 8.Ethoxyresorufin O-deethylase（EROD） activity was significantly increased（P 〈 0.05） at all dosage at day 4 as well as at 50 μg/L at day 8,but significantly decreased at either 100 or 400 μg/L at day 14（P 〈 0.05）.Glutathione-S-transferase（GST） activity was not affected.The results suggest that CAT,GPx,SOD and EROD,as well as HSI in tilapia may be used as the biomarkers or indexes for evaluating or monitoring the pollution of polycyclic aromatic hydrocarbons（PAHs） such as Phe.
