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转OsCDPK7基因水稻的培育与耐盐性分析 被引量:13
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作者 王镭 才华 +3 位作者 柏锡 李丽文 李勇 朱延明 《遗传》 CAS CSCD 北大核心 2008年第8期1051-1055,共5页
以4℃处理的水稻品种辽盐241植株叶片总RNA为模板,用基因特异引物通过RT-PCR扩增出1700bp的OsCDPK7基因。该基因序列比已报道的基因序列(GenBank登录号:AB042550)缺失了26个氨基酸,而丝氨酸/苏氨酸蛋白激酶活性中心和钙结合结构域完整,... 以4℃处理的水稻品种辽盐241植株叶片总RNA为模板,用基因特异引物通过RT-PCR扩增出1700bp的OsCDPK7基因。该基因序列比已报道的基因序列(GenBank登录号:AB042550)缺失了26个氨基酸,而丝氨酸/苏氨酸蛋白激酶活性中心和钙结合结构域完整,具备钙依赖的蛋白激酶活性。构建了由组成型启动子E12调控的OsCDPK7基因植物表达载体,利用农杆菌介导法转化水稻,经Km筛选及Southern杂交验证,获得10株转基因植株。耐盐性分析表明:OsCDPK7基因的组成型表达提高了T2代转基因植株的耐盐性,部分转基因水稻在0.2mol/LNaCl培养基中能够萌发;幼苗期水稻经0.4mol/LNaCl浇灌10d,去除胁迫后能恢复正常生长;而对照在以上情况下均不能萌发和恢复。结果表明,利用植物信号转导过程中的调控因子能够提高转基因作物的耐盐性。然而,在不同耐性的转基因植株中,OsCDPK7基因的表达有一定的差异。 展开更多
关键词 oscdpk7基因 转基因水稻 耐盐性
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转耐盐基因OsCDPK7、OsMAPK4水稻农艺性状调查与分析 被引量:1
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作者 赵阳 才华 +2 位作者 柏锡 纪巍 朱延明 《作物杂志》 CAS CSCD 北大核心 2012年第5期22-25,共4页
在前期已获得耐盐转OsCDPK7、OsMAPK4基因水稻的基础上,对转基因株系的产量性状、生物学性状、品质性状和生育期等农艺性状进行调查与分析。结果表明,在田间栽培条件下,转基因水稻植株个体生长发育正常,转OsCDPK7基因水稻株系主要农艺... 在前期已获得耐盐转OsCDPK7、OsMAPK4基因水稻的基础上,对转基因株系的产量性状、生物学性状、品质性状和生育期等农艺性状进行调查与分析。结果表明,在田间栽培条件下,转基因水稻植株个体生长发育正常,转OsCDPK7基因水稻株系主要农艺性状与对照无差异;而转OsMAPK4基因水稻株系与其受体之间单位面积产量、株高、有效穗数和粗蛋白含量等均存在显著差异。这种差异与OsMAPK4基因功能存在必然关系。 展开更多
关键词 水稻 耐盐转基因株系 oscdpk7 OsMAPK4 农艺性状
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Identification of a Novel OsCYP2 Allele that Was Involved in Rice Response to Low Temperature Stress 被引量:2
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作者 Hongxiu Gao Lin Zhu +7 位作者 Tianqi Liu Xueyu Leng Zhenxing Zhu Wei Xie Haitao Lv Zhengxun Jin Ping Wu Zhongchen Zhang 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第6期1743-1763,共21页
Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is... Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is involved in rice response to low temperature is still unclear.We identified a new OsCYP2 allelic mutant,lrl3,with fewer lateral roots,and the differences in shoot height,primary root length and adventitious root length increased with the growth process compared to the wild-type plant.Auxin signaling pathway was also affected and became insensitive to gravity.The transgenic rice plants with over-expression of OsCYP2 were more tolerant to low temperature than the wild-type plants,suggesting that OsCYP2 was involved in the low temperature response in rice.In addition,OsCYP2 negatively regulated the expression of OsTPS38,a terpene synthase gene,and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress.OsTPS38-overexpressed transgenic line ox-2 was more sensitive to low temperature.Therefore,OsCYP2 may negatively regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice.These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress. 展开更多
关键词 RICE low temperature OsCYP2 OsTPS38 oscdpk7
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