In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improv...In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants （Solanum tuberosum L. cv. D^siree） were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber- specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUTSZ transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUTSZ transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.展开更多
Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and ...Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1 506bp and 1 608 bp, which encode 502 amino acids and 536 amino acids, respectively. The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains. Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z. In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated. In reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering （DAF） and then sharply declined. The potential physiology functions of these two sucrose transporters are discussed.展开更多
基金supported by the State Key Basic Research and Development Plan of China,the Innovation Foundation of the Chinese Academy of Science,the Program Strategic Scientific Alliances and the China Exchange Program between China and The Netherlands
文摘In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants （Solanum tuberosum L. cv. D^siree） were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber- specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUTSZ transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUTSZ transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.
基金Supported by the State Key Basic Research and Development Plan of China (2004CB720406)Innovation Foundation of the Chinese Academy of Sciences (KSCXZ-SW-306 and KSCX-SW-03)+1 种基金Program Strategic Scientific Alliances (KNAW-PSA, 04-PSA-BD-04 for P.B.F.O.)the China Exchange Program (KNAW-CEP, 04CDp022 for Y.X.) between China and The Netherlands.
文摘Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1 506bp and 1 608 bp, which encode 502 amino acids and 536 amino acids, respectively. The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains. Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z. In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated. In reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering （DAF） and then sharply declined. The potential physiology functions of these two sucrose transporters are discussed.
