Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant ...Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.展开更多
Our previous study revealed that early application of electrical field stimulation（EFS） with the anode at the lesion and the cathode distal to the lesion reduced injury potential, inhibited secondary injury and was ...Our previous study revealed that early application of electrical field stimulation（EFS） with the anode at the lesion and the cathode distal to the lesion reduced injury potential, inhibited secondary injury and was neuroprotective in the dorsal corticospinal tract after spinal cord injury（SCI）. The objective of this study was to further evaluate the effect of EFS on protection of anterior horn motoneurons and their target musculature after SCI and its mechanism. Rats were randomized into three equal groups. The EFS group received EFS for 30 minutes immediately after injury at T_（10）. SCI group rats were only subjected to SCI and sham group rats were only subjected to laminectomy. Luxol fast blue staining demonstrated that spinal cord tissue in the injury center was better protected; cross-sectional area and perimeter of injured tissue were significantly smaller in the EFS group than in the SCI group. Immunofluorescence and transmission electron microscopy showed that the number of spinal cord anterior horn motoneurons was greater and the number of abnormal neurons reduced in the EFS group compared with the SCI group. Wet weight and cross-sectional area of vastus lateralis muscles were smaller in the SCI group to in the sham group. However, EFS improved muscle atrophy and behavioral examination showed that EFS significantly increased the angle in the inclined plane test and Tarlov＇s motor grading score. The above results confirm that early EFS can effectively impede spinal cord anterior horn motoneuron loss, promote motor function recovery and reduce muscle atrophy in rats after SCI.展开更多
本研究旨在应用康奈尔绵羊净碳水化合物和蛋白质体系(Cornell sheep net carbohydrate and protein system,CNCPS-S)的瘤胃降解预测模型预测日粮碳水化合物(CHO)瘤胃有效降解率,并实测验证CNCPS-S模型预测值的准确性。选取3只安装瘤胃...本研究旨在应用康奈尔绵羊净碳水化合物和蛋白质体系(Cornell sheep net carbohydrate and protein system,CNCPS-S)的瘤胃降解预测模型预测日粮碳水化合物(CHO)瘤胃有效降解率,并实测验证CNCPS-S模型预测值的准确性。选取3只安装瘤胃瘘管、体重分别为(38.03±1.56)kg的哈萨克公羊作为试验动物,配制3种精粗比分别为30∶70、35∶65、40∶60的全混合日粮(TMR),利用瘤胃灌注试验和尼龙袋试验实测3种日粮CHO的瘤胃外流速度(Kp)和降解率,同时利用CNCPS-S模型预测3种日粮CHO的Kp与降解率,对CHO的降解率预测值和实测值进行线性回归分析,评价模型预测的准确性。结果表明:3种TMR的瘤胃食糜Kp随精料比例增加而依次升高(P<0.01),分别为3.58%/h、3.83%/h、4.05%/h,据此计算而得的CHO的降解率分别为42.7%、43.1%、42.3%(P>0.05);随精料比例的增加,3种TMR的瘤胃食糜Kp的模型预测值依次升高(P>0.05),分别为1.62%/h、1.63%/h、1.64%/h,CHO瘤胃降解率的预测值分别为39.5%、39.1%、39.6%(P>0.05);3种日粮CHO瘤胃降解率的预测值与实测值之间的平均偏差比较小(≤4 g/100 g CHO),相关性较高(R2≥0.86),均方根误差也较低(RMSE≤1.93 g/100 g CHO)。以上结果说明,CNCPS-S瘤胃降解预测模型对日粮CHO的降解率具有较好的预测能力。展开更多
基金supported by the National Natural Science Foundations of China (31402033, U1603232)the Special Fund for Basic Scientific Research of Institute of Animal Sciences,the Chinese Academy of Agricultural Sciences (2017ywf-zd-11, Y2017JC03)the Agricultural Science and Technology Innovation Program of China (ASTIPIAS01)
文摘Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.
基金supported by the National Natural Science Foundation of China,No.31400717,51577183the Natural Science Foundation of Beijing of China,No.7164317the Youth Innovation Promotion Association CAS,No.2018172
文摘Our previous study revealed that early application of electrical field stimulation（EFS） with the anode at the lesion and the cathode distal to the lesion reduced injury potential, inhibited secondary injury and was neuroprotective in the dorsal corticospinal tract after spinal cord injury（SCI）. The objective of this study was to further evaluate the effect of EFS on protection of anterior horn motoneurons and their target musculature after SCI and its mechanism. Rats were randomized into three equal groups. The EFS group received EFS for 30 minutes immediately after injury at T_（10）. SCI group rats were only subjected to SCI and sham group rats were only subjected to laminectomy. Luxol fast blue staining demonstrated that spinal cord tissue in the injury center was better protected; cross-sectional area and perimeter of injured tissue were significantly smaller in the EFS group than in the SCI group. Immunofluorescence and transmission electron microscopy showed that the number of spinal cord anterior horn motoneurons was greater and the number of abnormal neurons reduced in the EFS group compared with the SCI group. Wet weight and cross-sectional area of vastus lateralis muscles were smaller in the SCI group to in the sham group. However, EFS improved muscle atrophy and behavioral examination showed that EFS significantly increased the angle in the inclined plane test and Tarlov＇s motor grading score. The above results confirm that early EFS can effectively impede spinal cord anterior horn motoneuron loss, promote motor function recovery and reduce muscle atrophy in rats after SCI.
文摘本研究旨在应用康奈尔绵羊净碳水化合物和蛋白质体系(Cornell sheep net carbohydrate and protein system,CNCPS-S)的瘤胃降解预测模型预测日粮碳水化合物(CHO)瘤胃有效降解率,并实测验证CNCPS-S模型预测值的准确性。选取3只安装瘤胃瘘管、体重分别为(38.03±1.56)kg的哈萨克公羊作为试验动物,配制3种精粗比分别为30∶70、35∶65、40∶60的全混合日粮(TMR),利用瘤胃灌注试验和尼龙袋试验实测3种日粮CHO的瘤胃外流速度(Kp)和降解率,同时利用CNCPS-S模型预测3种日粮CHO的Kp与降解率,对CHO的降解率预测值和实测值进行线性回归分析,评价模型预测的准确性。结果表明:3种TMR的瘤胃食糜Kp随精料比例增加而依次升高(P<0.01),分别为3.58%/h、3.83%/h、4.05%/h,据此计算而得的CHO的降解率分别为42.7%、43.1%、42.3%(P>0.05);随精料比例的增加,3种TMR的瘤胃食糜Kp的模型预测值依次升高(P>0.05),分别为1.62%/h、1.63%/h、1.64%/h,CHO瘤胃降解率的预测值分别为39.5%、39.1%、39.6%(P>0.05);3种日粮CHO瘤胃降解率的预测值与实测值之间的平均偏差比较小(≤4 g/100 g CHO),相关性较高(R2≥0.86),均方根误差也较低(RMSE≤1.93 g/100 g CHO)。以上结果说明,CNCPS-S瘤胃降解预测模型对日粮CHO的降解率具有较好的预测能力。
