Diabetes is an urgent health issue characterized by ethnic and regional variations,and is inseparable from the differ-ent dietary habits.It is worthy to note that the incidence of diabetes in Bai nationality has been ...Diabetes is an urgent health issue characterized by ethnic and regional variations,and is inseparable from the differ-ent dietary habits.It is worthy to note that the incidence of diabetes in Bai nationality has been reported to be much lower than Han in China.As a daily vegetable of Bai,the phytochemical and antidiabetic study of Ottelia acuminata var.acuminata had not been carried out.In this study,41 metabolites with diverse diarylheptanoid(six new ones,Otteacumienes A-F),flavone,sesquiterpenoid,coumarin,lignan,polyacetylene,and alkaloid skeletons were charac-terized from O.acuminata var.acuminata.Among them,the racemic nature of 3 was characterized by chiral resolution and calculated ECD methods.The biological study revealed diarylheptanoids showed significantα-glucosidase inhibi-tory activities with 5 as the most effective one(60-fold stronger than acarbose).Molecular docking studies indicated that these structures have different binding cavities with acarbose.This study demonstrated that O.acuminata var.acuminata might correlated with the low incidence diabetes of Bai and the diarylheptanoids may have potential therapeutic value for diabetes mellitus.展开更多
海菜花是我国的特有种质,利用高通量转录组测序技术对海菜花叶片进行测序,共获得clean data reads 26497174条,Q30%达96.19%,GC含量占比50.57%,获得了较高质量的转录组数据。进一步拼接得到了27040个unigene,并进行数据库比对,共计18910...海菜花是我国的特有种质,利用高通量转录组测序技术对海菜花叶片进行测序,共获得clean data reads 26497174条,Q30%达96.19%,GC含量占比50.57%,获得了较高质量的转录组数据。进一步拼接得到了27040个unigene,并进行数据库比对,共计18910(69.93%)条序列得到注释。在NR注释中,与植物同源性最高的为油棕,占比10.92%;在COG、GO、KEGG、SWISS数据库中分别比对到13531、10505、7552、14020个unigene。SSR特征分析中共检测到4217个位点,其中A/T重复占比最高,其次为AG/CT重复类型。该研究对海菜花转录组数据进行分析,获得了大量的基因序列信息和注释信息,为挖掘海菜花的功能基因、遗传多样性分析和分子育种奠定了基础。展开更多
基金supported financially by the Second Tibetan Plateau Scientific Expedition and Research program (2019QZKK0502)State Key Laboratory of Phytochemistry and Plant Resources in West China (E0230211Z1 and P2019-ZZ05).
文摘Diabetes is an urgent health issue characterized by ethnic and regional variations,and is inseparable from the differ-ent dietary habits.It is worthy to note that the incidence of diabetes in Bai nationality has been reported to be much lower than Han in China.As a daily vegetable of Bai,the phytochemical and antidiabetic study of Ottelia acuminata var.acuminata had not been carried out.In this study,41 metabolites with diverse diarylheptanoid(six new ones,Otteacumienes A-F),flavone,sesquiterpenoid,coumarin,lignan,polyacetylene,and alkaloid skeletons were charac-terized from O.acuminata var.acuminata.Among them,the racemic nature of 3 was characterized by chiral resolution and calculated ECD methods.The biological study revealed diarylheptanoids showed significantα-glucosidase inhibi-tory activities with 5 as the most effective one(60-fold stronger than acarbose).Molecular docking studies indicated that these structures have different binding cavities with acarbose.This study demonstrated that O.acuminata var.acuminata might correlated with the low incidence diabetes of Bai and the diarylheptanoids may have potential therapeutic value for diabetes mellitus.
文摘海菜花是我国的特有种质,利用高通量转录组测序技术对海菜花叶片进行测序,共获得clean data reads 26497174条,Q30%达96.19%,GC含量占比50.57%,获得了较高质量的转录组数据。进一步拼接得到了27040个unigene,并进行数据库比对,共计18910(69.93%)条序列得到注释。在NR注释中,与植物同源性最高的为油棕,占比10.92%;在COG、GO、KEGG、SWISS数据库中分别比对到13531、10505、7552、14020个unigene。SSR特征分析中共检测到4217个位点,其中A/T重复占比最高,其次为AG/CT重复类型。该研究对海菜花转录组数据进行分析,获得了大量的基因序列信息和注释信息,为挖掘海菜花的功能基因、遗传多样性分析和分子育种奠定了基础。
