Epidemic management in a measles outbreak in 2023,the return of vaccine-preventable diseases:A single center,retrospective observational study

Objective:To investigate the importance of immunization in preventing measles infection and to determine the most useful laboratory tests for confirmation of measles.Methods:This study included pediatric cases evaluated with a presumed diagnosis of measles between December 2022 and June 2023,at Marmara University Pendik Training and Research Hospital.The effects of vaccination status and underlying disease on the clinical course,treatments,and complications were evaluated.Results:In total,117 patients were enrolled in the study with a median age of 80 months(IQR:32.5-125.0).Twelve patients with contact history were asymptomatic and had an underlying disorder,and intravenous immunoglobulin was given to them for post-exposure prophylaxis.Fifty-one patients had confirmed measles diagnosis.Ribavirin treatment was given to three patients(a newborn,a girl with rhabdomyosarcoma,and a healthy boy)with respiratory distress.Seventy-eight percent of confirmed measles cases were unvaccinated,and all hospitalized cases were unvaccinated or under-vaccinated.Four full-vaccinated children had confirmed measles infection.Measles PCR from nasopharyngeal swabs was negative in all of them,and their diagnosis was established with anti-measles IgM positivity.Conclusions:The measles vaccine is the most effective way to protect from measles and measles-related complications.Although measles can also occur in fully vaccinated patients,the disease is milder than in unvaccinated patients.Using ELISA and RT-PCR tests together may be beneficial in patients with high clinical suspicion for early diagnosis.

Epidemiology of Mpox Outbreaks and Implications for Surveillance in Imo State, Nigeria

Mpox disease is caused by a double-stranded DNA virus, genus Orthopoxvirus of the family Poxviridae. The incubation period is usually 6 to 13 days but can range from 5 to 21 days while symptoms and signs may persist for 2 to 5 weeks. Although, the clinical features are usually less severe when compared to the deadly smallpox, the disease can be fatal with case fatality rate between 1% and 10%. In Imo State, Nigeria, there has been a changing epidemiology of the disease in the last 6 years and the frequency and geographic distribution of cases have progressively increased. This study aims to conduct a review of the disease epidemiology between 2017 and 2023 and implications for surveillance in Imo State. Surveillance data from the Surveillance Outbreak Response and Management System (SORMAS) was extracted between January 2017 and December 2023 across the 27 Local Government Areas (LGAs) of Imo State. A line list of 231 suspected cases was downloaded into an excel template and analyzed using SPSS® version 20 software. Analysis was done using descriptive statistics and associations were tested using Fischer’s exact at 0.05 level of significance. Of the 231 suspected cases, 57.1% (132) were males, 42.9% (99) were females and the modal age group was between the ages of 0 - 4 (32.5%). Eight (8) LGAs (districts) accounted for 71% (n = 164) of all the suspected cases. 21.2% (49) were confirmed positive, 27 males (55.1%) and 22 females (44.9%) (p > 0.05). Modal age group was 20 - 24 (22.4%, n = 11), 18% (9) were children under 14 years, p > 0.05. Case fatality rate was 8% (n = 4). There was no significant association between mortality and age group. Five (5) LGAs accounted for about 60% (29) of all confirmed cases. These LGAs contribute only 20% to the total population in the State. Only 5.6% and 4% of suspected and confirmed cases, respectively, had knowledge of contact with an infectious source. The study described the epidemiology of Mpox outbreaks between 2017 and 2023 and the findings have significant implications on detection and outbreak response activities.

Climate-driven dengue fever outbreaks in Nepal:Trends,challenges,and strategies

Dengue fever(DF)has become a major public health concern in Nepal,with increasing outbreaks in recent years.Transmitted by Aedes mosquitoes,this climate-sensitive viral disease presents a significant challenge for healthcare providers and policymakers.Since 2004,Nepal has experienced a sharp increase in DF cases,peaking in 2022 with 54784 cases and 88 deaths.The surge,driven mainly by serotypes 1,2,and 3,is exacerbated by climate change,which prolongs mosquito breeding seasons due to warmer temperatures and increased rainfall.This trend has even impacted previously unaffected hilly regions.Effective dengue control strategies must focus on climate change adaptation,strengthening healthcare system reinforcement,raising public awareness,and enhancing vector control measures.Government initiatives,like the national dengue control program,play a critical role,but research and community engagement are also vital for prevention and early detection.Integrating climate resilience into public health efforts is essential to reducing the dengue burden in Nepal.

EPS混合土地基-沉箱抗震性能振动台模型试验

采用振动台试验研究了饱和砂土地基(CSS)和EPS混合土地基(CES)两种地基模型下沉箱结构的抗震性能。对比了两种不同地基模型下土体动力响应特征及沉箱结构动力稳定性,探讨了动力作用下沉箱的受力状态及失稳机制,分析了EPS混合土地基对上部沉箱结构地震稳定性的影响。试验结果表明:振动过程中CES工况的超孔压发展速率明显低于CSS,EPS混合土可显著提高模型抗液化性能。墙后填土作用于沉箱的动土压力沿深度方向呈三角形分布,沉箱中部位置动土压力最大。CES工况沉箱动力响应,包括加速度、位移及转动角等均小于CSS,表现出更好的抗震性能。CES沉箱所受动土推力与惯性力存在明显的相位差,有利于提高沉箱结构的稳定性。

碳纤维和EPS颗粒对RPC性能的影响

研究了不同掺量(0、0.2%、0.3%、0.4%、0.5%、1.0%)的碳纤维(CF)全取代钢纤维(SF)对活性粉末混凝土(RPC)工作性的影响,优选出了适宜CF掺量;在此基础上,研究了EPS颗粒掺量(10%、20%、30%、40%、50%)对掺CF的RPC(CF-RPC)工作性、力学性能、干表观密度和导热系数的影响,并与同EPS颗粒掺量条件下掺SF的RPC(SF-RPC)性能进行了对比。结果表明:CF的适宜掺量为0.3%;随着EPS颗粒掺量的增加,CF-RPC试件的扩展度先增大后减小,抗压强度、抗折强度、干表观密度和导热系数均降低,韧性提升;与掺EPS的SF-RPC试件相比,掺EPS的CF-RPC试件的抗压强度较低,但韧性较好,干表观密度和导热系数均较低。

EPS外墙外保温系统聚氨酯粘结胶加固后受力性能试验研究

通过EPS保温板抗拉试验、疲劳试验、加固疲劳试验,研究粘贴率、粘贴方式对EPS板及聚氨酯粘结胶加固试样的抗拉承载力和疲劳性能影响。结果表明:除满粘外,在相同条件下,EPS板抗拉承载力平均值由大到小为:条粘>点粘>十字粘>点框粘;EPS板疲劳次数与粘贴率呈正相关,条粘法疲劳性能最好;采用聚氨酯粘结胶加固后的试样抗拉承载力平均值均符合要求,且达到满粘抗拉承载力的90%;初始疲劳对加固效果的影响最大仅7%,加固后点粘、条粘、十字粘、点框粘的疲劳次数分别达到满粘的87%、89%、87%、75%;聚氨酯粘结胶加固后累计疲劳次数更多,EPS板整体使用寿命得到延长,加固方法较为可靠。

EPS混凝土的早龄期抗冲击性能

对早龄期(12 h、24 h、36 h)的EPS混凝土进行了动态力学试验,从动态抗压强度、峰值应变、极限应变和能耗密度等方面分析了冲击速度(4.5~6.5 m/s)和龄期(12 h、24 h、36 h)对EPS混凝土抗冲击性能的影响,并与28 d龄期时EPS混凝土的性能进行了对比。结果表明:EPS混凝土的动态抗压强度、峰值应变、极限应变和能耗密度均具有冲击速度强化效应。随着龄期的增大,EPS混凝土的动态力学性能指标及其对冲击速度的敏感性不断增大。28 d龄期时,EPS混凝土的动态抗压强度、峰值应变、极限应变和能耗密度最大,其对冲击速度的敏感性最强。EPS混凝土早龄期具有良好的变形和吸能特性。36 h龄期时,EPS混凝土的峰值应变、极限应变和能耗密度分别可达到28 d龄期时的66%~82%、91%~93%和72%~78%。

BF型钢EPS泡沫混凝土外挂墙板有限元分析

为研究玄武岩纤维对型钢EPS泡沫混凝土预制外挂墙板的作用影响,本文通过棱柱体试验以及相关文献获得不同掺量玄武岩纤维的EPS泡沫混凝土的本构曲线,利用有限元软件ABAQUS对预制墙板进行有限元分析,探究其不同掺量时对EPS轻骨料混凝土力学性能的影响规律。研究结果表明:当玄武岩纤维掺量为0.2%时,对构件承载力有明显提升;而当玄武岩纤维掺量为0.6%时,对构件承载力几乎没有提升,且延性有所下降。因此,最佳掺量为2%。此外,冷弯薄壁C型钢与EPS泡沫混凝土之间具有较高黏结强度,且掺入玄武岩纤维不改变墙板的破坏模式。

基于CLSI指南EP09-A3比对2套化学发光免疫分析系统血清TT_3测定结果

目的 基于美国临床实验室标准化协会(CLSI)指南EP09-A3探讨2套化学发光免疫分析系统血清总三碘甲状腺原氨酸(TT_3)测定结果的可比性。方法 根据EP09-A3指南要求,使用2个品牌化学发光免疫分析系统(分别命名为系统一和系统二)分别测定111例血清样本TT_3水平。通过广义极端学生化偏差(ESD)法检验离群值,选用EP09-A3指南列举的5种回归模型分析2套系统TT_3检测结果,并计算医学决定水平处的偏移。结果 散点图目测和ESD法检验均未发现离群值。2套系统TT_3检测结果的5种回归分析结果显示,一般线性回归、加权最小二乘法回归、Deming回归、Passing-Bablock回归分析在TT_3医学决定水平处(0.75和1.91 ng·mL~(-1))的偏移均<可接受范围[允许总误差(TEa)±12.5%],偏移的95%可信区间(CI)结果类型解读为可接受的A型和B型,加权Deming回归分析得到的偏移的95%CI部分超出可接受范围,结果类型解读为C型。结论 EP09-A3指南适用于2套系统检测TT_3的方法学比对和偏移评估,且该指南更新的离群值判断方法、回归分类分析法和对应的解读分型方式更为科学、合理。

调脾增食颗粒对幼龄厌食大鼠血浆β-EP及CCK-8的影响

目的:探讨调脾增食颗粒对幼龄厌食大鼠血浆β-EP(β-内腓肽)及CCK-8(胆囊收缩素)的影响。方法:选取60只幼龄SD大鼠,随机分为6组,正常对照组,模型对照组,调脾增食颗粒高、中、低剂量组及阳性对照组,每组10只。除正常对照组喂饲常规鼠饲料外,其余各组均采用特制高蛋白高脂饲料喂养1周,建立幼龄厌食大鼠模型。正常对照组和模型对照组给予蒸馏水,调脾增食颗粒高、中、低剂量组按33.86、16.93、8.47 g/(kg·d)剂量给药,阳性组给予健胃消食片0.675 g/(kg·d),连续给药4周;记录各组大鼠进食量,应用放射免疫法测定大鼠血浆β-EP及CCK-8的含量。结果:调脾增食颗粒中、高剂量组第3周和第4周的进食量明显多于模型对照组(P<0.05,P<0.01)。调脾增食颗粒中、高剂量组大鼠血浆β-EP含量明显高于模型组(P<0.05,P<0.01),CCK-8含量明显低于模型组(P<0.05,P<0.01)。结论:调脾增食颗粒治疗厌食症与调节血β-EP及CCK-8的分泌与释放有关。

H2 strain attenuated live hepatitis A vaccines:protective efficacy in a hepatitis A outbreak

AIM:To investigate the protective efficacy of H2 strain attenuated live hepatitis A vaccines (H2-strain vaccines) in hepatitis A (HA) outbreaks.METHODS:With the permission of their parents, 5551 pre-school and grade 1-3 primary school children were inoculated with 1 dose (10(6.5) TCID(50)) of H2 strain vaccines in a nonrandomized, controlled trial conducted in Fucheng County, Hebei Province in May 1997.Another 6485 children in the same grades and compatible in gender and age were enrolled as controls. Epidemiological and serological survey was conducted to evaluate the protective efficacy of the vaccines. ELISA was used to detect serum IgM anti-HAV.RESULTS:HA outbreak started in early May 1998, peaked in the middle of the same month, and lasted about 80 days. Overall 302 HA cases were found, 192(63.58%) were 5-9 years old. One vaccinee and 25 control cases were found to have hepatitis A, which account for 0.28% (1/356) and 5.92% (25/422) of all vaccinees and controls in the 14 villages, respectively. The protective efficacy of vaccines was 95.27% (95% CI: 85.83%-104.72%). In subjects tested for anti-HAV IgM from 13 villages, 1(0.40%) overt and 11(4.06%) asymptomatic HAV cases were found in 271 vaccinees but 21(6.69%) of overt and asymptomatic ones were found in 314 controls.CONCLUSION:H2 strain vaccines were excellent in preventing overt hepatitis A,but not so effective in preventing asymptomatic hepatitis A virus infection.A booster dose might be needed to get permanent reliable immunity.

Epidemiological Survey and Analysis on an Outbreak of Gastroenteritis due to Water Contamination

Objective To document the investigation and control of an outbreak of gastroenteritis in City G, South China, and provide a reference for preventing future outbreaks. Methods An ambispective cohort study was designed. Attack rate （AR） and relative risks （RR） were calculated to identify the causes of gastroenteritis. Investigations using questionnaires included personal interviews with patients and doctors, reviews of medical records, laboratory examinations of fecal specimens and continuous hygiene monitoring of water samples from the waterworks. Results Overall, 427/71534 （AR=5.97%o） cases were identified between October 31 and November 12 2010. Geographic distribution was highly localized, with 80% of cases occurring in the areas supplied by waterworks-A. Consumption of water provided solely by waterworks-A was found to be associated with illness （RR=8.20, 95 C1%：6.12-10.99） compared with that from waterworks-B. Microbiological analyses confirmed the presence of Norovirus in six of eight fecal samples from symptomatic patients, two water samples from waterworks-A and two sewage samples. After taking effective measures, the hygienic indices of waterworks-A met health criteria again on November 9 and no cases were reported 3 days later.Conclusion The outbreak reported here was caused by drinking tap water contaminated with sewage at the source. Early identification of possible contamination sources and awareness of changes that might negatively impact water quality are important preventive measures to protect public health.

Epidemiological Characteristics and Spatiotemporal Distribution Patterns of Human Norovirus Outbreaks in China, 2012–2018

Objective To clarify the epidemiological characteristics and spatial distribution patterns of human norovirus outbreaks in China, identify high-risk areas, and provide guidance for epidemic prevention and control.Methods This study analyzed 964 human norovirus outbreaks involving 50,548 cases in 26 provinces reported from 2012 to 2018. Epidemiological analysis and spatiotemporal scanning analysis were conducted to analyze the distribution of norovirus outbreaks in China.Results The outbreaks showed typical seasonality, with more outbreaks in winter and fewer in summer, and the total number of infected cases increased over time. Schools, especially middle schools and primary schools, are the most common settings of norovirus outbreaks, with the major transmission route being life contact. More outbreaks occurred in southeast coastal areas in China and showed significant spatial aggregation. The highly clustered areas of norovirus outbreaks have expanded northeast over time.Conclusion By identifying the epidemiological characteristics and high-risk areas of norovirus outbreaks, this study provides important scientific support for the development of preventive and control measures for norovirus outbreaks, which is conducive to the administrative management of high-risk settings and reduction of disease burden in susceptible areas.

Epidemiological Characteristics of An Outbreak of Severe Acute Respiratory Syndrome in Dongcheng District of Beijing From March to May 2003

Objective To describe epidemiologic features of an outbreak of severe acute respiratory syndrome (SARS) in Dongcheng District, Beijing occurred in a period between March and May 2003. Methods Data of SARS cases notified from Dongcheng District Center for Disease Control and Prevention (CDC )and supplemented by other channels were collected. Clinicians and officials of local hospitals were interviewed in groups and medical records of fatal cases of SARS were reviewed to verify the diagnosis. Stored serum specimens of the patients were detected for IgG antibody against SARS Co-V by enzyme-linked immunosorbent assay (ELISA). All the data were input into dataset files by Microsoft Excel-2000 software and analyzed with SPSS version 10.0 software. Results Outbreak of SARS in Dongcheng District started on March 14, 2003 with a peak in mid- and late April, and dropped in early May. A total of 572 reported cases were collected during this period in Dongcheng District, Beijing, and 99 of them were excluded from SARS, because of diagnosis of common cold, regular pneumonia, measles and rubella, etc. Actually, 473 SARS cases, which included 390 (82.5%) probable cases and 83 (17.5%) suspect cases, were analyzed. About 90% of the probable cases were positive for IgG antibody. Attack rate of SARS in permanent residents of Dongcheng District was 28.3 per 100 000. Forty-one of them died, with a case-fatality rate of 8.7%. Persons were all susceptible to infection of SARS Co-V, with the highest proportion at ages of 20-50 years, which accounted for 68.7% of the total cases. Average age of the patients at their onset was 40.7 years. No gender difference in SARS cases was found. Number of SARS cases in health-care workers (HCWs) accounted for 18.0% and that in retired workers accounted for 15.4% of the total cases. Cases occurred in all 10 sub-districts of Dongcheng, with the highest in Beixinqiao and Andingmen Sub-districts. Totally, 230 of the 572 notified cases (40.2%) were hospitalized at local hospitals under the jurisdiction of Dongcheng District. Eighteen of 85 cases of SARS occurred in HCWs of local hospitals, accounting for 4.5% of the total number of HCWs working at wards caring for SARS patients or fever clinics. There were 34.7% of SARS cases without any histories of contact before the onset of the disease. Familial aggregation phenomena were observed in 41.8% of the cases and 18.1% of households. And 7.4% (attack rate ) of those exposed to SARS cases suffered from the illness during the periods of quarantine. Conclusions SARS appeared to be infectious in origin and caused outbreak in Dongcheng District, Beijing introduced by an imported case traveling from Hong Kong in a period between March and May 2003. People are all susceptible to infection of SARS Co-V, which mainly threatens the young adults and the middle-aged, as well as HCWs and the retired workers. The main mode of transmission is direct exposure to SARS patients in a near distance at hospitals or families via droplets spread. Prevention and control of SARS should be focused on early isolation of patients and quarantine for close contacts. Current available measures to prevent and control SARS are proved to be effective.

Outcome of Epidemiological Investigation of COVID-19 Outbreak in a South West State of Nigeria, March to August 2020

Background: On 3rd April 2020, an outbreak of Coronavirus disease-2019 (COVID-19) was confirmed in Ondo State, Southwest Nigeria. Field investigations were conducted by the State Ministry of Health (MoH) to identify and confirm additional cases. This paper provides the outcome of the epidemiological investigation of the outbreak to further guide outbreak response activities. Methods: Field epidemiology methods coordinated by the State Public Health Emergency Operations Center (PHEOC) were used in the outbreak investigation. Suspected cases were reported through the routine Integrated Disease Surveillance and Response (IDSR) network in the State. Also, active case search/finding to communities and health facilities using the operational case definitions was conducted. Furthermore, the state COVID-19 emergency call center was established with toll-free phone number provided to members of the public to call-in to report suspected persons with symptoms consistent with the COVID-19 operational case definition (signal). All cases reported were investigated with nasopharyngeal sample collected and confirmatory testing for COVID-19 done by real-time polymerase chain reaction (RT-PCR) in a national reference laboratory. Information of each case investigated was documented using the COVID-19 case investigation form and COVID-19 line-list which captured socio-demographic characteristics, history of exposure to COVID-19, presenting symptoms, travel history and sample collection details. Data were extracted from the case investigation form and line list and analyzed using SPSS version 20, with a p-value set at < 0.05. Results: A total of 4353 suspected cases were reported and investigated, of which 1316 cases were confirmed between March 19, 2020 and August 9, 2020. The mean age of the confirmed cases was 37.8 ± 14.8 years. The highest proportion of COVID-19 cases and deaths occurred in the age group 20 - 39 years (50.6%) and ≥60 years (13.5%), respectively. More males (52.4%) were affected than females (45.8%). There were 404 (30.7%) healthcare workers infection. About a quarter (325;24.7%) of the cases was symptomatic and presented with key symptoms such as cough (98/325;30.2%) and fever (74/325;22.8%) during the investigation. A case fatality rate (CFR) of 2.2% was recorded in 7 of the 17 Local Government Areas (LGAs) where confirmed cases were reported in the State. Most of the confirmed cases, 1169 (88.8%) reside in urban LGAs of the State. The epidemic curve shows a propagated pattern of transmission with the outbreak reaching its peak on July 2, 2020. Significantly, higher proportion of death was recorded in the age group ≥ 60 years (14;13.5%) and among males (26;3.6%) (p < 0.001). Conclusions: Our investigation found high transmission of COVID-19 infection among urban residents (LGAs) and health care workers with high mortality among males in older age group in the State. Hence, we recommend intensified risk communication and use of community structures to ensure compliance with public health COVID-19 preventive protocols including enforcing infection prevention and control compliance, particularly among high-risk groups.

Public health preparedness towards COVID-19 outbreak in Nigeria

The 2019 novel coronavirus disease(COVID-19),formerly called novel Coronavirus(2019-nCoV),was discovered in December 2019.This outbreak was declared a public health emergency of international concern(PHEIC)on 30th January 2020 by the World Health Organization(WHO).The WHO announced US$675 million fund to strengthen China and nations with weaker health systems in combating COVID-19.By 18th February 2020,there has been 73332 confirmed cases with one case in Egypt[1].

Protective effect of inactivated hepatitis A vaccine against the outbreak of hepatitis A in an open rural community

AIM： To evaluate the protective effect of inactivated hepatitis A vaccine （Healive） against hepatitis A outbreak in an emergency vaccination campaign. METHODS： During an outbreak of hepatitis A in Honghe Town, Xiuzhou District, Jiaxing City, Zhejiang Province, two nonrandomized controlled trials were conducted in September 2006. The first trial was to vaccinate 108 anti-HAV negative individuals with close contacts of the patients from September with 1 dose of an inactivated hepatYds A vaccine, HeaUve. The control group comprised of 115 individuals with close contacts of the patients before September. The second trial was to vaccinate 3365 primary and secondary school students who volunteered to receive a dose of Healive and 2572 students who did not receive Healive serving as its controls. An epidemiological survey was conducted to evaluate the pmtectk, e efficacy of the vaccine. RESULTS： A total of 136 hepatitis A cases were reported during an outbreak that started in June, peaked in August and September, and ended after December of 2006. After a massive vaccination of school children in September, the number of cases declined significantly. No hepatitis A was detected in the 108 vaccinated individuals with dose contacts of patients, whereas 4 cases of hepatitis A were found in the controls. The infection rate of hepatitis A was not significantly different in the individuals with close contacts of patients whether or not they received the vaccine （P = 0.122）. No hepatitis A was detected in the 3365 students who received the vaccine, four cases of hepatitis A were found in the controls. The infection rate of students with or without vaccination was significantly diffeent in the students who received the vaccine （0/3365 vs 4/2572, P = 0.035）. The protective efficacy of the vaccine was 100%.CONCLUSION： Inactivated hepatitis A vaccine demonstrates a good protective effect against an outbreak of hepatitis A.

An Outbreak of Leptospirosis in Lezhi County, China in 2010 May Possibly Be Linked to Rainfall

A total of 26 leptospirosis cases occurred in the outbreak, of whom six died after hospitalization. All 26 patients were clinically diagnosed as leptospirosis Microscopic agglutination test （MAT） revealed that 13 of 26 patients were affected with laboratory confirmed leptospirosis. Furthermore, MAT data suggested that serogroup Icterohaemorrhagiae were the main pathogens responsible for this outbreak. And the rainfall data suggested that the increased rainfall might be responsible for the leptospirosis outbreak in Lezhi County. This outbreak has reminded us that leptospirosis should not be neglected, especially during flood season, although its incidence rate is low.

EP300对口腔鳞癌细胞增殖和迁移的影响

目的 探讨EP300在口腔鳞癌中的表达水平以及对口腔鳞癌细胞增殖、迁移的影响。方法 通过在线数据库收集并分析EP300基因在泛癌组织及正常组织中的表达差异。采用qRT-PCR和Western blot分别检测EP300在正常口腔黏膜上皮细胞株NOK和两种不同OSCC细胞株HSC3、UM1中的基因和蛋白表达水平;通过质粒转染沉默HSC3和UM1细胞株中EP300基因,平板克隆和CCK-8检测细胞增殖能力,划痕实验检测细胞迁移能力。结果 生信分析发现EP300在头颈部鳞癌HNSCC中的表达较癌旁组织显著上调;与NOK组比较,HSC3和UM1中EP300基因和蛋白表达水平显著增高(P<0.05),且HSC3和UM1两种细胞系的EP300内源性表达相对更高。CCK-8增殖实验和克隆形成实验检测发现,沉默EP300后HSC3和UM1的增殖能力明显降低(P<0.05);细胞划痕实验检测发现沉默EP300后HSC3和UM1的迁移能力明显降低(P<0.05)。结论 EP300在口腔鳞癌细胞株HSC3和UM1中高表达,EP300低表达能够抑制口腔鳞癌细胞的增殖和迁移。

Combined cesarean delivery and repair of acute aortic dissection at 34 weeks of pregnancy during COVID-19 outbreak:A case report

BACKGROUND Since the outbreak of the coronavirus disease 2019(COVID-19)pandemic,the exclusion of a patient from COVID-19 should be performed before surgery.However,patients with type A acute aortic dissection(AAD)during pregnancy can seriously endanger the health of either the mother or fetus that requires emergency surgical treatment without the test for COVID-19.CASE SUMMARY A 38-year-old woman without Marfan syndrome was admitted to the hospital because of chest pain in the 34th week of gestation.She has diagnosed as having a Stanford type-A AAD involving an aortic arch and descending aorta via aortic computed tomographic angiography.The patient was transferred to the isolated negative pressure operating room in one hour and underwent cesarean delivery and ascending aorta replacement.All medical staff adopted third-level medical protection measures throughout the patient transfer and surgical procedure.After surgery,the patient was transferred to the isolated negative pressure intensive care unit ward.The nucleic acid test and anti-COVID-19 immunoglobulin(Ig)G and IgM were performed and were negative.The patient and infant were discharged without complication nine days later and recovered uneventfully.CONCLUSION The results indicated that the procedure that we used is feasible in patients with a combined cesarean delivery and surgery for Stanford type-A AAD during the COVID-19 outbreak,which was mainly attributed to rapid multidisciplinary consultation,collaboration,and quick decision-making.