Erratum to:Vibration analysis of a gear-rotor-bearing system with outer-ring spalling and misalignment

Because of an unfortunate mistake during the production of this article,Figure 13 was wrongly inserted.The corrected Figure 13 is shown as follows.

Analysis and optimization of stamping and forming process of bearing outer ring

To address the common issues of wrinkling,tearing,and uneven wall thickness in the actual sheet metal stamp-ing process of the outer ring of needle roller bearings,this study analyzes critical technical indicators such asforming limits,thickness distribution,and principal strains in the forming process in detail.Three-dimensionalmodels of the concave and convex dies were constructed.The effects of different process parameters,includingstamping speed,edge pressure,sheet metal thickness,and friction coefficient,on the quality of the forming partswere investigated by varying these parameters.Subsequently,the orthogonal experimental method was used todetermine an optimal experimental group from multiple sets of experiments.It was found that under the processparameters of a stamping speed of 3000 mm/s,edge pressure of 2000 N,sheet metal thickness of 0.9 mm,andfriction coefficient of 0.125,the forming quality of the outer ring of the bearing is ideal.

Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain unclear.Therefore,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under microgravity.After 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic cells.To investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail suspension.The results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly upregulated.In addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated weightlessness.These findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

Pepper mild mottle virus coat protein interacts with pepper chloroplast outer envelope membrane protein OMP24 to inhibit antiviral immunity in plants

Pepper mild mottle virus(PMMoV)is a devastating viral pathogen of pepper(Capsicum annuum)but it is unclear whether and how peppers protect against PMMoV infection.The expression of the chloroplast outer membrane protein 24(OMP24)of C.annuum was upregulated under PMMoV infection and it interacted with PMMoV coat protein(CP).Silencing of OMP24 in either C.annuum or Nicotiana benthamiana facilitated PMMoV infection,whereas overexpression of N.benthamiana OMP24 in transgenic plants inhibited PMMoV infection.Both C.annuum OMP24(CaOMP24)and N.benthamiana OMP24(NbOMP24)localized to the chloroplast and have a moderately hydrophobic transmembrane domain that is necessary for their localization.Overexpression of CaOMP24 induced stromules,perinuclear chloroplast clustering,and accumulation of reactive oxygen species(ROS),the typical defense responses of chloroplasts transferring the retrograde signaling to the nucleus to regulate resistance genes.The expression of PR1 and PR2 was also upregulated significantly in plants overexpressing OMP24.Self-interaction of OMP24 was demonstrated and was required for OMP24-mediated plant defense.Interaction with PMMoV CP interfered with the self-interaction of OMP24 and impaired OMP24-induced stromules,perinuclear chloroplast clustering and ROS accumulation.The results demonstrate the defense function of OMP24 in pepper during viral infection and suggest a possible mechanism by which PMMoV CP modulates the plant defense to facilitate viral infection.

A data assimilation-based forecast model of outer radiation belt electron fluxes

Because radiation belt electrons can pose a potential threat to the safety of satellites orbiting in space,it is of great importance to develop a reliable model that can predict the highly dynamic variations in outer radiation belt electron fluxes.In the present study,we develop a forecast model of radiation belt electron fluxes based on the data assimilation method,in terms of Van Allen Probe measurements combined with three-dimensional radiation belt numerical simulations.Our forecast model can cover the entire outer radiation belt with a high temporal resolution(1 hour)and a spatial resolution of 0.25 L over a wide range of both electron energy(0.1-5.0 MeV)and pitch angle(5°-90°).On the basis of this model,we forecast hourly electron fluxes for the next 1,2,and 3 days during an intense geomagnetic storm and evaluate the corresponding prediction performance.Our model can reasonably predict the stormtime evolution of radiation belt electrons with high prediction efficiency(up to~0.8-1).The best prediction performance is found for~0.3-3 MeV electrons at L=~3.25-4.5,which extends to higher L and lower energies with increasing pitch angle.Our results demonstrate that the forecast model developed can be a powerful tool to predict the spatiotemporal changes in outer radiation belt electron fluxes,and the model has both scientific significance and practical implications.

Newly Detected Transmission of bla_(KPC-2) by Outer Membrane Vesicles in Klebsiella Pneumoniae

Objective The prevalence of carbapenem-resistant Klebsiella pneumoniae(CR-KP)is a global public health problem.It is mainly caused by the plasmid-carried carbapenemase gene.Outer membrane vesicles(OMVs)contain toxins and other factors involved in various biological processes,includingβ-lactamase and antibiotic-resistance genes.This study aimed to reveal the transmission mechanism of OMV-mediated drug resistance of Klebsiella(K.)pneumoniae.Methods We selected CR-KP producing K.pneumoniae carbapenemase-2(KPC-2)to study whether they can transfer resistance genes through OMVs.The OMVs of CR-KP were obtained by ultracentrifugation,and incubated with carbapenem-sensitive K.pneumoniae for 4 h.Finally,the carbapenem-sensitive K.pneumoniae was tested for the presence of bla_(KPC-2)resistance gene and its sensitivity to carbapenem antibiotics.Results The existence of OMVs was observed by the electron microscopy.The extracted OMVs had bla_(KPC-2)resistance gene.After incubation with OMVs,bla_(KPC-2)resistance gene was detected in sensitive K.pneumoniae,and it became resistant to imipenem and meropenem.Conclusion This study demonstrated that OMVs isolated from KPC-2-producing CR-KP could deliver bla_(KPC-2)to sensitive K.pneumoniae,allowing the bacteria to produce carbapenemase,which may provide a novel target for innovative therapies in combination with conventional antibiotics for treating carbapenem-resistant Enterobacteriaceae.

A Lagrangian Trajectory Analysis of Azimuthally Asymmetric Equivalent Potential Temperature in the Outer Core of Sheared Tropical Cyclones

In this study,the characteristics of azimuthally asymmetric equivalent potential temperature(θ_(e))distributions in the outer core of tropical cyclones(TCs)encountering weak and strong vertical wind shear are examined using a Lagrangian trajectory method.Evaporatively forced downdrafts in the outer rainbands can transport low-entropy air downward,resulting in the lowestθ_(e)in the downshear-left boundary layer.Quantitative estimations ofθ_(e)recovery indicate that air parcels,especially those originating from the downshear-left outer core,can gradually revive from a low entropy state through surface enthalpy fluxes as the parcels move cyclonically.As a result,the maximumθ_(e)is observed in the downshear-right quadrant of a highly sheared TC.The trajectory analyses also indicate that parcels that move upward in the outer rainbands and those that travel through the inner core due to shear make a dominant contribution to the midlevel enhancement ofθ_(e)in the downshear-left outer core.In particular,the former plays a leading role in suchθ_(e)enhancements,while the latter plays a secondary role.As a result,moist potential stability occurs in the middle-to-lower troposphere in the downshear-left outer core.

Proteomic and Functional Analyses of Outer Membrane Vesicles Secreted by Vibrio splendidus

Vibrio splendidus is an important opportunistic pathogen ubiquitously present in the marine environment,exhibiting virulence to a variety of cultured animals.The extracellular products secreted by V.splendidus are crucial to bacterial survival and virulence.In this study,the secretion of outer membrane vesicles(OMVs)by V.splendidus was determined,purified,and morphologically characterized.The protein composition of OMVs was analyzed by proteomic analysis.The results showed that approximately 120 proteins were contained in these OMVs,including outer membrane proteins,flagellins,ABC transporters,protease,and iron regulation proteins,etc.,which were involved in bacterial motility,formation of biofilms and the cell membrane components,and cellular localization based on their structural molecule activity,passive transmembrane transporter activity,channel activity,neurotransmitter receptor activity,extracellular ligand-gated ion channel activity,glutamate receptor activity,ligand-gated ion channel activity,and transmembrane signaling receptor activity.To explore the biological functions of OMVs in V.splendidus,the effects of OMVs on the bacterial adaption to iron limitation,antibiotic,and the coelomic fluid of the Apostichopus japonicus were confirmed.This study is the first time to show that V.splendidus secretes OMVs,and OMVs carry functional proteins that enhance bacterial survival under various stresses.

On-Site Assessment of a Cryogenic Disinfectant for the Alpine Environment and Outer Packaging of Frozen Items

Objective To study the effectiveness and feasibility of cryogenic disinfectants in different cold scenarios and analyze the key points of on-site cryogenic disinfection.Methods Qingdao and Suifenhe were selected as application sites for the manual or mechanical spraying of cryogenic disinfectants.The same amount of disinfectant(3,000 mg/L)was applied on cold chain food packaging,cold chain containers,transport vehicles,alpine environments,and article surfaces.The killing log value of the cryogenic disinfectant against the indicator microorganisms(Staphylococcus aureus and Escherichia coli)was used to evaluate the on-site disinfection effect.Results When using 3,000 mg/L with an action time of 10 min on the ground in alpine regions,the surface of frozen items,cold-chain containers,and cold chain food packaging in supermarkets,all external surfaces were successfully disinfected,with a pass rate of 100%.The disinfection pass rates for cold chain food packaging and cold chain transport vehicles of centralized supervised warehouses and food processing enterprises were 12.5%(15/120),81.67%(49/60),and 93.33%(14/15),respectively;yet,the surfaces were not fully sprayed.Conclusion Cryogenic disinfectants are effective in disinfecting alpine environments and the outer packaging of frozen items.The application of cryogenic disinfectants should be regulated to ensure that they cover all surfaces of the disinfected object,thus ensuring effective cryogenic disinfection.

Effect of High-Speed Solar Winds Turbulence Upstream of the Earth’s Magnetosphere: Case of the Outer Minima of Solar Cycles 20, 21, 22, 23 and 24

Highly turbulent environment, the solar wind is a stream of very energetic particles mainly made of protons and electrons. During its trip in the interplanetary space, this solar flow becomes more accelerated during the outer minima (descending phases) of the solar cycles and can therefore influence all of humanity and its technology. These disturbances lead to socio-economic consequences requiring a precise knowledge of the climate variability. Using a statistical approach, we evaluate the response of the Earth’s magnetosphere to the High-Speed Solar Winds (HSSW) forcing during the peaks of the last five outer minima. To do so, 1UA data of solar wind and magnetic field parameters were extracted from OMNI browser. Analysis of the energetic solar plasma particles shows that strong geomagnetic field variations can occur even in the absence of large solar disturbances. While the normalized reconnection rate was estimated to be ~21% of the total variance of the magnetospheric variables, the upstream of the magnetic cavity was perturbed 80% of the time with large energies recorded. As a result, Earth’s magnetosphere becomes denser (i.e., more drag), which is a problem for spacecraft. Thus, the coupled solar wind-magnetosphere system follows scale-invariant dynamics and is in a state far from equilibrium. Our analysis provides insight into the main cause of geomagnetic storms with more than 97% of HSSW imposed in the range 300 - 850 km/s. These high-speeds lead to auroras that can disrupt electrical and communication systems.

高含水复杂断块油藏单层系边外注水开发模式

复杂断块油藏在开发后期面临综合含水率高、地层能量不足、剩余油分布复杂、井网适应性差等问题。以济阳坳陷沾化凹陷中部垦71断块油藏为例,基于水驱油藏的油包水和水包油2种油水分布状态,利用理论模型推导和数值模拟方法,开展了单层系边外注水开发模式研究。结果表明:从采液端和注水端的受力分析可获得单层系开发的合理注水量、合理采液量和合理注采井距,单层系开发的合理注水压差、合理注水量、合理采液量是影响其剩余油饱和度、含水饱和度的关键因素,对油层合理地层压力水平及剩余油二次富集起主要控制作用。通过加大注采井距至油水边界外围,以2.9倍含油条带宽度为合理注采井距的临界值,采用短注长静置的非对称周期注采方式,建立单层系边外注水开发模式,有利于保持单层系的压力系统稳定和提高剩余油波及率,从而提高原油采收率。矿场实践表明,采用单层系边外注水开发模式后,垦71断块油藏日产油量提高至254 t/d,含水率控制在93.7%,采收率提高至31.53%,采油速率提高4.9%,显示出良好的增产效果。单层系边外注水开发模式解决了高含水复杂断块油藏开发中的剩余油分布分散、水驱波及率低和井网适应性差等难题,可为复杂断块油藏开发提供指导和借鉴。

内圣外王的期盼:中国古代君德论及其特征

中国古代君德论起源于殷周,发展于两汉,兴盛于唐,繁荣于两宋,变革于明末清初,一脉相承而内容不断丰富。君德的内容包含了君主的德性、器识、才智、能力等诸多因素,主要体现在君主个人修养以及施政过程中如何处理君臣关系、君民关系等方面。完善君德的方式主要有经筵讲读、皇储教育、家训教化、大臣谏诤、史书褒贬。古代君德论特征鲜明,君主地位的尊崇、历史的教训与现实的需要是君德论的起点,儒家“修齐治平”“内圣外王”理论是君德论的思想基础,读经观史,以经为则、以史为鉴是完善君德的重要手段,“致君尧舜”是君德修养的重点。古人已经形成了一套围绕君主而展开的关于君德修养的理论体系。同时也要看到,古人讨论君德,既存在“师道”与“君道”之间无法调和的矛盾,也没有进行制度层面的反思,反映的只是一种泛道德化的政治伦理观。

“内国”与“外土”:周代铜器铭文所见的两个疆域概念

周代铜器录卣铭中的“内国”及戎生钟铭中的“外土”构成了一对在内涵上密切相关的疆域概念。这两个概念有特定内涵,“内国”意为“内域”,在铭文中指以郑洛地区为中心的中原腹地,这一地区自夏代至两周一直是早期王朝的核心疆域。“外土”为周王朝领土的外层区域,其地存在着大量的土著族群。不过作为周王朝能够有效管辖的区域,该区仍属周王朝之疆域。“内国”与“外土”作为一对疆域概念,反映了周王朝疆域形态及疆域治理均具有多层次性的特征。“内国”是王朝疆域的内层,由王廷所任命的内服职官加以治理。“外土”则是王朝疆域的外层,周王朝于此广设侯、伯贵族以治理土著族群并威慑在其之外的敌对势力。至于“外土”之外的区域,亦可能受到周人的军事打击,但周王朝已无法对其进行有效控制,其地已非周之疆域。

外空安全与环境治理:进展、挑战与机遇

随着人类航天活动的迅猛发展,外空环境日益复杂无序,外空安全的威胁显著上升,外层空间长期可持续性面临严重挑战。本文全面介绍了外空安全面临的最新态势,系统分析了空间碎片数量逐年增多、低轨大规模星座迅猛部署、新型航天活动快速喷发、外空资源争夺日趋激烈、外空军事化愈演愈烈等对外空安全和外空长期可持续的冲击。本文指出,从外空命运共同体、国家空间资产安全、航天强国建设出发,外空环境治理是大势所趋和唯一出路,孕育着航天技术创新、产业革命、法规建制等重大机遇。基于国情提出了我国对策和发展建议。

载人航天商业化的国际损害责任制度:困境与应对

载人航天商业化的发展需要平衡外层空间的公共性和商业载人航天的私营性,国际损害责任制度对管控商业载人航天的成本和风险至关重要。国际外空法对国际损害责任进行了一般性、原则性规定,部分航天国家在国内法中对商业载人航天的损害责任予以明确。基于外空领域的现有责任规则,构建国际损害责任制度面临着责任主体和索赔主体不明、过失作为例外要件存在不合理、国际保险标准难统一、责任分配制度待完善的困境。对此,可考虑通过关联性确认责任主体和索赔主体、对损害统一适用绝对责任、引入强制责任保险、形成双重责任制度,为国家载人航天商业化发展提供制度构建思路;同时,促进国际社会达成共识,推动人类命运共同体理念在外空领域的进一步实现。

面向物流仓储的基于边缘计算的高价值工业制品外包装表面破损识别研究

高价值工业制品具有重要的经济和战略价值,其物流仓储过程中的破损和损失不仅会带来高昂的直接和间接成本,还会对生产和供应链产生严重的影响。其中,及时发现和处理高价值工业制品外包装表面的破损可以避免货物继续损坏。边缘计算可以通过利用物联网、图像处理、人工智能等先进技术,对货物外包装表面破损情况进行实时监测和预警,从而大幅提高物流仓储的安全性和效率。文章面向物流仓储,基于边缘计算对高价值工业制品外包装表面破损识别展开研究,以期为构建现代化物流和仓储体系、提高高价值工业制品的物流运输质量提供一定参考。

用户需求驱动下的女性口红外包装设计路径研究

目的针对我国女性口红外包装存在的问题,提出一种集成Kano-AHP-TOPSIS模型的口红外包装设计路径。方法首先,以口红外包装为研究对象,通过对女性消费者进行多渠道调研,成功获取十六项口红外包装用户需求,并运用Kano模型对其进行属性划分;其次,引入AHP法构建用户需求层次分析模型,通过运算求解各层级需求权重,提出三款口红外包装设计方案;最后,运用TOPSIS法对三款设计方案及评价样本进行综合评价,结果表明,基于Kano-AHP-TOPSISI所构思的三款口红外包装设计均优于现有的口红外包装设计。结论集成Kano-AHP-TOPSIS模型在女性口红外包装研发过程中可合理划分用户需求属性、科学求解各属性需求权重、客观评价设计方案,该设计路径指导女性口红外包装设计具有可行性,可为同类女性化妆品包装设计提供参考和思路。

鲍曼不动杆菌OmpA蛋白的生物学特性分析

目的提取临床分离的鲍曼不动杆菌外膜蛋白A(OmpA)的基因,并进行生物学预测和分析。方法查询鲍曼不动杆菌OmpA基因序列,设计特异性PCR引物,以提取的鲍曼不动杆菌基因组DNA为模板,PCR扩增OmpA片段。回收目的片段,采用生物信息学软件分析OmpA蛋白的生物学特性。结果12株鲍曼不动杆菌多位点序列分型(MLST)序列分析有6个ST分型,分别是ST 208、ST 229、ST 191、ST 195、ST540、ST 1145,鲍曼不动杆菌OmpA基因序列全长为1070 bp的,单核苷酸多态性(SNP)位点较少,预测蛋白为跨膜蛋白,具有6种三级结构,均由β-桶状结构组成的保守结构域。结论临床分离的不同鲍曼不动杆菌株OmpA蛋白为结构相对保守的跨膜蛋白,具有维持细胞的形状和稳定性,参与细菌耐药机制及免疫原性作用。

月球站所的法律地位浅析

目前,月球活动正进入新的阶段——月球站所的筹备与建设。然而,现有相关国际法规范尚需完善,其中月球站所的法律地位是需首要考虑的问题。对月球站所法律地位问题的思考始于月球站所活动的特殊性:以人类社会的可持续发展为长远目标,开展于天体之上,将对天体表面固定区域进行使用且产生事实上的活动范围。合乎《外空条约》的月球站所建设是为全人类谋福利的自由探索和利用月球的活动,不存在既排他又永久地使用月球表面区域的情形,且在互惠基础上对他国开放。为此,建议在国际月球科研站活动中考虑“非排他”和“有时限”二者择一的规范路径以履行相应的国际法义务,并且明确国际月球科研站对他国开放的“互惠”前提与“参观”范畴。同时,需将月球站所的四个方面作为判断其法律地位的具体考量因素,即月球站所的使用目的与活动范围大小,月球站所的可移动性,月球站所的使用期限以及月球站所信息的通知、更新与公开。

考虑外圈变形和轴承座支承作用的球轴承动力学研究

高速、重载或薄壁球轴承等情况下的外圈结构弹性变形和柔性外圈与轴承座的配合支承特性对轴承及其系统的动力学性能有着重要的影响。采用等效刚体单元和Timoshenko梁单元建立了柔性外圈等效力学模型,考虑钢球与柔性外圈的分段滚道之间、柔性外圈与轴承座之间的动态接触作用,建立了计及外圈结构弹性变形和轴承座支承作用的球轴承多体接触动力学模型。运用广义-α方法计算分析了外圈弹性变形和轴承座支承作用对轴承载荷分布规律和动态配合特性的影响规律,获得了不同配合间隙、内部游隙、外圈厚度和径向力等因素下球轴承的动态作用力、载荷分布角和相对位移等动力学结果。结果表明:外圈增加至一定厚度,球轴承内部载荷分布与刚性套圈理论具有很好的一致性,刚性外圈与刚性轴承座的支承接触区域相对较小;随着外圈壁厚的减小,柔性外圈与轴承座的支承接触力逐渐由平滑曲线变为典型的多点极值和非光滑分布的动态变化规律,且相邻极值的差值,随着外圈壁厚的减小,配合间隙、内部游隙和径向力的增加而明显增加;随着配合间隙或内部游隙增大,球轴承载荷分布范围和轴承座的支承接触区域总体上呈现减小趋势;不同的配合间隙、内部游隙或径向载荷下薄壁外圈更容易发生近似对称的压缩、扩张、拉伸或压扁等不同形式的结构弹性变形。提出的动力学模型为滚动轴承及其系统的动力学研究和动态设计提供新方法。