Importance of developing alternative energy● We shall take alternative energy as a foreseeable,strategic and long-range issue, and pay great attention to it from the angle of national strategy.
How the scientific outlook toward developmem is related to human rights in China was the theme of a forum held in Beijing by the Human Rights magazine on April 18. Attending were a dozen experts from the Chinese Acade...How the scientific outlook toward developmem is related to human rights in China was the theme of a forum held in Beijing by the Human Rights magazine on April 18. Attending were a dozen experts from the Chinese Academy of Social Sciences, the Central Party School, Beijing University, Renmin University of China, China Political Science and Law University, PLA Airforce Command Institute and China Society for Human Rights Studies. They discussed a range of questions including the connotation of the scientific outlook toward development, how the scientific outlook toward development is related to human rights and how it guides the development of human rights in both theory and practice, and the significance of the inclusion of human rights into China's Eleventh Five-Year Program for Economic and Social Development (2006-2010).展开更多
During the 12^(th)Five-Year Plan period,the environmental protection information work made remarkable achievements,and the construction of nationwide air quality monitoring network was completed; the trinitarian monit...During the 12^(th)Five-Year Plan period,the environmental protection information work made remarkable achievements,and the construction of nationwide air quality monitoring network was completed; the trinitarian monitoring system was established,and environmental emergency command ability enhanced significantly. However,the issues of " information island" and " application silo" are still outstanding,and the application and promotion of new technologies make slow progress; the capacity of information system to support environmental protection business is insufficient,and there is no capacity for decision analysis. In the 13^(th)Five-Year Plan period,it is needed to strengthen the application and promotion of new technologies such as cloud computing,big data,Internet of Things,and spatial information,mainly construct and improve " Cloud Computing Based Environmental Protection( CCBEP) ", " big data platform of ecological environment",and then realize the core objective of improving environmental quality.展开更多
The scientific outlook on development is a practical demonstration of the dialectics,which is based on all-faceted,coordinated and perpetual laws,and perfect representation of universal truth and fundamental principles.
Over the past decade,China had developed rapidly in the natural gas industry and become one of the largest natural gas countries in the world.From 2009 to 2019,natural gas consumption in China had been growing rapidly...Over the past decade,China had developed rapidly in the natural gas industry and become one of the largest natural gas countries in the world.From 2009 to 2019,natural gas consumption in China had been growing rapidly with a compound annual growth rate of 13.1%,making it the world’s third largest consumer of natural gas now.The share of natural gas in primary energy consumption mix is constantly increasing and reaches 8.6%in 2020.Natural gas proved reserves in China grow at an compound annual growth rate of 8.9%during the past decade.展开更多
During the past decade,China’s petroleum industry had experienced an extraordinary process,the development of domestic oil supply and demand was unbalanced.China’s oil consumption continues to grow with a compound a...During the past decade,China’s petroleum industry had experienced an extraordinary process,the development of domestic oil supply and demand was unbalanced.China’s oil consumption continues to grow with a compound annual growth rate of 5.1%,making China the world’s second largest oil consumer,the share of oil in primary energy consumption mix increased steadily and accounted for 20%in 2019.Oil proved reserves increased grow at a low rate with a compound annual growth rate of 2.1%from 2008 to 2018.China’s proved reserve ranked the 14th in the world in 2019.Oil production increased and exceeded 200 million in 2010,reached the historical peak in 2015,and then continued to decline from 2016 to 2018.China’s Oil imports are growing rapidly and its import dependency has continued to rise.In 2017,China surpassed the United States to become the world’s largest oil importer.In the past decade,the import dependency had increased by more than 20%and reached nearly 72%in 2019.展开更多
This paper aims to interpret the connotation of high-quality development of tourism economy(HQTE)from the perspective of the new development concepts of innovation,coordination,green,openness and sharing,and then to e...This paper aims to interpret the connotation of high-quality development of tourism economy(HQTE)from the perspective of the new development concepts of innovation,coordination,green,openness and sharing,and then to evaluate the spatial differenti-ation of China’s HQTE based on provincial panel data from 2009 to 2018.Specifically,we employ the spatial convergence model to ex-plore the absolute and conditionalβconvergence trends of HQTE in the whole country and the eastern,central and western regions of China.Our empirical results reveal that:1)within the decade,from 2009 to 2018,regions of China with the highest HQTE index is its eastern region followed by the central region and then the western region,but the fastest growing one is the western region of China fol-lowed by the central region and then the eastern region.2)Whether or not the spatial effect is included,there are absolute and condition-alβconvergence in HQTE in the whole country and aforementioned three regions.3)The degree of government attention as well as the level of economic development and location accessibility are the positive driving factors for the convergence of HQTE in the whole country and the three regions.The degree of marketization and human capital have not passed the significance test either in the whole country or in the three regions.The above conclusions could deepen the understanding of the regional imbalance and spatial conver-gence characteristics of HQTE,clarify the primary development objects,and accomplish the goal of China’s HQTE.展开更多
The Historical Resultant Theory and The Scientific Outlook on Development are consistent in essence,with the former serving as the philosophical foundation of the latter,and the latter being the new and modern form of...The Historical Resultant Theory and The Scientific Outlook on Development are consistent in essence,with the former serving as the philosophical foundation of the latter,and the latter being the new and modern form of the former.Thorough analysis and deep understanding of the dialectical relationship of the two theories contributes a lot to promoting China's localization of Marxism and to building a harmonious socialist society,both theoretically and practically.展开更多
The purpose of the research was to assess the impact of Citizen Development activities on digital transformation. The research identified eight categories that contribute to the success of Low-code No-code (LCNC) proj...The purpose of the research was to assess the impact of Citizen Development activities on digital transformation. The research identified eight categories that contribute to the success of Low-code No-code (LCNC) projects: 1) Strategy;2) Infrastructure;3) Technology;4) Processes & Procedures;5) Governance;6) Culture;7) People;8) Goals & Metrics and selected six critical success factors from these categories: 1) Operational Efficiency;2) Time Savings;3) Timeframe to Realize Value;4) Employee Engagement;5) Participation;6) Number of Sponsored Ideas. End users of the digital transformation efforts through Citizen Development were asked to assess the six critical success measures in terms of performance and importance criteria. The research results identified that focus should be applied to improving “Timeframe to Realize Value”, on “Operational Efficiency”, and on “Time Savings” to deliver success.展开更多
Enterprises must develop and implement effective strategic measures to achieve sustainable development.This entails evaluating corporate performance,understanding the impact of sustainable development strategies on co...Enterprises must develop and implement effective strategic measures to achieve sustainable development.This entails evaluating corporate performance,understanding the impact of sustainable development strategies on corporate performance,and implementing such strategies.The article proposes eight critical countermeasures to foster harmony and sustainable development among humanity,nature,the economy,and society:innovation-driven initiatives,green development practices,social responsibility initiatives,talent cultivation programs,collaborative cooperation efforts,sound financial management,brand-building endeavors,and effective information management.展开更多
Since the beginning of the United Nations Decade of Education for Sustainable Development(DESD)(2005-2014)in 2005,Education for Sustainable Development(ESD)has been started a new era of educational innovation progress...Since the beginning of the United Nations Decade of Education for Sustainable Development(DESD)(2005-2014)in 2005,Education for Sustainable Development(ESD)has been started a new era of educational innovation progress in China.Up to now,China has created a new history of ESD for 21 years,and ESD,the abstract conception from international academia has been became a leading thought of teaching and learning innovation for more and more teachers in China,and in 2010,ESD was been written in the Outline of the National Program for Medium and Long-term Education Reform and Development(2010-2020)by Chinese Ministry of Education as a strategic direction of education development for the coming ten years.Exactly,ESD has really played a positive role in educational reforni and innovation.展开更多
Commercial shale gas production has been rapidly realized in China following a short learning period.Shale gas production is expected to reach 4.5bcm by the end of 2015,with the construction of a 7.5 ban production ca...Commercial shale gas production has been rapidly realized in China following a short learning period.Shale gas production is expected to reach 4.5bcm by the end of 2015,with the construction of a 7.5 ban production capacity.Over 700 shale gas wells have been drilled in China,mostly by the three state-owned oil companies.The most significant factors that led to the rapid shale gas development in China include the abundant shale gas resources,a relatively complete R&D system,and strong support from various stakeholders(the central and local governments,oil companies,and the general public).China has a long way to go for building a booming shale gas industry,because of many big obstacles in the way of shale gas development,including complex geology,high operation costs,incomplete infrastructure and technology constraints.Looking ahead,however,there are firm grounds for the projection of a bright prospect of China's shale gas industry,as a 20 bcm annual shale gas production is reasonably expected in 2020.展开更多
Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lili...Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily.展开更多
Roof plate secretion of bone morphogenetic proteins(BMPs)directs the cellular fate of sensory neurons during spinal cord development,including the formation of the ascending sensory columns,though their biology is not...Roof plate secretion of bone morphogenetic proteins(BMPs)directs the cellular fate of sensory neurons during spinal cord development,including the formation of the ascending sensory columns,though their biology is not well understood.Type-ⅡBMP receptor(BMPRⅡ),the cognate receptor,is expressed by neural precursor cells during embryogenesis;however,an in vitro method of enriching BMPRⅡ^(+)human neural precursor cells(hNPCs)from the fetal spinal cord is absent.Immunofluorescence was undertaken on intact second-trimester human fetal spinal cord using antibodies to BMPRⅡand leukemia inhibitory factor(LIF).Regions of highest BMPRⅡ^(+)immunofluorescence localized to sensory columns.Parenchymal and meningeal-associated BMPRⅡ^(+)vascular cells were identified in both intact fetal spinal cord and cortex by co-positivity with vascular lineage markers,CD34/CD39.LIF immunostaining identified a population of somas concentrated in dorsal and ventral horn interneurons,mirroring the expression of LIF receptor/CD118.A combination of LIF supplementation and high-density culture maintained culture growth beyond 10 passages,while synergistically increasing the proportion of neurospheres with a stratified,cytoarchitecture.These neurospheres were characterized by BMPRⅡ^(+)/MAP2ab^(+/–)/βⅢ-tubulin^(+)/nestin^(–)/vimentin^(–)/GFAP^(–)/NeuN^(–)surface hNPCs surrounding a heterogeneous core ofβⅢ-tubulin^(+)/nestin^(+)/vimentin^(+)/GFAP^(+)/MAP2ab^(–)/NeuN^(–)multipotent precursors.Dissociated cultures from tripotential neurospheres contained neuronal(βⅢ-tubulin^(+)),astrocytic(GFAP+),and oligodendrocytic(O4+)lineage cells.Fluorescence-activated cell sorting-sorted BMPRⅡ^(+)hNPCs were MAP2ab^(+/–)/βⅢ-tubulin^(+)/GFAP^(–)/O4^(–)in culture.This is the first isolation of BMPRⅡ^(+)hNPCs identified and characterized in human fetal spinal cords.Our data show that LIF combines synergistically with high-density reaggregate cultures to support the organotypic reorganization of neurospheres,characterized by surface BMPRⅡ^(+)hNPCs.Our study has provided a new methodology for an in vitro model capable of amplifying human fetal spinal cord cell numbers for>10 passages.Investigations of the role BMPRⅡplays in spinal cord development have primarily relied upon mouse and rat models,with interpolations to human development being derived through inference.Because of significant species differences between murine biology and human,including anatomical dissimilarities in central nervous system(CNS)structure,the findings made in murine models cannot be presumed to apply to human spinal cord development.For these reasons,our human in vitro model offers a novel tool to better understand neurodevelopmental pathways,including BMP signaling,as well as spinal cord injury research and testing drug therapies.展开更多
Since the formal establishment of diplomatic relations in 1979,China and Djibouti have made significant progress in developing bilateral relations,with the two countries supporting and cooperating with each other and ...Since the formal establishment of diplomatic relations in 1979,China and Djibouti have made significant progress in developing bilateral relations,with the two countries supporting and cooperating with each other and the two peoples sharing a strong bond.Especially since the 18th National Congress of the Communist Party of China,with the joint efforts of China and Djibouti,the bilateral relations have enjoyed comprehensive and rapid development.展开更多
文摘Importance of developing alternative energy● We shall take alternative energy as a foreseeable,strategic and long-range issue, and pay great attention to it from the angle of national strategy.
文摘How the scientific outlook toward developmem is related to human rights in China was the theme of a forum held in Beijing by the Human Rights magazine on April 18. Attending were a dozen experts from the Chinese Academy of Social Sciences, the Central Party School, Beijing University, Renmin University of China, China Political Science and Law University, PLA Airforce Command Institute and China Society for Human Rights Studies. They discussed a range of questions including the connotation of the scientific outlook toward development, how the scientific outlook toward development is related to human rights and how it guides the development of human rights in both theory and practice, and the significance of the inclusion of human rights into China's Eleventh Five-Year Program for Economic and Social Development (2006-2010).
文摘During the 12^(th)Five-Year Plan period,the environmental protection information work made remarkable achievements,and the construction of nationwide air quality monitoring network was completed; the trinitarian monitoring system was established,and environmental emergency command ability enhanced significantly. However,the issues of " information island" and " application silo" are still outstanding,and the application and promotion of new technologies make slow progress; the capacity of information system to support environmental protection business is insufficient,and there is no capacity for decision analysis. In the 13^(th)Five-Year Plan period,it is needed to strengthen the application and promotion of new technologies such as cloud computing,big data,Internet of Things,and spatial information,mainly construct and improve " Cloud Computing Based Environmental Protection( CCBEP) ", " big data platform of ecological environment",and then realize the core objective of improving environmental quality.
文摘The scientific outlook on development is a practical demonstration of the dialectics,which is based on all-faceted,coordinated and perpetual laws,and perfect representation of universal truth and fundamental principles.
文摘Over the past decade,China had developed rapidly in the natural gas industry and become one of the largest natural gas countries in the world.From 2009 to 2019,natural gas consumption in China had been growing rapidly with a compound annual growth rate of 13.1%,making it the world’s third largest consumer of natural gas now.The share of natural gas in primary energy consumption mix is constantly increasing and reaches 8.6%in 2020.Natural gas proved reserves in China grow at an compound annual growth rate of 8.9%during the past decade.
文摘During the past decade,China’s petroleum industry had experienced an extraordinary process,the development of domestic oil supply and demand was unbalanced.China’s oil consumption continues to grow with a compound annual growth rate of 5.1%,making China the world’s second largest oil consumer,the share of oil in primary energy consumption mix increased steadily and accounted for 20%in 2019.Oil proved reserves increased grow at a low rate with a compound annual growth rate of 2.1%from 2008 to 2018.China’s proved reserve ranked the 14th in the world in 2019.Oil production increased and exceeded 200 million in 2010,reached the historical peak in 2015,and then continued to decline from 2016 to 2018.China’s Oil imports are growing rapidly and its import dependency has continued to rise.In 2017,China surpassed the United States to become the world’s largest oil importer.In the past decade,the import dependency had increased by more than 20%and reached nearly 72%in 2019.
基金Under the auspices of the National Natural Science Foundation of China(No.42001156)。
文摘This paper aims to interpret the connotation of high-quality development of tourism economy(HQTE)from the perspective of the new development concepts of innovation,coordination,green,openness and sharing,and then to evaluate the spatial differenti-ation of China’s HQTE based on provincial panel data from 2009 to 2018.Specifically,we employ the spatial convergence model to ex-plore the absolute and conditionalβconvergence trends of HQTE in the whole country and the eastern,central and western regions of China.Our empirical results reveal that:1)within the decade,from 2009 to 2018,regions of China with the highest HQTE index is its eastern region followed by the central region and then the western region,but the fastest growing one is the western region of China fol-lowed by the central region and then the eastern region.2)Whether or not the spatial effect is included,there are absolute and condition-alβconvergence in HQTE in the whole country and aforementioned three regions.3)The degree of government attention as well as the level of economic development and location accessibility are the positive driving factors for the convergence of HQTE in the whole country and the three regions.The degree of marketization and human capital have not passed the significance test either in the whole country or in the three regions.The above conclusions could deepen the understanding of the regional imbalance and spatial conver-gence characteristics of HQTE,clarify the primary development objects,and accomplish the goal of China’s HQTE.
文摘The Historical Resultant Theory and The Scientific Outlook on Development are consistent in essence,with the former serving as the philosophical foundation of the latter,and the latter being the new and modern form of the former.Thorough analysis and deep understanding of the dialectical relationship of the two theories contributes a lot to promoting China's localization of Marxism and to building a harmonious socialist society,both theoretically and practically.
基金Fund Project: the National Natural Science Foundation of China (No: 70673075) the National Social Science Foundation of China (No.: 07BJV035)+1 种基金 the Nation Education & Science 11th Five-Year Plan--Ministry of Education, youth issues (No.: EFA090413) the Hubei Province Science & Technology Department, special issues of soft science (No.: 2009DEA049).
文摘The purpose of the research was to assess the impact of Citizen Development activities on digital transformation. The research identified eight categories that contribute to the success of Low-code No-code (LCNC) projects: 1) Strategy;2) Infrastructure;3) Technology;4) Processes & Procedures;5) Governance;6) Culture;7) People;8) Goals & Metrics and selected six critical success factors from these categories: 1) Operational Efficiency;2) Time Savings;3) Timeframe to Realize Value;4) Employee Engagement;5) Participation;6) Number of Sponsored Ideas. End users of the digital transformation efforts through Citizen Development were asked to assess the six critical success measures in terms of performance and importance criteria. The research results identified that focus should be applied to improving “Timeframe to Realize Value”, on “Operational Efficiency”, and on “Time Savings” to deliver success.
文摘Enterprises must develop and implement effective strategic measures to achieve sustainable development.This entails evaluating corporate performance,understanding the impact of sustainable development strategies on corporate performance,and implementing such strategies.The article proposes eight critical countermeasures to foster harmony and sustainable development among humanity,nature,the economy,and society:innovation-driven initiatives,green development practices,social responsibility initiatives,talent cultivation programs,collaborative cooperation efforts,sound financial management,brand-building endeavors,and effective information management.
文摘Since the beginning of the United Nations Decade of Education for Sustainable Development(DESD)(2005-2014)in 2005,Education for Sustainable Development(ESD)has been started a new era of educational innovation progress in China.Up to now,China has created a new history of ESD for 21 years,and ESD,the abstract conception from international academia has been became a leading thought of teaching and learning innovation for more and more teachers in China,and in 2010,ESD was been written in the Outline of the National Program for Medium and Long-term Education Reform and Development(2010-2020)by Chinese Ministry of Education as a strategic direction of education development for the coming ten years.Exactly,ESD has really played a positive role in educational reforni and innovation.
文摘Commercial shale gas production has been rapidly realized in China following a short learning period.Shale gas production is expected to reach 4.5bcm by the end of 2015,with the construction of a 7.5 ban production capacity.Over 700 shale gas wells have been drilled in China,mostly by the three state-owned oil companies.The most significant factors that led to the rapid shale gas development in China include the abundant shale gas resources,a relatively complete R&D system,and strong support from various stakeholders(the central and local governments,oil companies,and the general public).China has a long way to go for building a booming shale gas industry,because of many big obstacles in the way of shale gas development,including complex geology,high operation costs,incomplete infrastructure and technology constraints.Looking ahead,however,there are firm grounds for the projection of a bright prospect of China's shale gas industry,as a 20 bcm annual shale gas production is reasonably expected in 2020.
基金supported by the National Key Research and Development Program of China(2022YFD1200500)the Fundamental Research Funds for the Central Universities(KYZZ2022004)+1 种基金the Project for Crop Germplasm Resources Conservation of Jiangsu(2021-SJ-011)the High Level Talent Project of the Top Six Talents in Jiangsu(NY-077)。
文摘Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily.
基金supported by grants from the National Health and Medical Research Council(NHMRC)of Australia(Nos.571100 and 1048082)the Baxter Charitable Foundation(to TCL)+1 种基金Medical Research grants from the Rebecca L.Cooper Medical Research Foundation(to MWW,TCL,and MDL)supported by a Charles D.Kelman,M.D.Postdoctoral Award(2010)from the International Retinal Research Foundation(USA)。
文摘Roof plate secretion of bone morphogenetic proteins(BMPs)directs the cellular fate of sensory neurons during spinal cord development,including the formation of the ascending sensory columns,though their biology is not well understood.Type-ⅡBMP receptor(BMPRⅡ),the cognate receptor,is expressed by neural precursor cells during embryogenesis;however,an in vitro method of enriching BMPRⅡ^(+)human neural precursor cells(hNPCs)from the fetal spinal cord is absent.Immunofluorescence was undertaken on intact second-trimester human fetal spinal cord using antibodies to BMPRⅡand leukemia inhibitory factor(LIF).Regions of highest BMPRⅡ^(+)immunofluorescence localized to sensory columns.Parenchymal and meningeal-associated BMPRⅡ^(+)vascular cells were identified in both intact fetal spinal cord and cortex by co-positivity with vascular lineage markers,CD34/CD39.LIF immunostaining identified a population of somas concentrated in dorsal and ventral horn interneurons,mirroring the expression of LIF receptor/CD118.A combination of LIF supplementation and high-density culture maintained culture growth beyond 10 passages,while synergistically increasing the proportion of neurospheres with a stratified,cytoarchitecture.These neurospheres were characterized by BMPRⅡ^(+)/MAP2ab^(+/–)/βⅢ-tubulin^(+)/nestin^(–)/vimentin^(–)/GFAP^(–)/NeuN^(–)surface hNPCs surrounding a heterogeneous core ofβⅢ-tubulin^(+)/nestin^(+)/vimentin^(+)/GFAP^(+)/MAP2ab^(–)/NeuN^(–)multipotent precursors.Dissociated cultures from tripotential neurospheres contained neuronal(βⅢ-tubulin^(+)),astrocytic(GFAP+),and oligodendrocytic(O4+)lineage cells.Fluorescence-activated cell sorting-sorted BMPRⅡ^(+)hNPCs were MAP2ab^(+/–)/βⅢ-tubulin^(+)/GFAP^(–)/O4^(–)in culture.This is the first isolation of BMPRⅡ^(+)hNPCs identified and characterized in human fetal spinal cords.Our data show that LIF combines synergistically with high-density reaggregate cultures to support the organotypic reorganization of neurospheres,characterized by surface BMPRⅡ^(+)hNPCs.Our study has provided a new methodology for an in vitro model capable of amplifying human fetal spinal cord cell numbers for>10 passages.Investigations of the role BMPRⅡplays in spinal cord development have primarily relied upon mouse and rat models,with interpolations to human development being derived through inference.Because of significant species differences between murine biology and human,including anatomical dissimilarities in central nervous system(CNS)structure,the findings made in murine models cannot be presumed to apply to human spinal cord development.For these reasons,our human in vitro model offers a novel tool to better understand neurodevelopmental pathways,including BMP signaling,as well as spinal cord injury research and testing drug therapies.
文摘Since the formal establishment of diplomatic relations in 1979,China and Djibouti have made significant progress in developing bilateral relations,with the two countries supporting and cooperating with each other and the two peoples sharing a strong bond.Especially since the 18th National Congress of the Communist Party of China,with the joint efforts of China and Djibouti,the bilateral relations have enjoyed comprehensive and rapid development.