Effect of resveratrol on pancreatic oxygen free radicals in rats with severe acute pancreatitis

瞄准：在试验性的严重尖锐胰腺炎(树液) 上作为自由基 scavenger 调查 resveratrol (RESV ) 的治疗学的效果。方法：72 只男 Sprague-Dawley 老鼠随机被划分成假冒的操作组，树液组，和对待 resveratrol 的组。胰腺炎被 0.1 mL/kg 的管内管理导致 4% 牛磺胆酸钠。RESV 在 20 mg/kg 体重的剂量静脉内地被给。所有动物在 3 点被打死， 6，在模型的正式就职以后的 12 h。浆液淀粉酶，胰腺的超级氧化物歧化酶(草皮) ， malondialdehyde (MDA ) ，和 myeloperoxidase (军邮局) 是坚定的。胰的病理学的变化在光显微镜下面被观察。结果：浆液淀粉酶，胰腺的军邮局和病理学的损坏的分数在胰腺炎的正式就职以后增加了，早(3， 6 h ) 树液样品被减少的胰腺的草皮描绘并且增加了胰腺的 MDA。Resveratrol 在氧自由基在树液的早阶段引起的房间膜每氧化对类脂化合物展出了保护的效果。氧化还原作用状态缺陷的这变细减少了细胞的氧化损坏，由更低的浆液淀粉酶思考了不太严重的胰腺的损害，正常胰腺的 MDA 层次，以及在胰的减少的嗜中性的渗入。结论：RESV 可以由降低胰腺的氧化自由基并且减少 neutrophils 的胰腺的织物渗入在树液上施加它的治疗学的效果。

Granulocyte colony-stimulating factor increases extracellular glutamic acid uptake and suppresses free radicals in an experimental model of amyotrophic lateral sclerosis

Excitatory amino acid toxicity and free radical damage play important roles in amyotrophic lateral sclerosis. Granulocyte colony-stimulating factor （G-CSF） protects nerve cells exposed to high-concentrations of glutamic acid, suggesting positive effects in the treatment of amyotrophic lateral sclerosis. The present study induced in vitro motor neuron injury using glutamic acid excitotoxicity, and the biochemical effects of G-CSF on glutamic acid concentration were determined. In addition, the effects of G-CSF on superoxide dismutase, glutathione peroxidase activity in motor neurons, and malondialdehyde and nitric oxide contents were analyzed. Immunohistochemistry was performed to measure neuronal survival. Results revealed that G-CSF significantly suppressed free radical activity, inhibited excitotoxicity, and reduced apoptosis and loss of motor neurons in the anterior horn of the spinal cord.

Effects of ulinastatin combined with surgery on nerve injury, oxygen free radicals and inflammatory factors production of patients with hypertensive intracerebral hemorrhage

Objective: To study the effects of ulinastatin combined with surgery on nerve injury, oxygen free radicals and inflammatory factors production of patients with hypertensive intracerebral hemorrhage. Methods: The patients with hypertensive intracerebral hemorrhage received surgical treatment in our hospital during February 2015 - December 2017 were selected and divided into two groups by random number table. The observation group received ulinastatin combined surgery and the control group received conventional medicine combined with surgery. Before treatment and 1 weeks after treatment, serum levels of nerve damage markers, neurotrophic indexes, oxygen free radicals production indexes and inflammatory cytokines were measured. Results: After treatment, the contents of NSE, VILIP-1, GFAP, S100B, MDA, AOPP, 8-OHdG, NO, ET-1, TNF-α, sICAM-1, sVCAM-1, sICAM-1 of two groups were all decreased, the contents of BDNF, NGF, VEGF, IGF-I were all increased and the decreasing trend of NSE, VILIP-1, GFAP, S100B, MDA, AOPP, 8-OHdG, NO, ET-1, TNF-α, sICAM-1, sVCAM-1, sICAM-1 of observation group was more obvious than that of the control group, the increasing trend of BDNF, NGF, VEGF, IGF-I content was more obvious than that of the control group. Conclusion: The use of ulinastatin combined with surgery in the treatment of hypertensive intracerebral hemorrhage can significantly reduce the damage of nerve function and reduce the production of oxygen free radicals and inflammatory factors.

Correlation of oxygen free radical items between blood and kidney of chronic renal failure rat

Ｃｏｒｒｅｌａｔｉｏｎｏｆｏｘｙｇｅｎｆｒｅｅｒａｄｉｃａｌｉｔｅｍｓｂｅｔｗｅｅｎｂｌｏｏｄａｎｄｋｉｄｎｅｙｏｆｃｈｒｏｎｉｃｒｅｎａｌｆａｉｌｕｒｅｒａｔ￥ＹｕａｎＦａｈｕａｎ（袁发焕）；ＬｉａｏＬｉｓｈｅｎｇ（廖立生）（Ｄｅｐａｒｔｍｅｎｔｏ...

Effect of Selenium Poisoning on Immune Function and Oxygen Free Radicals in Erythrocyte of Pig

[ Objective] This study was to investigate the effect of selenium（Se） on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divided into three groups, two testing groups and one control group. For each group, Se content in whole blood, immune function of erythrocytes, activity of whole blood glutathione peroxidase（GSH-Px）, activity of blood plasma superoxide dismutase（SOD）, and blood plasma malondialdehyde（MDA） content were determined. [ Result] Compared with the control group, Se content in whole blood and blood plasma MDA content increased remarkably, while whole blood GSH-Px activity and blood plasma SOD activity decreased; RBC-C1 RR assumed a rise-fall trend, and RBC-ICR showed no obvious change. [Condusion] Se poisoning can reduce the activity of antioxidant enzymes, disturb the balance of oxygen free radicals metabolism, thereby inducing erythrooyte immune function in piglets.

Correlation of electrophysiological characteristics Tp-ec and Tp-e/QT with myocardial injury and oxygen free radical generation in patients with acute myocardial infarction

Objective: To study the correlation of electrophysiological characteristics Tp-ec and Tp-e/QT with myocardial injury and oxygen free radical generation in patients with acute myocardial infarction. Methods: Patients who were diagnosed with acute myocardial infarction in our hospital between March 2014 and March 2017 were selected as the AMI group, and healthy subjects who received physical examination were selected as the control group, electrocardiography was done to determine Tp-ec and Tp-e/QT, serum was collected to determine the levels of myocardial injury markers, oxygen free radical generation indexes and apoptosis indexes, and peripheral blood was collected to determine the expression of oxygen free radical generation indexes. Results: Tp-ec and Tp-e/QT of AMI group were significantly higher than those of control group, and serum CK-MB, cTnI, H-FABP, MDA, sTWEAK, sFas and sTRAIL contents as well as peripheral blood Nrf-2, NRE and HO-1 mRNA expression were significantly higher than those of control group and positively correlated with Tp-ec and Tp-e/QT. Conclusion: Electrophysiological characteristics Tp-ec and Tp-e/QT increase, and the transseptal dispersion of repolarization increases in patients with acute myocardial infarction, and they are closely related to myocardial injury and oxygen free radical generation.

卵巢型子宫内膜异位症患者外周血ROS、PON-1水平和miR-20a表达水平及与病情的关系

目的探究卵巢型子宫内膜异位症(EMs)患者外周血氧自由基(ROS)、氧磷酶-1(PON-1)水平和miR-20a表达水平及与病情的关系。方法回顾性选取2020年3月至2023年3月在江苏省人民医院(南京医科大学第一附属医院)进行治疗的113例卵巢型EMs患者纳入研究组,并根据美国生育协会分期标准对其进行分组:62例Ⅰ~Ⅱ期患者纳入轻度组,51例Ⅲ~Ⅳ期患者纳入重度组。另选30例在本院进行治疗的盆腔炎且子宫内膜正常患者纳入对照组。比较研究组与对照组患者、轻度组与重度组患者的ROS、PON-1水平和miR-20a相对表达水平,比较轻度组与重度组的血清炎症因子[白细胞介素(IL)-6、IL-8、单核细胞趋化蛋白-1(MCP-1)、肿瘤坏死因子-α(TNF-α)]水平,并探究其与患者病情严重程度的相关性,采用受试者工作特征(ROC)曲线分析ROS、PON-1水平和miR-20a表达水平对重度卵巢型EMs的诊断价值。结果研究组患者的ROS水平和miR-20a相对表达水平分别为(485.55±32.12)pg/mL、2.48±0.33,均高于对照组患者[(326.26±28.12)pg/mL、1.03±0.24],PON-1水平为(152.23±8.78)pg/mL,低于对照组患者[(212.26±8.23)pg/mL],差异均有统计学意义(P<0.05)。重度组患者的ROS水平和miR-20a相对表达水平分别为(498.22±18.45)pg/mL、2.64±0.37,均高于轻度组患者[(433.28±23.16)pg/mL、1.97±0.21],PON-1水平为(142.04±5.99)pg/mL,低于轻度组患者[(164.56±5.48)pg/mL],差异均有统计学意义(P<0.05)。重度组患者的血清IL-6、IL-8、MCP-1、TNF-α水平分别为(33.26±2.45)、(29.55±3.16)、(39.45±2.78)、(29.88±2.64)pg/mL,均高于轻度组患者[(17.22±2.23)、(23.66±3.15)、(32.45±2.54)、(21.02±2.37)pg/mL],差异均有统计学意义(P<0.05)。ROS水平,miR-20a相对表达水平,IL-6、IL-8、MCP-1、TNF-α水平与患者的临床分期呈正相关(r=0.847、0.751、0.862、0.685、0.830、0.859,P<0.05),PON-1水平与患者的临床分期呈负相关(r=-0.856,P<0.05)。ROS、PON-1水平,miR-20a相对表达水平诊断重度卵巢型子宫内膜异位的ROC曲线的曲线下面积(AUC)分别为0.991、0.997、0.936,具有较好诊断价值(P<0.05)。结论ROS水平、miR-20a相对表达水平在卵巢型EMs患者中升高,PON-1水平表达下调,且对其病情严重程度具有一定诊断价值,可用于临床辅助诊断。

Oxygen radical formation does not have an impact in the treatment of severe acute experimental pancreatitis using free cellular hemoglobin

瞄准：Microcirculatory 机能障碍和免费的氧激进分子是在严重尖锐胰腺炎的致病的重要因素。另外的氧交货可能每氧化提高类脂化合物，但是可以也改进胰腺的微循环。这研究在严重尖锐胰腺炎的一个啮齿类动物模型在氧激进分子和微循环的形成上估计免费细胞的牛的血红素的效果。方法：在尖锐胰腺炎 Wistar 老鼠的正式就职以后的十五分钟收到了任何一个 0.8 mL 牛的血红素(HBOC-200 ) ， hydroxyethyl 淀粉(HES ) 或保证血量正常替换的生理盐水的 2.4 mL。在检查的 6 h 以后，胰每氧化产品 malondialdehyde (MDA ) 为类脂化合物的间接测量被切除并且很快处理了并且在胰腺的织物减少了谷胱甘肽(GSH ) 。结果：HBOC-200 的单个申请改进了胰腺的微循环并且显著地减少了组织病理学说的织物损坏。MDA 的织物集中没在这些组之间不同。另外，在 GSH 层次的差别都没被检测。结论：不过 HBOC-200 和 HES 的单个申请改进胰腺的微循环，在类脂化合物的没有差别每氧化，产品被检测。另外的氧供应(HBOC-200 ) 的有益的效果不每氧化导致提高的类脂化合物。

Election Spin Resonance Studies of Free Radical Formation and Oxygen Consumption of Lens Epithelium During Ultraviolet Exposure

A long life election spin resonance (ESR) signal at g=2.0006 was observed in the normal lens epithelium and cortical fibers. During ultraviolet (UV) exposure, a new ESR signal at g = 2.0060 was found in the lens epithelium. But this specific signal was not detected in the lens cortical fibers. This suggested that lens epithelial cells were more susceptible to the free radical formation which was induced by UV light. By means of ESR spin probe oxymetry, the oxygen uptake of lens epithelial cells was meas...

THE ACTION OF CAPTOPRIL ON SCAVENGING OXYGEN FREE RADICALS

The protective effect of captopril on ischemic myocardium was studied in 40 patients with congenital heart disease accompanied with pulmonary hypertention.Twenty of these patients received captopril 50 mg b.i.d.for three months preoperatively (Group A).The other 20 patients without pretreatment with captopril were used as controls (Group B).In Group A,the cardiac output increased and the pulmonary pressure decreased significantly in the first 24 hours postoperatively,indicating a high cardiac output with a low vascular resistance.The differences or CPK,CPKMB,LDH release between Group A and Group B were highly significant or siguificant from the end of operation to 24 hours postoperatively. Myocardial protection in the reduction of the release of CPK, CPK-MB, LDH might be attributed to action on scavenging oxygen free radicals.

Direct Measurement of Oxygen Free Radicals of the Liver Tissue Following Shock/Reperfusion and Its Significance

ＤｉｒｅｃｔＭｅａｓｕｒｅｍｅｎｔｏｆＯｘｙｇｅｎＦｒｅｅＲａｄｉｃａｌｓｏｆｔｈｅＬｉｖｅｒＴｉｓｓｕｅＦｏｌｌｏｗｉｎｇＳｈｏｃｋ／ＲｅｐｅｒｆｕｓｉｏｎａｎｄＩｔｓＳｉｇｎｉｆｉｃａｎｃｅＺｈａｏＺｈｉｑｕａｎ（赵志泉）ＸｕＳｈｕｎｆｕ（徐顺福...

The influence of ultraviolet blood irradiation and oxygenation on oxygen free radicals metabolism in rabbits with soman intoxication

Objective: To investigate the effect of ultraviolet blood irradiation and oxygenation (UBIO) on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods: One hundred rabbits were randomly divided into 5 groups: normal control group, intoxication group, routine therapy group. UBIO therapy group and combined therapy group. After 14 d, the concentration of malondiadehyde(MDA) and activity of superoxide dismutase(SOD), glutathionperoxidase(GSH-Px), catalase (CAT) and total antioxidative capacity (T-AOC) in serum were determined respectively. Results: Compared with the normal control group, the concentration of MDA and activity of CAT in the intoxication group were significantly higher (P < 0. 05). but SOD. GSH-Px activity and T-AOC were significantly lower (P<0. 05). After UBIO or combined therapy, serum MDA level was significantly lower in comparison with intoxication group (P<0. 05). but the activity of SOD. GSH-Px, CAT and T-AOC were higher than intoxication group(P<0. 05). Conclusion: There is an obvious oxygen free radical injury in rabbits with a-cute soman intoxication. UBIO can improve the antioxidation ability of rabbits and may be applied to treat acute soman intoxication as adjunctive therapy.

EFFECT OF ACUPUNCTURE ON BLOOD OXYGEN FREE RADICAL AND NO LEVELS IN TREATMENT OF APOPLECTIC SEQUELAE

Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subjected into this study and randomly divided into "JIN San Zhen" group (n=30) and control group (n=31). Blood lipid peroxidase (LPO), superoxide dismutase (SOD), glutathione peroxidase(GSH Px) and nitric oxide (NO) contents before and after acupuncture treatment were determined with radioimmunoassay. In both groups, acupuncture was given once daily, six times a week, with 4 weeks being a therapeutic course and with the interval between two weeks being a week, 3 courses all together. In "JIN San Zhen" group, acupoints of "JIN San Zhen" were used predominately, while in control group, scalp point Motor Sensory Area (MS 8) was used as the main point. Results: Self comparison showed that after 3 courses of treatment, in both groups, LPO and NO levels decreased significantly (P<0.05-0.01), SOD and GST Px values increased considerably (P<0.05-0.01). Comparison between two groups indicated that the effects of "JIN San Zhen" group are significantly superior to those of control group in raising blood SOD and GST Px levels (P<0.05-0.01) and in lowering blood NO content (P<0.01). Analysis on the correlation between the restoration of neural function and the changes of LPO, SOD and GST Px levels suggested that the effect of acupuncture in improving neural function may be related to changes of the aforementioned indexes. Conclusion: Acupuncture therapy can significantly lower blood LPO and NO levels and evidently raise blood SOD and GST Px levels in stroke patients.

Effect of dexmedetomidine combined with ulinastatin on RAAS system,coagulation indexes and oxygen free radicals after acute aortic dissection surgery

Objective: To study the effect of dexmedetomidine combined with ulinastatin on RAAS system, coagulation indexes and oxygen free radicals after acute aortic dissection surgery. Methods: A total of 48 patients with aortic dissection who accepted endovascular graft exclusion treatment in the hospital between May 2014 and February 2017 were selected and randomly divided into two groups, group A received dexmedetomidine, ulinastatin combined with general anesthesia, and group B received general anesthesia. The RAAS hormones, coagulation indexes and oxygen free radical indexes were measured 1 d before surgery, during operation and 24 h after surgery. Results: During operation and after operation, serum REN, AT-II, ALD, FIB, DD, MDA and MPO contents as well as APTT and TT levels of both groups of patients were significantly higher than those before operation while TAOC and TSOD contents were significantly lower than those before operation, and serum REN, AT-II, ALD, FIB, DD, MDA and MPO contents as well as APTT and TT levels of group A were significantly lower than those of group B while TAOC and TSOD contents were significantly higher than those of group B. Conclusion: Dexmedetomidine combined with ulinastatin for acute aortic dissection can inhibit RAAS system and oxygen free radical generation and improve coagulation function.

Effect of early rehabilitation training on oxygen free radical generation and nerve injury in patients with cerebral hemorrhage

Objective: To study the effect of early rehabilitation training combined with edaravone on oxygen free radical generation and nerve injury in patients with cerebral hemorrhage. Methods: A total of 56 patients with acute cerebral hemorrhage who were treated in Zigong Third People's Hospital between July 2014 and March 2017 were selected and randomly divided into early rehabilitation group and routine rehabilitation group, the early rehabilitation group began the rehabilitation training 2 d after cerebral hemorrhage condition was stabilized, and routine rehabilitation group began the rehabilitation training 14 d after cerebral hemorrhage. Serum contents of oxygen free radicals, nerve injury markers and neurotrophic molecules were detected 28 d and 56 d after cerebral hemorrhage. Results: 28 d and 56 d after cerebral hemorrhage, serum MDA, AOPP, 8-OHdG, GFAP, NSE, Tf, Ft and S100B levels of early rehabilitation group were significantly lower than those of routine rehabilitation group while BDNF, NGF, NTF-α and IGF-I levels were significantly higher than those of routine rehabilitation group. Conclusion: Early rehabilitation training combined with edaravone for cerebral hemorrhage can inhibit the oxygen free radical generation, reduce the degree of nerve injury and improve the neurotrophic state.

Do reactive oxygen species damage or protect the heart in ischemia and reperfusion?Analysis on experimental and clinical data

The role of reactive oxygen species(ROS)in ischemic and reperfusion(I/R)injury of the heart has been discussed for more than 40 years.It has been demonstrated that reperfusion triggers a multiple increase in free radical generation in the isolated heart.Antioxidants were found to have the ability to mitigate I/R injury of the heart.However,it is unclear whether their cardioprotective effect truly depends on the decrease of ROS levels in myocardial tissues.Since high doses and high concentrations of antioxidants were experimentally used,it is highly likely that the cardioprotective effect of antioxidants depends on their interaction not only with free radicals but also with other molecules.It has been demonstrated that the antioxidant N-2-mercaptopropionyl glycine or NDPH oxidase knockout abolished the cardioprotective effect of ischemic preconditioning.Consequently,there is evidence that ROS protect the heart against the I/R injury.

Middle Molecular Weight Heparinyl Amino Acid Derivatives (MHADs) Function as Indirect Radical Scavengers in Vitro

We conducted the novel synthesis of middle molecular weight heparinyl amino acid derivatives (MHADs) to reduce the adverse effect of heparin (HE) based on its anticoagulant activity. Subsequently, we investigated the radical scavenging effects of 12 kinds of MHAD on cultured human umbilical vein endothelial cells (HUV-ECs) damaged by oxygen free radicals using xanthine and xanthine oxidase in vitro. As a result, middle molecular weight heparinyl phenylalanine, middle molecular weight heparinyl leucine, and middle molecular weight heparinyl tyrosine showed significant protective effects on HUV-ECs. In conclusion, these three HE derivatives might be candidates for therapeutic agents to treat diseases attributed to peroxidation.

锯缘青蟹不同器官组织中ROS与ASAFR含量的研究

采用生化测定的方法,对锯缘青蟹(Scyllaserrata)鳃、肝胰腺和肌肉等不同器官组织中活性氧(ROS)与抗超氧阴离子自由基(ASAFR)含量进行了测定。 结果表明, 不同器官组织中活性氧与抗超氧阴离子自由基含量各异。 各器官组织中活性氧含量由高到低依次为:肝胰腺>肌肉>鳃, 分别为195.04U/mg±33.46U/mg, 64.03 U/mg±12.77 U/mg和44.84U/mg±10.44U/mg。而抗超氧阴离子自由基含量则是鳃>肝胰腺>肌肉,大小分别为439.62 U/g±64.73U/g,297.89 U/g±39.75 U/g和84.24 U/g±18.03 U/g。统计分析显示,锯缘青蟹各器官组织间的活性氧与抗超氧阴离子自由基含量均存在极显著性差异(P<0.01)。这是由于不同器官组织所执行的生理功能不同造成的。

Direct detection of oxygen free radicals produced in the viscera of burned rats using electron paramagnetic resonance spectroscopy

To detect superoxide anion (O - 2 ) signals in the heart, liver, lung and kidney tissues after burns. Methods: Twenty four male rats were randomized into 4 groups. The rats of experimental groups were immersed in 100℃ water for 15 seconds and 25% third degree burn was created. Thoracotomy or laparotomy was performed at 5, 10, 15, 20 and 30 minutes after burn, and specimens of the heart, lung, liver kidney were obtained for burned rats. The specimens were then preserved in liqu id nitrogen for cryo preservation and detected immediately using EPR. Results: The signals of superoxide O radical appeared in the he art, lung, 1iver and kidney specimens 10 15 minutes after burn. Conclusions: There is a direct evidence of oxygen free radicals (OFRs) injury to viscera of burned rats between 10 15 minutes after burn.

Preparation of Elemental Tellurium Nanoparticles-Sucrose Sol and Its Antioxidant Activity in Vitro

The elemental tellurium nanoparticles （TeNPs） - sucrose sol was prepared by the reaction of sodium tellurite with ascorbic acid in sucrose aqueous solution. The results indicated that TeNPs were dispersion excellent in the TeNPs - sucrose sol and the morphology of TeNPs was needle-like with about 70 nm in width and 500 nm in length. The antioxidant activity of the TeNPs - sucrose sol in vitro was estimated by pyrogallol autoxidation method, Fenton method and oxygen radical absorbance capacity （ORAC） assay. The results showed that a certain amount of TeNPs - sucrose sol could effectively scavenge superoxide anion free radical and hydroxyl free radical with scavenging rates of 73 % and 57 %, respectively. ORAC assay was used to measure the total antioxidant capacity of TeNPs - sucrose sol. The order of ORAC values was 2.25 μmol.L-1 TeNPs - 0.025 % sucrose sol 〉 0.025 % sucrose 〉 2.25 μmol.L-1 Na2TeO3 〉 1.63 μmol.L-1 ascorbic acid. The results suggested that the TeNPs - sucrose sol had the excellent antioxidant activity and TeNPs were the dominating contributors to antioxidant capacity of the TeNPs - sucrose sol.