Objective:To explore the effect of Oxymatrine(OMT)on hepatocellular carcinoma cells and its possible action pathways.Methods:The core targets of Oxymatrine action in hepatocellular carcinoma were identified using netw...Objective:To explore the effect of Oxymatrine(OMT)on hepatocellular carcinoma cells and its possible action pathways.Methods:The core targets of Oxymatrine action in hepatocellular carcinoma were identified using network pharmacology and validated using in vitro experiments.CCK8 assay and scratch assay were used to detect the effects of Oxymatrine on the proliferation and migration of MHCC97H(TP53 mutant)cells,and Western blot assay and qPcr assay were used to find out the expression of the core targets after Oxymatrine acted on MHCC97H cells at the protein level and mRNA level,respectively.Results:Network pharmacology showed that the core target was TP53 protein;CCK8 assay showed that the IC50 at 24 h and 48 h was 9.64 mg/ml and 7.47 mg/ml,respectively;Scratch assay proved that Oxymatrine markedly inhibited the migratory ability of MHCC97H cells;Western blot assay and qPcr assay found that TP53 gene expression was down-regulated.The western blot experiment and qPcr experiment found that TP53 gene expression was down-regulated.Conclusions:Oxymatrine significantly inhibited the proliferation and migration of hepatocellular carcinoma MHCC97H cells,and its mechanism of action may be related to TP53.展开更多
Aim The oxymatrine phospholipid complexs were prepared, and its activity against hepatitis B virus in vitro were studied. Methods Using tetrahydrofuran as a reaction medium, oxymatrine and phospholipids were resolved ...Aim The oxymatrine phospholipid complexs were prepared, and its activity against hepatitis B virus in vitro were studied. Methods Using tetrahydrofuran as a reaction medium, oxymatrine and phospholipids were resolved into the medium, Oxymatrine phospholipid eomplexs were prepared. The toxicity measurements and inhibitory effect on HBsAg, HBeAg, and HBVDNA of oxymatrine phospholipid complex in 2.2.15 cells were studied respectively. Results The content of oxymatrine in the phospholipids eomplexs prepared was 24,86% （W/W）. The TCO of the oxymatrine phospholipid eomplexs was 250 μmol·L^- 1 The inhibitory effect of HBsAg, HBeAg, HBV-DNA of 2.2.15 cells treated by oxymatrine phospholipid complex were higher than those of the oxymatrine. Conclusion Oxymatrine phospholipid complex can have stronger effective activity against hepatitis B virus compared with oxymatrine. So oxymatrine phospholipid eomplexs were showing its potential antiviral activity in hepatitis B treatment.展开更多
探讨氧化苦参碱(OMT)乳膏对小鼠皮肤屏障功能的修复作用。将70只雄性ICR小鼠随机分为7组,除正常组小鼠外,采用胶带粘贴法模拟机械损伤,复制皮肤屏障功能障碍小鼠模型。造模后经皮连续给药5天,拍摄照片并测定造模区域的经表皮失水量(TEWL...探讨氧化苦参碱(OMT)乳膏对小鼠皮肤屏障功能的修复作用。将70只雄性ICR小鼠随机分为7组,除正常组小鼠外,采用胶带粘贴法模拟机械损伤,复制皮肤屏障功能障碍小鼠模型。造模后经皮连续给药5天,拍摄照片并测定造模区域的经表皮失水量(TEWL)。实验结束后,HE染色观察皮肤组织形态学变化,酶联免疫吸附法(ELISA)检测皮肤组织中肿瘤坏死因子α(TNF-α)、白细胞介素-8(IL-8)的含量,通过实时定量PCR(RT-qPCR)检测紧密连接蛋白(ZO-1)、丝聚蛋白(FLG)、水通道蛋白3(AQP3)mRNA的表达量,通过Western blot检测屏障相关蛋白如封闭蛋白(OCC)、FLG、紧密连接蛋白(Claudin)的表达量。结果显示,与模型组相比,OMT各剂量组均能降低皮肤TEWL,减轻炎症浸润,改善皮肤病理状态,同时高剂组皮肤组织中TNF-α等炎症因子的含量显著降低(P<0.05),ZO-1、FLG m RNA及OCC、FLG、Claudin蛋白的表达显著升高(P<0.05)。因此,OMT具有修复皮肤屏障功能的作用。展开更多
文摘Objective:To explore the effect of Oxymatrine(OMT)on hepatocellular carcinoma cells and its possible action pathways.Methods:The core targets of Oxymatrine action in hepatocellular carcinoma were identified using network pharmacology and validated using in vitro experiments.CCK8 assay and scratch assay were used to detect the effects of Oxymatrine on the proliferation and migration of MHCC97H(TP53 mutant)cells,and Western blot assay and qPcr assay were used to find out the expression of the core targets after Oxymatrine acted on MHCC97H cells at the protein level and mRNA level,respectively.Results:Network pharmacology showed that the core target was TP53 protein;CCK8 assay showed that the IC50 at 24 h and 48 h was 9.64 mg/ml and 7.47 mg/ml,respectively;Scratch assay proved that Oxymatrine markedly inhibited the migratory ability of MHCC97H cells;Western blot assay and qPcr assay found that TP53 gene expression was down-regulated.The western blot experiment and qPcr experiment found that TP53 gene expression was down-regulated.Conclusions:Oxymatrine significantly inhibited the proliferation and migration of hepatocellular carcinoma MHCC97H cells,and its mechanism of action may be related to TP53.
文摘Aim The oxymatrine phospholipid complexs were prepared, and its activity against hepatitis B virus in vitro were studied. Methods Using tetrahydrofuran as a reaction medium, oxymatrine and phospholipids were resolved into the medium, Oxymatrine phospholipid eomplexs were prepared. The toxicity measurements and inhibitory effect on HBsAg, HBeAg, and HBVDNA of oxymatrine phospholipid complex in 2.2.15 cells were studied respectively. Results The content of oxymatrine in the phospholipids eomplexs prepared was 24,86% （W/W）. The TCO of the oxymatrine phospholipid eomplexs was 250 μmol·L^- 1 The inhibitory effect of HBsAg, HBeAg, HBV-DNA of 2.2.15 cells treated by oxymatrine phospholipid complex were higher than those of the oxymatrine. Conclusion Oxymatrine phospholipid complex can have stronger effective activity against hepatitis B virus compared with oxymatrine. So oxymatrine phospholipid eomplexs were showing its potential antiviral activity in hepatitis B treatment.
文摘探讨氧化苦参碱(OMT)乳膏对小鼠皮肤屏障功能的修复作用。将70只雄性ICR小鼠随机分为7组,除正常组小鼠外,采用胶带粘贴法模拟机械损伤,复制皮肤屏障功能障碍小鼠模型。造模后经皮连续给药5天,拍摄照片并测定造模区域的经表皮失水量(TEWL)。实验结束后,HE染色观察皮肤组织形态学变化,酶联免疫吸附法(ELISA)检测皮肤组织中肿瘤坏死因子α(TNF-α)、白细胞介素-8(IL-8)的含量,通过实时定量PCR(RT-qPCR)检测紧密连接蛋白(ZO-1)、丝聚蛋白(FLG)、水通道蛋白3(AQP3)mRNA的表达量,通过Western blot检测屏障相关蛋白如封闭蛋白(OCC)、FLG、紧密连接蛋白(Claudin)的表达量。结果显示,与模型组相比,OMT各剂量组均能降低皮肤TEWL,减轻炎症浸润,改善皮肤病理状态,同时高剂组皮肤组织中TNF-α等炎症因子的含量显著降低(P<0.05),ZO-1、FLG m RNA及OCC、FLG、Claudin蛋白的表达显著升高(P<0.05)。因此,OMT具有修复皮肤屏障功能的作用。
