Abstract ：Aim To assess therapeutic effect and mechanism of Oxytropis falcata Bung gel compound （OFBGC） in rats. Methods Wistar rats were divided into： model group, vehicle group, administration groups（low-dose, ...Abstract ：Aim To assess therapeutic effect and mechanism of Oxytropis falcata Bung gel compound （OFBGC） in rats. Methods Wistar rats were divided into： model group, vehicle group, administration groups（low-dose, middle- dose and high-dose group）, positive control and normal control. Deep Ⅱ° burn rat model was used and the animals were treated （2/d）, respectively. In order to assess the wound healing effect, HE staining was conducted. Immuno- histochemical detection for EGF, VEGF and CD34 were performed. ELISA detection for EGF, VEGF, p38 and IL-1β were carried out in serum. Results According to HE staining, administration groups could reduce conspicuously wound scabby time, accelerate wound healing, promote the hyperplasia of sebaceous glands and hair follicles, de- crease the inflammatory cells and relieve swelling in different degree. The expression of EGF and CD34 in burning tissue increased remarkably in 7th day from immunohistochemical assessment. Serum EGF expression went up con- spicuously （P 〈 0. 01 ）, but p38 and IL-1β levels were dramatically lower compared to the model groups. Conclusions OFBGC could promote the wound healing in deep Ⅱ° burn rats. The mechanism may be related, at least in part, to increasing the biosynthesis and release of EGF and CD34 in burning tissue, decreasing serum p38 and IL-1β levels.展开更多
文摘Abstract ：Aim To assess therapeutic effect and mechanism of Oxytropis falcata Bung gel compound （OFBGC） in rats. Methods Wistar rats were divided into： model group, vehicle group, administration groups（low-dose, middle- dose and high-dose group）, positive control and normal control. Deep Ⅱ° burn rat model was used and the animals were treated （2/d）, respectively. In order to assess the wound healing effect, HE staining was conducted. Immuno- histochemical detection for EGF, VEGF and CD34 were performed. ELISA detection for EGF, VEGF, p38 and IL-1β were carried out in serum. Results According to HE staining, administration groups could reduce conspicuously wound scabby time, accelerate wound healing, promote the hyperplasia of sebaceous glands and hair follicles, de- crease the inflammatory cells and relieve swelling in different degree. The expression of EGF and CD34 in burning tissue increased remarkably in 7th day from immunohistochemical assessment. Serum EGF expression went up con- spicuously （P 〈 0. 01 ）, but p38 and IL-1β levels were dramatically lower compared to the model groups. Conclusions OFBGC could promote the wound healing in deep Ⅱ° burn rats. The mechanism may be related, at least in part, to increasing the biosynthesis and release of EGF and CD34 in burning tissue, decreasing serum p38 and IL-1β levels.
