Exercise-induced modulation of miR-149-5p and MMP9 in LPS-triggered diabetic myoblast ER stress: licorice glycoside E as a potential therapeutic target

Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.

An Analysis of Development as A Reflective Teacher in Higher Education

Effective teaching is every teacher's teaching goal.How to reach the aim is to reflect on the teaching.The following document describes the development on how to reflect the teaching.The main purpose of this analysis is to show how effective and important the reflection on the teaching and to show how useful the reflection which can make the teacher change the way of teaching,encouraging the students to adopt a deep approach in their learning.

MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis

BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of GC.METHODS Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and cells.The ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,respectively.Cell proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow cytometry.Expression of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western blot.Targets of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter system.Serpin family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical staining.The correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot analysis.The association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and miR-145-5p.The mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude mice.RESULTS GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this miRNA.Overexpression of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 expression.Kaplan-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 expression.Nude mouse tumorigenesis experiments confirmed that miR-145-5p targets SERPINE1 to regulate extracellular signal-regulated kinase-1/2(ERK1/2).CONCLUSION This study found that miR-145-5p inhibits tumor progression and is expressed in lower amounts in patients with GC.MiR-145-5p was found to affect GC cell proliferation,migration,and invasion by negatively regulating SERPINE1 levels and controlling the ERK1/2 pathway.

Analysis of the effect of professional teaching staff construction in the training of low-level nurses in operating room

Objective:To explore the application effect of constructing professional teaching staff in low-level training in operating room,so as to further optimize the teaching strength of operating room in our hospital and improve the training effect of junior nurses.Methods:Thirty-eight low-level nurses in the operating room of a third-class hospital in Yantai were selected for half a year's nurse training.With theoretical scores,overall nursing performance,nurses'self-awareness evaluation system and nurses'satisfaction with tutors as evaluation criteria,and based on the selection of high-quality teachers,various evaluation indexes before and after the training of low-level nurses in the operating room were compared and evaluated through the cultivation of practical teaching teachers'ability and the application of a series of teaching methods based on the change of c ompetence-based education(CBE)teaching mode,the application of guided learning interactive canadian education(BOPPPS)teaching model and p roblem-b ased l earning(PBL)teaching method.Results:After the training,the examination scores of low-level nurses were significantly improved(P<0.05),the teaching quality was highly recognized by low-level nurses,the quality of low-level nurses was improved,and patients'satisfaction with nurses was improved.Conclusion:It is of great significance to assist the construction of professional teachers in evidence-based medicine.Through the training of practical teaching teachers'ability and the application of a series of teaching methods based on the change of CBE teaching model,the application of BOPPPS teaching model and PBL teaching method,the training results of nurses have been significantly improved and improved,which is worthy of clinical reference and promotion.

HPV E6/E7mRNA检测与P16/Ki-67免疫组化检测对宫颈CIN2级病变的筛查价值探究

目的:研究人乳头瘤病毒(Human papilloma virus,HPV)E6/E7mRNA与免疫组化抑癌基因P16/细胞增殖核抗原Ki-67在宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)2级病变筛查中的应用价值。方法:选取2020年2月至2023年2月期间本院收治的92例宫颈炎症及病变患者作为研究对象。根据病理检查结果,将CIN2级患者纳入研究组(n=45),宫颈CIN1级和宫颈炎患者纳入对照组(n=47)。对比两组HPV E6/E7mRNA、P16/Ki-67单独及联合检测阳性表达率差异,采用Logistic回归分析影响CIN2诊断的危险因素,并采用受试者工作曲线分析HPV E6/E7mRNA与P16/Ki-67单独及联合检测在宫颈CIN2级病变筛查的价值。结果:研究组HPV E6/E7mRNA、P16/Ki-67单独检测阳性率、联合检测阳性率(82.22%,77.78%,84.44%)高于对照组(63.83%,48.94%,53.19%)(P<0.05)。Logistic回归分析显示HPV E6/E7mRNA、P16/Ki-67是影响CIN2诊断的危险因素(P<0.05);HPV E6/E7mRNA、P16/Ki-67联合检测CIN2级病变的受试者工作特征曲线下面积为0.688(95%CI:0.579~0.798),优于HPV E6/E7mRNA、P16/Ki-67单独预测[0.592(95%CI:0.476~0.708);0.634(95%CI:0.519~0.748)]。联合检测的准确度、灵敏度、特异度(68.8%,84.4%,53.2%)均高于单独检测HPV E6/E7mRNA(59.2%,82.2%,36.2%)、P16/Ki-67(63.4%,77.8%,48.9%)。结论:HPV E6/E7mRNA与P16/Ki-67免疫组化检测在宫颈CIN2级病变筛查中具有一定的应用价值,可以显著提高筛查宫颈CIN2级病变的诊断效能。

Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma

AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,Egypt.Fifteen wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal controls.Laboratory investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and p27.Cyclin D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 immuno-reactivity.The expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied cases.Correlation analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC plays a vital role in the development and differentiation of HCC;while,Cyclin E may be a useful marker for monitoring tumor behavior.p21 and p27 can be used as predictive markers for HCC.Furthermore,higher expression of Rb/p105 as well as inverse relation with p21 and histologic grades suggests its important role in hepatic carcinogenesis.

High Energy Physics and Cosmology as Computation

The present paper is basically written as a non-apologetic strong defence of the thesis that computation is part and parcel of a physical theory and by no means a mere numerical evaluation of the prediction of a theory which comes towards the end. Various general considerations as well as specific examples are given to illustrate and support our arguments. These examples range from the practical aspect to almost esoteric considerations but at the end, everything converges towards a unity of theory and computation presented in the form of modern fractal logic and transfinite quantum field theory in a Cantorian spacetime. It is true that all our examples are taken from physics but our discussion is applicable in equal measure to a much wider aspect of life.

A genetic variant in pseudogene E2F3P1 contributes to prognosis of hepatocellular carcinoma

Certain pseudogenes may regulate their protein-coding cousins by competing for miRNAs and play an active biological role in cancer. However, few studies have focused on the association of genetic variations in pseudogenes with cancer prognosis. We selected six potentially functional single nucleotide polymorphisms （SNPs） in cancerrelated pseudogenes, and performed a case-only study to assess the association between those SNPs and the prognosis of hepatocellular carcinoma （HCC） in 331 HBV-positive HCC patients without surgical treatment. Log-rank test and Cox proportional hazard models were used for survival analysis. We found that the A allele of rs9909601 in E2F3P1 was significantly associated with a better prognosis compared with the G allele [adjusted hazard ratio （HR） = 0.69, 95% confidence interval （CI） = 0.56-0.86, P = 0.001]. Additionally, this protective effect was more predominant for patients without chemotherapy and transcatheter hepatic arterial chemoembolization （TACE） treatment. Interestingly, we also detected a statistically significant multiplicative interaction between genotypes of rs9909601 and chemotherapy or TACE status on HCC survival （P for multiplicative interaction 〈 0.001）. These findings indicate that rs9909601 in the pseudogene E2F3P1 may be a genetic marker for HCC prognosis in Chinese.

TGF-β2-induced NEAT1 regulates lens epithelial cell proliferation,migration and EMT by the miR-26a-5p/FANCE axis

AIM:To explore the regulatory mechanism of nuclear paraspeckle assembly transcript 1(NEAT1)in the pathogenesis of posterior capsule opacification(PCO).METHODS:Quantitative reverse transcription polymerase chain reaction(RT-q PCR)was executed to analyze NEAT1 and micro RNA(miR)-26a-5p expression in transforming growth factor-beta 2(TGF-β2)-disposed lens epithelial cells(LECs).The proliferation,cell cycle progression,apoptosis,and migration of TGF-β2-disposed LECs were evaluated.The relationship between NEAT1 or fanconi anemia(FA)complementation group E(FANCE)and miR-26a-5p was verified by dual-luciferase reporter assay.RESULTS:TGF-β2 induced NEAT1 expression in LECs.NEAT1 inhibition accelerated apoptosis,cell cycle arrest,decreased proliferation,epithelial-mesenchymal transition(EMT),and migration of TGF-β2-disposed LECs.NEAT1 sponged miR-26a-5p to further regulate FANCE expression.Rescue experiments presented that miR-26a-5p downregulation overturned NEAT1 silencing-mediated impacts on TGF-β2-disposed LEC biological behaviors.Additionally,FANCE overexpression reversed miR-26a-5p mimic-mediated impacts on TGF-β2-disposed LEC biological behaviors.CONCLUSION:TGF-β2-induced NEAT1 facilitates LEC proliferation,migration,and EMT by upregulating FANCE via sequestering miR-26a-5p.

cyclin E、p53、CD44s、CD44v6与早期乳腺癌的相关性及预后预测分析

目的分析肿瘤组织的细胞周期素E(cyclin E)和p53、CD44s、CD44v6蛋白与早期乳腺癌的相关性及预后预测价值。方法选取2018年7月—2020年7月喀什地区第二人民医院收治的80例临床分期为Ⅰ~Ⅱ期的早期乳腺癌患者进行回顾性分析,将其分为恶性组;另选取同期喀什地区第二人民医院收治的80例通过病理学检验确诊为良性乳腺肿瘤患者,将其分为良性组。分别对两组患者留存的肿瘤组织进行免疫组化检测,检测cyclin E、p53、CD44s、CD44v6表达,并对比上述指标的阳性率,应用Spearman相关分析方法分析cyclin E、p53、CD44s、CD44v6与早期乳腺癌的相关性。所有患者均采取手术治疗,随后对80例早期乳腺癌患者进行3年随访,依照随访结果将患者分为2个亚组,即预后不良组(n=25)和预后良好组(n=55),对比两组患者一般临床特征及cyclin E、p53、CD44s、CD44v6阳性率,并应用Logistic回归分析cyclin E、p53、CD44s、CD44v6对早期乳腺癌预后的预测价值。结果恶性组患者cyclin E、p53、CD44s、CD44v6阳性率分别为42.50%、61.25%、77.50%、81.25%,明显高于良性组1.25%、5.00%、10.00%、12.50%(P<0.05);Spearman相关分析结果显示:cyclin E、p53、CD44s、CD44v6与早期乳腺癌呈正相关(P<0.05);预后良好组与预后不良组患者绝经情况、年龄、病理类型、治疗方式、三阴性乳腺癌、肿瘤大小、组织分化程度对比,差异无统计学意义(P>0.05),预后良好组与预后不良组患者临床分期、cyclin E、p53、CD44s、CD44v6阳性例数对比,差异有统计学意义(P<0.05);Logistic回归分析结果表明:cyclin E、p53、CD44s、CD44v6阳性表达为影响早期乳腺癌预后的独立影响因素(P<0.05)。结论cyclin E、p53、CD44s、CD44v6表达水平与乳腺癌的发生、发展具有明显关系,且上述指标呈阳性表达为早期乳腺癌预后不良的独立预测因素。

Ck34β E12、P63、P504s免疫组织化学染色在前列腺癌诊断中的应用

目的探讨Ck34βE12、P63、P504s在前列腺癌中的表达及前列腺良恶性病变中的鉴别诊断意义。方法应用免疫组化S2P法对11例前列腺癌,315例前列腺结节性增生,25例前列腺低级别病例和14例前列腺高级别病例进行Ck34βE12、P63、P504s的表达。结果 Ck34βE12、P63和P504s在11例前列腺癌中的阳性率分别为：P63为100%阴性表达;Ck34βE12和P63在315例中前列腺结节性增生、14例伴部份腺上皮PIN高级别病变和25例前列腺部分腺上皮低级别PIN病变中存在着不同的差异表达。结论联合检测Ck34βE12、P63、P504s对前列腺前腺良恶性病变有重要的鉴别诊断学意义。

p16^(INK4A)和HPV16 E7/HPV18 E6在ASCUS中的表达及临床意义

目的:探讨p16 INK4A和HPV16 E7/HPV18 E6在宫颈细胞学诊断为非典型性鳞状细胞不明确意义型(ASCUS)中的表达及筛查潜在宫颈病变的价值。方法:对150例ASCUS患者行阴道镜检查并取活检,同时对该150例患者的TCT标本进行免疫细胞化学染色检测p16INK4A的表达和RT-PCR法检测其中HPV16型E7蛋白和18型E6蛋白(HPV16 E7/HPV18 E6)mRNA的表达。结果:p16INK4A和HPV16 E7/HPV18 E6 mRNA在ASCUS中表达的阳性率分别为37.33%和46.67%,随着病理级别的增加,p16INK4A和HPV16E7/HPV18 E6 mRNA表达的阳性率也随之增加;p16INK4A和HPV16 E7/HPV18 E6 mRNA筛查ASCUS中宫颈病变的灵敏度、特异度、阳性预测值和阴性预测值分别为0.88、0.95、0.91、0.93和0.81、0.75、0.67、0.86,在p16INK4A和HPV16 E7/HPV18 E6 mRNA阳性的样本中,宫颈病变发生率分别为91.07%和67.14%,均明显高于阴性样本中的发病率7.45%和13.75%(P<0.001);ASCUS中宫颈病变样本中p16INK4A和HPV16 E7/HPV18 E6 mRNA呈高表达,且具有较高的一致性(κ=0.6475)。结论:p16INK4A和HPV16 E7/HPV18 E6 mRNA在ASCUS中病理诊断为宫颈病变的样本中均呈高表达,对筛查潜在的宫颈病变具有重要意义,其中p16INK4A的筛查效能优于HPV16E7/HPV18E6mRNA;p16INK4A能间接反映HPV的转录活性,在ASCUS的分流中有重要意义,且可视性的p16INK4A免疫染色比HPV检测更直观。

CHARACTERISTICS OF STRUCTURE OF IMPACT COPOLYMERS OF POLYPROPYLENE WITH LOW ETHYLENE CONTENTS

In the present work, the structure and impact properties of copolymers of polypropylenewith low ethylene contents have been investigated. Based on the results of ^(13)C--NMR, FTIR,WAXD, DSC, PLM and SEM, the relationship between impact properties and morphology of thecopolymers has been discussed. The high impact properties of copolymer ICP2 may attribute tothe relatively higher ethylene content and homogeneous ethylene unit distribution. The size andits distribution of spherulite in the copolymers and cycloid cavities dispersed in polypropylenecontinue phase may also be two important factors which affect the impact properties of these materials.

The Prognostic Value of Pathological and Molecular Margins Marked by p53 and eIF4E in Laryngeal Carcinoma

Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was studied in 253 cases and 67 cases respectively, the latter were pathological negative margin chosen from the former. Immunohistochemisty was used to detect the expression of eIF4E and p53 proteins. Results: The rate of pathological, p53 and eIF4E positive margins was 20.2%, 19.4% and 32.8% respectively. The recurrent rate of those with positive margins was higher than that of negative margins, which including pathological margin (70.6% vs 35.1%, P =0.0000), p53 margin (69.2% vs 33.3%, P =0.018) and eIF4E margin (63.6% vs 28.9%, P =0.018); The survival rate of those with negative margins was higher than those with positive margins, including pathological margin (the 5-year cumulative survival rate was 37.52% and 64.37% respectively, P =0.0023), p53 margin (the 5-year cumulative survival rate was 24.62% and 75.69% respectively, P =0.0012) and eIF4E margin (the 5-year cumulative survival rate was 43.31% and 77.52% respectively, P =0.0006). Conclusion: The prognosis of those with both pathological and molecular positive margins was worse than that of the negative margins; Both the eIF4E and p53 were useful markers to pick out the poor prognostic patients from those with pathological negative margin, and the former seemed to be more potential.

Dietary lipid and gross energy affect protein utilization in the rare minnow Gobiocypris rarus

An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutrition standardization for model fi sh. Twenty-four diets were formulated to contain three gross energy(10, 12.5, 15 kJ/g), four protein(20%, 25%, 30%, 35%), and two lipid levels(3%, 6%). The results showed that optimal dietary E/P was 41.7–50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate(SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ /g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing eff ect. Optimal protein decreased from 35% to 25%–30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%–35% and 10–12.5 k J/g, respectively.

Impact of global sea surface temperature on the recent early winter Arctic tropospheric warming in coordinated large ensemble simulations

Coordinated numerical ensemble experiments with six different state-of-the-art atmosphere models were used to evaluate and quantify the impact of global SST(from reanalysis data)on the early winter Arctic warming during 1982-2014.Two sets of experiments were designed:in the first set(EXP1),OISSTv2 daily sea-ice concentration and SST variations were used as the lower boundary forcing,while in the second set(EXP2)the SST data were replaced by the daily SST climatology.In the results,the multi-model ensemble mean of EXP1 showed a nearsurface(~850 hPa)warming trend of 0.4℃/10 yr,which was 80%of the warming trend in the reanalysis.The simulated warming trend was robust across the six models,with a magnitude of 0.36-0.50℃/10 yr.The global SST could explain most of the simulated warming trend in EXP1 in the mid and low troposphere over the Arctic,and accounted for 58%of the simulated near-surface warming.The results also suggest that the uppertropospheric warming(~200 hPa)over the Arctic in the reanalysis is likely not a forced signal;rather,it is caused by natural climate variability.The source regions that can potentially impact the early winter Arctic warming are explored and the limitations of the study are discussed.

Epidermal growth factor upregulates Skp2/Cks1 and p27^(kip1) in human extrahepatic cholangiocarcinoma cells

AIM:To evaluate the expression status of S-phase kinase-associated protein 2(Skp2)/cyclin-dependent kinases regulatory subunit 1(Cks1)and p27kip1,and assess the prognostic significance of Skp2/Cks1 expression with p27kip1in patients with extrahepatic cholangiocarcinoma.METHODS:Seventy-six patients who underwent curative resection for histologically confirmed extrahepatic cholangiocarcinoma at our institution from December1994 to March 2008 were enrolled.Immunohistochemical staining for Skp2,Cks1,p27kip1,and Ki67,along with other relevant molecular biologic experiments,were performed.RESULTS:By Cox regression analyses,advanced age(>65 years),advanced AJCC tumor stage,poorly differentiated histology,and higher immunostaining intensity of Skp2 were identified as independent prognostic factors in patients with extrahepatic cholangiocarcinoma.Exogenous epidermal growth factor(EGF,especially 0.1-10 ng/mL)significantly increased the proliferation indices by MTT assay and the mRNA levels of Skp2/Cks1 and p27kip1in SNU-1196,SNU-1079,and SNU-245 cells.The protein levels of Skp2/Cks1(from nuclear lysates)and p27kip1(from cytosolic lysate)were also significantly increased in these cells.There were significant reductions in the protein levels of Skp2/Cks1and p27kip1(from nuclear lysate)after the treatment of LY294002.By chromatin immunoprecipitation assay,we found that E2F1 transcription factor directly binds to the promoter site of Skp2.CONCLUSION:Higher immunostaining intensity of Skp2/Cks1 was an independent prognostic factor for patients with extrahepatic cholangiocarcinoma.EGF upregulates the mRNA and protein levels of Skp2/Cks1and p27kip1via the PI3K/Akt pathway and direct binding of E2F1 transcription factor with the Skp2 promoter.

Development Characteristics of Private P.E Economy

The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.

p16/Ki67双染、HPV E6/E7 mRNA检测对于TCT检查为低级别鳞状上皮内病变及以下且HPV检测为阴性或其他高危型HPV阳性患者的分流作用

目的:探索p16/Ki67双染色法(简称双染)、HPV E6/E7 mRNA(简称E6/E7)检测对于宫颈细胞学检查(TCT)为低级别鳞状上皮内病变(LSIL)及以下且HPV检测为阴性或其他高危型HPV阳性患者的分流作用。方法:收集郑州大学第一附属医院妇科门诊就诊的TCT检查为LSIL及以下且HPV检测为阴性或其他高危型HPV阳性(不包括HPV16、18阳性)的414例患者的宫颈脱落细胞,分别进行双染检测、HPV E6/E7 mRNA检测,并行阴道镜下活检,以组织病理学诊断为“金标准”,采用灵敏度、特异度比较TCT+HPV+双染、TCT+HPV+E6/E7和TCT+HPV检测用于筛查高级别鳞状上皮内病变及以上(HSIL+)患者的效能。结果:根据病理结果,414例中150例为HSIL+。TCT+HPV+双染检出宫颈HSIL+病变的灵敏度、特异度分别为78.7%、50.4%,TCT+HPV+E6/E7分别为74.0%、26.1%,而TCT+HPV为90.0%、8.0%。TCT+HPV+双染和TCT+HPV+E6/E7检出HSIL+病变的灵敏度相近,均略低于TCT+HPV(P<0.05),而TCT+HPV+双染的特异度远高于其他2种方法(P<0.05)。结论:对于TCT检查为LSIL及以下且HPV阴性或其他高危型HPV阳性患者,采用p16/Ki67双染联合检测具有较高的灵敏度,且能显著提高特异度,其筛查效能优于TCT和HPV联合筛查。

Electrospun P(LLA-CL) Nanofibers Loaded with Vitamins E TPGS as Antibacterial Wound Dressing Materials

To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully incorporated into P(LLA-CL)nanofibers by electrospinning.Electron microscopy showed that the smooth cylindrical fibers were obtained,albeit with a small amount of beading visible for the vitamins-loaded fibers.The diameters of the P(LLA-CL)fibers decreased with the addition of vitamins.The incorporation of the vitamin E TPGS in the electrospun fibers was confirmed by Fourier transform infrared spectroscopy(FTIR).Moreover,X-ray diffraction(XRD)indicated that vitamin E TPGS existed in the amorphous physical form after electrospinning.Fibers containing vitamin E TPGS showed a sustained release profile over more than 100 h in vitro.Antibacterial tests demonstrated that fibers loaded with vitamin E TPGS were effective in inhibiting the growth of E.coli and S.aureus.MTT assay showed that the fibers could promote the proliferation of L929 fibroblasts.These results above demonstrate the potential of P(LLA-CL)/vitamins E TPGS(P/E)as advanced wound dressing materials.