[ Objectivel The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose. [ Method] 0.5 mmol/L FeSO4, CuSO4, MnSO4 and ZnSO4 solutions were added to the b...[ Objectivel The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose. [ Method] 0.5 mmol/L FeSO4, CuSO4, MnSO4 and ZnSO4 solutions were added to the basal culture media containing silkworm excrement and rice husk, and the effects of metal ions on activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngiiwere analyzed. [ Resultl The addi- tion of Zn2. and Mn~＊ to the basal culture media containing silkworm excrement and rice husk was beneficial to the improvement of the activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii, while the addition of Fe2＋ and Cu2. inhibited the activity of the cellulase. EConclu- sion I The research could provide theoretical references for improvement of utilization rate of cellulase in silkworm excrement and rice husk.展开更多
Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal speci...Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal species to degrade conventional and degradable plastic films. Plastics studied were oxo-degradable polyethylene (OXO-PE), UV-irradiated oxo-degradable polyethylene (UV-OXO-PE), polylactic acid (PLA) and conventional polyethylene (C-PE). The cultivation of P. ostreatus resulted in a reduction in the median of weight (78.2% - 80.2%) and volume (56.1% - 60.1%) of the substrate (wheat straw). Degradation of the plastics embodied was evidenced by a reduction in the median of the elongation at break (OXO-PE 475% to 109%, UV-OXO-PE 23% to 8%, PLA 596% to 398% and C-PE 505% to 304%) and an increase in the median of the carbonyl index (OXO-PE 0.062 to 0.114, UV-OXO-PE 0.098 to 0.145 and PLA 0.024 to 0.034). The Kruskal-Wallis test found no statistical difference (p = 0.384) between the medians of the biological efficiency for substrates containing plastics and the substrate without plastic. In conclusion, plastics embodied in the substrates used for cultivation of P. ostreatus are degraded and the degradation of these plastics does not affect the short term growth of P. ostreatus.展开更多
An indigenous and industrially important manganese peroxidase （MnP） isolated from solid state culture of Pleurotus ostreatus IBL-02 was 2.1-fold purified and immobilized using sol-gel matrix of trimethoxysilane and ...An indigenous and industrially important manganese peroxidase （MnP） isolated from solid state culture of Pleurotus ostreatus IBL-02 was 2.1-fold purified and immobilized using sol-gel matrix of trimethoxysilane and proplytetramethoxysilane. Lower KM, higher Vmax, hyper-activation, enhanced acidic and thermal stability up to 70 ℃, were the improved]enhanced catalytic features of the presently sol-gel immobilized MnP. A maximum of 100% decolorization was achieved within a short time period for the different real dyes containing textile industry effluents i.e. Nishat textile （NIT）, K＆N textile （KIT）, Sitara textile （SIT）, and Crescent textile （CRT）.展开更多
文摘[ Objectivel The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose. [ Method] 0.5 mmol/L FeSO4, CuSO4, MnSO4 and ZnSO4 solutions were added to the basal culture media containing silkworm excrement and rice husk, and the effects of metal ions on activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngiiwere analyzed. [ Resultl The addi- tion of Zn2. and Mn~＊ to the basal culture media containing silkworm excrement and rice husk was beneficial to the improvement of the activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii, while the addition of Fe2＋ and Cu2. inhibited the activity of the cellulase. EConclu- sion I The research could provide theoretical references for improvement of utilization rate of cellulase in silkworm excrement and rice husk.
文摘Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal species to degrade conventional and degradable plastic films. Plastics studied were oxo-degradable polyethylene (OXO-PE), UV-irradiated oxo-degradable polyethylene (UV-OXO-PE), polylactic acid (PLA) and conventional polyethylene (C-PE). The cultivation of P. ostreatus resulted in a reduction in the median of weight (78.2% - 80.2%) and volume (56.1% - 60.1%) of the substrate (wheat straw). Degradation of the plastics embodied was evidenced by a reduction in the median of the elongation at break (OXO-PE 475% to 109%, UV-OXO-PE 23% to 8%, PLA 596% to 398% and C-PE 505% to 304%) and an increase in the median of the carbonyl index (OXO-PE 0.062 to 0.114, UV-OXO-PE 0.098 to 0.145 and PLA 0.024 to 0.034). The Kruskal-Wallis test found no statistical difference (p = 0.384) between the medians of the biological efficiency for substrates containing plastics and the substrate without plastic. In conclusion, plastics embodied in the substrates used for cultivation of P. ostreatus are degraded and the degradation of these plastics does not affect the short term growth of P. ostreatus.
基金a part of a research project"the development of immobilized ligninolytic enzymes for industrial applications"financial support,Higher Education Commission, Pakistan
文摘An indigenous and industrially important manganese peroxidase （MnP） isolated from solid state culture of Pleurotus ostreatus IBL-02 was 2.1-fold purified and immobilized using sol-gel matrix of trimethoxysilane and proplytetramethoxysilane. Lower KM, higher Vmax, hyper-activation, enhanced acidic and thermal stability up to 70 ℃, were the improved]enhanced catalytic features of the presently sol-gel immobilized MnP. A maximum of 100% decolorization was achieved within a short time period for the different real dyes containing textile industry effluents i.e. Nishat textile （NIT）, K＆N textile （KIT）, Sitara textile （SIT）, and Crescent textile （CRT）.
