AIM： To determine if blockade of P-selectin in the isolated blood-perfused cold ex vivo rat liver model protects the liver from ischemia-reperfusion injury. METHODS： The effect of P-selectin blockade was assessed by...AIM： To determine if blockade of P-selectin in the isolated blood-perfused cold ex vivo rat liver model protects the liver from ischemia-reperfusion injury. METHODS： The effect of P-selectin blockade was assessed by employing an isolated blood-perfused cold ex vivo rat liver with or without P-selectin antibody treatment before and after 6 h of cold storage in University of Wisconsin solution. RESULTS： In our isolated blood-perfused rat liver model, pre-treatment with P-selectin antibody failed to protect the liver from ischemia-reperfusion injury, as judged by the elevated aspartate aminotransferase activity. In addition, P-selectin antibody treatment did not significantly reduced hepatic polymorphonuclear leukocyte accumulation after 120 min of perfusion. Histological evaluation of liver sections obtained at 120 min of perfusion showed significant oncotic necrosis in liver sections of both ischemic control and P-selectin antibody-treated groups. However, total bile production after 120 rain of perfusion was significantly greater in P-selectin antibody-treated livers, compared to control livers. No significant difference in P-selectin and ICAM-1 mRNAs and proteins, GSH, GSSG, and nuclear NF-kB was found between control and P-selectin antibody-treated livers. CONCLUSION： In conclusion, we have shown that blockade of P-selectin alone failed to reduced polymorphonuclear leukocyte accumulation in the liver and protect hepatocytes from ischemia-reperfusion injury in the isolated blood-perfused cold-ex vivo rat liver model.展开更多
Objective:To explore the correlation of serum soluble adhesion molecules P-selectin and ICAM-1 with change of the illness in patients with chronic hepatitis B.Methods: 119 patients with chronic hepatitis B were divide...Objective:To explore the correlation of serum soluble adhesion molecules P-selectin and ICAM-1 with change of the illness in patients with chronic hepatitis B.Methods: 119 patients with chronic hepatitis B were divided into chronic hepatitis B group (n=83, patients with normal chronic hepatitis B) and severe hepatitis B group (n=36, patients with severe hepatitis B) according to the disease severity, and 50 healthy volunteers who received physical examination in our hospital during the same period were chosen as normal control group. The differences in serum soluble adhesion molecules P-selectin and ICAM-1, hepatitis B viral load (HBV-DNA), liver function indexes (AST, ALT, TP and TBil) as well as liver fibrosis indexes (CⅣ, HA and LN) were compared among the three groups, and the inner link of serum P-selectin and ICAM-1 contents with the above disease indexes in patients with chronic hepatitis B was further analyzed.Results: Serum P-selectin contents of chronic hepatitis B group and severe hepatitis B group were higher than that of normal control group respectively while ICAM-1 contents were lower than that of normal control group respectively, and the changes in serum P-selectin and ICAM-1 contents intensified as the patients' condition worsened. Serum HBV-DNA levels of chronic hepatitis B group and severe hepatitis B group were higher than that of normal control group respectively, and serum HBV-DNA level of severe hepatitis B group was higher;serum AST, ALT and TBil levels of severe hepatitis B group were higher than those of chronic hepatitis B group and normal control group while TP level was lower than that of chronic hepatitis B group and normal control group;serum CⅣ, HA and LN contents of chronic hepatitis B group and severe hepatitis B group were higher than those of normal control group respectively, and serum CⅣ, HA and LN contents of severe hepatitis B group were higher. Correlation analysis revealed that serum P-selectin and ICAM-1 contents in patients with chronic hepatitis B were directly correlated with hepatitis B viral load, liver function and liver fibrosis degree.Conclusion: Serum P-selectin content abnormally increases while ICAM-1 content abnormally decreases in patients with chronic hepatitis B, and their serum contents can be used to measure the disease severity.展开更多
Based on the collected multiwavelength data, namely in the radio(NVSS, FIRST, RATAN-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blaza...Based on the collected multiwavelength data, namely in the radio(NVSS, FIRST, RATAN-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blazars of the Roma-BZCAT catalog. Using two machine learning methods, namely a combination of PCA with k-means clustering and Kohonen's self-organizing maps(SOMs), we have constructed an independent classification of the blazars(five classes) and compared the classes with the known Roma-BZCAT classification(FSRQs, BL Lacs, galaxy-dominated BL Lacs, and blazars of an uncertain type) as well as with the high synchrotron peaked(HSP) blazars from the 3HSP catalog and blazars from the TeVCat catalog. The obtained groups demonstrate concordance with the BL Lac/FSRQ classification along with a continuous character of the change in the properties. The group of HSP blazars stands out against the overall distribution. We examine the characteristics of the five groups and demonstrate distinctions in their spectral energy distribution shapes. The effectiveness of the clustering technique for objective analysis of multiparametric arrays of experimental data is demonstrated.展开更多
[Objectives] To screen the active components and core targets of Gastrodia elata Bl. based on network pharmacology, and further explore its potential molecular mechanism in preventing and treating chronic heart failur...[Objectives] To screen the active components and core targets of Gastrodia elata Bl. based on network pharmacology, and further explore its potential molecular mechanism in preventing and treating chronic heart failure (CHF).[Methods] The candidate active constituents of G. elata Bl. were screened by HERB, ETCM (Encyclopedia of Traditional Chinese Medicine) database, and bioinformatics analysis tool BATMAN-TCM platform, Swiss Target Prediction platform was used to predict compound targets, and CHF disease targets were searched in GeneCards and OMIM databases;the intersection targets were taken, and the String database and Cytoscape 3.10.0 software were used for protein-protein interaction (PPI) and topological analysis to obtain key active constituents and core target genes;the online tool bioinformatics platform was used for gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of common targets. Using the active constituent of G. elata Bl. as ligand and its core target as receptor, molecular docking visualization was carried out.[Results] 136 common targets of active constituents of G. elata Bl. and CHF were screened out, which mainly involved AGE-RAGE signaling pathway, lipid and atherosclerosis, interleukin-17 signaling pathway, non-alcoholic fatty liver, diabetic cardiomyopathy, cancer and other signaling pathways. The core target proteins mainly included albumin, catalytic subunit α activated by protein kinase CAMP, insulin gene, interleukin-1β, tumor necrosis factor, estrogen receptor 1, interferon γ, etc. The results of molecular docking showed that the ligand of the compound had good affinity with the target receptor.[Conclusions] G. elata Bl. prevents and treats CHF through multiple components, multiple targets, and multiple pathways. The potential mechanism of G. elata Bl. in treating CHF was preliminarily explored, providing a certain theoretical basis for subsequent research on its pharmacological material basis in treating CHF.展开更多
基金Supported by Grants from the American Liver Foundation, Naomi Judd Liver Scholar Award, The American Surgical Association Career Development Fellowship, and National Ⅰ
文摘AIM： To determine if blockade of P-selectin in the isolated blood-perfused cold ex vivo rat liver model protects the liver from ischemia-reperfusion injury. METHODS： The effect of P-selectin blockade was assessed by employing an isolated blood-perfused cold ex vivo rat liver with or without P-selectin antibody treatment before and after 6 h of cold storage in University of Wisconsin solution. RESULTS： In our isolated blood-perfused rat liver model, pre-treatment with P-selectin antibody failed to protect the liver from ischemia-reperfusion injury, as judged by the elevated aspartate aminotransferase activity. In addition, P-selectin antibody treatment did not significantly reduced hepatic polymorphonuclear leukocyte accumulation after 120 min of perfusion. Histological evaluation of liver sections obtained at 120 min of perfusion showed significant oncotic necrosis in liver sections of both ischemic control and P-selectin antibody-treated groups. However, total bile production after 120 rain of perfusion was significantly greater in P-selectin antibody-treated livers, compared to control livers. No significant difference in P-selectin and ICAM-1 mRNAs and proteins, GSH, GSSG, and nuclear NF-kB was found between control and P-selectin antibody-treated livers. CONCLUSION： In conclusion, we have shown that blockade of P-selectin alone failed to reduced polymorphonuclear leukocyte accumulation in the liver and protect hepatocytes from ischemia-reperfusion injury in the isolated blood-perfused cold-ex vivo rat liver model.
文摘Objective:To explore the correlation of serum soluble adhesion molecules P-selectin and ICAM-1 with change of the illness in patients with chronic hepatitis B.Methods: 119 patients with chronic hepatitis B were divided into chronic hepatitis B group (n=83, patients with normal chronic hepatitis B) and severe hepatitis B group (n=36, patients with severe hepatitis B) according to the disease severity, and 50 healthy volunteers who received physical examination in our hospital during the same period were chosen as normal control group. The differences in serum soluble adhesion molecules P-selectin and ICAM-1, hepatitis B viral load (HBV-DNA), liver function indexes (AST, ALT, TP and TBil) as well as liver fibrosis indexes (CⅣ, HA and LN) were compared among the three groups, and the inner link of serum P-selectin and ICAM-1 contents with the above disease indexes in patients with chronic hepatitis B was further analyzed.Results: Serum P-selectin contents of chronic hepatitis B group and severe hepatitis B group were higher than that of normal control group respectively while ICAM-1 contents were lower than that of normal control group respectively, and the changes in serum P-selectin and ICAM-1 contents intensified as the patients' condition worsened. Serum HBV-DNA levels of chronic hepatitis B group and severe hepatitis B group were higher than that of normal control group respectively, and serum HBV-DNA level of severe hepatitis B group was higher;serum AST, ALT and TBil levels of severe hepatitis B group were higher than those of chronic hepatitis B group and normal control group while TP level was lower than that of chronic hepatitis B group and normal control group;serum CⅣ, HA and LN contents of chronic hepatitis B group and severe hepatitis B group were higher than those of normal control group respectively, and serum CⅣ, HA and LN contents of severe hepatitis B group were higher. Correlation analysis revealed that serum P-selectin and ICAM-1 contents in patients with chronic hepatitis B were directly correlated with hepatitis B viral load, liver function and liver fibrosis degree.Conclusion: Serum P-selectin content abnormally increases while ICAM-1 content abnormally decreases in patients with chronic hepatitis B, and their serum contents can be used to measure the disease severity.
文摘Based on the collected multiwavelength data, namely in the radio(NVSS, FIRST, RATAN-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blazars of the Roma-BZCAT catalog. Using two machine learning methods, namely a combination of PCA with k-means clustering and Kohonen's self-organizing maps(SOMs), we have constructed an independent classification of the blazars(five classes) and compared the classes with the known Roma-BZCAT classification(FSRQs, BL Lacs, galaxy-dominated BL Lacs, and blazars of an uncertain type) as well as with the high synchrotron peaked(HSP) blazars from the 3HSP catalog and blazars from the TeVCat catalog. The obtained groups demonstrate concordance with the BL Lac/FSRQ classification along with a continuous character of the change in the properties. The group of HSP blazars stands out against the overall distribution. We examine the characteristics of the five groups and demonstrate distinctions in their spectral energy distribution shapes. The effectiveness of the clustering technique for objective analysis of multiparametric arrays of experimental data is demonstrated.
文摘[Objectives] To screen the active components and core targets of Gastrodia elata Bl. based on network pharmacology, and further explore its potential molecular mechanism in preventing and treating chronic heart failure (CHF).[Methods] The candidate active constituents of G. elata Bl. were screened by HERB, ETCM (Encyclopedia of Traditional Chinese Medicine) database, and bioinformatics analysis tool BATMAN-TCM platform, Swiss Target Prediction platform was used to predict compound targets, and CHF disease targets were searched in GeneCards and OMIM databases;the intersection targets were taken, and the String database and Cytoscape 3.10.0 software were used for protein-protein interaction (PPI) and topological analysis to obtain key active constituents and core target genes;the online tool bioinformatics platform was used for gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of common targets. Using the active constituent of G. elata Bl. as ligand and its core target as receptor, molecular docking visualization was carried out.[Results] 136 common targets of active constituents of G. elata Bl. and CHF were screened out, which mainly involved AGE-RAGE signaling pathway, lipid and atherosclerosis, interleukin-17 signaling pathway, non-alcoholic fatty liver, diabetic cardiomyopathy, cancer and other signaling pathways. The core target proteins mainly included albumin, catalytic subunit α activated by protein kinase CAMP, insulin gene, interleukin-1β, tumor necrosis factor, estrogen receptor 1, interferon γ, etc. The results of molecular docking showed that the ligand of the compound had good affinity with the target receptor.[Conclusions] G. elata Bl. prevents and treats CHF through multiple components, multiple targets, and multiple pathways. The potential mechanism of G. elata Bl. in treating CHF was preliminarily explored, providing a certain theoretical basis for subsequent research on its pharmacological material basis in treating CHF.
