Summary: To investigate the expression and clinical significance of p27kip1 protein in primary liver cancer, the expression of p27kip1 protein and the relationship with clinicopathological factors were studied in prim...Summary: To investigate the expression and clinical significance of p27kip1 protein in primary liver cancer, the expression of p27kip1 protein and the relationship with clinicopathological factors were studied in primary liver cancer by using SABC immunohistochemical staining in specimens of 40 cases of primary liver cancer and 20 cases of liver cirrihosis. Our results showed that positive expression rate of p27kip1 protein in primary liver cancer was 37.5 % (15/40), which was lower than that in benign lesion of liver 80.0 % (16/20, P<0.01). The expression level of p27kip1 protein in primary liver cancer showed significant differences in tumor size, Edmonson histological grade, portal invasion, lymph node metastasis, TNM stage (P<0.05, for all), but not significantly correlated with patient's age and histological types. Log rank test showed that the p27kip1 expression was significantly related with prognosis of the patients (P<0.05), and the prognosis of the patients with p27kip1 positive expression was markedly better than that of those with p27kip1 negative expression. It is concluded that the expression of p27kip1 was significantly related clinicopathological factors of primary liver cancer. p27kip1 protein may be used as a novel tumor marker for primary liver cancer.展开更多
Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of ea...Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV(SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast twohybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus.展开更多
Forkhead box protein P1（FOXP1）is a transcription factor belonging to the forkhead box（FOX）proteins,a family of transcriptional regulators sharing a highly conserved forkhead DNA-binding domain（Bacon and Rappold,2...Forkhead box protein P1（FOXP1）is a transcription factor belonging to the forkhead box（FOX）proteins,a family of transcriptional regulators sharing a highly conserved forkhead DNA-binding domain（Bacon and Rappold,2012）.Previous reports have proposed a role for FOXP1 in functionally regulating the central nervous system（CNS）,while mutations in FOXP1 have been implicated in cognitive abnormalities（Bacon and Rappold, 2012）.展开更多
目的:观察FOXP1蛋白在不同亚型的弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)组织中的表达,并探讨其与预后的关系。方法:回顾性分析2004年1月—2007年12月广西医科大学附属肿瘤医院诊断明确、病例资料齐全的DLBCL患者的...目的:观察FOXP1蛋白在不同亚型的弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)组织中的表达,并探讨其与预后的关系。方法:回顾性分析2004年1月—2007年12月广西医科大学附属肿瘤医院诊断明确、病例资料齐全的DLBCL患者的石蜡标本共86例,应用免疫组织化学法检测肿瘤组织中FOXP1蛋白表达情况,采用χ2检验比较FOXP1不同表达组与化疗后完全缓解率之间的关系。结果:86例DLBCL标本中,生发中心型47例,其中FOXP1阳性者10例(21.28%);非生发中心型39例,其中FOXP1阳性者31例(79.49%);2组患者的FOXP1表达率差异有统计学意义(P<0.05)。FOXP1阳性的41例患者中化疗后完全缓解(complete response,CR)者10例(24.39%),FOXP1阴性的45例患者中化疗后CR者29例(64.44%),2者CR率的差异有统计学意义(P<0.05)。FOXP1表达与患者的性别、年龄、临床分期、乳酸脱氢酶水平、PS评分、淋巴结外侵犯情况、B症状及有无巨大包块均无关。结论:FOXP1蛋白主要在非生发中心型DLBCL组织中表达,阳性表达的患者CR率低,可以利用FOXP1蛋白表达水平预测DLBCL的预后。展开更多
文摘Summary: To investigate the expression and clinical significance of p27kip1 protein in primary liver cancer, the expression of p27kip1 protein and the relationship with clinicopathological factors were studied in primary liver cancer by using SABC immunohistochemical staining in specimens of 40 cases of primary liver cancer and 20 cases of liver cirrihosis. Our results showed that positive expression rate of p27kip1 protein in primary liver cancer was 37.5 % (15/40), which was lower than that in benign lesion of liver 80.0 % (16/20, P<0.01). The expression level of p27kip1 protein in primary liver cancer showed significant differences in tumor size, Edmonson histological grade, portal invasion, lymph node metastasis, TNM stage (P<0.05, for all), but not significantly correlated with patient's age and histological types. Log rank test showed that the p27kip1 expression was significantly related with prognosis of the patients (P<0.05), and the prognosis of the patients with p27kip1 positive expression was markedly better than that of those with p27kip1 negative expression. It is concluded that the expression of p27kip1 was significantly related clinicopathological factors of primary liver cancer. p27kip1 protein may be used as a novel tumor marker for primary liver cancer.
基金supported by the grants from the National Natural Science Foundation of China(32072386 and 31801700)the Key Research and Development Project of Anhui Province,China(202004a06020013)the Postdoctoral Science Fund of Anhui Province,China(2019B360)。
文摘Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV(SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast twohybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus.
文摘Forkhead box protein P1（FOXP1）is a transcription factor belonging to the forkhead box（FOX）proteins,a family of transcriptional regulators sharing a highly conserved forkhead DNA-binding domain（Bacon and Rappold,2012）.Previous reports have proposed a role for FOXP1 in functionally regulating the central nervous system（CNS）,while mutations in FOXP1 have been implicated in cognitive abnormalities（Bacon and Rappold, 2012）.
文摘目的:观察FOXP1蛋白在不同亚型的弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)组织中的表达,并探讨其与预后的关系。方法:回顾性分析2004年1月—2007年12月广西医科大学附属肿瘤医院诊断明确、病例资料齐全的DLBCL患者的石蜡标本共86例,应用免疫组织化学法检测肿瘤组织中FOXP1蛋白表达情况,采用χ2检验比较FOXP1不同表达组与化疗后完全缓解率之间的关系。结果:86例DLBCL标本中,生发中心型47例,其中FOXP1阳性者10例(21.28%);非生发中心型39例,其中FOXP1阳性者31例(79.49%);2组患者的FOXP1表达率差异有统计学意义(P<0.05)。FOXP1阳性的41例患者中化疗后完全缓解(complete response,CR)者10例(24.39%),FOXP1阴性的45例患者中化疗后CR者29例(64.44%),2者CR率的差异有统计学意义(P<0.05)。FOXP1表达与患者的性别、年龄、临床分期、乳酸脱氢酶水平、PS评分、淋巴结外侵犯情况、B症状及有无巨大包块均无关。结论:FOXP1蛋白主要在非生发中心型DLBCL组织中表达,阳性表达的患者CR率低,可以利用FOXP1蛋白表达水平预测DLBCL的预后。
