Objective: To study cell cycle retardation, apoptosis and the expression of antioncogene p57kip2 by radioactive rays in nasopharyngeal carcinoma cells. Methods: Cell cycle retardation, apoptosis and cell survival rate...Objective: To study cell cycle retardation, apoptosis and the expression of antioncogene p57kip2 by radioactive rays in nasopharyngeal carcinoma cells. Methods: Cell cycle retardation, apoptosis and cell survival rate induced by radioac- tive rays were tested by the methods of flow cytometry and MTT method. The expression of antioncogene p57kip2 was detected by immunohistochemistry and Western blot. Results: After irradiation, G1 phase had no obvious retardation, S phase showed transient delay. There was a positive correlation between irradiation dosage and retardation strength in G2/M phase (P < 0.01). Peak value appeared at 24 h after 12 Gy irradiation, then decreased. There was a positive correlation between apop- tosis incidence and irradiation dosage or after-irradiation time extention (P < 0.01). There was a negative correlation between cell survival rate and irradiation dosage or apoptosis incidence (P < 0.01). The expression of p57kip2 protein was up-regulated along with the prolongation of time and dosage after irradiation (P < 0.01). Conclusion: G2/M phase arrest, apoptosis and the up-regulation of the expression of p57kip2 protein all can reflect predict the radiosensitivity of nasopharyngeal carcinoma cells.展开更多
目的探讨细胞S相激酶相关蛋白(S-phasekinase associated protein 2,Skp2)、p27蛋白与喉鳞状细胞癌(简称喉癌)各临床因素及预后的相关性。方法采用免疫组化SP法检测79例喉癌患者肿瘤组织的Skp2、p27表达。结果喉癌中Skp2高表达率(53.16%...目的探讨细胞S相激酶相关蛋白(S-phasekinase associated protein 2,Skp2)、p27蛋白与喉鳞状细胞癌(简称喉癌)各临床因素及预后的相关性。方法采用免疫组化SP法检测79例喉癌患者肿瘤组织的Skp2、p27表达。结果喉癌中Skp2高表达率(53.16%)显著高于正常喉组织(0%,P<0.05);喉癌Skp2蛋白低表达组的5年生存率(72.18%)显著高于高表达组(44.17%,P<0.01)。p27蛋白在喉癌和癌旁喉组织中的高表达率分别为30.38%和90%,差异具有显著性(P<0.05);喉癌p27蛋白高表达组的5年生存率(72.98%)显著高于低表达组(51.13%,P<0.01)。将Skp2和p27结合分析,Skp2高表达并p27低表达组的5年生存率最低,与另一组相比具有显著性差异(P=0.001)。结论Skp2蛋白通过降解靶蛋白p27可能在喉癌发生、发展中发挥重要作用。展开更多
Background: Esophageal squamous cell carcinoma(ESCC) is a leading cause of cancer?related death, and new prognostic biomarkers are urgently needed. Apoptosis?stimulating P53?binding protein 1(ASPP1) and 2(ASPP2) have ...Background: Esophageal squamous cell carcinoma(ESCC) is a leading cause of cancer?related death, and new prognostic biomarkers are urgently needed. Apoptosis?stimulating P53?binding protein 1(ASPP1) and 2(ASPP2) have been reported to play important roles in the development, progression, metastasis, and prognosis of cancers, but their roles in ESCC have not been elucidated. In this study, we examined the expression of ASPP1 and ASPP2 in ESCC to evaluate their prognostic values.Methods: The protein expression of ASPP1, ASPP2, and P53 in 175 specimens of ESCC was detected using immuno?histochemical staining; their expression in cancerous and noncancerous tissues was scored according to the stain?ing intensity and the percentage of stained cells. The associations of ASPP1, ASPP2, and P53 with clinicopathologic parameters, overall survival(OS), and disease?free survival(DFS) were analyzed.Results: The protein expression levels of ASPP2 and P53 were significantly higher in cancerous tissues than in paired noncancerous tissues(P < 0.001), whereas the expression levels of ASPP1 in the two groups were similar. In ESCCs, ASPP1 expression was significantly associated with histological differentiation(P = 0.002) and invasive depth(P = 0.014); ASPP2 expression was associated with age(P = 0.029) and histological differentiation(P < 0.001); and P53 expression was associated with age(P and P53 expression. Survival an= 0.021) and tumor size(P alysis revealed that high AS= 0.040). No correlations were found between ASPP1, ASPP2,PP2 expression was significantly associated with increased 5?year OS(P = 0.001) and DFS rates(P ate of ESCC patients(= 0.010) and that high P53 expression was significantly associated with a reduced 5?year DFS rP atio(HR): 0.541, 9= 0.015). Multivariate Cox analysis indicated that ASPP2 was an inde?pendent predictor of OS [hazard r5% confidence interval(CI) 0.363–0.804] and DFS(HR: 0.599, 95% CI 0.404–0.888) of ESCC patients and that P53 was an independent predictor of DFS(HR: 2.161, 95% CI 1.100–4.245).Conclusions: ASPP1 might be involved in the progression of ESCC, and ASPP2 was a potential prognostic biomarker of ESCC and should be evaluated in future studies.展开更多
AIM:To investigate whether upregulation of apoptosisstimulating p53 protein 2(ASPP2)expression could alleviate the development of proliferative vitreoretinopathy(PVR)in a rat model.METHODS:ASPP2-lentivirus or scramble...AIM:To investigate whether upregulation of apoptosisstimulating p53 protein 2(ASPP2)expression could alleviate the development of proliferative vitreoretinopathy(PVR)in a rat model.METHODS:ASPP2-lentivirus or scrambled-lentivirus were transfected into ARPE-19 cells,followed with measurements of cell cytotoxicity by cell counting kit-8 assay.ASPP2 upregulation was confirmed by Western blotting and immunocytochemistry.Then ARPE-19 cells pretreated with ASPP2-lentivirus were intravitreally injected to Brown Norway rats to induce PVR models.PVR development and retinal function were evaluated by retinal photography and electroretinography,respectively.Finally,epithelial-mesenchymal transition as well as autophagy were investigated in rats’retinas via Western blotting.RESULTS:Protein expression of ASPP2 was significantly upregulated by ASPP2-lentivirus transfection in ARPE-19 cells.The development and progression of PVR were impeded significantly in rats with intravitreal injection of ARPE-19 cells pretreated with ASPP2-lentivirus.Accordingly,retinal functions were less affected and PVR grades were much lower in rats with ASPP2-lentivirus compared to scrambledlentivirus treatment.Moreover,epithelial-mesenchymal transition and autophagy markers were decreased in the retinas of rats treated with ASPP2-lentivirus.CONCLUSION:ASPP2-lentivirus transfected to ARPE-19 cells mitigates the progression of PVR in rat models,which might be partly through reduced autophagy and attenuated epithelial-mesenchymal transition.ASPP2 might stand as a new approach for PVR treatment in the future.展开更多
文摘Objective: To study cell cycle retardation, apoptosis and the expression of antioncogene p57kip2 by radioactive rays in nasopharyngeal carcinoma cells. Methods: Cell cycle retardation, apoptosis and cell survival rate induced by radioac- tive rays were tested by the methods of flow cytometry and MTT method. The expression of antioncogene p57kip2 was detected by immunohistochemistry and Western blot. Results: After irradiation, G1 phase had no obvious retardation, S phase showed transient delay. There was a positive correlation between irradiation dosage and retardation strength in G2/M phase (P < 0.01). Peak value appeared at 24 h after 12 Gy irradiation, then decreased. There was a positive correlation between apop- tosis incidence and irradiation dosage or after-irradiation time extention (P < 0.01). There was a negative correlation between cell survival rate and irradiation dosage or apoptosis incidence (P < 0.01). The expression of p57kip2 protein was up-regulated along with the prolongation of time and dosage after irradiation (P < 0.01). Conclusion: G2/M phase arrest, apoptosis and the up-regulation of the expression of p57kip2 protein all can reflect predict the radiosensitivity of nasopharyngeal carcinoma cells.
文摘目的探讨细胞S相激酶相关蛋白(S-phasekinase associated protein 2,Skp2)、p27蛋白与喉鳞状细胞癌(简称喉癌)各临床因素及预后的相关性。方法采用免疫组化SP法检测79例喉癌患者肿瘤组织的Skp2、p27表达。结果喉癌中Skp2高表达率(53.16%)显著高于正常喉组织(0%,P<0.05);喉癌Skp2蛋白低表达组的5年生存率(72.18%)显著高于高表达组(44.17%,P<0.01)。p27蛋白在喉癌和癌旁喉组织中的高表达率分别为30.38%和90%,差异具有显著性(P<0.05);喉癌p27蛋白高表达组的5年生存率(72.98%)显著高于低表达组(51.13%,P<0.01)。将Skp2和p27结合分析,Skp2高表达并p27低表达组的5年生存率最低,与另一组相比具有显著性差异(P=0.001)。结论Skp2蛋白通过降解靶蛋白p27可能在喉癌发生、发展中发挥重要作用。
基金supported by grants from the Research Fund of Guangdong Esophageal Cancer Institute of China(Grant No:M201412 for H-YW)the Research Fund of the State Key Laboratory of Oncology in South China(to H-YW)
文摘Background: Esophageal squamous cell carcinoma(ESCC) is a leading cause of cancer?related death, and new prognostic biomarkers are urgently needed. Apoptosis?stimulating P53?binding protein 1(ASPP1) and 2(ASPP2) have been reported to play important roles in the development, progression, metastasis, and prognosis of cancers, but their roles in ESCC have not been elucidated. In this study, we examined the expression of ASPP1 and ASPP2 in ESCC to evaluate their prognostic values.Methods: The protein expression of ASPP1, ASPP2, and P53 in 175 specimens of ESCC was detected using immuno?histochemical staining; their expression in cancerous and noncancerous tissues was scored according to the stain?ing intensity and the percentage of stained cells. The associations of ASPP1, ASPP2, and P53 with clinicopathologic parameters, overall survival(OS), and disease?free survival(DFS) were analyzed.Results: The protein expression levels of ASPP2 and P53 were significantly higher in cancerous tissues than in paired noncancerous tissues(P < 0.001), whereas the expression levels of ASPP1 in the two groups were similar. In ESCCs, ASPP1 expression was significantly associated with histological differentiation(P = 0.002) and invasive depth(P = 0.014); ASPP2 expression was associated with age(P = 0.029) and histological differentiation(P < 0.001); and P53 expression was associated with age(P and P53 expression. Survival an= 0.021) and tumor size(P alysis revealed that high AS= 0.040). No correlations were found between ASPP1, ASPP2,PP2 expression was significantly associated with increased 5?year OS(P = 0.001) and DFS rates(P ate of ESCC patients(= 0.010) and that high P53 expression was significantly associated with a reduced 5?year DFS rP atio(HR): 0.541, 9= 0.015). Multivariate Cox analysis indicated that ASPP2 was an inde?pendent predictor of OS [hazard r5% confidence interval(CI) 0.363–0.804] and DFS(HR: 0.599, 95% CI 0.404–0.888) of ESCC patients and that P53 was an independent predictor of DFS(HR: 2.161, 95% CI 1.100–4.245).Conclusions: ASPP1 might be involved in the progression of ESCC, and ASPP2 was a potential prognostic biomarker of ESCC and should be evaluated in future studies.
基金National Natural Science Foundation of China(No.81800827).
文摘AIM:To investigate whether upregulation of apoptosisstimulating p53 protein 2(ASPP2)expression could alleviate the development of proliferative vitreoretinopathy(PVR)in a rat model.METHODS:ASPP2-lentivirus or scrambled-lentivirus were transfected into ARPE-19 cells,followed with measurements of cell cytotoxicity by cell counting kit-8 assay.ASPP2 upregulation was confirmed by Western blotting and immunocytochemistry.Then ARPE-19 cells pretreated with ASPP2-lentivirus were intravitreally injected to Brown Norway rats to induce PVR models.PVR development and retinal function were evaluated by retinal photography and electroretinography,respectively.Finally,epithelial-mesenchymal transition as well as autophagy were investigated in rats’retinas via Western blotting.RESULTS:Protein expression of ASPP2 was significantly upregulated by ASPP2-lentivirus transfection in ARPE-19 cells.The development and progression of PVR were impeded significantly in rats with intravitreal injection of ARPE-19 cells pretreated with ASPP2-lentivirus.Accordingly,retinal functions were less affected and PVR grades were much lower in rats with ASPP2-lentivirus compared to scrambledlentivirus treatment.Moreover,epithelial-mesenchymal transition and autophagy markers were decreased in the retinas of rats treated with ASPP2-lentivirus.CONCLUSION:ASPP2-lentivirus transfected to ARPE-19 cells mitigates the progression of PVR in rat models,which might be partly through reduced autophagy and attenuated epithelial-mesenchymal transition.ASPP2 might stand as a new approach for PVR treatment in the future.
