P300 change and cognitive behavioral therapy in subjects with Internet addiction disorder A 3-month follow-up study

Event-related potential studies of cognitive function in addiction behaviors have focused on the P300 event-related potential component. The current study investigated the association between P300 component and Internet addiction disorder. We found that individuals with Internet addiction disorder exhibited significantly longer P300 latencies than controls （N2： P = 0.035; P3a： P = 0.031 P3b： P = 0.043） and similar P300 amplitudes compared to control participants. After 3 months of cognitive behavioral therapy, P300 latencies decreased significantly in the P3a and P3b （P3a： P = 0.045; P3b： P = 0.062）. These results suggest that deficits in cognitive function may be involved in Internet addiction disorder, and that clinical psychological treatment may be effective.

Correlation of cognitive function with acetylcholinesterase activity and P300 event-related potential of patients with type 2 diabetes mellitus

BACKGROUND： At present, central cholinergic neuron system is regarded the most major structural basis of cognitive function. Changes in structure of cholinergic neuron system of brain and receptor expression after brain injury can cause cognitive impairment. OBJECTIVE＂ To comparatively observe the intelligence quotient （IQ）, latent period and wave amplitude of P300 event-related potential and the difference of activity of acetylcholinesterase （ACHE） in blood and cerebrospinal fluid between patients with type 2 diabetes mellitus and with non-diabetes mellitus, and analyze the correlation of IQ of cognitive impairment patients with diabetes mellitus with AChE activity, latent period and wave amplitude of P300 event-related potential in cerebrospinal fluid. DESIGN： Correlation analysis of contrast observation SETTING： Department of Endocrinology, Affiliated Hospital of Binzhou Medical College PARTICIPANTS： Totally 32 patients with type 2 diabetes mellitus who received the treatment in the Department of Endocrinology, Affiliated Hospital of Binzhou Medical College between April 2004 and April 2005 were recruited, serving as diabetes mellitus group. They, including 19 male and 13 female, aged 49 to 73 years, with disease course of 4 to 11 years, all met the diagnostic criteria of diabetes mellitus revised by World Health Organization in 1999. Another 30 patients with non-diabetes mellitus who homeochronously underwent lumbar anesthesia in the Department of Surgery and Department of Gynecology were recruited, serving as non-diabetes mellitus group. The 30 patients included 18 male and 12 female, and their age ranged from 46 to 71 years. Informed consents of detected items were obtained from the involved patients. METHODS： ① Evaluation,on IQ： The IQ of involved subjects was evaluated with Chinese Version of the Wechsler Adult Intelligence Scale revised by Gong Yao-xian （WAIS-RC）. WAIS-RC included 6 verbal subscales and 5 performance subscales. The test scores of the 11 subscales integrated into the scores of the whole scale, and the scores on the WAIS-RC included verbal IQ （VlQ）, performance IQ （PIQ） and full scale IQ （FIQ）. FIQ ≤79 scores indicated low IQ and FIQ≤69 indicated intelligence impairment. ② Detection of P300 wave： P300 wave was detected with evoked potential instrument （MYTOPRO, Italian）, and data of latent period and amplitude of P300 event-related potential were automatically shown by computer. ③ Detection of AChE activity in blood and cerebrospinal fluid： Activity of AChE of blood and cerebrospinal fluid was measured with biochemical methods by using CORNING-560 autoanalyzer.④Correlation analysis： Correlation of FIQ with AChE of cerebrospinal fluid and P300 wave of patients with type 2 diabetes mellitus was analyzed, t test was used in intergroup comparison and linear correlation analysis for relevant treatment. MAIN OUTCOME MEASURES： ① Comparison of IQ, latent period and wave amplitude of P300 wave as well as the activity of AChE between two groups. ② Analysis on the correlation of FIQ of patients with type 2 diabetes mellitus with AChE of cerebrospinal fluid and P300 wave. RESULTS： Thirty-two patients with diabetes mellitus and 30 non-diabetes mellitus participated in the result analysis. ①Comparison of IQ, latent period and wave amplitude of P300 wave as well as the activity of AChE between two groups： The scores of VIP, PIQ and FIQ of patients with type 2 diabetes mellitus were （97.4±10.4）. （92.6±8.4） and （95.2±9.7） scores, respectively; and those of patients with non-diabetes mellitus were （104.7±9.6）, （102.5±8.5）and（102.7±8.9） scores, respectively, and P 〈 0.05-0.01 was set in intergroup comparison. The latent period of P300 wave at points Fz , Cz and Pz of patients with type 2 diabetes mellitus was （370.8±41.8）.（371.5±39.1）and （375.1±43.1） ms, respectively, and that of patients with non-diabetes mellitus was （ 332.1 ±28.3 ）, （335.7 ±29.4）and （339.7 ±27.3） ms, respectively, and P 〈 0.01 was set in intergroup comparison; Wave amplitude of P300 of patients with type 2 diabetes mellitus was （8.6±4.1）,（8.6±4.0） and （7.7±4.0） μV, respectively and that of patients with non-diabetes mellitus was （11.9±4.1）,（11.5±4.4） and （10.9±5.0） μV, respectively , and P 〈 0.05-0.01 was set in intergroup comparison; The level of AChE in blood and cerebrospinal fluid of patients with type 2 diabetes mellitus was （235.61 ±50.34）and （17.89±4.46） μkat/L, respectively, which was significantly higher than that of patients with non-diabetes mellitus [（205.03±44.15）and （14.63±0.48） μkat /L, respectively], and P 〈 0.05-0.01 was set in the intergroup comparison. ② Correlation of FIQ value of patients with type 2 diabetes mellitus with AChE of cerebrospinal fluid and P300 wave： The value of FIQ was significantly negatively correlated with the AChE activity of cerebrospinal fluid （r=-0.588 1, P 〈 0.01 ）, significantly negatively correlated with the latent period at points Fz. C and Pz of P300 wave （r= -0.700 5, -0.689 4, -0.688 5, P 〈 0.01 ）, and significantly positively correlated with the amplitude at points Fz . Cz and Pz of P300 wave（r= 0.607 4,0.616 1,0.592 0,P 〈 0.01 ）. CONCLUSION： ① Cognitive impairment of patients with type 2 diabetes mellitus might be related to the increase of activity of AChE in cerebrospinal fluid. ②Combined application of examination of P300 wave and evaluation of IQ is more useful in deciding the state of cognitive function of patients with type 2 diabetes mellitus.

THE EFFECT OF ELECTROACUPUNCTURE ON AUDITORY P300 POTENTIAL IN MONGOLISM CASES

In treating children with mongolism(Down’s syndrome)with electroacupuncture,thelatent period of P3 is shortened,which shows that the cerebral reaction to externalstimulus in classification,encoding and recognition is accelerated.Along with the elevatedP3 amplitude,the cerebrum is more capable of processing the external information,showing improvement of the mental state.

ACUPUNCTURE COMBINED WITH LANGUAGE TRAINING FOR TREATMENT OF MOTOR APHASIA CAUSED BY ISCHEMIC APOPLEXY

Objective To observe therapeutic effects of acupuncture combined with language training on aphasia induced by ischemic apoplexy and investigate the mechanisms. Methods 60 patients were randomly divided into a treatment group treated by acupuncture associated with language training and a control group treated by simple language training. Tongue-acupuncture was applied as the main therapy, and language training included speech organ training, mouth-shape and voice training, spoken language expression training and practical communication ability training. According to Aphosio Bottery of Chinese （ABC）, language ex- amination evaluation was made, and event related potential （P300） was detected before and after treatment. Results The therapeutic effect in the treatment group was significantly better than that in the control group （P〈O. 05）, and the scores of ABC items including information content, speech fluency, repetition, vocabula- ry denomination, color naming, response denomination, confirmation or negation, acoustic recognition and carrying out instruction in the treatment group were obviously higher than those in the control group （P〈0.05）. After one course of treatment, the latencies of N2 and P3 waves in P300 were significantly short- ened and the amplitude of P3 was significantly elevated in the treatment group, compared with those in the control group （P〈0.05）. Conclusion Acupuncture combined with language training provided remarkable therapeutic effects in treating cerebral infarction-induced motor aphasia, and it was better than simple lan- guage training. The results of enhancing of the ABC scores including information content, speech fluency, repetition, vocabulary denomination, color naming, response denomination, confirmation or negation, acous- tic recognition and carrying out instruction, and shortening of the latencies of N2 and P3 waves in P300 and ele- vation of P3 amplitude may be taken as the indices for evaluating and anticipating clinical therapeutic effects of the therapy for treating the disease, which may also be some of the mechanisms.

A Clinical Investigation on Zhi Ling Tang for Treatment of Senile Dementia

Zhi Ling Tang (ZLT [symbol: see text], a TCM prescription designed for replenishing essence, supplementing marrow, invigorating qi, warming yang, removing blood stasis and phlegm, tonifying the brain, and invigorating mental activity) was used in 32 cases of senile dementia (SD). After treatment, the levels of serum cholesterol (Tch), triglyceride (TG), and plasma lipid peroxides (LPO) were lowered; the content of high density lipoprotein (HDL) and the activity of superoxide dismutase (SOD) in RBC significantly elevated, the cerebral blood flow was increased; latent period of P300 and P3 waves was shortened while the amplitude of P3 elevated; and topographic electroencephalogram, revised Hasegawa dementia scales (HDS) scores (P

Clinical Study on Treatment of Senile Vascular Dementia by Acupuncture

63 patients with senile vascular dementia were randomly divided into the treatment group (treated by acupuncture) and the control group (treated with piracetam). The authors observed the changes in the score of Hasegawa's dementia scale (HDS), p300, rheoencephalogram, topographic EEG, superoxide dismutase (SOD) activity in erythrocytes, and lipid peroxide (LPO) level in plasma before and after treatment. The statistical data showed that the total effective rate in the treatment group (80.6%) was significantly higher than that in the control group (25%), and the differences in the observed indexes before and after treatment were significant (P 0.05), indicating that the acupuncture treatment was superior in immediate therapeutic effect on senile vascular dementia to drug treatment.

女性抑郁症首次发病患者认知事件相关电位P300与脑灰质体积的相关分析

目的 探讨首次发病未服药女性抑郁症患者脑灰质体积变化特点,以及与认知事件相关电位P300的相关分析.方法 对25例18 ～58岁首次发病未服药女性抑郁症患者（患者组）和28名健康对照者（对照组）行头部3D结构MRI扫描,并对患者组进行P300检查.结果 患者组较对照组右侧额下回岛盖部、右侧中央前回灰质体积减小.患者组中P3、N2潜伏期均与双侧壳核、右侧颞中回体积呈正相关（均P ＜0.01）;P3潜伏期与左侧颞中回、左侧额中回、左侧梭状回体积呈正相关（均P＜0.01）,而P3波幅与左侧角回、右侧额上回体积呈负相关（均P＜0.01）;N2潜伏期与左侧后扣带回、右侧梭状回、枕叶、左侧小脑体积呈正相关（均P＜0.01）;患者组P300潜伏期和波幅与汉密尔顿抑郁量表的总分和各因子分无相关性.结论 首次发病未服药女性抑郁症患者存在特征性脑灰质体积异常,其P300可能与多个脑灰质区域相关,尤其与颞叶、基底神经节的关系最明显.

药物滥用性头痛患者认知功能及事件相关电位P300的研究

目的探讨药物滥用性头痛(MOH)患者认知功能及认知电位的变化情况。方法MOH患者(观察组)及对照组各30例,两组均进行P300、简易精神状态量表(MMSE)检测。采用SPSS 10.0软件进行数据处理,P300潜伏期、波幅比较采用t检验。结果两组P300靶刺激中P3潜伏期、波幅比较均差异有统计学意义:观察组P3潜伏期[分别为(393.70±42.60)ms]较对照组[(331.05±22.35)ms]延长,差异有统计学意义(t=7.13,P<0.05);观察组P3波幅[(3.40±2.30)u V]较对照组[(6.20±2.40)u V]明显降低,差异也有统计学意义(t=4.59,P<0.05)。两组MMSE评分比较,观察组总分、定向力、记忆力、计算力、回忆力、语言能力[(24.00±2.21)分,(8.15±0.86)分,(2.88±0.23)分,(3.07±1.04)分,(1.72±0.89)分,(8.42±1.33)分]低于对照组[分别为(27.07±2.06)分,(9.28±1.03)分,(3.01±0.26)分,(3.25±1.20)分,(1.92±0.90)分,(9.69±1.44)分],总分、定向力、语言能力评分差异有统计学意义(t=3.66,3.19,3.34;P<0.05)。结论 MOH患者存在轻度的认知功能障碍,认知电位P300为MOH患者认知功能障碍的诊断提供一客观指标。

精神发育迟滞患儿的事件相关电位视觉P300的特征及与智商的相关性

目的 探讨精神发育迟滞（MR）患儿的事件相关电位视觉P300的特征以及智商与视觉P300的潜伏期和波幅的关系.方法 选择2012年10月至2013年4月本院康复科门诊就诊及住院行康复治疗的MR患儿30例为MR组,另选同期本院儿童保健科体检的性别、年龄相匹配正常健康儿童30例为正常对照组.两组对象均在本院儿童保健科的智力测试室行韦氏智力测试,同时进行视觉P300的检测,比较两组P300的潜伏期和波幅的变化,并行P300的潜伏期和波幅值与言语智商（VIQ）、操作智商（PIQ）及总智商（FIQ）相关分析.结果 与正常对照组相比,MR组儿童事件相关电位视觉P300波的潜伏期延长,波幅降低,其差异有统计学意义（P＜0.05）.MR组儿童事件相关电位视觉P300的潜伏期与智商呈负相关（P＜0.05）.P300的波幅与IQ无相关（P＞0.05）.结论 事件相关电位视觉P300的潜伏期和波幅可客观地反映精神发育迟滞儿童认知功能的变化,可作为评价MR患儿认知功能的客观指标之一,具有协助诊断的意义.

精神分裂症患者认知功能、听觉事件诱发电位P300和脑灰质体积的相关分析

目的 分析经抗精神病药治疗后病情基本稳定但仍残留部分症状的非急性期精神分裂症患者认知功能、听觉事件诱发电位P300和脑灰质体积的相关性.方法 采用听觉事件诱发电位P300、神经心理认知测验对18例非急性期精神分裂症患者（患者组）和18名健康对照者（对照组）进行认知功能检测;采用PANSS和临床总体印象量表（Clinical Global Impression Scale-Severity,CGI-S）评定患者的精神症状,锥体外系副反应量表（Extrapyramidal Side Effects Scale,SAS）、不自主运动量表（Abnormal Involuntary Movement Scale,AIMS）和静坐不能量表（Barnes Akathisia Rating Scale,BARS）评定既往使用抗精神病药的不良反应情况.利用磁共振和脑诱发电位仪分别对患者和对照者进行脑部影像学、神经电生理检查.结果 神经心理认知功能测验中持续性操作测验（Continuous Performance Test,CPT）两位数和三位数测验患者组（2.5±1.2、2.1±1.2）较对照组（3.5±0.6、2.9±0.8）评分低（t=-3.088、-2.563,均P＜0.05）;Stroop色词干扰测试的单词、颜色和色词测试总分患者组（77.1 ±16.6、32.4±7.3和32.4±7.3）均较对照组（89.3±8.9、41.8±6.8、41.8±6.8）评分低（t=-2.733、-4.015、-4.015,均P＜0.05）;沟槽钉板测验利手和非利手患者组（69.9±10.9、77.3±11.4）较对照组（56.7±7.1、68.7±10.8）完成测试时间延长（t=-4.015、2.313,均P＜0.05）;颜色连线2患者组（46.3±18.1）较对照组（37.4±10.6）测试完成时间长（=4.638,P＜0.05）;范畴流利测验患者组（11.8±5.1）较对照组（15.6±3.5）评分低（t=-2.593,P＜0.05）,2组间比较差异有统计学意义.患者组的P300潜伏期N2（237.1±19.6）与对照组（217.6±26.6）比较延长（=2.966,P＜0.05）,波幅差异无统计学意义.P300波幅与迷宫测试、颜色连线及威斯康星卡片分类测验（Wisconsin Card Sorting Test,WCST）正确数呈正相关（r=0.493、0.527、0.512,均P＜0.05）,与沟槽钉板、WCST错误数呈负相关（r=-0.571、-0.512,均P＜0.05）.患者组的内侧前额叶、右侧颞叶和小脑部分区域的灰质体积较对照组下降,差异有统计学意义,P300潜伏期与局部脑灰质体积（右颞叶的颞上回和颞中回、右后叶小脑扁桃体、右侧小脑后叶、下半小叶）呈负相关（均P＜0.01）.结论 非急性期精神分裂症患者的认知功能存在广泛损害.患者内侧前额叶、颞叶、小脑灰质体积异常,且右侧颞叶、右侧小脑局部灰质体积与P300潜伏期相关,提示颞叶、小脑等区域可能是P300异常的脑结构和功能基础.

Citric acid reduces the decline in P300 amplitude induced by acute alcohol consumption in healthy adults

Event-related potential (ERP) is a reliable neuroelectric measure of brain activity that helps to confirm the assessment of mental status and cognitive impairment. Many studies have reported that alcoholics show a significantly lower ERP P300 amplitude than the norm. In the present study, ERP P300 waves were measured to evaluate the effect of citric acid on cognitive function during excessive alcohol consumption in healthy adults. Five volunteers were selected through clinical interview, physical examination, and psychiatric assessment for participation in this study. In a double-blind placebo-controlled before-after design, each subject was treated with 5 ml/kg body weight alcohol, 5 ml/kg body weight alcohol and 1 mg citric acid, or a placebo on three separate occasions, one week apart. ERP P300, blood biochemical indicators, blood alcohol concentrations (BACs) and acetaldehyde concentrations were assessed. Repeated measure analysis of variance (ANOVA) with a within-subjects factor was used to evaluate differences in blood biochemical indicators, BACs, blood acetaldehyde concentrations, and ERP P300 in the three sessions of assessments. Several blood biochemical indicators showed significant differences between treatments, including the levels of cholinesterase (CHE), total bile acid (TBA), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), and glycylproline dipeptidyl aminopeptidase (GPDA). BACs after consumption of alcohol alone or citric acid with al- cohol were significantly higher compared to those after placebo treatment (P<0.05). There were no significant dif- ferences in blood acetaldehyde concentrations between the treatments. The P300 amplitudes on the frontal (Fz), central (Cz), and parietal (Pz) regions of the scalp after consumption of alcohol were significantly lower than those after consumption of the placebo or citric acid with alcohol (P<0.05), while there were no significant differences between the latter two treatments. The results of this study suggest that citric acid could reduce the decline in ERP P300 amplitude and cognitive ability induced by acute alcohol consumption. It may also affect some blood biochemical indicators, but the specific mechanisms need further research.

A hybrid brain-computer interface control strategy in a virtual environment

This paper presents a hybrid brain-computer interface (BCI) control strategy,the goal of which is to expand control functions of a conventional motor imagery or a P300 potential based BCI in a virtual environment.The hybrid control strategy utilizes P300 potential to control virtual devices and motor imagery related sensorimotor rhythms to navigate in the virtual world.The two electroencephalography (EEG) patterns serve as source signals for different control functions in their corresponding system states,and state switch is achieved in a sequential manner.In the current system,imagination of left/right hand movement was translated into turning left/right in the virtual apartment continuously,while P300 potentials were mapped to discrete virtual device control commands using a five-oddball paradigm.The combination of motor imagery and P300 patterns in one BCI system for virtual environment control was tested and the results were compared with those of a single motor imagery or P300-based BCI.Subjects obtained similar performances in the hybrid and single control tasks,which indicates the hybrid control strategy works well in the virtual environment.

Assessment of cognitive dysfunction in traumatic brain injury patients:a review

Traumatic brain injury(TBI)is one of the major causes of human mortality and morbidity in the world.Brain injury could affect the core of a person’s being–their thinking,memory,personality and behaviour.Electrophysiological markers from the human electroencephalogram and brain imaging provide a rich source of data which helps to elucidate specific processing impairments in TBI patients.To assess the cognitive and social function in traumatic brain injury patients,this review will focus on some of methods for assessing the disabling cognitive and social function deficits induced by TBI.There are many new technologies available to address TBI and recognition related questions.Integration of the various techniques will help to facilitate our comprehending of TBI,cognitive function and social function,and improve treatment and rehabilitation.

Clinical study on Jin's three-needle therapy for post-stroke cognitive impairment

Objective:To observe the clinical efficacy of Jin's three-needle therapy on post-stroke cognitive impairment(PSCI)and the effect on neuroelectrophysiology(event-related potentials).Methods:A total of 60 PSCI patients were selected and divided into a treatment group and a control group according to the method of random number table,with 30 cases in each group.The patients in the control group received routine treatment while the patients in the treatment group received additional Jin's three-needle therapy.The treatment for both groups lasted four weeks.Mini-mental state examination(MMSE)and Montreal cognitive assessment(MoCA)scores as well as amplitude and latency of potential 300(P300)were adopted to compare the between-group results before and after treatment.Results:Before treatment,there were no significant differences(all P>0.05)in MMSE and MoCA scores,P300 latency and P300 amplitude between the two groups.After 4 weeks of treatment,the MMSE and MoCA scores and P300 amplitudes were improved in both groups,and the P300 latencies became shorter.The results showed significant intra-group and between-group differences(all P<0.05).Conclusion:Based on the routine treatment,Jin's three-needle therapy is effective for PSCI.The mechanism is probably through its regulation on the patients'neuroelectrophysiology.

尼莫地平联合卡巴拉汀治疗老年血管性痴呆的临床疗效与不良反应

目的 观察尼莫地平联合卡巴拉汀治疗老年血管性痴呆的临床疗效与不良反应,为该疾病提供有效的治疗方法.方法 将151例老年血管性痴呆患者采用随机数字表法分为观察组78例和对照组73例.对照组被给予尼莫地平治疗,观察组被给予尼莫地平联合卡巴拉汀治疗.采用简易精神状态检查量表（MMSE）评估认知功能,采用临床痴呆评定量表（CDR）评估痴呆程度,采用日常生活自理能力量表（ADL）评价日常活动能力;在治疗前和治疗12周后进行P300试验,测定P300潜伏期（P300-PL）和波幅（P300-A）;在治疗期间观察两组出现的药物不良反应.结果 治疗12周后,两组的MMSE均高于治疗前,两组的CDR、ADL均显著低于治疗前（t=8.353、18.289、6.627、2.463、10.681、5.201,均P〈0.05）;治疗12周后,观察组的MMSE[（23.17 ±3.95）分]均显著高于对照组[（19.88±4.43）分],观察组的CDR、ADL[（1.47±0.26）分、（39.87 ±7.43）分]均显著低于对照组[（1.80±0.34）分、（41.16 ±6.40）分]（t=4.823、6.725、5.278,均P〈0.05）.治疗12周后,两组的P300-PL均低于治疗前,两组的P300-A均显著高于治疗前（t=12.051、6.429、2.477、2.104,均P〈0.05）;治疗12周后,观察组的P300-PL[（321±39） ms]显著低于对照组[（356 ±44） ms]（t=5.180,P〈0.05）.在治疗过程中,对照组的不良反应发生率为9.59％,观察组的不良反应发生率为11.54％,差异无统计学意义（χ^2=0.151,P〉0.05）.结论 尼莫地平联合卡巴拉汀能有效治疗老年血管性痴呆,改善患者的认知功能、痴呆程度和日常活动能力,且药物不良反应发生率低,具有良好的临床应用价值.

Effect of Acupuncturing Houxi（S13）and Shenmen（HT7）in Trealing Cerebral Traumatio Dementia

Sixty-two cerebral traumatic patients were randomly divided into two groups: acupuncturegroup (32 cases) and physiotherapy group (30 cases) . The changes of mini-mental status examination(MMSE) scores and auditory evoked potential P300 were observed bofore and aiter treatment. Results: themarkedly effective rate and the total effective rate of the acupuncture group were 46. 9% and 81 . 3% respec-tively, which were significantly higher than that of 10. 0% and 30. 0% of the physiottierapy group (P < 0.01 ) . It was found that after treatment the above-mentioned indexes in the acupuncture group changed signifi-cantly (P < 0 . 05 ￣0. 001 ) , while in the physiotherapy group they were not(P >0. 05) . The therapeutic ef-fect in acupunctrue group was better than that in physiotherapy group (P< 0 . 001 ) . The results confirmed thatthe cognitive function of cerebral traumatic dementia patients was enhanced effectively after acupuncturingHouxi (S13 ) and Shenmen (HT7) .

Clinical study of acupuncture combined with medication for the elderly with Alzheimer disease

Objective:To observe the impact of mind-regulating acupuncture plus donepezil on the cognitive ability,mean cerebral blood flow velocity,event-related potential P300,and activities of daily living(ADL)in the aged patients with Alzheimer disease(AD).Methods:Sixty senile AD patients were divided into a treatment group and a control group following the envelope method for random allocation,with 30 cases in each group.Based on the conventional treatment of the internal medicine,the control group received oral donepezil,and the treatment group received oral donepezil plus mind-regulating acupuncture.After 4-week treatment,the two groups were evaluated by the mini-mental state examination(MMSE),Alzheimer disease assessment scale-cognitive part(ADAS-Cog),and ADL;changes in P300 and the mean cerebral blood flow velocity were also observed.Results:Before treatment,there were no significant differences in the scores of MMSE,ADAS-Cog,or ADL between the two groups(P>0.05).The MMSE score increased after treatment in both groups and was notably higher in the treatment group than in the control group,showing intra-group and inter-group statistical significance(P<0.05).After treatment,the ADAS-Cog and ADL scores dropped in both groups and were markedly lower in the treatment group than in the control group,also showing intra-group and inter-group statistical significance(P<0.05).Compared with the same group before treatment,the latency of P300 was shortened and the amplitude was extended in both groups,all with statistical significance(P<0.05);the latency was shorter and the amplitude was larger in the treatment group than in the control group after treatment,presenting significant between-group differences(P<0.05).The mean blood flow velocity accelerated after the intervention in both groups,and the differences were statistically significant(P<0.05);the improvement in the treatment group was more notable than that in the control group(P<0.05).Conclusion:Mind-regulating acupuncture plus donepezil can regulate the latency and amplitude of P300,increase cerebral blood flow,and improve the learning and memory abilities of AD patients.