Effect of Electroacupuncture on Expression of p53 Protein in Cerebral Cortex of Rats with Global Cerebral Ischemia/Reperfusion Injury

Objective: To observe the effect of electroacupuncture (EA) on expression of p53 protein in cerebral cortex of senile rats with global cerebral ischemia/reperfusion (IR) injury and to explore its mechanism. Methods: The cerebral IR injury rat model was established referring to Pulsinelli 4-vessel occlusion method. Thirty-six SD rats were randomly and evenly divided into the control group, the IR group and the IR plus EA (IR-EA) group. The animals in the control group were subjected to electrocauterization of vertebral arteries in bilateral flank orifice alone with the general carotid arteries unoccluded. To rats in the IR-EA group, immediately and 24h, 48h, 72h after cerebral IR, EA treatment on bilateral acupoint 'Zusanli' (ST36) was applied once a day, lasting for 60 minutes. After the final treatment, all the rats were sacrificed and their brains were taken to examine p53 protein expression by the immunohistochemical method. Results: Cells with positive p53 immunoreactivity in the cerebral cortex of rats in the IR group was significantly higher than that in the control group ( P<0. 05), while that in the IR-EA group was significantly lower than that in the IR group ( P<0. 05). Conclusion: EA could remarkably reduce expression of p53 protein in the cerebral cortex of senile rats with global cerebral IR injury, which might be one of the means for EA to inhibit neuronal ap-optosis after cerebral IR injury.

EXPRESSION OF P53 PROTEIN AND PROLIFERATING CELL NUCLEAR ANTIGEN IN HUMAN GESTATION TROPHOBLASTIC DISEASE

To study the relationship between p53 protein, proliferating cell nuclear antigen (PCNA) expression and benign or malignant gestational trophoblastic disease (MGTD). Methods: The histotomic sections of 48 patients with gestational trophoblastic disease and 24 patients of normal chorionic villi were stained using immunohistochemistry. The monoclonal antibodies were used to determine p53 protein and PCNA. Results: The frequency of p53 and PCNA positive expression were significantly different among the chorionic villi of normal pregnancy, hydratidiform mole (HM) and MGTD. But neither p53 nor PCNA has any relation with the clinical staging or metastasis of MGTD. Conclusion: Both P53 and PCNA are valuable in diagnosis of human gestational trophoblastic disease.

Correlation and Expression of COX-2 and P53 Protein in Basal Cell Carcinoma of Eyelid

The correlation between the expression of COX-2 and p53 protein in basal cell carcinoma （BCC） of eyelid and apoptosis was investigated. Specimens of BCC were collected from 40 cases （aged 28-68 y） at the Department of Pathology, Renmin Hospital of Wuhan University, and Department of Pathology, Zhongnan Hospital of Wuhan University during from 1999 to 2006. Five specimens of paracancerous tissues served as control group. Immunohistochemical staining was performed to detect the expression of COX-2 and p53 in the tissues. The average absorbance （A） and the average positive area rate of COX-2 and p53 protein were measured by image analysis. The positive area rate of COX-2 and p53 protein was analyzed by linear correlation analysis. It was found that COX-2 and p53 proteins were highly expressed in BCC of eyelid, and weakly expressed in paracancerous tissues. Image analysis revealed that the expression of COX-2 and p53 proteins in BCC of eyelid was sig- nificantly higher than that in paracancerous tissues （P〈0.01）. Spearman rank correlation analysis demonstrated a positive correlation between the expression of COX-2 and p53 （r=0.113, P=0.421）. It was concluded that COX-2 can increase the expression of p53 protein, therefore suppressing apoptosis.

Effects of nanometer particles and water decoction of the Chinese medicine mixture of pinellia ternate and scorpion on P53 protein contents and apoptosis in the cerebral cortex and hippocampus of epileptic rats

BACKGROUND： Water decoction of the Chinese traditional medicine mixture of pinellia ternate and scorpion is an effective treatment for epilepsy. OBJECTIVE： To compare nanometer particles and effects of water decoction of Chinese traditional medicine mixture on P53 protein levels and apoptosis in the cerebral cortex and hippocampus of epileptic rats. DESIGN, TIME AND SETTING： This randomized, controlled molecular biology study was performed at the Key Laboratory of Child Neural Rehabilitation of Jiamusi University from October to December 2007. MATERIALS： Forty healthy male Wistar rats were used in this study. Convulsion rat models were established by intraperitoneal infusion of 35 mg/kg pentylenetetrazol, once a day, for 28 successive days. The Chinese traditional medicine mixture, comprising pinellia ternate, scorpion, centipede, bupleurum, peach pit and glycyrrhiza, was purchased from Beijing Tongrentang, China. The mixture was made into nanometer particles and water decoction. The apoptosis determination kit and P53 immunohistochemistry kit were bought from Boster, China. METHODS： Forty Wistar rats were randomly divided into four groups of ten rats per group, control, nanometer particle, water decoction and epileptic model groups. Rats in the nanometer particle and water decoction groups were respectively treated with 300 mg/kg Chinese herb nanometer particle suspension and water decoction by gavage, once a day, for 28 days. Rats in the epileptic model group were fed an equal volume of saline by gavage. Rats in the control group were only administered with the same volume of saline by gavage. MAIN OUTCOME MEASURES： Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling （TUNEL） and immunohistochemistry were used to respectively detect neuronal apoptosis and P53 protein expression in the rat brain cortex and hippocampus at 28 days following administration. RESULTS： The number of apoptotic neurons was lower in the nanometer particle and water decoction groups compared with the epileptic model group. The number of apoptotic cells and P53 protein expression in the rat cerebral cortex and hippocampus was greater in the epileptic model group compared with the control group （P 〈 0.05）, but lower in the nanometer particle and water decoction groups compared with the epileptic model group （P 〈 0.05）, and lower in the nanometer particle group compared with the water decoction group （P 〈 0.05）. CONCLUSION： Chinese traditional medicine mixture decreases neuronal apoptosis and P53 protein expression in epileptic rats. The effect of nanometer particles is significant compared with water decoction.

THE STUDY ON RELATIONSHIP BETWEEN CIGARETTE SMOKING AND THE p53 PROTEIN AND P21 PROTEIN EXPRESSION IN NON-SMALL LUNG CANCER

This paper discusses the relationship between cigarette smoking and the p53 protein and P21 protein expression by the immunohistochemical analysis in 93 cases with lung cancer in which squamous cell carcinoma accounted for 45 cases, adenocarcinoma 48 cases. The results showed that positive proportion of p53 protein expression was 74.20% (28 of 37 squamous cell carcinoma, 21 of 30 adenocarcinomas) in cigarette smoking group with lung cancers, and 38.46% (3 of 8 squamous cell carcinoma, 7 of 18 adenocarcinomas) in nonsmoking group with lung cancers. The difference was statistically significant. Odds ratio was 4.14 and confidence limits for OR was 1.42-12.52. A dose-related presents in the p53 protein expression for the smoking amount and smoking years. The positive proportion of P21 protein expression was 79.31% (21 of 28 squamous cell carcinoma, 25 of 30 adenocarcinomas) in cigarette smoking group with lung cancers, and 82.75% (10 of 11 squamous, 14 of 18 adenocarcinomas) in nonsmoking group with lung cancers, the difference was not statistically significant. But their positive proportion of P21 protein expression were very high in both groups. It was indicated that no relationship between cigarette smoking and the P21 protein expression. We suggest that the p53 gene could be a common target of tobacco-associated carcinogenesis in lung cancer.

Association between HPV16/18 Infection and Expression of P53 protein in Laryngeal Papillomas

To understand the relationship between expression of P53 protein and HPV16/18 infection in laryngeal papillomas, PCR and immunohistochemical tech-niques were used to examine the paraffin-embedded tissue samples of laryngeal pa-pillomas from 35 subjects. HPV 16/18-DNA was found in 24 cases of laryngeal papillomas (68. 8 %). Overexpression of P53 protein was detected in 19 cases (54. 3 % ). Both HPV16/18-DNA and overexpression of P53 protein were demon-strated in 12 cases of laryngeal papillomas (34. 3 % ). Our results suggest that HPV16/l8 infection and P53 gene mutation are associated with pathogenesis of la-ryngeal papillomas. The relation between HPV infection and P53 mutation in tis-sues of laryngeal papillomas remains to be clarified.

ASSOCIATION OF EXPRESSION OF P53 PROTEIN WITH CELL PROLIFERATIVE ACTIVITY AND PROGNOSIS IN COLORECTAL CANCER

The expression of p53 protein in 66 cases ofcolorectal cancer and its relationship to cell porliferativeactivity, lymph node metastasis as well as prognosis wereinvestigated by means of AB-PAP immunohistochcmicaltechnique- The results showed that 62.1/o of colorectalcancer was positive of p53 expression. The cellproliferative activity and the frequency of lymph nodemetastasis in p53- positive tumors were significantlyhigher than those in p53-negative tumors (P<0.05). Thesurvival rate in patients with p53- positive tumors wassignificantly poorer than those with p53- negative tumors(P<0.05). Our results suggest that the abnormalexpression of p53 and cell proliferation associated withmutations are involved in both human carcinogenesis andlymph node metastasis of colorectal cancer. Examinationof p53 expression is of value in understanding the degreeof malignancy, and evaluating prognosis of the disease.

P53 protein expression and cell viability in irradiated peripheral blood mononuclear cells as bioindicators of radiosensitivity

Cellular radiosensitivity is directly correlated with the mechanism of DNA repair, in which p53 protein plays a major role. In this context, this study correlated cell death with p53 expression in lymphocytes irradiated in vitro with different doses of gammaradiation. For this, peripheral blood samples were collected from 10 healthy subjects. Each sample was divided in aliquots and, separately, irradiated with doses of 0,5;2 and 4 Gy. After this, peripheral blood mononuclear cells (PBMCs) were isolated and cultivated during 72 hours in 5% CO2 at 37oC without mitogen stimulation. The expression of p53 protein was evaluated by flow cytometry. In parallel, cell viability was determined by trypan blue staining. Statistical analysis was performed us-ing analysis of variance (ANOVA), differences were considered as statistically significant when p < 0.05. The results showed an increase of p53 expression with the absorbed dose, which was proportional to cell death, suggesting that p53 can be used as bioindicator of individual radiosensitivity.

Changes of p53 protein blood level in esophageal cancer patients and normal subjects from a high incidence area in Henan,China

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Relationship between therapeutic efficacy of arterial infusion chemotherapy and expression of P-glycoprotein and p53 protein in advanced hepatocellular carcinoma

瞄准：在先进肝细胞癌(HCC ) 调查在化学疗法的药功效和 P-glycoprotein (PGP ) 和 p53 蛋白质的表示之间的关系。方法：学习在 41 上被进行被对待由的有先进 HCC 的病人重复了动脉的注入化疗。从肿瘤的活体检视标本在所有病人在治疗的开始前被收集，并且标本被存储结冰直到免疫，到组织化学的染色，它在治疗的开始以后被执行检测 PGP 和 p53 蛋白质表情。41 个病人中的二十个与 anthracycline 药被对待(epirubicin 水疗院氯化物；anthracycline 组) ，并且留下 21 与 non-anthracycline 药被对待(在 11 个病人的 mitoxantrone 水疗院氯化物和在 10 个病人的 carboplatin；non-anthracycline 组) 。在化学疗法的功效和染色的免疫的结果之间的关系在二个组之间被比较。结果：在治疗的开始前， PGP 积极的率是 90.2%( 强烈积极， 36.6%) 并且蛋白质积极的率是的 p53 34.1%( 强烈积极， 19.5%) 。在 anthracycline 组，反应率是 40.0% 。显示出差的反应到治疗的病人的数字在有强烈积极的 PGP 表示(P=0.005 ) 的病人是显著地更大的，并且他们的预测是穷人(P=0.001 ) 。在 non-anthracycline 组，反应率是 42.9% ，并且在化学疗法的药功效和 PGP 或 p53 蛋白质表示之间没有重要关系。当仅仅从 11 个病人的数据与蒽醌对待药时， mitoxantrone，然而，被分析显示出差的反应到治疗的病人的数字在 p53 积极的病人(P=0.012 ) 之中是显著地更高的，不管幸存结果。结论：有为先进 HCC 的 anthracycline 药的化学疗法的功效能被免疫预言 PGP 的组织化学的分析表示。同样，染色评估 p53 蛋白质的免疫可能是有用的在与蒽醌药对待的病人预言反应。

THE QUANTITATIVE MEASUREMENT OF BCL-2, P53 PROTEIN AND PCNA EXPRESSION IN BREAST CARCINOMA AND THEIR CORRELATION WITH PROGNOSIS

To study quantitative index of bci-2, P53, Nroliferating cell nuclear antigen (PCNA),ER and PR in breast carcinoma and their correiation and their relatiousbip with prognosis, the ex expression of bcl-2, P53 and PCNA were studied by immunohistochemical technique. The measurementof ER and PR used enzyme linked affinuity histochemical methods. The quantitative index was analyzed by image technique. All analyses were hased on 60 breast carcinomas. The results were as follows:the more bcl-2 protein, the lower histological graded the longer survival term and the highersurvival rate (P< 0. 05). The quautitative measurement of bcl-2, P53 and PCNA expression were ofvalue in evaluating the degree of differentiation and prognosis in breast carcinoma. The quantitativeand qualitative measurement or p53 protein expression showed a Ⅰwerful evidence in evaluatingprognosis of bcl-2 were more significant in evaluating poor prognosis of breast carcinoma. A relationship between bcl-2 and ER, PR showed a better value for response to endocrine therapy in breastcarcinoma patients.

Clinical significance of immunohistochemica study of P53 protein in colorectal carcinoma

AIMS To determine the clinical significance of P53 protein ex-pression in colorectal carcinoma.METHODS The expression of P53 protein in 92 colorectal car-cinomas was examined using the monoclonal antibody PAb 1801.Correlation between P53 protein expression and prognosis in col-orectal carcinoma was analyzed using log-rank test.RESULTS The frequency of P53 protein expression was 57.61%,corresponding with Dukes’ staging.Analysis of survivordata demonstrated that the survival rate of colorectal carcinomawith positive P53 protein group was lower than that of negativeP53 protein group.CONCLUSIONS We conclude that the expression of P53 pro-tein is correlated with poor prognosis in colorectal carcinoma.

Human papillomavirus and p53 protein immunoreactivity in condylomata acuminatum and squamous cell carcinoma of penis

Aim: To determine the immunoreactive pattern of human papillomavirus (HPV) antigen and p53 protein in condylo-mata acuminatum (CA) and squamous cell carcinoma (SCC) of penis. Methods: Immunohistochemistry for HPVand p53 were performed in 40 specimens of formalin fixed, paraffin embedded tissues using a polyclonal (rabbit) anti-body against HPV and a monoclonal (mouse) antibody against human p53 protein. Twenty one cases of CA and nine-teen cases of SCC were examined. Results: HPV antigen was detected in all 21 CA and 2 penile SCC. p53 proteinoverexpression was observed in 12 of 19 (63%) SCC in which 6 cases were strong positive. Five of 21 CA (24%)showed low-grade p53 protein overexpression. Conclusion; CA is related to HPV infection and some cases showp53 protein low-grade overexpression. In contrast, p53 protein overexpression is common in penile SCC, which is sel-dom related to HPV infection. (Asian J Androl 2001 Mar; 3: 75-77)

EXPRESSION OF p53 PROTEIN IN CANCERS OF SMALL INTESTINE AND ITS RELATIONSHIP TO CLINICAL COURSE AND PROGNOSIS

In order to study the relationship of p53 gene mutation with the occurrence and prognosis of cancer of small intestine, expression of p53 protein was immunohistochemically examined. The results showed that p53 protein expression was high in 75% of small intestine cancer, and positive in 21.1% of the tissues close to cancer. In 7 cases of small intestinal adenoma, only one was immunoreactive. Sixteen samples of normal tissue of the intestine didn't show expression of p53protein. The study also found that the degree of p53protein expression was significantly correlated with that of tumor cell differentiation, invasion, metastasis and prognosis.

Apoptosis and P53 protein expression after rat spinal cord injury

objective: To determine the DNA damage, neuronal and glial cells apoptosis and expression of P53 protein in the rat spinal cord after compression injury. Methods: Terminal deoxynucleotidyl transferase . mediated dUPT nick end labeling (TUNEL), DNA gel electrophoresis and immunohistochemistry techniques were used to detect DNA fragmentation in the injured rat spinal cord. Results: The apoptosic cells and P53 protein presented at 4 h after spinal cord injury with a maximum presence at 24 h in the injuried T8.9 DNA fragmentation presented typical ladder pattern on agarose gel at 24 h. Conclusion: There are lots of neuronal and glial cells apoptosis with DNA damage after SCl. The P53 protein may play an important role in induc tion of neuronal and glial cells to apoptosis.

The Value of p21^(WAF1), p53 Protein and DNA Content in Large Intestine Cancer

目的 探讨 p2 1WAF1 蛋白和 p5 3蛋白在大肠癌发生发展中的作用及其临床意义。方法 对 4 1例大肠腺癌标本及 10例正常大肠粘膜标本 ,应用免疫组织化学S -P法检测细胞周期调控因子 p2 1WAF1 蛋白和突变型 p5 3蛋白 ,应用流式细胞术检测新鲜标本DNA含量。结果 p2 1WAF1 与 p5 3表达呈负相关 ;在正常大肠粘膜、高分化腺癌、中分化腺癌、低分化腺癌中 p5 3表达率逐渐增高 ,p2 1WAF1 表达率逐渐降低 ,SPF值逐渐升高 ;Dukes分期越晚 ,p2 1WAF1 表达率越低 ,p5 3表达率越高 ,SPF越高。结论 本实验证实了 p2 1WAF1 和 p5 3表达在肿瘤发生发展中起着调控点的作用 ;p2 1WAF1 、p5

乳腺癌超声图像特征与P53、PIK3CA蛋白表达状态相关性的研究

目的通过观测乳腺癌的超声图像特征,分析其与P53、PIK3CA蛋白表达的相关性。方法回顾性分析2022年1月—2023年10月本院经病理证实为乳腺癌的101例女性患者的临床资料,在获取病理前均行超声检查。二维超声用于观察乳腺癌大小、形态、边界、纵横比、后方回声以及有无微钙化,彩色多普勒超声用于观察病灶内部以及周边的血流情况。采用SP免疫组化法检测P53、PIK3CA蛋白的表达情况,分析其与超声图像特征的相关性。结果101例乳腺癌患者的临床病理资料显示,P53、PIK3CA的蛋白表达情况与乳腺癌病理亚型均具有相关性(χ^(2)=9.259、P=0.026,χ^(2)=7.927,P=0.048)。在超声图像特征中,血流强度、病灶后方回声衰减与P53蛋白表达具有相关性;病灶最长直径、病灶形态与PIK3CA蛋白表达具有相关性。P53蛋白表达阳性患者病灶血流信号丰富组(χ^(2)=8.191,P=0.004)和存在后方回声衰减组(χ^(2)=11.279、P<0.001)均高于阴性者,PIK3CA蛋白表达阳性患者声像图中的最长直径小于阴性者(t=2.132,P=0.036),而PIK3CA蛋白表达阳性患者病灶形态不规则比例(χ^(2)=4.551、P<0.033)高于阴性者。多因素Logistic回归表明,富血供是P53蛋白表达的影响因素,OR值为2.35[95%CI(1.11,5.74)];病灶形态不规则是PIK3CA蛋白表达的影响因素,OR值为4.58[95%CI(1.35,18.48)]。结论乳腺癌超声图像特征与P53、PIK3CA蛋白表达具有相关性,可以为临床诊疗乳腺癌提供重要的价值。

结直肠腺癌中错配修复蛋白缺失情况、p53表达情况及临床病理分析的研究

目的 探讨结直肠腺癌中错配修复蛋白缺失情况、p53表达情况及其与患者临床特征之间的关联性。方法 回顾性分析2021年1月至2022年1月眉山市人民医院收集的经过病理学检查确诊为结直肠腺癌患者282例的临床资料,分析错配修复蛋白缺失情况、p53表达情况及其与患者临床特征之间的关系;分析p53表达情况与错配修复蛋白缺失的相关性。结果 免疫组化发现,MLH1、MSH2、MSH6、PMS2的缺失数分别为32、21、17、39例,而p53的(+)、(-)、(+/-)表达分别为142、95、45例。部分错配修复蛋白的缺失与肿瘤最大径、肿瘤分化程度、T分期、有无淋巴结转移、有无神经累及、有无脉管累及存在关联(P<0.05);p53的表达与患者的肿瘤最大径、肿瘤分化程度、T分期、有无淋巴结转移、有无神经累及、有无脉管累及存在关联(P<0.05);结直肠腺癌中,p53与MLH1、PMS2存在相关性(P<0.05)。结论 结直肠腺癌患者的错配修复蛋白缺失情况、p53表达情况与患者临床病理特征存在关联性,且错配修复蛋白缺失情况和p53表达情况同样存在关联性。

USP7-MDM2-p53信号轴对子宫内膜癌细胞增殖、凋亡和细胞周期的影响

目的:探讨泛素特异性蛋白酶7(USP7)调节Mdm2 p53结合蛋白同源物(MDM2)-p53轴对子宫内膜癌细胞增殖、凋亡和细胞周期的影响。方法:Western blot检测人子宫内膜癌组织、癌旁组织、人子宫内膜上皮细胞hEEC及人子宫内膜癌细胞系Ishikawa、HEC-1-A、KLE中USP7蛋白表达。将Ishikawa细胞分为NC组、P22077(USP7抑制剂)组、pcDNA组、pcDNA-MDM2组、P22077+pcDNA组、P22077+pcDNA-MDM2组,CCK-8法和克隆形成实验检测Ishikawa细胞增殖;流式细胞术检测Ishikawa细胞凋亡与细胞周期变化;Western blot检测Ishikawa细胞中USP7、细胞周期蛋白D1(CyclinD1)、周期素依赖性激酶2(CDK2)、Bcl-2相关X蛋白(Bax)、MDM2、p53蛋白表达。以RG7388(MDM2抑制剂)或PFT-α(p53抑制剂)与20μmol/L P22077共处理Ishikawa细胞48 h以验证USP7-MDM2-p53信号轴上下游关系。结果:USP7蛋白在子宫内膜癌组织和细胞中高表达,且Ishikawa细胞中USP7蛋白表达量最高,因此,选择Ishikawa细胞为研究对象。与NC组比较,P22077组Ishikawa细胞OD 450值、克隆形成率、S期和G 2/M期细胞数、USP7、CyclinD1、CDK2、MDM2蛋白表达降低,细胞凋亡率、G_(0)/G_(1)期细胞数、p53、Bax蛋白表达升高(P<0.05);与NC组、pcDNA组比较,pcDNA-MDM2组Ishikawa细胞OD 450值、克隆形成率、S期和G 2/M期细胞数、USP7、CyclinD1、CDK2、MDM2蛋白表达升高,细胞凋亡率、G_(0)/G_(1)期细胞数、p53、Bax蛋白表达降低(P<0.05);与P22077组、P22077+pcDNA组比较,P22077+pcDNA-MDM2组Ishikawa细胞OD 450值、克隆形成率、S期和G 2/M期细胞数、USP7、CyclinD1、CDK2、MDM2蛋白表达升高,细胞凋亡率、G_(0)/G_(1)期细胞数、p53、Bax蛋白表达降低(P<0.05)。p53为USP7-MDM2通路下游分子。结论:抑制USP7表达可能通过下调MDM2来激活p53进而抑制Ishikawa细胞增殖、促进细胞凋亡及周期停滞。