The unripe palmleaf raspberry,namely Fupenzi(FPZ),is an important medicinal and edible food.This study aims to evaluate the potential of FPZ extracts prepared with different approaches in attenuating hyperglycemia,gou...The unripe palmleaf raspberry,namely Fupenzi(FPZ),is an important medicinal and edible food.This study aims to evaluate the potential of FPZ extracts prepared with different approaches in attenuating hyperglycemia,gout,Alzheimer’s disease,and pigmentation,to obtain the enriching fraction and to identify the major active compounds.Results indicated that FPZ extracts showed weak activity against acetylcholinesterase,considerable ability against tyrosinase and xanthine oxidase,but excellent inhibition onα-glucosidase.Ultrasound-assisted 40%ethanol extract(40EUS)gave the highest phenolics content,and the bestα-glucosidase inhibition(IC_(50)=0.08μg/mL),which is 877-fold higher than that of positive control acarbose.The 40%ethanol eluting fraction of 40EUS showed the strongestα-glucosidase inhibition with the IC_(50) value of 37.79 ng/mL,it could also effectively attenuate the fasting blood glucose level and oral glucose tolerance of C57BL/6 mice.Twenty-six compounds were identified from 40%ethanol fraction by using HPLC-QTOF-MS/MS,hydrolysable tannins(including 11 ellagitannins and 4 gallotannins)were the major compounds,phenolic acids came to the second.Above results could provide important technical supporting for the further application and research of FPZ in health foods and drugs against diabetes.展开更多
目的 研究不同 p H值对东北红豆杉和南方红豆杉愈伤组织生长和紫杉醇含量的影响。方法 采用不同p H值的 B5培养基培养两种红豆杉愈伤组织 ,测定生长率、PAL 活性和紫杉醇含量。结果 不同红豆杉愈伤组织所需最适 p H值不同 ,而有利于...目的 研究不同 p H值对东北红豆杉和南方红豆杉愈伤组织生长和紫杉醇含量的影响。方法 采用不同p H值的 B5培养基培养两种红豆杉愈伤组织 ,测定生长率、PAL 活性和紫杉醇含量。结果 不同红豆杉愈伤组织所需最适 p H值不同 ,而有利于愈伤组织生长的 p H值均不利于 PAL 活性的提高和紫杉醇的积累。即 :愈伤组织生长快时 ,PAL活性将下降 ,紫杉醇含量也减少。结论 p H值明显影响红豆杉愈伤组织的生长及次级代谢水平 。展开更多
基金the financial support of National Natural Science Foundation of China(31860475)Key Youth Foundation of Jiangxi Province(20192ACB21011)Jiangxi“Shuangqian”Program(JXSQ2018101008).
文摘The unripe palmleaf raspberry,namely Fupenzi(FPZ),is an important medicinal and edible food.This study aims to evaluate the potential of FPZ extracts prepared with different approaches in attenuating hyperglycemia,gout,Alzheimer’s disease,and pigmentation,to obtain the enriching fraction and to identify the major active compounds.Results indicated that FPZ extracts showed weak activity against acetylcholinesterase,considerable ability against tyrosinase and xanthine oxidase,but excellent inhibition onα-glucosidase.Ultrasound-assisted 40%ethanol extract(40EUS)gave the highest phenolics content,and the bestα-glucosidase inhibition(IC_(50)=0.08μg/mL),which is 877-fold higher than that of positive control acarbose.The 40%ethanol eluting fraction of 40EUS showed the strongestα-glucosidase inhibition with the IC_(50) value of 37.79 ng/mL,it could also effectively attenuate the fasting blood glucose level and oral glucose tolerance of C57BL/6 mice.Twenty-six compounds were identified from 40%ethanol fraction by using HPLC-QTOF-MS/MS,hydrolysable tannins(including 11 ellagitannins and 4 gallotannins)were the major compounds,phenolic acids came to the second.Above results could provide important technical supporting for the further application and research of FPZ in health foods and drugs against diabetes.
文摘目的 研究不同 p H值对东北红豆杉和南方红豆杉愈伤组织生长和紫杉醇含量的影响。方法 采用不同p H值的 B5培养基培养两种红豆杉愈伤组织 ,测定生长率、PAL 活性和紫杉醇含量。结果 不同红豆杉愈伤组织所需最适 p H值不同 ,而有利于愈伤组织生长的 p H值均不利于 PAL 活性的提高和紫杉醇的积累。即 :愈伤组织生长快时 ,PAL活性将下降 ,紫杉醇含量也减少。结论 p H值明显影响红豆杉愈伤组织的生长及次级代谢水平 。
