A new asymmetric bidentate copper（Ⅱ） complex,CuL 2（HL=2-（（E）-（4-bromophenylimino）methyl）-6-bromo-4-chlorophenol）,has been synthesized and characterized by elemental analyses and single-crystal X-ray diffrac...A new asymmetric bidentate copper（Ⅱ） complex,CuL 2（HL=2-（（E）-（4-bromophenylimino）methyl）-6-bromo-4-chlorophenol）,has been synthesized and characterized by elemental analyses and single-crystal X-ray diffraction.The complex crystallizes in the monoclinic space group P2 1 /c with a=11.218（3）,b=9.355（3）,c=13.449（4）,β=108.722（4）°,V=1336.8（6）3,Z=2,Dc=2.008 g/cm 3,μ（MoKα）=7.024 mm-1,F（000）=806,S=0.999,the final R=0.0342 and wR=0.0641for2611observed reflections （I〉2σ（I））.The central copper（Ⅱ） is four-coordinate and bonds to two nitrogen and two oxygen atoms from two Schiff base ligands.The complex is linked into a two-dimensional supramolecular structure by weak intermolecular interactions.In addition,DNA-binding properties of the metal complex were investigated using spectrometric titrations and viscosity measurements.The results show that the complex binds with calf-thymus DNA（CT-DNA）,presumably via a partial intercalative mode.The intrinsic binding constant of the Cu（Ⅱ） complex with DNA is 7.335×10 3 M-1.展开更多
Two new complexes： [Cu（TBZ）（bipy）Cl]Cl[H2O （1） and [Cu（TBZ）（phen）CI]CloH20 （2） [TBZ=2-（4＇-thiazolyl）- benzimidazole, phen = 1,10-phenanthroline and bipy =2,2＇-bipyridine] have been synthesized and cha...Two new complexes： [Cu（TBZ）（bipy）Cl]Cl[H2O （1） and [Cu（TBZ）（phen）CI]CloH20 （2） [TBZ=2-（4＇-thiazolyl）- benzimidazole, phen = 1,10-phenanthroline and bipy =2,2＇-bipyridine] have been synthesized and characterized by elemental analysis, molar conductivity, IR, and UV-vis methods. Complex 2, structurally characterized by single-crystal X-ray crystallography, crystallizes in the monoclinic space group P21/c in a unit cell of a = 0.85257（12） nm, b=2.5358（4） nm, c=1.15151（13） nm, β=118.721（8）°, V=2.183.2（5） nm^3, Z=4, Dc=1.624 gocm 3, μ= 1.367 mm^-1. The complexes, free ligands and chloride copper（II） salt were each tested for their ability to inhibit the growth of two gram-positive （B. subtilis and S. aureus） and two gram-negative （Salmonella and E. coli） bacteria. The complexes showed good antibacterial activities against the microorganisms. The interaction between the com- plexes and calf thymus DNA in aqueous solution was investigated adopting electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and cyclic voltammetry. Results suggest that the two complexes can bind to DNA by intercalative mode. In addition, the result of agarose gel electrophoresis suggested that the complexes can cleave the plasmid DNA at physiological pH and room temperature. Mechanistic studies with different inhibiting reagents reveal that hydroxyl radicals, and a singlet oxygen-like copper-oxo species are all involved in the DNA scission process mediated by the complexes.展开更多
A new ternary complex [Cu（PBT）（Gly）（H2O）]ClO4 （PBT=2-（2＇-pyridyl）benzothiazole, and Gly=glycinate） has been synthesized and characterized by elemental analysis, molar conductivity, and IR methods. The compl...A new ternary complex [Cu（PBT）（Gly）（H2O）]ClO4 （PBT=2-（2＇-pyridyl）benzothiazole, and Gly=glycinate） has been synthesized and characterized by elemental analysis, molar conductivity, and IR methods. The complex, structurally characterized by single-crystal X-ray crystallography, shows a slightly distorted square-pyramidal coordination geometry in which two nitrogen atoms of PBT and the carboxylate oxygen atom O and the amino nitrogen atom N of Gly bind at the basal plane, a water molecule is coordinated at the apical position. The complex, Cu（ClO4）2 and flee PBT were each tested for their ability to inhibit the growth of two Gram（--） （Escherichia coil, Salmonella） and two Gram（＋） （Bacillus subtilis, Staphylococcus aureus） microorganisms. The complex showed good antibacterial activities against the microorganisms compared with free PBT and Cu（C104）2. The interaction between the complex and calf thymus DNA in aqueous solution was investigated using electronic spectroscopy, fluorescence spectroscopy, circular dichroic spectroscopy, and viscosity measurements. Results suggest that the complex could bind to DNA by an intercalative mode. In addition, the result of agarose gel electrophoresis showed that the complex can cleave the plasmid DNA by hydroxyl radicals and singlet oxygen-like species under the condition of physiological pH and room temperature.展开更多
文摘A new asymmetric bidentate copper（Ⅱ） complex,CuL 2（HL=2-（（E）-（4-bromophenylimino）methyl）-6-bromo-4-chlorophenol）,has been synthesized and characterized by elemental analyses and single-crystal X-ray diffraction.The complex crystallizes in the monoclinic space group P2 1 /c with a=11.218（3）,b=9.355（3）,c=13.449（4）,β=108.722（4）°,V=1336.8（6）3,Z=2,Dc=2.008 g/cm 3,μ（MoKα）=7.024 mm-1,F（000）=806,S=0.999,the final R=0.0342 and wR=0.0641for2611observed reflections （I〉2σ（I））.The central copper（Ⅱ） is four-coordinate and bonds to two nitrogen and two oxygen atoms from two Schiff base ligands.The complex is linked into a two-dimensional supramolecular structure by weak intermolecular interactions.In addition,DNA-binding properties of the metal complex were investigated using spectrometric titrations and viscosity measurements.The results show that the complex binds with calf-thymus DNA（CT-DNA）,presumably via a partial intercalative mode.The intrinsic binding constant of the Cu（Ⅱ） complex with DNA is 7.335×10 3 M-1.
文摘Two new complexes： [Cu（TBZ）（bipy）Cl]Cl[H2O （1） and [Cu（TBZ）（phen）CI]CloH20 （2） [TBZ=2-（4＇-thiazolyl）- benzimidazole, phen = 1,10-phenanthroline and bipy =2,2＇-bipyridine] have been synthesized and characterized by elemental analysis, molar conductivity, IR, and UV-vis methods. Complex 2, structurally characterized by single-crystal X-ray crystallography, crystallizes in the monoclinic space group P21/c in a unit cell of a = 0.85257（12） nm, b=2.5358（4） nm, c=1.15151（13） nm, β=118.721（8）°, V=2.183.2（5） nm^3, Z=4, Dc=1.624 gocm 3, μ= 1.367 mm^-1. The complexes, free ligands and chloride copper（II） salt were each tested for their ability to inhibit the growth of two gram-positive （B. subtilis and S. aureus） and two gram-negative （Salmonella and E. coli） bacteria. The complexes showed good antibacterial activities against the microorganisms. The interaction between the com- plexes and calf thymus DNA in aqueous solution was investigated adopting electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and cyclic voltammetry. Results suggest that the two complexes can bind to DNA by intercalative mode. In addition, the result of agarose gel electrophoresis suggested that the complexes can cleave the plasmid DNA at physiological pH and room temperature. Mechanistic studies with different inhibiting reagents reveal that hydroxyl radicals, and a singlet oxygen-like copper-oxo species are all involved in the DNA scission process mediated by the complexes.
基金We are thankful for the Natural Science Foundation of Guangdong Province （No. 10151064201000016） and the 211 Project Program Foundation of South China Agricultural University （No. 2009B010100001）.
文摘A new ternary complex [Cu（PBT）（Gly）（H2O）]ClO4 （PBT=2-（2＇-pyridyl）benzothiazole, and Gly=glycinate） has been synthesized and characterized by elemental analysis, molar conductivity, and IR methods. The complex, structurally characterized by single-crystal X-ray crystallography, shows a slightly distorted square-pyramidal coordination geometry in which two nitrogen atoms of PBT and the carboxylate oxygen atom O and the amino nitrogen atom N of Gly bind at the basal plane, a water molecule is coordinated at the apical position. The complex, Cu（ClO4）2 and flee PBT were each tested for their ability to inhibit the growth of two Gram（--） （Escherichia coil, Salmonella） and two Gram（＋） （Bacillus subtilis, Staphylococcus aureus） microorganisms. The complex showed good antibacterial activities against the microorganisms compared with free PBT and Cu（C104）2. The interaction between the complex and calf thymus DNA in aqueous solution was investigated using electronic spectroscopy, fluorescence spectroscopy, circular dichroic spectroscopy, and viscosity measurements. Results suggest that the complex could bind to DNA by an intercalative mode. In addition, the result of agarose gel electrophoresis showed that the complex can cleave the plasmid DNA by hydroxyl radicals and singlet oxygen-like species under the condition of physiological pH and room temperature.
