随着国际标准化心脏死亡器官捐献(donation after cardiac death, DCD)工作的广泛开展,DCD来源的供肾在临床的应用逐渐增多。DCD供肾移植术后并发症亦呈逐渐增加趋势,如移植肾缺血再灌注损伤、机会性病毒感染、排斥反应等,这就要求临床...随着国际标准化心脏死亡器官捐献(donation after cardiac death, DCD)工作的广泛开展,DCD来源的供肾在临床的应用逐渐增多。DCD供肾移植术后并发症亦呈逐渐增加趋势,如移植肾缺血再灌注损伤、机会性病毒感染、排斥反应等,这就要求临床医师评估供肾质量,除了临床评分及肉眼评估外,肾移植术中零点穿刺标本是评估移植肾功能、预测器官分配或远期存活的金标准[1]。展开更多
[Objectives]To investigate the spasticity of rat tail and the expression of dopamine receptor-1(DRD1)mRNA in the spinal cord after spinal cord injury(SCI)induced tail spasticity in rats.[Methods]Adult male Wistar rats...[Objectives]To investigate the spasticity of rat tail and the expression of dopamine receptor-1(DRD1)mRNA in the spinal cord after spinal cord injury(SCI)induced tail spasticity in rats.[Methods]Adult male Wistar rats were randomly divided into Sham group and SCI group.The second sacral spinal cord(S2)segment of SCI rats was completely transected.60 d after operation,the rat tail spasticity was scored,and then the spinal cord tissues below the level of S2 spinal cord transection were taken.The expression of DRD1 mRNA in the sacrococcygeal spinal cord was detected by qPCR.In addition,3 normal rats were used for DAR/neuronal nuclei(NeuN)and DRD1/choline acetyltransferase(ChAT)immunofluorescence staining to study the distribution of DRD1 in spinal cord and the properties of DRD1 positive cells.[Results]60 d after operation in SCI group,the tail spasticity of rats developed fully,and the symptoms of spasticity were typical.qPCR results showed that the expression of DRD1 mRNA in SCI group was significantly lower than that in Sham group(P<0.05).DRD1 was widely distributed in the dorsal horn,intermediate zone and ventral horn at the sacrococcygeal end of the rat spinal cord.[Conclusions]The decrease of DRD1 mRNA expression after SCI may be related to the occurrence and development of spasticity.展开更多
文摘随着国际标准化心脏死亡器官捐献(donation after cardiac death, DCD)工作的广泛开展,DCD来源的供肾在临床的应用逐渐增多。DCD供肾移植术后并发症亦呈逐渐增加趋势,如移植肾缺血再灌注损伤、机会性病毒感染、排斥反应等,这就要求临床医师评估供肾质量,除了临床评分及肉眼评估外,肾移植术中零点穿刺标本是评估移植肾功能、预测器官分配或远期存活的金标准[1]。
基金Supported by Youth Project of National Natural Science Foundation of China(81501080)Funding Project for Introducing Overseas Students in Hebei Provincial Department of Human Resources and Social Security(C20190174)Construction of Chengde Biomedical Industry Research Institute(202205B086)。
文摘[Objectives]To investigate the spasticity of rat tail and the expression of dopamine receptor-1(DRD1)mRNA in the spinal cord after spinal cord injury(SCI)induced tail spasticity in rats.[Methods]Adult male Wistar rats were randomly divided into Sham group and SCI group.The second sacral spinal cord(S2)segment of SCI rats was completely transected.60 d after operation,the rat tail spasticity was scored,and then the spinal cord tissues below the level of S2 spinal cord transection were taken.The expression of DRD1 mRNA in the sacrococcygeal spinal cord was detected by qPCR.In addition,3 normal rats were used for DAR/neuronal nuclei(NeuN)and DRD1/choline acetyltransferase(ChAT)immunofluorescence staining to study the distribution of DRD1 in spinal cord and the properties of DRD1 positive cells.[Results]60 d after operation in SCI group,the tail spasticity of rats developed fully,and the symptoms of spasticity were typical.qPCR results showed that the expression of DRD1 mRNA in SCI group was significantly lower than that in Sham group(P<0.05).DRD1 was widely distributed in the dorsal horn,intermediate zone and ventral horn at the sacrococcygeal end of the rat spinal cord.[Conclusions]The decrease of DRD1 mRNA expression after SCI may be related to the occurrence and development of spasticity.