[Objective] The aim was to establish the optimal rye-specific PCR reaction system for rye.[Method] The ordinary wheat ＂Chinese Spring＂,S165,rye,octoploid triticale and hexaploid triticale were used as materials to c...[Objective] The aim was to establish the optimal rye-specific PCR reaction system for rye.[Method] The ordinary wheat ＂Chinese Spring＂,S165,rye,octoploid triticale and hexaploid triticale were used as materials to carry out study on the effect of the amount of template DNA,primers,dNTPs,Mg2＋ concentrations,Taq DNA polymerase and annealing temperature on the rye-specific PCR reaction system of rye.[Result] The genomic DNA extracted by modified CTAB DNA extraction method showed high quality,which was satisfied for the PCR reaction template.The rye-specific PCR reaction system was 25 μl,including 10 × buffer solution,1.5 mmol/L MgCl2,200 μmol/L dNTP,40 ng primers,40-60 ng DNA template and 1 U Taq DNA polymerase.[Conclusion] The optimal rye-specific PCR reaction system was established,which provided basis for the identification of exogenous germplasm of rye in wheat background.展开更多
Rice false smut is a destructive disease that affects rice grain badly.The disease seriously affects the yield and quality of rice in Heilongjiang Province.In this paper,a pair of specific primers was designed to dete...Rice false smut is a destructive disease that affects rice grain badly.The disease seriously affects the yield and quality of rice in Heilongjiang Province.In this paper,a pair of specific primers was designed to detect the false smut pathogen rapidly and efficiently.The results showed that the pair of primers had strong specificity for false smut pathogen.In addition,the sensitivity of this primer to the genomic DNA of rice false smut pathogen in PCR reaction was 1 pg.By using these primers,the rice false smut pathogen could be detected within 48 h after inoculation,and a PCR reaction system with good specificity and high sensitivity was established.展开更多
基金Supported by Young Academic Backbone Support Program of Heilongjiang Province(1152G047)Student Innovation Project of Mudanjiang Normal University~~
文摘[Objective] The aim was to establish the optimal rye-specific PCR reaction system for rye.[Method] The ordinary wheat ＂Chinese Spring＂,S165,rye,octoploid triticale and hexaploid triticale were used as materials to carry out study on the effect of the amount of template DNA,primers,dNTPs,Mg2＋ concentrations,Taq DNA polymerase and annealing temperature on the rye-specific PCR reaction system of rye.[Result] The genomic DNA extracted by modified CTAB DNA extraction method showed high quality,which was satisfied for the PCR reaction template.The rye-specific PCR reaction system was 25 μl,including 10 × buffer solution,1.5 mmol/L MgCl2,200 μmol/L dNTP,40 ng primers,40-60 ng DNA template and 1 U Taq DNA polymerase.[Conclusion] The optimal rye-specific PCR reaction system was established,which provided basis for the identification of exogenous germplasm of rye in wheat background.
基金Supported by the Science and Technology Precision Poverty Alleviation Project of Planting Industry(ZY18C08)Special Project to Guide the Development of Central and Local Science and Technology。
文摘Rice false smut is a destructive disease that affects rice grain badly.The disease seriously affects the yield and quality of rice in Heilongjiang Province.In this paper,a pair of specific primers was designed to detect the false smut pathogen rapidly and efficiently.The results showed that the pair of primers had strong specificity for false smut pathogen.In addition,the sensitivity of this primer to the genomic DNA of rice false smut pathogen in PCR reaction was 1 pg.By using these primers,the rice false smut pathogen could be detected within 48 h after inoculation,and a PCR reaction system with good specificity and high sensitivity was established.
